A comparative study for production of germ tube in Candida albicans of various pulmonary samples, by different methods in a tertiary care hospital of south west Rajasthan

Background: Systemic candidiasis is associated with a high crude mortality rate, even with first line antifungal therapy. C. albicans is the predominant cause of invasive fungal diseases which is a serious public health issue. The main objective was to assess the reliability of different media for germ tube production in Candida albicans isolated from various clinically diagnosed pulmonary samples.Methods: All Candida isolates were identified and speciated by conventional methods such as Gram’s staining, germ tube test, chlamydospore formation on corn meal agar, sugar fermentation test, sugar assimilation test, and growth on Hi-chrome candida agar.Results: Out of 108 clinical isolates of Candida albicans, 5 different methods were used for germ tube production. Pooled human sera showed 93/108 (86.1%) was the most sensitive method wherein YEPD (yeast extract peptone dextrose) broth 91/108 (84.7%) was the reliable and easy method for detection of germ tube, followed by trypticase soy broth 81/108 (81.4%); peptone water 80/108 (74.7%) and 2% sucrose 71/108 (65.7%).Conclusions: YPED broth is found to be a better serum free substrate and subsequently for the presumptive differentiation of C. albicans from non-albicans candida (NAC), without the extensive time required for the preparation and testing of pooled human serum. Furthermore, this medium is commercially available, more stable, effective, and is not bio hazardous.

Fast Blob and Air Line Defects Detection for High Speed Glass Tube Production Lines

During the production of pharmaceutical glass tubes, a machine-vision based inspection system can be utilized to perform the high-quality check required by the process. The necessity to improve detection accuracy, and increase production speed determines the need for fast solutions for defects detection. Solutions proposed in literature cannot be efficiently exploited due to specific factors that characterize the production process. In this work, we have derived an algorithm that does not change the detection quality compared to state-of-the-art proposals, but does determine a drastic reduction in the processing time. The algorithm utilizes an adaptive threshold based on the Sigma Rule to detect blobs, and applies a threshold to the variation of luminous intensity along a row to detect air lines. These solutions limit the detection effects due to the tube’s curvature, and rotation and vibration of the tube, which characterize glass tube production. The algorithm has been compared with state-of-the-art solutions. The results demonstrate that, with the algorithm proposed, the processing time of the detection phase is reduced by 86%, with an increase in throughput of 268%, achieving greater accuracy in detection. Performance is further improved by adopting Region of Interest reduction techniques. Moreover, we have developed a tuning procedure to determine the algorithm’s parameters in the production batch change. We assessed the performance of the algorithm in a real environment using the “certification” functionality of the machine. Furthermore, we observed that out of 1000 discarded tubes, nine should not have been discarded and a further seven should have been discarded.

In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract

Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different Candida spp., and their influence on virulence factors of C. albicans (germ tube production, gene expression for ALS3, SAP2, HSP70). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied Candida spp., but biofilm production was inhibited only for C. albicans and C. guilliermondii. The growth rates of all the studied Candida spp. were inhibited in the presence of both AgNP BBEs, except for C. auris. Synergistic activity was observed for C. parapsilosis and C. guilliermondii, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of C. albicans was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of SAP2. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments.

Analysis of the Drawing Process of Small-Sized Seam Tubes

This article is focused on an analysis of factors negatively affecting the tube production process of tubes made from austenitic stainless steel with a very small diameter of ϕ 0.34 mm. The analysis was concentrated on factors that affect the drawing process stability of the seam tubes where the desired final dimensions—a diameter of ϕ 0.34 mm and a wall thickness of 0.057 mm—are limiting factors. Seam tubes made from steel 1.4306 and 1.4301, from producers KT and EW with a longitudinal weld line made by tungsten inert gas (TIG) welding, were used as blanks for constituent drawing operations. It is desirable to provide sufficient inert gas flow and cooling during the formation of a weld joint in a protective atmosphere chamber. A significant temperature gradient prevents the formation of undesirable Cr23C6 carbides in the heat-affected zone (HAZ) which negatively affects the plasticity and formability of the steel and is the cause of technological fractures.

Influence of Texture on Mechanical Properties of Titanium Alloy Tubes

Mechanical properties and texture of tube material formed in the process of tube production were studied. Texture in tubes was estimated by contractile strain ratio (CSR) according to requirements of the standard for tubes. Mechanical properties depend on CSR. Optimal values of mechanical properties were determined: ultimate strength, yield strength and relative elongation which provide CSR values within 1.3...3.5. The texture was evaluated in the process of tube manufacture. An increase in radial component of the texture leads to an increase in CSR. At high CSR values up to 3-3.5, tubes often do not withstand process tests for flattening and bending. Correction of CSR values is required. It was shown that an increase in ductility of tubes increases the value of CSR.