scholarly journals LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

2018 ◽  
Vol 42 (6) ◽  
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
Natane Amaral Miranda ◽  
Joane Helena Maggioni
Keyword(s):  
Light Source ◽  
Culture Medium ◽  
Light Quality ◽  
Shoot Length ◽  
Nodal Segments ◽  
Fluorescent Lamp ◽  
Corymbia Citriodora ◽  
Hybrid Clones ◽  
Effect Of Light

ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.

scholarly journals In vitro establishment of pepper-rosmarin nodal segments

2008 ◽  
Vol 26 (2) ◽  
pp. 255-258 ◽  
Author(s):  
Arie F Blank ◽  
Andréa S da Costa ◽  
Maria de Fátima Arrigoni-Blank ◽  
Aline B de Mendonça ◽  
Ana da S Ledo
Keyword(s):  
Essential Oil ◽  
Medicinal Plant ◽  
Growth Regulators ◽  
Culture Medium ◽  
Antimicrobial Properties ◽  
Shoot Length ◽  
Nodal Segments ◽  
Lippia Sidoides ◽  
In Vitro Establishment

Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA (0.0; 0.5; 1.0; 1.5; and 2.0 mg L-1) in assay 4; and five concentrations of GA3 (0.0; 0.1; 0.5; 1.0; and 1.5 mg L-1) in assay 5. The use of BAP, IBA, NAA and KIN did not influence the number of shoots. The addition of 0.1 mg L-1 of NAA at the culture medium resulted in larger shoot length per explant, while the concentration of 1.0 mg L-1 of kinetin promoted larger shoot length in general. GA3 did not affect the elongation of pepper-rosmarin shoots when cultivated in vitro.

scholarly journals Multiplicação in vitro de Caesalpinia pyramidalis (Leguminosae)

2013 ◽  
Vol 13 ◽  
Author(s):  
Tecla Dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Bárbara Paula dos Santos Borges ◽  
Bruno Freitas Matos Alvim ◽  
José Raniere Ferreira De Santana
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Dry Mass ◽  
Shoot Length ◽  
Nodal Segments ◽  
In Vitro Multiplication ◽  
Different Types

Caesalpinia pyramidalis is a species endemic to the Caatinga and known popularly as catingueira, which is widely used by local people, mainly for its timber and medicinal and fodder properties. This study investigated the effects of different types and concentrations of plant growth regulators on the in vitro multiplication of C. pyramidalis. In the first experiment, nodal segments were inoculated in media containing different combinations (0.0–8.0 µM) of BAP and NAA. In the second experiment, nodal segments wereinoculated in media containing different types (KIN, BAP and TDZ) and concentrations (0.0–16μM) of cytokinins. We used a WPM medium supplemented with 87.64 mM sucrose and solidified with 7.0 g L-1 agar. After 45 days, the highest number of shoots, leaf number, shoot length and dry mass of shoots were obtained when nodal segments were inoculated into a culture medium without plant growth regulators.

scholarly journals ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

2017 ◽  
Vol 41 (1) ◽  
Author(s):  
Leandro Silva Oliveira ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
José Marcello Salabert Campos ◽  
Lyderson Facio Viccini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microsatellite Markers ◽  
Genetic Stability ◽  
Culture Medium ◽  
Eucalyptus Grandis ◽  
Genetic Fidelity ◽  
Shoot Multiplication ◽  
Micropropagated Plants ◽  
Hybrid Clones

ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation was performed in MS culture medium supplemented with AIB (2.46 µM) and BA(0.22 µM). The ex vitro rooting and acclimatization phases were lead at the same time. The micropropagated clones showed genetic stability by flow cytometry and microsatellite markers. The results proved that micropropagation, for purposes of rejuvenation, can be a viable technique to generate genetically stable or identical E. globulus hybrid clones.

scholarly journals Types and concentrations of cytokinins in the micropropagation of Anthurium maricense

2018 ◽  
Vol 12 (2) ◽  
pp. 117
Author(s):  
Cecília Moreira Serafim ◽  
Arlene Santisteban Campos ◽  
Priscila Bezerra Dos Santos Melo ◽  
Ana Cecília Ribeiro de Castro ◽  
Ana Cristina Portugal Pinto de Carvalho
Keyword(s):  
Light Intensity ◽  
Culture Medium ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Viable Option ◽  
Growth Room ◽  
In Vitro Induction

Faced with the demand for plants potted for their foliage, Anthurium maricense is seen as a viable option. However, most of the studies on obtaining micropropagated plantlets are for A. andraeanum, with nothing yet reported for A. maricense. The aim of this study therefore, was to evaluate the effect of four cytokinins in different concentrations, on the in vitro induction of shoots from nodal segments of A. maricense. The experimental design was completely randomised in a 4 x 4 factorial scheme, with four cytokinins (BAP, ZEA, CIN and 2iP) and 4 concentrations (0, 2.22, 4.44 and 6.66 μM), for a total of 16 treatments, with 6 replications of five test tubes, and using one nodal segment. Cultures were kept in a growth room at 25 ± 2°C, a photoperiod of 16 h and a light intensity of 30 μmolm-2 s-1 for 60 days. After this period, the number of shoots formed per node was evaluated. The addition of a cytokinin to the culture medium was determinant for the in vitro regeneration of shoots in A. maricense. The greatest estimated number of shoot formations in A. maricense were obtained in the culture media containing ZEA (3.87) and BAP (3.30), both at concentration of 6.66 μM.

Biotechnological methods of in vitro propagation in willows (Salix spp.)

2012 ◽  
Vol 7 (5) ◽  
pp. 931-940 ◽  
Author(s):  
Dagmar Skálová ◽  
Božena Navrátilová ◽  
Lenka Richterová ◽  
Michal Knit ◽  
Michal Sochor ◽  
...  
Keyword(s):  
Central Europe ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Reproductive Potential ◽  
Ovule Culture ◽  
Nodal Segments ◽  
Young Shoot ◽  
Fragmented Populations ◽  
Salix Spp

AbstractMany populations of high-mountainous relic dioecious willows in Central Europe only consist of female individuals and are thus limited in their reproductive potential. We completed micropropagation experiments with shoot apexes and nodal segments of common and endangered willow (Salix) species, which can help to reintroduce autochthonous genotypes to their natural sites. Until recently, cultivation of green young shoot apexes of S. alba and S. lapponum showed the highest percentage of regeneration. We successfully applied the two-times-sterilisation due to high contamination of natural explants. The OK medium was the most efficient culture medium. In vitro propagation of willows with unisexual catkins, anther and ovule cultures were tested and optimised. Isolated anthers were cultivated on selected media and then microcallus and calluses of S. caprea and calluses of S. viminalis were formed on the A medium. Among various tested and optimised media for the ovule culture, the CP medium was the most efficient one. In this case, only the microcalluses of S. viminalis were observed. We developed biotechnological procedures that can be useful in conserving fragmented populations of high-mountainous willows.

The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis ‘Spring Dancer’

2018 ◽  
Vol 59 (2) ◽  
pp. 179-188 ◽  
Author(s):  
A-Reum Kwon ◽  
Myung-Min Oh ◽  
Kee-Yoeup Paek ◽  
So-Young Park
Keyword(s):  
Light Quality ◽  
Effect Of Light

Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones

1994 ◽  
Vol 13 (3) ◽  
pp. 143-146 ◽  
Author(s):  
Nina P. Aksenova ◽  
Tatyana N. Konstantinova ◽  
Lydiya I. Sergeeva ◽  
Ivana Macháčková ◽  
Svetlana A. Golyanovskaya
Keyword(s):  
Light Quality ◽  
Potato Plants ◽  
Potato Plants In Vitro ◽  
Effect Of Light

scholarly journals Micropropagation of Isoplexis chalcantha Svent, O´Shanahan from Mature Plants

1970 ◽  
Vol 18 (2) ◽  
pp. 131-137 ◽  
Author(s):  
S. Mederos-Molina
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Nodal Segments ◽  
Axillary Shoots ◽  
In Vitro Shoots ◽  
Half Strength ◽  
Modified Ms Medium ◽  
High Degree ◽  
First Time

Culture medium requirements for micropropagation of Isoplexis chalcantha was achieved for the first time after high degree of contamination and phenolic exudates were detected and solved. Cultures were established from axillary shoots using juvenile branches collected from this medicinal plant. Most satisfying results were obtained using a solidified and a modified MS medium (NO3- : NH4+ ratios) enriched with ascorbic acid or soluble PVP plus GA3, BAP and NAA. Explants (nodal segments) were used for in vitro shoots multiplication and best results were achieved with modified MS plus BAP and auxins. Vigorous shoots rooted without symptoms in the half-strength modified MS enriched with low concentration of IBA. Key words: Isoplexis chalcantha, axillary shoots, contamination, phenolic exudates, culture media, NO3- : NH4+ ratios D.O.I. 10.3329/ptcb.v18i2.3395 Plant Tissue Cult. & Biotech. 18(2): 131-137, 2008 (December)

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