scholarly journals Combining disease resistance and postharvest quality traits by early marker-assisted backcrossing in carioca beans

2022 ◽  
Vol 79 (2) ◽  
Author(s):  
Jean Fausto de Carvalho Paulino ◽  
Caléo Panhoca de Almeida ◽  
Isabella Laporte Santos ◽  
João Guilherme Ribeiro Gonçalves ◽  
Sérgio Augusto Morais Carbonell ◽  
...  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Penghua Gao ◽  
Hao Zhang ◽  
Huijun Yan ◽  
Qigang Wang ◽  
Bo Yan ◽  
...  

Abstract Background Rose is an important economic crop in horticulture. However, its field growth and postharvest quality are negatively affected by grey mould disease caused by Botrytis c. However, it is unclear how rose plants defend themselves against this fungal pathogen. Here, we used transcriptomic, metabolomic and VIGS analyses to explore the mechanism of resistance to Botrytis c. Result In this study, a protein activity analysis revealed a significant increase in defence enzyme activities in infected plants. RNA-Seq of plants infected for 0 h, 36 h, 60 h and 72 h produced a total of 54 GB of clean reads. Among these reads, 3990, 5995 and 8683 differentially expressed genes (DEGs) were found in CK vs. T36, CK vs. T60 and CK vs. T72, respectively. Gene annotation and cluster analysis of the DEGs revealed a variety of defence responses to Botrytis c. infection, including resistance (R) proteins, MAPK cascade reactions, plant hormone signal transduction pathways, plant-pathogen interaction pathways, Ca2+ and disease resistance-related genes. qPCR verification showed the reliability of the transcriptome data. The PTRV2-RcTGA1-infected plant material showed improved susceptibility of rose to Botrytis c. A total of 635 metabolites were detected in all samples, which could be divided into 29 groups. Metabonomic data showed that a total of 59, 78 and 74 DEMs were obtained for T36, T60 and T72 (T36: Botrytis c. inoculated rose flowers at 36 h; T60: Botrytis c. inoculated rose flowers at 60 h; T72: Botrytis c. inoculated rose flowers at 72 h) compared to CK, respectively. A variety of secondary metabolites are related to biological disease resistance, including tannins, amino acids and derivatives, and alkaloids, among others; they were significantly increased and enriched in phenylpropanoid biosynthesis, glucosinolates and other disease resistance pathways. This study provides a theoretical basis for breeding new cultivars that are resistant to Botrytis c. Conclusion Fifty-four GB of clean reads were generated through RNA-Seq. R proteins, ROS signalling, Ca2+ signalling, MAPK signalling, and SA signalling were activated in the Old Blush response to Botrytis c. RcTGA1 positively regulates rose resistance to Botrytis c. A total of 635 metabolites were detected in all samples. DEMs were enriched in phenylpropanoid biosynthesis, glucosinolates and other disease resistance pathways.


2021 ◽  
Vol 43 (5) ◽  
Author(s):  
João Claudio Vilvert ◽  
Sérgio Tonetto de Freitas ◽  
Maria Aparecida Rodrigues Ferreira ◽  
Eleonora Barbosa Santiago da Costa ◽  
Edna Maria Mendes Aroucha

Abstract The objective of this study was to determine the most efficient sample size required to estimate the mean of postharvest quality traits of ‘Palmer’ mangoes harvested in two growing seasons. A total of 50 mangoes were harvested at maturity stage 2, in winter (June 2020) and spring (October 2020), and evaluated for weight, length, ventral and transverse diameter, skin and pulp L*, C* and hº, dry matter, firmness, soluble solids (SS), titratable acidity (TA) and the SS/TA ratio. According to the results, the coefficient of variation (CV) of fruit quality traits ranged from 2.1% to 18.1%. The highest CV in both harvests was observed for the SS/TA ratio, while the lowest was reported for pulp hº. In order to estimate the mean of physicochemical traits of ‘Palmer’ mangoes, 12 fruits are needed in the winter and 14 in the spring, considering an estimation error of 10% and a confidence interval of 95%. TA and the SS/TA ratio required the highest sample size, while L* and hº required the lowest sample size. In conclusion, the variability was different among physicochemical traits and seasons, implying that different sample sizes are required to estimate the mean of different quality traits in different growing seasons.


2007 ◽  
pp. 309-317
Author(s):  
J. Obando ◽  
J.A. Martínez ◽  
M.J. Roca ◽  
A. Alarcón ◽  
J.P. Fernández-Trujillo ◽  
...  

Genome ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 644-656 ◽  
Author(s):  
Inge Van Daele ◽  
Hilde Muylle ◽  
Erik Van Bockstaele ◽  
Isabel Roldán-Ruiz

Several linkage maps, mainly based on anonymous markers, are now available for Lolium perenne . The saturation of these maps with markers derived from expressed sequences would provide information useful for QTL mapping and map alignment. Therefore, we initiated a study to develop and map DNA markers in genes related to self-incompatibility, disease resistance, and quality traits such as digestibility and sugar content in two L. perenne families. In total, 483 and 504 primer pairs were designed and used to screen the ILGI and CLO-DvP mapping populations, respectively, for length polymorphisms. Finally, we were able to map 67 EST markers in at least one mapping population. Several of these markers coincide with previously reported QTL regions for the traits considered or are located in the neighbourhood of the self-incompatibility loci, S and Z. The markers developed expand the set of gene-derived markers available for genetic mapping in ryegrasses.


Crop Science ◽  
1999 ◽  
Vol 39 (5) ◽  
pp. 1271-1276 ◽  
Author(s):  
C.E.L. Fonseca ◽  
D.R. Viands ◽  
J.L. Hansen ◽  
A.N. Pell

2018 ◽  
Vol 137 (6) ◽  
pp. 883-894
Author(s):  
Sandip K. Bera ◽  
Surendra S. Manohar ◽  
Murali T. Variath ◽  
Sunil Chaudhari ◽  
Shasidhar Yaduru ◽  
...  

2017 ◽  
Vol 8 (2) ◽  
pp. 485
Author(s):  
Sunil Chaudhari ◽  
D. Khare ◽  
S. Sundravadana ◽  
Murali T. Variath ◽  
Surendra S. Manohar ◽  
...  

HortScience ◽  
2017 ◽  
Vol 52 (6) ◽  
pp. 880-886 ◽  
Author(s):  
Cristina Pisani ◽  
Mark A. Ritenour ◽  
Ed Stover ◽  
Anne Plotto ◽  
Rocco Alessandro ◽  
...  

Avocado (Persea americana Mill.) is a high-value fruit that continues to increase in consumer demand. A population of ‘Hass’–‘Bacon’ hybrids was planted at USDA-ARS, Fort Pierce, as part of a study to find selections with good horticultural and postharvest quality traits for Florida. Extensive phenotypic data on quality were collected over 3 years. Ten selections were identified in 2014 and 2015 with promising fruit quality and postharvest shelf life characteristics and were tested in sensory panels using store-bought ‘Hass’ as the standard. In general, the selections had fruit quality similar to commercial ‘Hass’. Avocados that were most liked were described as creamy in texture with buttery and nutty flavor. Only one selection (R7T54 in 2014) and one store-bought control (‘Hass’ in 2015) were disliked, which was associated with greater firmness at the time of evaluation, likely relating to insufficient postharvest conditioning. Furthermore, CA ‘Hass’ commercial requirements for minimum dry matter (20.8%) were generally achieved by these selections under Florida conditions, ranging from 18.4% to 25.7%. This study identified 10 selections with composition and sensory quality similar to ‘Hass’ that are suitable for further testing and development in Florida.


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