scholarly journals Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

1986 ◽  
Vol 28 (2) ◽  
pp. 80-86 ◽  
Author(s):  
N. Yoshida ◽  
M. M. G. Teixeira ◽  
C. A. Sbravate
Keyword(s):  
Monoclonal Antibody ◽  
Trypanosoma Cruzi ◽  
Surface Protein ◽  
Metacyclic Trypomastigotes ◽  
Vector Borne ◽  
Axenic Cultures ◽  
Cruzi Infection

Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.

scholarly journals Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection

1987 ◽  
Vol 82 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Mauro Schechter
Keyword(s):  
Monoclonal Antibody ◽  
Trypanosoma Cruzi ◽  
Affinity Chromatography ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Monoclonal Antibody ◽  
Antibody Affinity ◽  
Specific Diagnosis ◽  
Purified Antigen ◽  
Cruzi Infection

Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from and infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

scholarly journals Characterization of cardiopulmonary function and cardiac muscarinic and adrenergic receptor density adaptation in C57BL/6 mice with chronic Trypanosoma cruzi infection

Parasitology ◽  
2006 ◽  
Vol 133 (06) ◽  
pp. 729 ◽  
Author(s):  
N. N. ROCHA ◽  
S. GARCIA ◽  
L. E. D. GIMÉNEZ ◽  
C. C. Q. HERNÁNDEZ ◽  
J. F. V. SENRA ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Adrenergic Receptor ◽  
Receptor Density ◽  
Cardiopulmonary Function ◽  
Cruzi Infection

Protective Immunity Against Trypanosoma cruzi Infection in a Highly Susceptible Mouse Strain After Vaccination with Genes Encoding the Amastigote Surface Protein-2 and Trans-Sialidase

2004 ◽  
Vol 15 (9) ◽  
pp. 878-886 ◽  
Author(s):  
José Ronnie Vasconcelos ◽  
Meire I. Hiyane ◽  
Cláudio R.F. Marinho ◽  
Carla Claser ◽  
Alexandre M.V. Machado ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Mouse Strain ◽  
Protective Immunity ◽  
Surface Protein ◽  
Susceptible Mouse ◽  
Genes Encoding ◽  
Cruzi Infection

cDNA cloning and partial characterization of amastigote specific surface protein from Trypanosoma cruzi

2009 ◽  
Vol 9 (6) ◽  
pp. 1083-1091 ◽  
Author(s):  
Marybell Olivas-Rubio ◽  
Salvador Hernández-Martínez ◽  
Patricia Talamás-Rohana ◽  
Victor Tsutsumi ◽  
Pedro A. Reyes-López ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Specific Surface ◽  
Cdna Cloning ◽  
Surface Protein ◽  
Partial Characterization

Immunization with the Recombinant Surface Protein rTcSP2 Alone or Fused to the CHP or ATPase Domain of TcHSP70 Induces Protection Against Acute Trypanosoma cruzi Infection

2010 ◽  
Vol 01 (03) ◽  
Author(s):  
Alejandro Carabarin-Lima ◽  
María Cristina González- Vázquez ◽  
Baylon-Pacheco Lidia ◽  
Patricia-Talamas Rohana ◽  
Rosales-Encina José Luis
Keyword(s):  
Trypanosoma Cruzi ◽  
Surface Protein ◽  
Atpase Domain ◽  
Cruzi Infection

scholarly journals Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi

FEBS Letters ◽  
1985 ◽  
Vol 180 (2) ◽  
pp. 265-270 ◽  
Author(s):  
S. Goldenberg ◽  
J.M. Salles ◽  
V.T. Contreras ◽  
M.P.Lima Franco ◽  
A.M. Katzin ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Messenger Rna ◽  
Metacyclic Trypomastigotes

scholarly journals Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61795 ◽  
Author(s):  
Rafael Polidoro Alves Barbosa ◽  
Bruno Galvão Filho ◽  
Luara Isabela dos Santos ◽  
Policarpo Ademar Sales Junior ◽  
Pedro Elias Marques ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Surface Protein ◽  
Highly Effective ◽  
Cruzi Infection
Sign in / Sign up

Export Citation Format

Share Document