scholarly journals Use of antioxidants and time of cold storage: effects over viability parameters and enzymatic levels in semen of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792).

2023 ◽  
Vol 83 ◽  
Author(s):  
E. Ibáñez-Arancibia ◽  
J. G. Farías ◽  
I. Valdebenito
Keyword(s):  
Free Radicals ◽  
Enzymatic Activity ◽  
Seminal Plasma ◽  
Cold Storage ◽  
Membrane Lipids ◽  
Main Process ◽  
Antioxidant Defences ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Storage Effects ◽  
Capacity Disruptions

Abstract The cold storage of milt implies potentials alterations in its quality because the storage generates as main process, free radicals that produce spermatozoa membrane lipids damage with the consequent motility and fertilising capacity disruptions. To decrease the damage generated by free radicals the cells have antioxidant defences (proteins, enzymes, and low molecular weight substances). The objective of the present study evaluated the time storage effect and different antioxidants prepared in spermatic diluents on sperm viability of O. mykiss milt stored at 4°C. The two-way ANOVA denoted that the time storage and antioxidant influence have significant effects separated or combined on viability parameters (sperm motility and viability, proteins concentrations and superoxide dismutase enzymatic activity in seminal plasma). In contrast, only the storage time affected the fertilising capacity and catalase enzymatic activity in seminal plasma. The resulting analysis can conclude that the antioxidant presence improves the viability of cold stored milt, especially the transport conditions and the antioxidants allow the fecundity despite motility decrease.

Comparison of Different Peptidase Substrates for Evaluation of Microbial Quality of Aerobically Stored Meats

2000 ◽  
Vol 63 (10) ◽  
pp. 1447-1449 ◽  
Author(s):  
L. STEPANIAK
Keyword(s):  
Enzymatic Activity ◽  
Correlation Coefficient ◽  
Cold Storage ◽  
Microbial Quality ◽  
Microbial Cells ◽  
Clear Advantage ◽  
Yellow Color ◽  
Meat Samples

Different aminopeptidase and endopeptidase substrates were assessed for the detection of enzymatic activity of microorganisms collected from the surface of aerobically cold-stored pork and beef. The most sensitive substrates were fluorogenic Ala-7-amino-4-methylcoumarin (Ala-AMC) or Leu-AMC and colorogenic Ala-p-nitroanilide (Ala-pNA). Activity on natural oligopeptides, e.g., bradykinin or αs1 casein fragment 1 to 23, was very low. The correlation coefficient (r) between log surface counts of 66 meat samples and log fluorescence or absorbance after incubation of surface microbial cells for 2 h with Ala-AMC, Leu-AMC, and Ala-pNA was 0.89, 0.83, and 0.82, respectively. A distinct yellow color was obtained with Ala-pNA when the surface count was ∼106 CFU/cm2. Although correlation and sensitivity was better, no clear advantage is obtained with the use of the fluorogenic Ala-AMC or Leu-AMC instead of Ala-pNA, a substrate proposed by Alvarado et al. (J. Food Sci. 57:1330, 1992) for rapidly assessing the microbial quality of refrigerated meat. The correlation coefficient (r) between time of cold storage and surface count was 0.69.

Apple fruit texture QTLs: year and cold storage effects on sensory and instrumental traits

2015 ◽  
Vol 11 (6) ◽  
Author(s):  
Inès Ben Sadok ◽  
Aline Tiecher ◽  
Didiana Galvez-Lopez ◽  
Marc Lahaye ◽  
Pauline Lasserre-Zuber ◽  
...  
Keyword(s):  
Cold Storage ◽  
Apple Fruit ◽  
Fruit Texture ◽  
Storage Effects

The Role of Free Radicals and Membrane Lipids in Diabetes-Induced Congenital Malformations

1998 ◽  
Vol 5 (4) ◽  
pp. 178-187 ◽  
Author(s):  
E. Albert Reece ◽  
Carol J. Homko ◽  
Ying-King Wu ◽  
Arnon Wiznitzer
Keyword(s):  
Free Radicals ◽  
Congenital Malformations ◽  
Membrane Lipids

Free Radicals in Down's Syndrome, Amyotrophic lateral sclerosis & Rheumatoid Arthritis

2017 ◽  
Author(s):  
Luke Sammut
Keyword(s):  
Rheumatoid Arthritis ◽  
Amyotrophic Lateral Sclerosis ◽  
Free Radicals ◽  
Free Radical ◽  
Transition Metals ◽  
Tissue Injury ◽  
Down's Syndrome ◽  
Down’S Syndrome ◽  
Antioxidant Defences ◽  
Lateral Sclerosis

A free radical is an atom or group of atoms that have one or more unpaired electrons that can be considered as fragments of molecules and which are generally very reactive. Free Radicals can have positive, negative or neutral charge. They are produced continuously in cells either as accidental by-products of metabolism or deliberately during, for example, phagocytosis. The most important reactants in free radical biochemistry in aerobic cells are oxygen and its radical derivatives (superoxide and hydroxyl radical), hydrogen peroxide and transition metals. Cells have developed a comprehensive array of antioxidant defences to prevent free radical formation or limit their damaging effects. These include enzymes to decompose peroxides, proteins to sequester transition metals and a range of compounds to ‘scavenge’ free radicals. Reactive free radicals formed within cells can oxidise biomolecules and lead to cell death and tissue injury. Establishing the involvement of free radicals in the pathogenesis of a disease is extremely difficult due to the short lifetimes of these species. We describe the role of free radicals in Down's syndrome, Amyotrophic lateral sclerosis and Rheumatoid arthritis.

Sign in / Sign up

Export Citation Format

Share Document