Ecophysiology of the kleptoplastidic dinoflagellate Shimiella gracilenta: I. spatiotemporal distribution in Korean coastal waters and growth and ingestion rates

To explore the ecophysiological characteristics of the kleptoplastidic dinoflagellate Shimiella gracilenta, we determined its spatiotemporal distribution in Korean coastal waters and growth and ingestion rates as a function of prey concentration. The abundance of S. gracilenta at 28 stations from 2015 to 2018 was measured using quantitative realtime polymerase chain reaction. Cells of S. gracilenta were detected at least once at all the stations and in each season, when temperature and salinity were 1.7–26.4°C and 9.9–35.6, respectively. Moreover, among the 28 potential prey species tested, S. gracilenta SGJH1904 fed on diverse prey taxa. However, the highest abundance of S. gracilenta was only 3 cells mL-1 during the study period. The threshold Teleaulax amphioxeia concentration for S. gracilenta growth was 5,618 cells mL-1, which was much higher than the highest abundance of T. amphioxeia (667 cells mL-1). Thus, T. amphioxeia was not likely to support the growth of S. gracilenta in the field during the study period. However, the maximum specific growth and ingestion rates of S. gracilenta on T. amphioxeia, the optimal prey species, were 1.36 d-1 and 0.04 ng C predator- 1 d-1, respectively. Thus, if the abundance of T. amphioxeia was much higher than 5,618 cells mL-1, the abundance of S. gracilenta could be much higher than the highest abundance observed in this study. Eurythermal and euryhaline characteristics of S. gracilenta and its ability to feed on diverse prey species and conduct kleptoplastidy are likely to be responsible for its common spatiotemporal distribution.

Review on Atmospheric Ozone Pollution in China: Formation, Spatiotemporal Distribution, Precursors and Affecting Factors

In recent years, atmospheric ozone pollution has become more and more serious in many areas of China due to the rapid development of industrialization and urbanization. The increase in atmospheric ozone concentration will not only cause harm to the human respiratory tract, nervous system and immune system, but also cause obvious harm to crops, which will lead to reductions in crop production. Therefore, the study of atmospheric ozone pollution should not be ignored in research on the atmospheric environment. In this paper, we summarized the formation mechanisms of atmospheric ozone, the spatiotemporal distribution characteristics of atmospheric ozone in some areas of China, the relationship between atmospheric ozone and its precursors, and the main factors affecting the concentration of atmospheric ozone. Then, the control countermeasures against atmospheric ozone pollution were put forward in combination with the actual situation in China.

Framework for Monitoring the Spatiotemporal Distribution and Clustering of Drought Characteristics in Hunan Province

Drought is a widespread phenomenon in the context of global climate change. Owing to the geographical location of Hunan Province in the middle reaches of Yangtze River and the abundance of forests area in this region with a large population, there is a need to focus on the impacts of drought for devising policies. The spatiotemporal distribution scheme of a given area must be determined to plan water management and protect ecosystems effectively. This study proposes a framework for exploring the spatiotemporal distribution model of drought using comprehensive surveys of historical meteorological stations, which consists of two parts, namely the characteristics of drought extraction in the spatiotemporal distribution and drought models discovered by the clustering method. Firstly, we utilized the run theory to extract drought characteristics, such as drought duration, drought severity, and drought intensity. Secondly, the K-means clustering method was adopted to explore the distribution patterns on the basis of the drought characteristics. Lastly, the method was applied to Hunan Province. Results show that historical drought conditions can be monitored with their characteristics of spatiotemporal variability. Three drought distribution clusters exist in this region. Cluster 1 in western Hunan tends to be a long-term, low-intensity drought, cluster 2 in the southern part tends to be a short-term, high-intensity drought, and cluster 3 in the central part is prone to severe drought. The proposed framework is flexible as it allows parameters to be adjusted and extraction methods to achieve reasonable results for a given area.

Visualizing Spatiotemporal Epidemic Clusters on a Map-based Dashboard: A case study of early COVID-19 cases in Singapore

Spatiotemporal distribution of the epidemic data plays an important role in its understanding and prediction. In order to understand the transmission patterns of infectious diseases in a more intuitive way, many works applied various visualizations to show the epidemic datasets. However, most of them focus on visualizing the epidemic information at the overall level such as the confirmed counts each country, while spending less effort on powering user to effectively understand and reason the very large and complex epidemic datasets through flexible interactions. In this paper, the authors proposed a novel map-based dashboard for visualizing and analyzing spatiotemporal clustering patterns and transmission chains of epidemic data. We used 102 confirmed cases officially reported by the Ministry of Health in Singapore as the test dataset. This experiment shown that the well-designed and interactive map-based dashboard is effective in shorten the time that users required to mine the spatiotemporal characteristics and transmission chains behind the textual and numerical epidemic data.

Unraveling the Spatiotemporal Distribution of VPS13A in the Mouse Brain

Loss-of-function mutations in the human vacuolar protein sorting the 13 homolog A (VPS13A) gene cause Chorea-acanthocytosis (ChAc), with selective degeneration of the striatum as the main neuropathologic feature. Very little is known about the VPS13A expression in the brain. The main objective of this work was to assess, for the first time, the spatiotemporal distribution of VPS13A in the mouse brain. We found VPS13A expression present in neurons already in the embryonic stage, with stable levels until adulthood. VPS13A mRNA and protein distributions were similar in the adult mouse brain. We found a widespread VPS13A distribution, with the strongest expression profiles in the pons, hippocampus, and cerebellum. Interestingly, expression was weak in the basal ganglia. VPS13A staining was positive in glutamatergic, GABAergic, and cholinergic neurons, but rarely in glial cells. At the cellular level, VPS13A was mainly located in the soma and neurites, co-localizing with both the endoplasmic reticulum and mitochondria. However, it was not enriched in dendritic spines or the synaptosomal fraction of cortical neurons. In vivo pharmacological modulation of the glutamatergic, dopaminergic or cholinergic systems did not modulate VPS13A concentration in the hippocampus, cerebral cortex, or striatum. These results indicate that VPS13A has remarkable stability in neuronal cells. Understanding the distinct expression pattern of VPS13A can provide relevant information to unravel pathophysiological hallmarks of ChAc.

Spatiotemporal distribution of the temperature field inside the thin heated foil during impacting liquid spray

The spatiotemporal distribution of the temperature inside a constantan foil during impacting spray is resolved experimentally in the present work. The received infrared image sequence will be used to find the local and average heat transfer coefficient of the foil. In the future, the results obtained will be used to calculate the heat flux in the region of the contact line of each drop.