scholarly journals EPIDEMIOLOGICAL SURVEY ON CANINE POPULATION WITH THE USE OF IMMUNOLEISH SKIN TEST IN ENDEMIC AREAS OF HUMAN AMERICAN CUTANEOUS LEISHMANIASIS IN THE STATE OF RIO DE JANEIRO, BRAZIL

1998 ◽  
Vol 40 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Elizabeth Gloria O. Barbosa Santos ◽  
Mauro Célio A. Marzochi ◽  
Nilton Francisco Conceição ◽  
Célia Maria M. Brito ◽  
Raquel S. Pacheco
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Rio De Janeiro ◽  
Epidemiological Survey ◽  
The State ◽  
Clinical Aspects ◽  
Mucocutaneous Leishmaniasis ◽  
American Cutaneous Leishmaniasis ◽  
Epidemiological Surveys ◽  
Positive Rate

A survey for canine tegumentary leishmaniasis (CTL) has been carried out between 1986 and 1993 in seven endemic localities for American cutaneous leishmaniasis in the State of Rio de Janeiro. 270 dogs have been examined for their clinical aspects, the development of delayed hypersensitivity (DHS) with Immunoleish antigen and with immunofluorescent antibody research of IgG (IF). 28.2% of them had ulcer lesions and 3.3% had scars. The lesions consisted of single (39.5%) and mucocutaneous lesions (31.6%), multiple cutaneous (25.0%) and mucocutaneous lesions associated with cutaneous ulcers (4.0%). Twelve (15.8%) isolates from biopsies were analyzed by zimodeme and schizodeme and identified as L. (V.) braziliensis. The overall prevalence of canine infection that was evaluated with the skin test was of 40.5% and with IF it was of 25.5%. Both tests showed a high positive rate with relation to the animals with mucosal lesions, as in the case of human mucocutaneous leishmaniasis. The comparison of the two tests showed the skin test to have a better performance although there was no statistical difference (p>0.05) between them. The proportional sensitivity and specificity was of 84.0% and 74.0%, respectively. The Immunoleish skin test and IF are useful tools to be employed in CTL field epidemiological surveys.

scholarly journals Clinical, epidemiological and laboratory aspects of patients with American cutaneous leishmaniasis in the State of Pernambuco

2008 ◽  
Vol 41 (5) ◽  
pp. 439-443 ◽  
Author(s):  
Luiza de Campos Reis ◽  
Maria Edileuza Felinto de Brito ◽  
Éricka Lima de Almeida ◽  
Simone Marta Félix ◽  
Ângela Cristina Rapela Medeiros ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Indirect Immunofluorescence ◽  
The State ◽  
Chain Reaction ◽  
American Cutaneous Leishmaniasis ◽  
Polymerase Chain ◽  
Epidemiological Characteristics ◽  
Direct Investigation

The diagnosis for American cutaneous leishmaniasis is based on an association of clinical, epidemiological and laboratory characteristics. The present study identified the circulating species of Leishmania in the State of Pernambuco, described its clinical-epidemiological characteristics and diagnosed the disease. Nineteen patients presenting active lesions who had been diagnosed through clinical evaluation and laboratory tests were selected. The tests included direct investigation, in vitro culturing, Montenegro skin test, indirect immunofluorescence and polymerase chain reaction. The Montenegro Skin Test showed positive results in 89% of the patients; indirect immunofluorescence, in 79%; direct investigation, in 58%; and polymerase chain reaction in 75%. Seven Leishmania (Viannia) braziliensis samples were isolated from these patients and were characterized by means of specific monoclonal antibodies. These data confirm that a combination of different diagnosis techniques is needed in order to obtain efficient results and that, so far, Leishmania (Viannia) braziliensis is the only species responsible for American cutaneous leishmaniasis infection in Pernambuco. Thus, it is essential to identify the parasite species involved in cases of human disease in an endemic area in order to determine the clinical and epidemiological characteristics, especially with regard to diagnosis, therapy development and disease prognosis.

Montenegro skin tests for American cutaneous leishmaniasis carried out on school children in Rio de Janeiro, Brazil: An indicator of transmission risk

Acta Tropica ◽  
1992 ◽  
Vol 52 (2-3) ◽  
pp. 111-119 ◽  
Author(s):  
Wilson J.S. Souza ◽  
Paulo C. Sabroza ◽  
Carlos S. Santos ◽  
Edilson de Sousa ◽  
Marcia F. Henrique ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
School Children ◽  
Rio De Janeiro ◽  
Transmission Risk ◽  
Skin Tests ◽  
American Cutaneous Leishmaniasis

scholarly journals Epidemiological profile of cutaneous leishmaniasis in an endemic region in the State of Rio de Janeiro, Brazil

2009 ◽  
Vol 18 (03) ◽  
pp. 34-40 ◽  
Author(s):  
M. C. F. S. de Bustamante ◽  
M. J. S. Pereira ◽  
A. de O. Schubach ◽  
A. H. da Fonseca
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Rio De Janeiro ◽  
The State ◽  
Endemic Region ◽  
Epidemiological Profile

scholarly journals Histological observations on Montenegro's reaction in man

1989 ◽  
Vol 31 (4) ◽  
pp. 256-261 ◽  
Author(s):  
Wilson Mayrink ◽  
Antonio Pedro M. Schettini ◽  
Paul Williams ◽  
Pedro Raso ◽  
Paulo Araujo Magalhães ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Test ◽  
Diagnostic Method ◽  
Previous History ◽  
Histological Changes ◽  
Histological Studies ◽  
Vaccine Trials ◽  
American Cutaneous Leishmaniasis ◽  
History Of

The Montenegro skin test is widely used as a diagnostic method for American cutaneous leishmaniasis (ACL) but little is known about the histological changes that occur in the skin after administration of the antigen. This report is based on histological studies of biopsied material obtained, from inoculation sites, 48 hours after individuals had been given intradermal injections with a standardized Montenegro antigen. The material examined was obtained from four distinctly different test groups: naturally infected patients with parasitologically proved ACL and with positive Montenegro's reaction; individuals without previous history of ACL and not previously tested with Montenegro antigen; participants in anti-ACL vaccine trials who developed positive reactions to Montenegro antigen after vaccination; other participants in vaccine trials who had negative Montenegro responses after vaccination or had served as controls in the trials. The histological pictures of each group are described and discussed. Histologically, the reactions of vaccinated individuals were indistinguishable from those with naturally acquired infections.

scholarly journals Epidemiological survey of Neorickettsia risticii in equids from the State of Rio de Janeiro, Brazil

2016 ◽  
Vol 36 (10) ◽  
pp. 939-946 ◽  
Author(s):  
Erica C.R. Roier ◽  
◽  
Renata L. Costa ◽  
Marcus S. Pires ◽  
Joice A.R. Vilela ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Multiple Logistic Regression Analysis ◽  
Epidemiological Survey ◽  
Immunofluorescence Assay ◽  
The State ◽  
Seropositivity Rate ◽  
Age Related ◽  
Target Dna ◽  
Potomac Horse Fever ◽  
Low Altitude

ABSTRACT: Equine neorickettsiosis (EN), also known as Potomac Horse Fever, is a non-contagious disease caused by the bacterium Neorickettsia risticii of the Anaplasmataceae family. The objectives of this study were to detect the presence of anti-N. risticii antibodies by the indirect immunofluorescence assay (IFA) and of its DNA by qPCR in equids at high and low altitude regions in the State of Rio de Janeiro, Brazil, and to identify factors associated with seropositive equids by multiple logistic regression analysis. The frequency of anti-N. risticii antibodies was 16.05% (n=113/704). The animal age and breeding region were the factors that influenced the seropositivity rate for N. risticii in the equids (p<0.05). Equids from the lowland region had higher seropositivity (p<0.05; OR=5.87) compared to those of the mountain region. The presence of snails on the farm was a factor associated with this result (p<0.05; OR=2.88). In the lowland region, age of the animal and site of breeding were protective factors for the detection of antibodies anti-N. risticii in equids, with lower frequency of seropositivity in younger animals (p<0.05; OR=0.06) and in animals raised in dry areas (p<0.05; OR=0.22). The presence of the target DNA of N. risticii by qPCR was not observed in any of the samples tested. The existence of seropositive equids for N. risticii demonstrates a possible circulation of this agent in the studied area, and that the age related characteristics and equids breeding region are important factors regarding seropositivity in the State of Rio de Janeiro.

scholarly journals An analysis of the spatiotemporal distribution of American cutaneous leishmaniasis in counties located along road and railway corridors in the State of Maranhao, Brazil

2013 ◽  
Vol 46 (3) ◽  
pp. 322-328 ◽  
Author(s):  
Vicente Silva Goncalves Neto ◽  
Allan Kardec Duailibe Barros Filho ◽  
Alcione Miranda dos Santos ◽  
Margarida Paula Carreira de Sa Prazeres ◽  
Ana Celia Rolim Bezerril ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
The State ◽  
Spatiotemporal Distribution ◽  
American Cutaneous Leishmaniasis

scholarly journals Low versus high dose of antimony for American cutaneous leishmaniasis: A randomized controlled blind non-inferiority trial in Rio de Janeiro, Brazil

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0178592 ◽  
Author(s):  
Mauricio Naoto Saheki ◽  
Marcelo Rosandiski Lyra ◽  
Sandro Javier Bedoya-Pacheco ◽  
Liliane de Fátima Antônio ◽  
Maria Inês Fernandes Pimentel ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Rio De Janeiro ◽  
High Dose ◽  
Randomized Controlled ◽  
American Cutaneous Leishmaniasis
Sign in / Sign up

Export Citation Format

Share Document