scholarly journals A simplified method for sample collection and DNA isolation for polymerase chain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors

2000 ◽  
Vol 95 (6) ◽  
pp. 863-866 ◽  
Author(s):  
Evandro MM Machado ◽  
Nelson J Alvarenga ◽  
Alvaro J Romanha ◽  
Edmundo C Grisard
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Dna Isolation ◽  
Sample Collection ◽  
Polymerase Chain Reaction Detection ◽  
Trypanosoma Rangeli ◽  
Chain Reaction ◽  
Simplified Method ◽  
Polymerase Chain

scholarly journals Polymerase Chain Reaction Detection of Trypanosoma cruzi in Macaca fascicularis Using Archived Tissues

2009 ◽  
Vol 81 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Jeff T. Williams ◽  
Edward J. Dick ◽  
John L. VandeBerg ◽  
Natalia E. Schlabritz-Loutsevitch ◽  
Rohina C. Rubicz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Macaca Fascicularis ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain

POLYMERASE CHAIN REACTION DETECTION AND SEROLOGIC FOLLOW-UP AFTER TREATMENT WITH BENZNIDAZOLE IN BOLIVIAN CHILDREN INFECTED WITH A NATURAL MIXTURE OF TRYPANOSOMA CRUZI I AND II

2006 ◽  
Vol 75 (3) ◽  
pp. 497-501 ◽  
Author(s):  
MARIA FLORES-CHAVEZ ◽  
JOSE-LOUIS ALCAZAR DALENZ ◽  
SIMONE FRÉDÉRIQUE BRENIÈRE ◽  
MARIE-FRANCE BOSSENO ◽  
MIREILLE HONTEBEYRIE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Natural Mixture ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction detection of cytomegalovirus genome in renal biopsies

1992 ◽  
Vol 42 (4) ◽  
pp. 1012-1016 ◽  
Author(s):  
Susan Kadereit ◽  
Susan Michelson ◽  
Beatrice Mougenot ◽  
Philippe Thibault ◽  
Pierre J. Verroust ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Renal Biopsies ◽  
Polymerase Chain

scholarly journals Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis

2017 ◽  
Vol 69 (6) ◽  
pp. 1443-1450 ◽  
Author(s):  
M.P. Campos ◽  
M.F. Madeira ◽  
D.A. Silva ◽  
M.S. Solcà ◽  
O.M. Espíndola ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Healthy Skin ◽  
Sample Collection ◽  
Chain Reaction ◽  
Dna Recovery ◽  
Ffpe Samples ◽  
Fresh Frozen ◽  
Polymerase Chain ◽  
Commercial Kits

ABSTRACT The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation study conducted with samples from 46 dogs from an endemic area in Brazil. After sample collection, DNA extractions were conducted using commercial kits and qPCR was oriented to kinetoplast DNA (kDNA) targets of the Leishmania infantum species. The results obtained for the FFPE samples showed 63.6% sensitivity and 77.1% specificity, whereas those obtained for the FT samples showed 100% and 48.6%, respectively. Poor agreement was observed for the results of the qPCR technique with FT and FFPE samples. Our results suggest freezing as the most suitable conservation method for the formation of sample databases considering DNA recovery

Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

2007 ◽  
Vol 42 (10) ◽  
pp. 1249-1255 ◽  
Author(s):  
Cibele dos Santos Ferrari ◽  
Luciana Lehmkuhl Valente ◽  
Fábio Cristiano Angonesi Brod ◽  
Caroline Tagliari ◽  
Ernani Sebastião Sant'Anna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Genetically Modified ◽  
Chain Reaction ◽  
Genetically Modified Soybean ◽  
Polymerase Chain

DNA Isolation from Recalcitrant Materials Such as Tree Roots, Bark, and Forest Soil for the Detection of Fungal Pathogens by Polymerase Chain Reaction

1998 ◽  
Vol 262 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Günther Bahnweg ◽  
Steffen Schulze ◽  
Evelyn M. Möller ◽  
Hilkea Rosenbrock ◽  
Christian Langebartels ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Forest Soil ◽  
Fungal Pathogens ◽  
Dna Isolation ◽  
Chain Reaction ◽  
Tree Roots ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction detection of sepsis-inducing pathogens in blood using SepsiTest™

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P10 ◽  
Author(s):  
Claudia Disqué ◽  
Anna-Julia Kochem ◽  
Helge Mühl ◽  
Michael Lorenz ◽  
Samir Sakka
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain
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