scholarly journals In vitro establishment of pepper-rosmarin nodal segments

2008 ◽  
Vol 26 (2) ◽  
pp. 255-258 ◽  
Author(s):  
Arie F Blank ◽  
Andréa S da Costa ◽  
Maria de Fátima Arrigoni-Blank ◽  
Aline B de Mendonça ◽  
Ana da S Ledo
Keyword(s):  
Essential Oil ◽  
Medicinal Plant ◽  
Growth Regulators ◽  
Culture Medium ◽  
Antimicrobial Properties ◽  
Shoot Length ◽  
Nodal Segments ◽  
Lippia Sidoides ◽  
In Vitro Establishment

Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA (0.0; 0.5; 1.0; 1.5; and 2.0 mg L-1) in assay 4; and five concentrations of GA3 (0.0; 0.1; 0.5; 1.0; and 1.5 mg L-1) in assay 5. The use of BAP, IBA, NAA and KIN did not influence the number of shoots. The addition of 0.1 mg L-1 of NAA at the culture medium resulted in larger shoot length per explant, while the concentration of 1.0 mg L-1 of kinetin promoted larger shoot length in general. GA3 did not affect the elongation of pepper-rosmarin shoots when cultivated in vitro.

scholarly journals Multiplicação in vitro de Caesalpinia pyramidalis (Leguminosae)

2013 ◽  
Vol 13 ◽  
Author(s):  
Tecla Dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Bárbara Paula dos Santos Borges ◽  
Bruno Freitas Matos Alvim ◽  
José Raniere Ferreira De Santana
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Dry Mass ◽  
Shoot Length ◽  
Nodal Segments ◽  
In Vitro Multiplication ◽  
Different Types

Caesalpinia pyramidalis is a species endemic to the Caatinga and known popularly as catingueira, which is widely used by local people, mainly for its timber and medicinal and fodder properties. This study investigated the effects of different types and concentrations of plant growth regulators on the in vitro multiplication of C. pyramidalis. In the first experiment, nodal segments were inoculated in media containing different combinations (0.0–8.0 µM) of BAP and NAA. In the second experiment, nodal segments wereinoculated in media containing different types (KIN, BAP and TDZ) and concentrations (0.0–16μM) of cytokinins. We used a WPM medium supplemented with 87.64 mM sucrose and solidified with 7.0 g L-1 agar. After 45 days, the highest number of shoots, leaf number, shoot length and dry mass of shoots were obtained when nodal segments were inoculated into a culture medium without plant growth regulators.

scholarly journals LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

2018 ◽  
Vol 42 (6) ◽  
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
Natane Amaral Miranda ◽  
Joane Helena Maggioni
Keyword(s):  
Light Source ◽  
Culture Medium ◽  
Light Quality ◽  
Shoot Length ◽  
Nodal Segments ◽  
Fluorescent Lamp ◽  
Corymbia Citriodora ◽  
Hybrid Clones ◽  
Effect Of Light

ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.

scholarly journals DESINFESTAÇÃO E MEIO DE CULTURA PARA O ESTABELECIMENTO In Vitro DE SEGMENTOS NODAIS DE Liquidambar styraciflua

FLORESTA ◽  
2010 ◽  
Vol 40 (3) ◽  
Author(s):  
Gilvano Ebling Brondani ◽  
Fabricio Augusto Hansel ◽  
Leonardo Ferreira Dutra ◽  
Ivar Wendling
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Contamination ◽  
Culture Medium ◽  
Morphological Characteristics ◽  
Liquidambar Styraciflua ◽  
Nodal Segments ◽  
Hydroponic System ◽  
Randomized Design ◽  
In Vitro Establishment

O objetivo deste trabalho foi testar a desinfestação e meios de cultura para o estabelecimento in vitro de segmentos nodais de Liquidambar styraciflua L. Os explantes foram coletados de minicepas propagadas pelo processo de estaquia e manejadas em minijardim clonal sob sistema semi-hidropônico em leito de areia. O experimento foi conduzido em delineamento inteiramente casualizado no arranjo fatorial (3x2x2), sendo os fatores constituídos por três clones (L 26, L 35 e L 63), duas metodologias de desinfestação (A1 - hipoclorito de sódio (NaOCl) durante 10 minutos a 2,5% v/v de cloro ativo e A2 - explantes mergulhados durante 40 minutos em solução a base de benomyl à 1% p/v) e dois meios de cultura (WPM e MS), com quatro repetições. Os clones não diferiram em relação à assepsia e meio de cultura, obtendo-se média de 4% de explantes isentos de contaminação. Contudo, cerca de 80% dos explantes apresentaram contaminação bacteriana, indicando a necessidade do desenvolvimento de um protocolo de desinfestação mais eficiente. Embora tenham ocorrido poucas diferenças entre os meios de cultura testados, o meio de cultura MS apresentou superioridade em relação ao WPM para a maioria das características morfológicas estudadas.Palavras-chave: Micropropagação; assepsia; contaminação bacteriana; hipoclorito de sódio; benomyl. AbstractDisinfestation and culture medium for the in vitro establishment of Liquidambar styraciflua nodal segments. The objective of this research was to test the culture medium sterilization for the in vitro establishment of Liquidambar styraciflua L. nodal segments. Ministumps, from which the explants were collected, were propagated by cutting process and managed in clonal mini garden under semi-hydroponic system in a sand bed. The experiment was conducted in a completely randomized design under factorial arrangement (3x2x2); the factors were: three clones (L 26, L 35 and L 63), two sterilization methods (A1 - sodium hypochlorite (2.5% v/v of active chlorine) during 10 minutes; A2 - explants immersed during 40 minutes in a solution of benomyl 1% w/v) and two culture mediums (WPM and MS), with four replications. The clones did not differ in relation to the asepsis and culture medium. In average, 4% of the explants were free of contamination. However, almost 80% of the material presented bacterial contamination, what indicated the necessity of developing a more efficient sterilization protocol. Although only a little difference between the culture mediums had been observed, the MS medium showed superiority in relation to the WPM regarding the majority of the morphological characteristics studied.Keywords: Micropropagation; asepsis; bacterial contamination; sodium hypochlorite; benomyl.

scholarly journals In vitro cultivation of Tamarindus indica L.: explants obtention and contamination in culture medium

2018 ◽  
Vol 9 (2) ◽  
pp. 298-302
Author(s):  
Antonio Flávio Arruda Ferreira ◽  
Laís Naiara Honorato Monteiro ◽  
Maria Gabriela Fontanetti Rodrigues ◽  
Natália Batista Oliveira ◽  
Aparecida Conceição Boliani
Keyword(s):  
Activated Charcoal ◽  
Culture Medium ◽  
Test Tube ◽  
Nodal Segments ◽  
In Vitro Cultivation ◽  
Tamarindus Indica ◽  
Randomized Design ◽  
In Vitro Establishment ◽  
Sexual Propagation

The tamarind tree (Tamarindus indica L.) is a common tree in tropical countries with a great exploitation potential due to its high nutritional value and important pharmaceutical characteristics, justifying its potential as a promising crop. The scarcity of scientific studies of the species, especially on propagation, hinders its availability and, consequently, the supply of the product in the market. The aim of this study was to verify the obtainment of nodal segments via sexual propagation and the in vitro establishment of sweet tamarind in MS culture medium (25, 50, 75 and 100% of salts) and with or without activated charcoal (2 g.L-1). The experiment was carried out in a completely randomized design in a 2 x 4 factorial scheme (presence and absence of activated carbon x salt concentrations), with 25 replicates, each replicate consisting of a test tube with an inoculated explant. According to the results, it is possible to conclude that from seedlings with 45 days after sowing, nodal segments of sweet tamarind are obtained for in vitro establishment. As a precursor of protocol for in vitro formation of healthy seedlings is indicated the use of MS culture medium with 75 % of the salts and added with 2 gL-1 of activated charcoal to reduce the contamination index.

scholarly journals Auxin Prompted Improved Micropropagation Protocol of Picrorhiza kurroa: An Endangered Medicinal Plant

1970 ◽  
Vol 19 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Arif Jan ◽  
Phalisteen S ◽  
G.T. Thomas ◽  
A. S. Shawl
Keyword(s):  
Medicinal Plant ◽  
Culture Medium ◽  
Root Formation ◽  
Normal Development ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Picrorhiza Kurroa ◽  
Endangered Medicinal Plant ◽  
Mother Plants

In vitro shoot multiplication from nodal explants of Picrorhiza kurroa Royle ex. Benth. (Kour) was obtained on MS without cytokinin. Addition of NAA in the culture medium favours shoots as well as root formation. Among the various combinations of MS and B5 media, the average number of shoots per explant was (18.3) on MS supplemented with 0.6 mg/l NAA. Shoot length was maximum (5.8 cms) in MS augmented with 0.4 mg/l  NAA. The elongated shoots rooted within 15 - 25 days either on MS alone or supplemented with NAA (0.4 mg/l), IAA and IBA (0.1 - 0.5 mg/l). About 81.5% of the rooted plantlets survived acclimation and transferred to the greenhouse. All the in vitro derived plants at maturity displayed normal development as that of the mother plants at their maturity. Key words: Picrorhiza kurroa, Hardening, Medicinal plant, Propagation D.O.I. 10.3329/ptcb.v19i2.5433 Plant Tissue Cult. & Biotech. 19(2): 161-167, 2009 (December)

scholarly journals In vitro propagation of an economically important medicinal plant Lawsonia inermis L. through nodal segments

2021 ◽  
Vol 13 (3) ◽  
pp. 897-906
Author(s):  
Amit ◽  
Rajkumar ◽  
Narender Singh
Keyword(s):  
Medicinal Plant ◽  
Growth Regulators ◽  
Nodal Segments ◽  
Ms Medium ◽  
Lawsonia Inermis ◽  
Indole Butyric Acid ◽  
Mass Multiplication ◽  
Half Strength ◽  
Important Medicinal Plant

The present investigation aimed to standardize efficient plant regeneration protocol through in vitro culture by using nodal segment for mass multiplication of Lawsonia inermis an economically important medicinal plant species. Mass multiplication of shoots induced on Murashige and Skoog (MS) medium supplemented with different growth regulators like auxins and cytokinins separately and in different combinations. The medium fortified with 6-Benzylaminopurine ( BAP) 1.0 mg/l + kinetin (KN) 1.5mg/l  explained best compared to all other combinations. In vitro raised plantlets were excised and transferred in half strength MS  medium supplemented with different growth regulators like Indole Butyric acid ( IBA)  and naphthalene acetic acid (NAA ) (0.5-3.0 mg/l) in an experiment that gave rise to rooting. The half strength of MS medium additive with IBA in separate and in different combinations with NAA concentrations (0.5-3.0 mg/l) supported root development. The best response of rooting was obtained on half MS medium fortified with 1.0 mg/l IBA. The regenerated plantlets were successfully transplanted to pots. Regenerants were transferred to the field conditions and recorded the survival rate.. Among all the carbon sources and gelling agents used, sucrose (3%) in combination with 0.8 per cent agar-agar has proved significantly better. Multiple shoots formation with longer shoots were achieved on medium with 1.0mg/l BAP and 1.5mg/l Kn. Thus, it is possible to develop a large number of plants of L. inermis through shoot bud regeneration which can cater for the need of pharmaceutical as well as other industries.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties

2012 ◽  
Vol 11 (1) ◽  
pp. 102 ◽  
Author(s):  
Zied Zarai ◽  
Ines Chobba ◽  
Riadh Mansour ◽  
Ahmed Békir ◽  
Néji Gharsallah ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ricinus Communis ◽  
Antimicrobial Properties ◽  
In Vitro Cytotoxicity ◽  
Ricinus Communis L
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