scholarly journals THE EFFECT OF SOME GROWTH STIMULATORS AND INHIBITORS ON SEED GERMINATION OF PAPAVER SOMNIFERUM

1970 ◽  
Vol 63 ◽  
Author(s):  
Ana – Maria Popa ◽  
Alina Trofin ◽  
C. Leonte
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Growth Regulators ◽  
Papaver Somniferum ◽  
Metabolic Process ◽  
Opium Poppy ◽  
Large Area ◽  
Phenoxy Acetic Acid ◽  
Inhibition Of Growth ◽  
Dichlorophenoxy Acetic Acid

The purpose of this work is to pinpoint the influence of some growth regulators, stimulators and inhibitors (the Na salt of 2-Cl, 4-sulfonamido phenoxy -acetic acid, the Na salt of 4-Cl, 2-sulfonamido phenoxy -acetic acid and 2, 4 dichlorophenoxy – acetic acid) on the process of germination of opium poppy (Papaver somniferum L., var. Botosani). The substances have a large area of action, there effects being felt both on entire plants and on certain parts and tissues, thus regulating some important metabolic process. The stimulation and inhibition of growth regulators on germination depend on utilized variant of treatment.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

Interactions between Herbicidal Carbamates and Growth Regulators

Weed Science ◽  
1970 ◽  
Vol 18 (1) ◽  
pp. 137-139 ◽  
Author(s):  
C. S. James ◽  
G. N. Prendeville ◽  
G. F. Warren ◽  
M. M. Schreiber
Keyword(s):  
Acetic Acid ◽  
Glycine Max ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Amaranthus Retroflexus ◽  
Differential Movement ◽  
Anisic Acid ◽  
Polygonum Lapathifolium ◽  
Whole Plants ◽  
Dichlorophenoxy Acetic Acid

Interactions between carbamate and growth regulator herbicides were antagonistic both in whole plants and in plant segments. When combinations of isopropylm-chlorocarbanilate (chlorpropham) and (2,4-dichlorophenoxy)acetic acid (2,4-D) were applied to the foliage of either redroot pigweed (Amaranthus retroflexusL.) or pale smartweed (Polygonum lapathifoliumL.), the severe twisting effects of 2,4-D were greatly reduced. This interaction did not involve differential movement or metabolism of either herbicide. The induced elongation of soybean hypocotyl sections by the three growth regulators 2,4-D, 3,6-dichloro-o-anisic acid (dicamba), and 4-amino-3,5,6-trichloropicolinic acid (picloram) was inhibited in the presence of either chlorpropham orS-ethyl dipropylthiocarbamate (EPTC). Similarly, curvature tests using soybean (Glycine max(L.) Merr.) hypocotyl sections showed the curvature induced by the growth regulators to be almost completely eliminated by the presence of the carbamates.

scholarly journals Improvement of Seed Germination in Three Medicinal Plant Species by Plant Growth Regulators

HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 887-891 ◽  
Author(s):  
Khalid M. Elhindi ◽  
Yaser Hassan Dewir ◽  
Abdul-Wasea Asrar ◽  
Eslam Abdel-Salam ◽  
Ahmed Sharaf El-Din ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Rate ◽  
Germination Percentage ◽  
Mentha Piperita ◽  
Naphthalene Acetic Acid ◽  
Sweet Basil

Peppermint (Mentha piperita), sweet basil (Ocimum basilicum), and coriander (Coriandrum sativum) are important medicinal plants in the pharmacological industry. These plants are produced in commercial scale but their seeds exhibit low germination percentages under favorable germination conditions. Enhancing seed germination is thus crucial for improving the production of these plants. The influence of gibberellic acid (GA3), indole-3-acetic acid (IAA), indol-3-butyric acid (IBA), and naphthalene acetic acid (NAA) on seed germination of the three plants were investigated. The seeds were soaked in each plant growth regulator at 50, 100, and 150 mg·L−1 for 24 hours at 25 ± 2 °C. Seed germination was checked daily for 20 days and germination parameters including final germination percentage (FGP), corrected germination rate (CGRI), and number of days lapsed to reach 50% of FGP (GT50) were recorded. The phosphorus and protein contents were determined in germinated seedlings on day 21 of culture. All plant growth regulators enhanced seed germination as compared with control. However, GA3 improved seed germination more than IAA, IBA, and NAA. GA3 at 100 mg·L−1 significantly increased the FGP from 22.3% and 33.3% (control) to 74% and 65.6% for peppermint and sweet basil, respectively. Low concentration of GA3 at 50 mg·L−1 increased the FGP for coriander from 27% to 52.3%. GA3 also increased CGRI, GT50, phosphorus, and protein contents in germinated seedlings as compared with control. Seeds of peppermint, sweet basil, and coriander possess a physiological dormancy that could be elevated by GA3 presowing treatment. This study established a successful methodology for optimizing seed germination to satisfy the demand for the medicinal parts of these plants in the pharmacological industry.

scholarly journals Plant Regeneration of Pampas Grass from Immature Inflorescences Cultured in Vitro

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 841-843 ◽  
Author(s):  
C.D. Robacker ◽  
W.L. Corley
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Developmental Stage ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Basal Medium ◽  
Cortaderia Selloana ◽  
Dichlorophenoxy Acetic Acid

A micropropagation system to obtain plants from inflorescences of pampas grass (Cortaderia selloana Schult. `Pumila') was developed. Factors examined included developmental stage of inflorescence cultured and growth regulator combinations and concentrations that support explant establishment, shoot regeneration, and rooting. Immature inflorescences ≈300 mm long formed many shoot primordia when initially cultured on Murashige and Skoog basal medium containing 4.5 μm 2,4-D and 8.9 μm BA and subcultured to medium with 0.4 μm 2,4-D and 4.4 μm BA. Thereafter, monthly transfer to a medium without growth regulators yielded about three shoots per tube per month for more than 6 months. Most shoots rooted spontaneously and were easily hardened to greenhouse conditions. Field-tested plants flowered within 2 years and nearly all appeared identical to the parent cultivar. With this technique, several thousand plants can be obtained from a single inflorescence in 1 year. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA); (2,4-dichlorophenoxy)acetic acid (2,4-D).

RNA Synthesis as the Basis for EPTC and 2,4-D Antagonism

Weed Science ◽  
1972 ◽  
Vol 20 (1) ◽  
pp. 8-11 ◽  
Author(s):  
C. E. Beste ◽  
M. M. Schreiber
Keyword(s):  
Acetic Acid ◽  
Ribosomal Rna ◽  
Preliminary Analysis ◽  
Rna Synthesis ◽  
Selective Inhibition ◽  
Acid Synthesis ◽  
Inhibition Of Growth ◽  
Dichlorophenoxy Acetic Acid ◽  
Soybean Tissue ◽  
Hypocotyl Segments

Three-day-old soybean(Glycine max(L.) Merr., ‘Hawkeye 63′) hypocotyl segments (0.5 to 1.5 cm below the cotyledons) were utilized to evaluate the effects of the antagonistic interaction ofS-ethyl dipropylthiocarbamate (EPTC) and (2,4-dichlorophenoxy)acetic acid (2,4-D) on growth and nucleic acid synthesis. EPTC inhibited growth and RNA synthesis in soybean tissue. The addition of 2,4-D with EPTC was antagonistic to EPTC inhibition of growth and caused an increase in total RNA synthesis. Analysis of soybean tissue nucleic acids by methylated-albumin-kieselguhr(MAK) column chromatography showed that EPTC inhibited ribosomal-RNA (r-RNA), DNA like RNA (D-RNA), and tenaciously-bound-RNA (TB-RNA) synthesis. The combination of 2,4-D with EPTC caused an increase in D-RNA and TB-RNA synthesis compared to the EPTC treatment alone. The 2,4-D-enhanced synthesis of D-RNA and TB-RNA in the presence of EPTC appears to be the basis of the antagonism between EPTC and 2,4-D. Preliminary analysis of r-RNA indicated that EPTC preferentially inhibited the synthesis of 18S r-RNA more than 25S r-RNA and that 2,4-D had no effect on this selective inhibition.

scholarly journals Effects of Plant Growth Regulator on Yield and Economic Benefit of Sweet Pepper (Capsicum annum L.)

2018 ◽  
Vol 16 (02) ◽  
pp. 58-64
Author(s):  
S Akhter ◽  
T Mostarin ◽  
K Khatun ◽  
F Akhter ◽  
A Parvin
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Sweet Pepper ◽  
Net Income ◽  
Treatment Combination ◽  
Phenoxy Acetic Acid ◽  
Capsicum Annum

The experiment was conducted in the Horticultural Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Plant growth regulators were applied which had significant effect on yield of sweet pepper (Capsicum annum L.). The experiment consisted of two factors. Factor A: Plant growth regulators (four levels) as G0: Control, G1: Gibberellic Acid (GA3) @ 30 ppm, G2: 4-Chloro Phenoxy Acetic Acid (4-CPA) @ 45 ppm and G3: 4-Chloro Phenoxy Acetic Acid (4-CPA) @ 45 ppm + Gibberellic Acid (GA3) @ 30 ppm and Factor B: Number of spray (three levels) as N0: Control (no spray), N1: two spray, N2: three spray. In case of plant growth regulators, the highest yield (27.77 t/ha) was found from G3 treatment, whereas the lowest (18.87 t/ha) was from G0 treatment. For number of spray the maximum yield (26.0 t/ha) was recorded from N2 treatment, while the minimum yield (19.87 t/ha) was from N0 treatment. The results indicated that the highest yield (31.8 t/ha) was observed from G3N2 treatment combination, while the lowest yield (17.5 t/ha) was from G0N0 treatment combination. Due to combined effect, the highest yield (31.8 t/ha) with net income (Tk/ha 1416558) and BCR (2.46) was observed from G3N2 treatment combination, while the lowest yield (17.5 t/ha) with net income (Tk/ha 433045) and BCR (1.49) from G0N0 treatment combination. Thus, three times spray with (4- Chloro Phenoxy Acetic Acid + Gibberellic Acid) may be recommended for achieving the higher growth, yield and economic benefit of sweet pepper. The Agriculturists 2018; 16(2) 58-64

In vitro studies to produce double haploid in Indica hybrid rice

Biologia ◽  
2011 ◽  
Vol 66 (6) ◽  
Author(s):  
Piyachai Premvaranon ◽  
Suchada Vearasilp ◽  
Sa-nguansak Thanapornpoonpong ◽  
Dumnern Karladee ◽  
Shela Gorinstein
Keyword(s):  
Acetic Acid ◽  
Organic Compounds ◽  
Growth Regulators ◽  
Double Haploid ◽  
Activated Charcoal ◽  
Hybrid Rice ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxy Acetic Acid ◽  
Indica Hybrid Rice

AbstractThe aim of this investigation was to improve in vitro the technique of production of double haploid in Indica hybrid rice by combining anther culture, hormone shock and doubling chromosome. It was discussed how to avoid somaclonal variation during culturing and to reduce the time of this process. The anthers of KDML 105 × SPR 1 (Indica × Indica) were cultured in Linsmaier and Skoog (LS) medium, which contained nutrients, growth regulators [(2,4,-dichlorophenoxy acetic acid (2,4-D) and naphthalene acetic acid (NAA)] and organic compounds, and then subcultured by inducing embryo-like structure (ELS) LS media. During 4 weeks used LS media supplemented with 10 μM KNO3 + 2 mg/L 2,4-D + 2 mg/L NAA + 20% coconut water + 1 mg/L of activated charcoal had induced high embryogenic frequent callus with length of 4–5 mm. The supplementation of 0.2 g/L colchicine and 100 μM 2,4-D was the most efficient in LS media. Over 70% of viable double haploid ELS were produced in 8 weeks and subcultured only twice compared with conventional anther which takes more than 12 weeks. This new technique can therefore be applied to rice in order in shorten time to produce higher number of double haploid plantlets.

scholarly journals Effect of Gibberellic Acid and Indole 3-Acetic Acid on Seed Germination Performance of Horse gram (Macrotyloma uniflorum) Lam (Verdc)

2016 ◽  
Vol 1 (2) ◽  
pp. 36 ◽  
Author(s):  
J. Lalitha ◽  
Haseena Rafath ◽  
M. Subash
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Potential Growth ◽  
Macrotyloma Uniflorum ◽  
Indole 3 Acetic Acid

The experiment was undertaken with an objective to investigate the effect of various concentrations of plant growth regulators, i.e., Gibberellic acid (GA3) and Indole 3-acetic acid (IAA) on seed germination of Macrotyloma uniflorum. Seeds were soaked for 12 hours in different concentrations substances (1.0, 2.0, 3.0 and 4.0 mg/L) of IAA, GA3 and control set was soaked only in distilled water.  Three replicates of each treatment with fifty seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (1 and 2 mg) of used hormones. Amongst the two potential growth regulators, 2 mg/L was found most effective because it showed highest germination percentage for IAA (93%) and GA3 (88%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient in overcoming dormancy leading to rapid seed germination. IAA was selected as best hormone in this study, which showed highest seed germination (93%). 

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