Using Two Retrotransposon Based Marker Systems (IRAP and REMAP) for Molecular Characterization of Olive (<i>Olea europaea</i> L.) Cultivars

Olive (Olea europaea L.) is one of the most characteristic agricultural trees of the Mediterranean region and has a large number of cultivar diversity. Olive cultivar characterization is very important especially for the fruit productivity and olive oil quality. In the present study, 46 clones belonging to Turkey (eight cultivars, each having five clones) and Italy (two cultivars, each having three clones) were assessed for cultivar characterization via inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) marker systems using 10 LTR and 10 ISSR primers. In total, 368 band profiles were obtained, 358 of which are polymorphic (97.28% polymorphism). The cultivars were segregated into three main groups, each group having several branches, where all the clones of each cultivar were belonging to the same main group. The only exception to that was the distribution of the clones of cultivar Yaglik, â€˜Yaglik 4â€™ and â€˜Yaglik 5â€™, into different main groups. IRAP and REMAP analysis showed a high level of genetic variability among the olive cultivars in this study and this marker systems would be useful tool for clonal selection programs.

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ISSR Analysis for Determination of Genetic Diversity and Relationship in Eight Turkish Olive (Olea europaea L.) Cultivars

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4319818 ◽

2015 ◽

Vol 43 (1) ◽

pp. 96-99 ◽

Cited By ~ 3

Author(s):

Ergun KAYA

Keyword(s):

Genetic Variability ◽

Clonal Selection ◽

Oil Quality ◽

Inter Simple Sequence Repeat ◽

Olive Cultivar ◽

Issr Analysis ◽

Main Cluster ◽

Fruit Productivity ◽

Olive Cultivars ◽

High Level

Olive cultivar identification is very important especially for fruit productivity and olive oil quality. Up to date, numerous techniques have been used for characterization of olive cultivars and detection of genetic variability. In the present study, 40 clones specific to Turkey (8 cultivars, each having 5 clones) were assessed for cultivar determination via inter simple sequence repeat (ISSR) marker systems using 10 ISSR primers. In total, 217 band profiles were obtained, 206 of which were polymorphic (94.9% polymorphism). The cultivars were segregated into two main clusters, each cluster being subdivided into two groups, while all the clones of a cultivar belong to the same main cluster. The only exception was the distribution of the clones of cultivar 'Tekir': 'Tekir 1', 'Tekir 2' and 'Tekir 3' on cluster III, while 'Tekir 4' and 'Tekir 5' were on cluster IV, therefore grouped into different main clusters. In the present study, ISSR analysis displayed a high level of genetic variability among Turkish olive cultivars, indicating a potential resource for the use of this germplasm in clonal selection programs.

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Characterization of Olive (Olea Europaea L.) Genetic Resources via PCR-Based Molecular Marker Systems

European Journal of Biology and Biotechnology ◽

10.24018/ejbio.2021.2.1.146 ◽

2021 ◽

Vol 2 (1) ◽

pp. 26-33

Author(s):

S. Galatali ◽

N. Abdul Ghafoor ◽

E. Kaya

Keyword(s):

Molecular Marker ◽

Genetic Resources ◽

Olea Europaea ◽

Oil Quality ◽

Mediterranean Area ◽

Olea Europaea L ◽

Olive Cultivars ◽

Olive Varieties ◽

Olea Europaéa

Olea europaea L., which is one of the ancient culture species cultivated in the Mediterranean area, has approximately 1200 cultivars. Its wild thype forms from O. europaea subsp. europaea var. sylvestris and its culture type forms from O. europaea subsp. europaea var. europaea. Olive cultivation is multiplied by grafting or cutting whereas wild type olive seedlings derived from seeds of O. europaea L. var. sylvestris. Because they have very big level heterozygosities, the genetic diversity of olive cultivars is quite high that they are predominantly allogamus. This genetic variability causes many confusions for identification of olive cultivars and since both oil quality and olive productivity are traits inherited to a variety, it is urgently needed to solve characterization and evaluation of olive genetic resources. The molecular marker systems are independent from environmental factors and effective technology to both detect homonymous and synonymous of cultivars and identify olive varieties. The current review is aimed to present molecular marker systems for identification and characterization of olive cultivars and emphasize their application for conservation of olive germplasm.

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CHANGES IN FRUIT YIELD AND PHOTOSYNTHESIS PARAMETERS IN DIFFERENT OLIVE CULTIVARS (Olea europaea L.) UNDER CON-TRASTING WATER REGIMES

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2020.3.12 ◽

2020 ◽

Vol 19 (3) ◽

pp. 135-147

Author(s):

Majid Golmohammadi ◽

Omid Sofalian ◽

Mehdi Taheri ◽

Alireza Ghanbari ◽

Valiollah Rasoli

Keyword(s):

Drought Stress ◽

Olea Europaea ◽

Principal Component ◽

Fruit Yield ◽

Photosynthetic Parameters ◽

Olive Cultivar ◽

Drought Treatment ◽

Olea Europaea L ◽

Olive Cultivars ◽

Olea Europaéa

The evergreen tree olive (Olea europaea L.) is the only species of the genus Olea that produces edible fruits with high ecological and economic value. This tree species has developed a series of physiochemical mechanisms to tolerate drought stress and grow under adverse climatic environments. One of these mechanisms is photosynthesis activities, so that as yet little information achieved about the relations between olive production and photosynthetic parameters under drought conditions. An experiment was carried out during two consecutive years (2015–2017) to study the response of 20 different olive tree cultivars (Olea europaea L.) to drought stress. Several parameters like net photosynthetic rate (PN), stomatal conductance (GS), transpiration rate (TE), photosynthetic pigments (total chlorophyll, chlorophyll a, b and carotenoid) and fruit yield were measured. The results of combined analysis of variance for fruit yield and other measured traits showed that year, drought treatment, cultivar main effects and their interactions were highly significant. The results indicated that drought stress reduced all traits, however GS (42.80%), PN (37.21%) and TE (37.17%) significantly affected by drought. Lower reduction in photosynthetic performance (PN, GS and TE) in the cultivar T7 compared to other olive cultivars allowed them to maintain better fruit yield. Principal component analysis (PCA) identified two PCs that accounted for 82.04 and 83.27% of the total variation in photosynthetic parameters under optimal and drought stress conditions, respectively. Taken together, mean comparison, relative changes due to drought and biplot analysis revealed that cultivars ‘T7’, ‘Roghani’, ‘Koroneiki’, ‘Korfolia’ and ‘Abou-satl’ displayed better response against drought stress. According to our results, one olive cultivar namely ‘T7’, could be used in olive breeding programs to improve new high yielding cultivars with drought tolerance for use in the drought-prone environments.

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Microsatellite markers for identification of a group of italian olive accessions

Scientia Agricola ◽

10.1590/s0103-90162009000500014 ◽

2009 ◽

Vol 66 (5) ◽

pp. 685-690 ◽

Cited By ~ 15

Author(s):

Innocenzo Muzzalupo ◽

Francesca Stefanizzi ◽

Amelia Salimonti ◽

Rosanna Falabella ◽

Enzo Perri

Keyword(s):

Microsatellite Markers ◽

Molecular Characterization ◽

Ssr Markers ◽

Olea Europaea ◽

Fruit Trees ◽

Ssr Primers ◽

Olive Cultivars ◽

Olea Europaéa

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.

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Achievements and Challenges towards a Sustainable Conservation and Use of ‘Galega vulgar’ Olea europaea Variety

Agronomy ◽

10.3390/agronomy10101467 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1467

Author(s):

Hélia Sales ◽

João Nunes ◽

Maria Carlota Vaz Patto

Keyword(s):

Olive Oil ◽

Olea Europaea ◽

Production Systems ◽

Oil Quality ◽

Olive Tree ◽

Life Cycle Thinking ◽

Mediterranean Countries ◽

Sustainable Conservation ◽

Olea Europaéa

The cultivated Olea europaea L., or olive tree, is an ancient crop extremely relevant for the bioeconomy of Mediterranean countries, especially for Portugal. With orchard modernization, Portugal has the potential to become the third-largest producer of olive oil over the next decade. In this country, the main national variety is ‘Galega vulgar,’ characterized by an excellent olive oil quality, rusticity and tolerance to drought. Nevertheless, its production has suffered a reduction due to replacement by foreign varieties. The present narrative review offers an overall perspective of present gaps and challenges to the conservation and use of ‘Galega vulgar.’ Existing information about traditional and innovative olive production systems, and the importance of life cycle thinking approaches for a sustainable olive chain were synthesized, focusing particularly in the last 10 years. Furthermore, the olive molecular characterization advances and their breeding importance were also evaluated considering their application to this Portuguese variety. To ensure an efficient and sustainable exploitation of the ‘Galega vulgar,’ we propose that efforts should concentrate on the characterization of the existing variability and development of genotype to phenotype prediction tools, integrating detailed molecular marker genotypic and environmental characterization, to support better informed conservation and breeding decisions in a multi-environment context.

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Floral Behavior, Fruit Characteristics and Oil Quality of Some Olive Cultivars "Olea europaea L."

Egyptian Journal of Horticulture ◽

10.21608/ejoh.2019.11082.1103 ◽

2019 ◽

Vol 46 (1) ◽

pp. 155-168

Author(s):

Zienab Ahmed ◽

Eman Taha ◽

Naglaa Abd-Elkarim

Keyword(s):

Olea Europaea ◽

Oil Quality ◽

Fruit Characteristics ◽

Olea Europaea L ◽

Olive Cultivars ◽

Olea Europaéa

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Microsatellite high-resolution melting (SSR-HRM) analysis for genotyping and molecular characterization of an Olea europaea germplasm collection

Plant Genetic Resources ◽

10.1017/s147926211400001x ◽

2014 ◽

Vol 12 (3) ◽

pp. 273-277 ◽

Cited By ~ 24

Author(s):

Aliki Xanthopoulou ◽

Ioannis Ganopoulos ◽

Georgios Koubouris ◽

Athanasios Tsaftaris ◽

Chrysa Sergendani ◽

...

Keyword(s):

High Resolution ◽

Microsatellite Markers ◽

Molecular Characterization ◽

Olea Europaea ◽

High Resolution Melting ◽

Germplasm Collection ◽

Hrm Analysis ◽

Olive Cultivars ◽

Olea Europaéa

Olea europaea L. has been cultivated in the Mediterranean region for thousands of years and is of major economic importance. The origin of olive cultivars remains as complex to trace as their identification. Thus, their molecular characterization and discrimination will enable olive germplasm management. In addition, it would be a useful tool for authentication of olive products. High-resolution melting (HRM) analysis, coupled with five microsatellite markers, was integrated to facilitate molecular identification and characterization of main O. europaea cultivars collected from the National Olive Tree Germplasm Collection established in Chania, Greece. The five microsatellite loci used were highly informative and generated a unique melting curve profile for each of the 47 cultivars and for each microsatellite tested. In particular, three microsatellite markers (DCA03, DCA09 and DCA17), which generated 29 HRM profiles, were sufficient to genotype all the olive cultivars studied, highlighting their potential use for cultivar identification. Furthermore, this assay provided a flexible, cost-effective and closed-tube microsatellite genotyping method well suited for molecular characterization of olive cultivars.

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