scholarly journals Mycorrhiza and Common Mycorrhizal Network Regulate the Production of Signal Substances in Trifoliate Orange (Poncirus trifoliata)

2017 ◽  
Vol 45 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Yi-Can ZHANG ◽  
Chun-Yan LIU ◽  
Qiang-Sheng WU
Keyword(s):  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Synthetic Genes ◽  
Nylon Mesh ◽  
Mycorrhizal Network ◽  
Mycorrhizal Networks ◽  
Regulated Expression ◽  
Promote Plant Growth ◽  
Donor Chamber ◽  
Amf Inoculation

Common mycorrhizal networks (CMNs) connecting two or more neighbouring plants are confirmed to transfer signals, whereas little information about CMNs effects on the signal substances production is known. In this study, a two-chambered rootbox separated by 37 µm nylon mesh was used to establish donor and receptor chambers. Two chambers both were planted with trifoliate orange (Poncirus trifoliata) and then only donor chamber inoculated with Diversispora versiformis, Paraglomus occultum and Rhizoglomus intraradices. The roots of the donor and receptor plants both were mycorrhizated suggesting that CMNs were established between donor and receptor seedlings. Moreover, the AMF association dramatically increased plant height, stem diameter, leaf numbers, and shoot and root biomass in both the donor and receptor seedlings. The AMF inoculation in the donor plants and the subsequent mycorrhizal colonization by CMNs in the receptor plants significantly increased root calmodulin (CaM) and salicylic acid (SA) concentrations, while considerably decreased root nitric oxide (NO) and jasmonic acid (JA) concentrations. This was accompanied by down-regulated expression of three JA synthetic genes (PtLOX, PtAOS and PtAOC), regardless of donor and receptor seedlings. These results thus suggest that CMNs between trifoliate orange seedlings manifestly promote plant growth and affect the production of signal substances.

scholarly journals Exogenous Phytohormones Modulate Mycorrhiza-Induced Changes in Root Hair Configuration of Trifoliate Orange

2016 ◽  
Vol 44 (2) ◽  
pp. 548-556 ◽  
Author(s):  
Chun-Yan LIU ◽  
A.K. SRIVASTAVA ◽  
De-Jian ZHANG ◽  
Ying-Ning ZOU ◽  
Qiang-Sheng Wu
Keyword(s):  
Root Hair ◽  
Lateral Roots ◽  
Poncirus Trifoliata ◽  
Hair Length ◽  
Trifoliate Orange ◽  
Hair Density ◽  
Root Hair Length ◽  
Nylon Mesh ◽  
Root Hair Density ◽  
Induced Changes

Mycorrhizas alter root hair profile, but it is not clear whether exogenous phytohormones regulate the mycorrhizal effects on root hair. Studies were carried out in a two-chambered rootbox separated by 37-μm nylon mesh to establish root+hyphae chamber carrying trifoliate orange [Poncirus trifoliata (L.) Raf.] as the test plant inoculated with Diversispora versiformis and hyphae chamber (without roots). Indole butyric acid (IBA), abscisic acid (ABA), and jasmonic acid (JA) (each at 0.1 μM concentration) were weekly applied into hyphae chamber, in total of six times before plant harvest. Mycorrhization strongly stimulated plant growth performance, and exogenous phytohormones, especially IBA, further magnified the mycorrhizal-stimulated growth responses. Three exogenous phytohormones decreased mycorrhizal colonization in taproot and first-order lateral roots, but increased in second- and third-order lateral roots. These phytohormones also increased hyphal length in nylon mesh and soil, irrespective of root+hyphae or hyphae chamber. Mycorrhizal inoculation significantly increased root hair density of different root classes, and exogenous hormones further strengthened the mycorrhizal effect. Average root hair length was stimulated by mycorrhization, but all exogenous phytohormones weakened the mycorrhizal response. Mycorrhization in combination with exogenous phytohormones showed no response on root hair diameter. Our studies, hence, suggested that application of exogenous phytohormones in hyphae chamber strengthened the D. versiformis-induced changes in average root hair density but weakened in average root hair length in trifofliate orange grown in root+hyphae chamber.

scholarly journals Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

2010 ◽  
Vol 30 (3) ◽  
pp. 431-439 ◽  
Author(s):  
F. Nishikawa ◽  
T. Endo ◽  
T. Shimada ◽  
H. Fujii ◽  
T. Shimizu ◽  
...  
Keyword(s):  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Transcriptional Changes

scholarly journals Effects of Common Mycorrhizal Network on Plant Carbohydrates and Soil Properties in Trifoliate Orange–White Clover Association

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142371 ◽  
Author(s):  
Ze-Zhi Zhang ◽  
You-Gen Lou ◽  
Dao-Juan Deng ◽  
Mohammed Mahabubur Rahman ◽  
Qiang-Sheng Wu
Keyword(s):  
Soil Properties ◽  
White Clover ◽  
Trifoliate Orange ◽  
Mycorrhizal Network ◽  
Common Mycorrhizal Network

scholarly journals Community Analysis of Arbuscular Mycorrhizal Fungi in Roots ofPoncirus trifoliataandCitrus reticulataBased on SSU rDNA

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Wang ◽  
Yin Wang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Small Subunit ◽  
Poncirus Trifoliata ◽  
Spore Density ◽  
Morphological Observation ◽  
Trifoliate Orange ◽  
Hyphal Length

Morphological observation of arbuscular mycorrhizal fungi (AMF) species in rhizospheric soil could not accurately reflect the actual AMF colonizing status in roots, while molecular identification of indigenous AMF colonizing citrus rootstocks at present was rare in China. In our study, community of AMF colonizing trifoliate orange (Poncirus trifoliataL. Raf.) and red tangerine (Citrus reticulataBlanco) were analyzed based on small subunit of ribosomal DNA genes. Morphological observation showed that arbuscular mycorrhizal (AM) colonization, spore density, and hyphal length did not differ significantly between two rootstocks. Phylogenetic analysis showed that 173 screened AMF sequences clustered in at least 10 discrete groups (GLO1~GLO10), all belonging to the genus ofGlomusSensu Lato. Among them, GLO1 clade (clustering with uncultured Glomus) accounting for 54.43% clones was the most common in trifoliate orange roots, while GLO6 clade (clustering withGlomus intraradices) accounting for 35.00% clones was the most common in red tangerine roots. Although, Shannon-Wiener indices exhibited no notable differences between both rootstocks, relative proportions of observed clades analysis revealed that composition of AMF communities colonizing two rootstocks varied severely. The results indicated that native AMF species in citrus rhizosphere had diverse colonization potential between two different rootstocks in the present orchards.

scholarly journals ERF109 of trifoliate orange (Poncirus trifoliata(L.) Raf.) contributes to cold tolerance by directly regulating expression ofPrx1involved in antioxidative process

2019 ◽  
Vol 17 (7) ◽  
pp. 1316-1332 ◽  
Author(s):  
Min Wang ◽  
Wenshan Dai ◽  
Juan Du ◽  
Ruhong Ming ◽  
Bachar Dahro ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange
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