scholarly journals Roles of Some Plant Growth Regulators on Natural and Dark-Induced Senescence in Wheat Leaf Segments

2019 ◽  
Vol 47 (4) ◽  
pp. 1230-1237
Author(s):  
Nihal GÖREN-SAĞLAM ◽  
Fazilet Özlem ÇEKIÇ
Keyword(s):  
Cell Death ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Digital Object Identifier ◽  
Light Conditions ◽  
Wheat Leaf ◽  
Gaba Content ◽  
Leaf Segments ◽  
Dark Conditions

Senescence is an important developmental process in the life span of a plant. It is affected by several endogenous and exogenous factors such as hormones, darkness, and drought. Hormones are major endogenous factors that affect senescence intensively. In this study, our aim was to investigate the effects of different plant growth regulators; benzyl adenin (BA), spermine (Spm) and spermidine (Spd) on both natural and dark-induced senescence and on γ-aminobutyric acid (GABA) content in wheat leaf segments. Following the incubation, fresh and dry weight, chlorophyll and carotenoid contents; peroxidase activity, soluble protein, GABA and malondialdehyde (MDA) contents and cell death amount were determined. Our results demonstrate that Spm and Spd accelerated senescence under light and delayed it under dark conditions. BA has delayed leaf senescence under both conditions. Cell death and MDA content showed a correlation with the senescence process. GABA content was enhanced considerably by BA, Spm and Spd applications under light conditions. According to our results we can conclude that Spm and Spd could promote senescence on wheat segments under light conditions, whereas inhibit senescence under dark conditions. In addition, BA could retard the deleterious effects on senescence under both light and dark conditions.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

scholarly journals Investigation of callogenesis and indirect regeneration of Freesia × hybrida Bailey ‘Argenta’

2015 ◽  
Vol 27 (2) ◽  
pp. 123-134 ◽  
Author(s):  
Ali Pourkhaloee ◽  
Morteza Khosh-Khui
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cold Storage ◽  
Embryogenic Callus ◽  
Naphthaleneacetic Acid ◽  
Light Conditions ◽  
High Quality ◽  
Freesia Hybrida ◽  
Different Types

AbstractAn investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (Freesia×hybridaBailey ‘Argenta’). Sections of two different types of explants, corms and pupae (cold storage-produced corms), were placed on Murashige and Skoog (MS) media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%), the highest callus growth (15 mm diameter) and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−11-naphthaleneacetic acid (NAA) and 2 mg L−16-benzylaminopurine (BAP) in the dark. Increasing BAP up to 3 to 4.5 mg L−1resulted in the maximum number of regenerated cormlets from 1 cm2calli (2 cormlets) under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1indole-3-butyric acid (IBA). In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1). The results indicated that 15 g L−1maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1BAP and 1 mg L−1NAA.

scholarly journals Response of Hybrid Cymbidium (Orchidaceae) Protocorm-Like Bodies to 26 Plant Growth Regulators

2014 ◽  
Vol 20 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Jaime A. Teixeira Da Silva
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Effective Means ◽  
Multiple Shoots ◽  
Growth Inhibitors ◽  
Light Conditions ◽  
Cell Layers ◽  
Organogenesis In Vitro

Abstract Teixeira da Silva J.A., 2014: Response of hybrid Cymbidium (Orchidaceae) protocorm-like bodies to 26 plant growth regulators [Cymbidium (Orchidaceae) hibrido į protokormą panašių kūnelių reakcija į 26 augalų augimo reguliatorius]. - Bot. Lith., 20(1): 3-13. Plant growth regulators (PGRs) are one of the most effective means of controlling plant organogenesis in vitro. Hybrid orchid production relies on effective protocols to maximize clonal shoot output. This is best achieved when protocormlike bodies (PLBs) are propagated. In a bid to deepen orchidologists’ understanding of basic responses of Cymbidium to PGRs, this study aimed to establish the organogenic response of hybrid Cymbidium Twilight Moon ‘Day Light’ half-PLBs or PLB thin cell layers (TCLs) to a single application of PGRs (6 auxins; 7 cytokinins; 3 alternative PGRs), 3 herbicides or 7 growth inhibitors/retardants at 4 concentrations (1, 2, 4 or 8 mg·1-1) as well as a control (0 mg·1-1), both in the light and in the dark. The control (PGR-containing TC medium) performed best, but all auxins and growth inhibitors and retardants were toxic to neo-PLB formation, resulting in 100% death. A synthetic auxin (BSAA), a cytokinin (4-CPPU) and two herbicides (dicamba and picloram) were equally toxic. No auxins, TIBA, GA3 or SA induced any organogenic response. 1 or 2 mg·1-1 2,4-D or 1 mg·1-1 TDZ induced embryogenic callus, but 2-8 mg·1-1 2,4-D resulted in abnormal shoots. TDZ induced direct multiple shoots. Only five remaining cytokinins (Ads, BA, Kin, ZR, 2iP) could form neo-PLBs, but always significantly less than the controls, independent of the explant used (half- PLBs or tTCLs) and light conditions (light vs darkness). These five cytokinins could be useful for neo-PLB induction of other Cymbidium hybrids. A new concept, the average cumulative value or ACV, is introduced

Wheat regenerants in the system of rapid assessment of the effect of 24-epibrassinolide

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 394-397
Author(s):  
Seldimirova O.A. ◽  
M.V. Bezrukova ◽  
N.N. Кruglova ◽  
F.М. Shakirova
Keyword(s):  
Plant Growth ◽  
Experimental Model ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Drought Resistance ◽  
Rapid Assessment ◽  
Immature Embryo ◽  
Model System ◽  
Wheat Cultivars ◽  
Experimental Model System

The influence of 24-epibrassinolide on the efficiency of regenerants obtained from embryonic calli formation was studied in wheat cultivars contrast for drought resistance. The possibility of using the experimental model system «immature embryo – embryonic callus – regenerant» in the rapid assessment of the effect of antistress plant growth regulators is shown.

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