Production and characterization of monoclonal antibodies against Campylobacter fetus subsp. venerealis

Myeloma cells Sp2/0-Ag14 and spleen cells from BALB/c mouse immunized with sonicated Campylobacter fetus subsp. venerealis NCTC 10354 were fused with polyethylene glycol (PEG) for the selection of clones producing antibodies. Clones were obtained by limiting dilution and screened for the production of specific antibodies to C. fetus subsp. venerealis NCTC 10354 by indirect ELISA and western blot against a panel of bacteria: C. fetus subsp. venerealis NCTC 10354, C. fetus subsp fetus ADRI 1812, C. sputorum biovar sputorum LMG 6647, C. lari NCTC 11352, and Arcobacter skirrowii LMG 6621 for the ELISA and C. fetus subsp. venerealis NCTC 10354 and C. sputorum biovar sputorum LMG 6647 for the western blotting. Fifteen clones producing monoclonal antibodies (MAbs) anti-C. fetus subsp. venerealis of the IgM (1) and IgG (14) classes were further screened for species-specificity. Four clones of the 15 obtained were producers of species-specific monoclonal antibodies (MAbs): two were specific for C. fetus subsp. venerealis and two were specific for C. fetus subsp. fetus. None of the clones were reactive against C. sputorum biovar sputorum LMG 6647. All clones recognized a protein with molecular mass of approximately 148 kDa from lysed C. fetus subsp. venerealis NCTC 10354.

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Production and characterization of murine monoclonal antibodies against staphylococcal enterotoxins A and E

Canadian Journal of Microbiology ◽

10.1139/m91-098 ◽

1991 ◽

Vol 37 (8) ◽

pp. 581-585 ◽

Cited By ~ 1

Author(s):

Kunihiro Shinagawa ◽

Emiko Nishimura ◽

Makoto Mitsumori ◽

Naonori Matsusaka ◽

Shunji Sugii

Keyword(s):

Staphylococcus Aureus ◽

Monoclonal Antibodies ◽

The Other ◽

Staphylococcal Enterotoxin A ◽

Binding Assays ◽

Spleen Cells ◽

Myeloma Cells ◽

Murine Monoclonal Antibodies ◽

Competitive Binding Assays

Six murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin A (SEA) and enterotoxin E (SEE) were prepared by fusion of myeloma cells with mouse spleen cells immunized with SEA and SEE. Of five MAbs to SEA tested, two MAbs were reactive with only SEA, whereas three were specific for both SEA and SEE. On the other hand, one MAb to SEE was found to be specific for only SEE. To study specificities of the combining sites of these MAbs, competitive binding assays with either SEA or SEE and horseradish peroxidase conjugated MAbs were performed using unconjugated MAbs as inhibitors. The results obtained in the assays suggest that different epitopes may be located on SEA and that some of them may be cross-reacting epitopes between SEA and SEE. Key words: enterotoxins, monoclonal antibodies, Staphylococcus aureus.

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Production and characterization of a monoclonal antibody to biotin

Biochemical Journal ◽

10.1042/bj2370477 ◽

1986 ◽

Vol 237 (2) ◽

pp. 477-482 ◽

Cited By ~ 6

Author(s):

K Dakshinamurti ◽

R P Bhullar ◽

A Scoot ◽

E S Rector ◽

G Delespesse ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Myeloma Cell ◽

Enzyme Linked Immunosorbent Assay ◽

Spleen Cells ◽

Myeloma Cell Line ◽

Biotin Deficiency ◽

Keyhole Limpet Haemocyanin ◽

Limiting Dilution

Monoclonal antibodies to biotin have been prepared by using biotin linked to keyhole limpet haemocyanin (KLH) as the antigen. Spleen cells obtained from mice immunized with biotin-KLH were fused with the myeloma cell line NS-1. The resulting hybridomas were screened for the production of antibodies to biotin using an enzyme-linked immunosorbent assay. Clones producing antibodies to biotin were isolated by limiting dilution methods. Four cell lines, each derived originally from a different fusion, were chosen for the production of monoclonal antibodies. The monoclonal antibodies obtained have been characterized with respect to their ability to interact with biotin, biotin-bovine serum albumin, biotin-KLH and biocytin as well as to inhibit biotin-dependent enzymes. They have been used to produce cellular biotin deficiency in vitro for studies of biotin function.

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Production and characterization of monoclonal antibodies against Campylobacter fetus subsp. Venerealis

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2008.10.055 ◽

2009 ◽

Vol 128 (1-3) ◽

pp. 235

Author(s):

Telma Maria Alves ◽

Luiz Guilherme Dias Heneine ◽

Bárbara Silveira Araújo ◽

Luciana Maria Silva ◽

Patrícia Cota Campos ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Campylobacter Fetus ◽

Fetus Subsp

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Derivation of Sirococcusstrobilinus specific monoclonal antibodies

Canadian Journal of Forest Research ◽

10.1139/x86-166 ◽

1986 ◽

Vol 16 (5) ◽

pp. 939-944 ◽

Cited By ~ 7

Author(s):

Leslie Ann Mitchell

Keyword(s):

Monoclonal Antibodies ◽

Cell Lines ◽

Host Species ◽

Plant Tissues ◽

Spleen Cells ◽

Myeloma Cells ◽

Preliminary Characterization ◽

Shoot Blight ◽

Immunosorbent Assays

Cloned hydridoma cell lines producing monoclonal antibodies to Sirococcusstrobilinus (a seed-borne fungus causing shoot blight in pine, spruce, and fir seedlings) have been prepared by immunizing BALB/c mice with whole mycelium and fusing their spleen cells with SP2/0-Ag14 myeloma cells. Specificity for S. strobilinus was ascertained by testing the monoclonal antibodies in enzyme-linked immunosorbent assays and by indirect surface immunofluorescence tests against a panel of seven nonrelated commensal fungi obtained from the same seed lot as the Sirococcus isolate used in the derivation of the monoclonal antibodies. The monoclonal antibodies produced by these selected hydridoma cell lines also recognize antigens from other S. strobilinus isolates from diverse host species and tissues. The monoclonal reagents will thus be useful as diagnostic probes for locating S. strobilinus in or on seed and other plant tissues. Preliminary characterization of the morphologic distribution of antigens recognized by the monoclonal antibodies is discussed.

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Development of Neutralizing Monoclonal Antibodies for Oncogenic Human Papillomavirus Types 31, 33, 45, 52, and 58

Clinical and Vaccine Immunology ◽

10.1128/cvi.00773-13 ◽

2014 ◽

Vol 21 (4) ◽

pp. 587-593 ◽

Cited By ~ 16

Author(s):

Martha J. Brown ◽

Hanna Seitz ◽

Victoria Towne ◽

Martin Müller ◽

Adam C. Finnefrock

Keyword(s):

Monoclonal Antibodies ◽

Human Papillomavirus ◽

Immune Responses ◽

Neutralizing Antibodies ◽

Cervical Cancers ◽

Hpv Types ◽

Oncogenic Hpv ◽

Neutralizing Epitopes ◽

Selection Of

ABSTRACTHuman papillomavirus (HPV) is the etiological agent for all cervical cancers, a significant number of other anogenital cancers, and a growing number of head and neck cancers. Two licensed vaccines offer protection against the most prevalent oncogenic types, 16 and 18, responsible for approximately 70% of cervical cancer cases worldwide and one of these also offers protection against types 6 and 11, responsible for 90% of genital warts. The vaccines are comprised of recombinantly expressed major capsid proteins that self-assemble into virus-like particles (VLPs) and prevent infection by eliciting neutralizing antibodies. Adding the other frequently identified oncogenic types 31, 33, 45, 52, and 58 to a vaccine would increase the coverage against HPV-induced cancers to approximately 90%. We describe the generation and characterization of panels of monoclonal antibodies to these five additional oncogenic HPV types, and the selection of antibody pairs that were high affinity and type specific and recognized conformation-dependent neutralizing epitopes. Such characteristics make these antibodies useful tools for monitoring the production and potency of a prototype vaccine as well as monitoring vaccine-induced immune responses in the clinic.

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Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

Ciência Rural ◽

10.1590/0103-8478cr20200945 ◽

2021 ◽

Vol 51 (8) ◽

Author(s):

Eduardo Lara Ribeiro ◽

Patrícia Tiemi Fujimura ◽

Carlos Ueira-Vieira ◽

Luiz Ricardo Goulart ◽

Telma Maria Alves ◽

...

Keyword(s):

Phage Display ◽

Phage Library ◽

Hyperimmune Serum ◽

Subclinical Disease ◽

Campylobacter Fetus ◽

Fetus Subsp ◽

Cattle Herds ◽

Cervicovaginal Mucus ◽

Bovine Genital Campylobacteriosis

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

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Generation and Characterization of a Murine Monoclonal Antibody Specific for the Human T1-Cd5 Molecule

The International Journal of Biological Markers ◽

10.1177/172460088700200302 ◽

1987 ◽

Vol 2 (3) ◽

pp. 143-150 ◽

Cited By ~ 2

Author(s):

Federico Genzano ◽

Ada Funaro ◽

Massimo Alessio ◽

Lucia B. De Monte ◽

Graziella Bellone ◽

...

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

T Cell ◽

T Lymphocytes ◽

Membrane Glycoprotein ◽

Functional Features ◽

Specific Membrane ◽

Murine Monoclonal Antibodies ◽

Selection Of

Murine monoclonal antibodies (MoAbs) have found widespread applications in the characterization of the molecular and functional features of lymphocyte differentiation antigens. The present paper summarizes the results of our work dealing with the production and selection of a murine MoAb recognizing a molecule expressed during the whole differentiative life of T lymphocytes. The MoAb CB01 resulted to be specific for an apparently unique epitope of the T-cell specific membrane glycoprotein T1-CD5.

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Production and Characterization of Monoclonal Antibodies to Psittacine Beak and Feather Disease Virus

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063879200400104 ◽

1992 ◽

Vol 4 (1) ◽

pp. 13-18 ◽

Cited By ~ 6

Author(s):

Branson W. Ritchie ◽

Frank D. Niagro ◽

Kenneth S. Latimer ◽

W. L. Steffens ◽

Denise Pesti ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Myeloma Cell ◽

Enzyme Linked Immunosorbent Assay ◽

Spleen Cells ◽

Myeloma Cell Line ◽

Elisa System ◽

Mouse Myeloma Cell Line ◽

Feather Disease Virus ◽

Mouse Myeloma

Monoclonal antibodies specific for the virus that causes psittacine beak and feather disease (PBFD) were produced by fusing spleen cells from mice immunized with purified concentrated PBFD virus with mouse myeloma cell line Sp2/0. The resulting hybridomas were tested for reactivity against whole purified virus by an enzyme-linked immunosorbent assay (ELISA) system. Four clones, designated 15H8, 8E3, 11G12, and 2C3, were subcloned by limiting dilution. Isotyping indicated that clone 15H8 was secreting IgG, whereas the remaining clones secreted IgM. The secreted immunoglobulins were characterized by reactivity against purified PBFD virus using immunoblotting procedures, by immunohistochemical staining of virus-induced lesions in infected tissues, and by inhibition of PBFD virus agglutination of cockatoo erythrocytes. Antibodies secreted by clones 15H8 and 8E3 had the strongest activity against purified whole virus. Only immunoglobulin secreted by the clone 15H8 could be used to detect viral antigen in infected tissues. None of the monoclonal antibodies had hemagglutination-inhibition activity.

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