scholarly journals MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL

2015 ◽  
Vol 57 (4) ◽  
pp. 343-347 ◽  
Author(s):  
Carolina Bioni Garcia TELES ◽  
Jansen Fernandes MEDEIROS ◽  
Ana Paula de Azevedo dos SANTOS ◽  
Luís Antônio Rodrigues de FREITAS ◽  
Tony Hiroshi KATSURAGAWA ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Rural Areas ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Predominant Species ◽  
American Cutaneous Leishmaniasis ◽  
Polymerase Chain ◽  
Urban And Rural Areas ◽  
Rural Work

SUMMARY In this study, Leishmaniaspecies were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia triborder was also investigated. By PCR, the DNA of Leishmaniawas detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%) , L. (V.) shawi (28.1%) , L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis (3.1%)This is the first report of L. (V.) shawiand L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting.

scholarly journals A serological and molecular investigation of American cutaneous leishmaniasis in dogs, three years after an outbreak in the Northwest of Paraná State, Brazil

2009 ◽  
Vol 25 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Gustavo Kiyoshi Massunari ◽  
Evandra Maria Voltarelli ◽  
Demilson Rodrigues dos Santos ◽  
Ademar Rodrigues dos Santos ◽  
Luiz Paschoal Poiani ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cutaneous Leishmaniasis ◽  
Control Measures ◽  
Transmission Control ◽  
Chain Reaction ◽  
American Cutaneous Leishmaniasis ◽  
Molecular Investigation ◽  
Polymerase Chain ◽  
Pcr Techniques

Classic and molecular (polymerase chain reaction - PCR) techniques were used to diagnose American cutaneous leishmaniasis in 149 dogs from an area in the northwest of Paraná State, Brazil, where an American cutaneous leishmaniasis outbreak occurred in 2002. The results were compared to a set of previously obtained results. Twenty-five dogs had positive indirect immunofluorescence (IIF) (titers > 40), including two animals with suggestive lesions. The percentage of dogs with positive IIF was similar to that found in a previous study. The cultures of the lesion, blood and bone marrow were negative for Leishmania. A direct search for the parasite in the lesions proved negative, although PCR tests were positive. The PCR did not detect the DNA of Leishmania (Viannia) in the blood, even for those that had positive PCR in a previous study. The follow up of the 27 dogs showed that the majority of them had maintained the same levels of antibodies that had been detected previously. There was a reduction in the number of dogs with lesions, probably due to the transmission control measures that were adopted after the outbreak.

scholarly journals Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis

2006 ◽  
Vol 54 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Marcos J. Marques ◽  
Ângela C. Volpini ◽  
George L.L. Machado-Coelho ◽  
Jackson Machado-Pinto ◽  
Carlos A. da Costa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cutaneous Leishmaniasis ◽  
Chain Reaction ◽  
Laboratory Methods ◽  
American Cutaneous Leishmaniasis ◽  
Polymerase Chain

scholarly journals Characterization of phytoplasmas related to 'Candidatus Phytoplasma asteris' subgroup rpI-L in Iran

2014 ◽  
Vol 54 (2) ◽  
pp. 199-203 ◽  
Author(s):  
Fereshteh Vali-Sichani ◽  
Masoud Bahar ◽  
Leila Zirak
Keyword(s):  
Fragment Length Polymorphism ◽  
Specific Primer ◽  
Sequence Analyses ◽  
Chain Reaction ◽  
Universal Primer ◽  
Disease Symptoms ◽  
Polymerase Chain ◽  
Pcr Assays ◽  
Niger Seed

Abstract In two of Iran's central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect 'Canididatus Phytoplasma asteris'-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/ R16R2 in nested PCR. Then, for detection of 'Ca. P. asteris', DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp509I restriction enzyme as well as sequence analyses indicated that 'Ca. P. asteris'-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rpI-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rpI-L subgroup.

scholarly journals Characterization of a Phytoplasma Associated with Frogskin Disease in Cassava

Plant Disease ◽  
2009 ◽  
Vol 93 (11) ◽  
pp. 1139-1145 ◽  
Author(s):  
Elizabeth Alvarez ◽  
Juan F. Mejía ◽  
Germán A. Llano ◽  
John B. Loke ◽  
Alberto Calari ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Manihot Esculenta ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
South American ◽  
Sequence Analyses ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively assigned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia.

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