scholarly journals Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

2021 ◽  
Vol 51 (8) ◽  
Author(s):  
Eduardo Lara Ribeiro ◽  
Patrícia Tiemi Fujimura ◽  
Carlos Ueira-Vieira ◽  
Luiz Ricardo Goulart ◽  
Telma Maria Alves ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Library ◽  
Hyperimmune Serum ◽  
Subclinical Disease ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Cattle Herds ◽  
Cervicovaginal Mucus ◽  
Bovine Genital Campylobacteriosis

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

scholarly journals Prevalence of Tritrichomonas foetus and Campylobacter fetus subsp. venerealis among bulls slaughtered in the state of Minas Gerais, Brazil

2018 ◽  
Vol 39 (5) ◽  
pp. 2039
Author(s):  
Michele Placedino Andrade Botelho ◽  
Christian Hirsch ◽  
Andrey Pereira Lage ◽  
Christiane Maria Barcellos Magalhães da Rocha ◽  
Elaine Maria Seles Dorneles ◽  
...  
Keyword(s):  
Minas Gerais ◽  
Tritrichomonas Foetus ◽  
Sexually Transmitted ◽  
Molecular Tests ◽  
Campylobacter Fetus ◽  
Causative Agents ◽  
Fetus Subsp ◽  
Natural Mating ◽  
Cattle Herds ◽  
Bovine Genital Campylobacteriosis

Bovine genital campylobacteriosis (BGC) and bovine genital trichomonosis (BGT) are globally widespread, predominantly sexually transmitted diseases that mainly affect herds in which natural mating takes place. Bulls serve as good epidemiological indicators for studying the causative agents of these diseases in cattle herds. The objective of the present work was to determine the prevalence of BGC and BGT among bulls sent to slaughterhouses in southern Minas Gerais, Brazil. Samples of preputial smegma from 200 sexually mature, non-castrated bulls of several ancestries were collected in 2013 from four regional slaughterhouses. The polymerase chain reaction (PCR) was employed to detect Tritrichomonas foetus, Campylobacter fetus subsp. fetus, and C. fetus subsp. venerealis. Isolation also was used aiming to diagnosis of BGT. All smegma samples were negative in culture for T. foetus. Molecular tests revealed 8% (16/200) of animals to be positive for T. foetus, 17.5% (35/200) for Campylobacter. fetus subsp. fetus, and 13.5% (27/200) for C. fetus subsp. venerealis. The results of the present study indicate that T. foetus, C. fetus subsp. venerealis, and C. fetus subsp. fetus are present among bulls slaughtered in southern Minas Gerais, and that BGC and BGT occur in this region. These diseases therefore need to be considered during differential diagnosis of reproductive diseases affecting cattle herds in which natural mating is employed.

scholarly journals Bovine genital campylobacteriosis: main features and perspectives for diagnosis and control

2020 ◽  
Vol 50 (3) ◽  
Author(s):  
Cláudia Balzan ◽  
Rosangela Estel Ziech ◽  
Letícia Trevisan Gressler ◽  
Agueda Palmira Castagna de Vargas
Keyword(s):  
Asymptomatic Carrier ◽  
Venereal Disease ◽  
Molecular Techniques ◽  
Economic Losses ◽  
Biochemical Tests ◽  
Fetus Subsp ◽  
Natural Breeding ◽  
Cattle Herds ◽  
And Control ◽  
Bovine Genital Campylobacteriosis

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter fetus subsp. venerealis. In countries with large cattle herds, such as Brazil, where the use of natural breeding as a reproductive strategy is a common practice, BGC is considered an important cause of reproductive failure and economic losses. In these cases, the bull is the asymptomatic carrier of the bacterium and the infected females can have infertility and even abortions. The techniques for the diagnosis of C. fetus are isolation in culture medium and identification by biochemical tests, immunofluorescence, immunoenzymatic assays and molecular techniques. Disease control is based on vaccination with bacterins. This review described the epidemiology, etiology, pathogenesis, and advances in the diagnosis and control of BGC.

scholarly journals Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells

2016 ◽  
Vol 34 (4) ◽  
pp. 1419-1423
Author(s):  
María L Chiapparrone ◽  
Pedro Soto ◽  
María Catena
Keyword(s):  
Sperm Cells ◽  
Bovine Sperm ◽  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Genome Sequence of Campylobacter fetus subsp. venerealis NW_ED23, Isolated from Bovine Sheath Wash

2020 ◽  
Vol 9 (40) ◽  
Author(s):  
Mpinda Edoaurd Tshipamba ◽  
Stephen Abiola Akinola ◽  
Lubanza Ngoma ◽  
Mulunda Mwanza
Keyword(s):  
South Africa ◽  
Genome Sequence ◽  
Causative Agent ◽  
Content Type ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Bovine Genital Campylobacteriosis

ABSTRACT Campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis, which is mostly characterized by reproduction problems. The strain reported in this study was isolated from bull sheath wash in South Africa.

scholarly journals Production and characterization of monoclonal antibodies against Campylobacter fetus subsp. Venerealis

2009 ◽  
Vol 128 (1-3) ◽  
pp. 235
Author(s):  
Telma Maria Alves ◽  
Luiz Guilherme Dias Heneine ◽  
Bárbara Silveira Araújo ◽  
Luciana Maria Silva ◽  
Patrícia Cota Campos ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Production and characterization of monoclonal antibodies against Campylobacter fetus subsp. venerealis

2012 ◽  
Vol 32 (7) ◽  
pp. 640-644
Author(s):  
Telma M. Alves ◽  
Luiz G.D. Heneine ◽  
Bárbara S. Araújo ◽  
Luciana M. Silva ◽  
Patrícia C. Campos ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Indirect Elisa ◽  
Spleen Cells ◽  
Myeloma Cells ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Species Specific ◽  
Limiting Dilution ◽  
Selection Of

Myeloma cells Sp2/0-Ag14 and spleen cells from BALB/c mouse immunized with sonicated Campylobacter fetus subsp. venerealis NCTC 10354 were fused with polyethylene glycol (PEG) for the selection of clones producing antibodies. Clones were obtained by limiting dilution and screened for the production of specific antibodies to C. fetus subsp. venerealis NCTC 10354 by indirect ELISA and western blot against a panel of bacteria: C. fetus subsp. venerealis NCTC 10354, C. fetus subsp fetus ADRI 1812, C. sputorum biovar sputorum LMG 6647, C. lari NCTC 11352, and Arcobacter skirrowii LMG 6621 for the ELISA and C. fetus subsp. venerealis NCTC 10354 and C. sputorum biovar sputorum LMG 6647 for the western blotting. Fifteen clones producing monoclonal antibodies (MAbs) anti-C. fetus subsp. venerealis of the IgM (1) and IgG (14) classes were further screened for species-specificity. Four clones of the 15 obtained were producers of species-specific monoclonal antibodies (MAbs): two were specific for C. fetus subsp. venerealis and two were specific for C. fetus subsp. fetus. None of the clones were reactive against C. sputorum biovar sputorum LMG 6647. All clones recognized a protein with molecular mass of approximately 148 kDa from lysed C. fetus subsp. venerealis NCTC 10354.

scholarly journals Genomic and Phenotypic Characterization of Campylobacter fetus subsp. venerealis Strains

2021 ◽  
Vol 9 (2) ◽  
pp. 340
Author(s):  
Marta F. Silva ◽  
Ana L. Pereira ◽  
Maria J. Fraqueza ◽  
Gonçalo Pereira ◽  
Luísa Mateus ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Etiologic Agent ◽  
Phenotypic Characterization ◽  
Putative Virulence Factor ◽  
Whole Genome ◽  
Campylobacter Fetus ◽  
Virulence Potential ◽  
Fetus Subsp ◽  
Bovine Genital Campylobacteriosis

The pathogenesis mechanisms of Campylobacter fetus subsp. venerealis (Cfv), the etiologic agent of Bovine Genital Campylobacteriosis remain elusive. This study evaluated the virulence potential and biovar characteristics of Cfv isolates (n = 13) by PCR screening of putative virulence-factor (VF) genes, Multilocus Sequence Typing (MLST) analysis, antimicrobial susceptibility to tetracycline, penicillin, enrofloxacin and streptomycin testing and whole-genome sequencing (WGS; n = 5), also comparing the latter with 26 other whole-genome sequences of Cfv strains. The putative VF genes encoding type IV secretion system of Cfv (virB2-virB11/virD4) were absent in 92% of isolates, including isolates from aborted foetuses, evidencing that these VF genes are not essential for Cfv pathogenicity. The parA gene, used as a Cfv diagnostic molecular target, was detected in only 3 of 13 isolates, invalidating its use for diagnosis purposes. Three novel sequence types were identified by MLST. Although no in vitro antimicrobial resistance was detected, WGS identified antimicrobial resistance-related genes, including those encoding the multidrug efflux pumps CmeABC and YkkCD, indicating that their presence is not enough to provide antimicrobial resistance. The SNP and accessory protein families analysis segregated the Cfv and Cfv biovar intermedius (Cfvi) strains into different clusters. In conclusion, this study evidenced virulence potential and biovar characteristics of Cfv and Cfvi, which are of relevance for the control of Bovine Genital Campylobacteriosis.

scholarly journals Phage display demonstrates durable differences in serological profile by route of inoculation in primary infections of non-human primates with Dengue Virus 1

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jayant V. Rajan ◽  
Michael McCracken ◽  
Caleigh Mandel-Brehm ◽  
Greg Gromowski ◽  
Simon Pollett ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Phage Display ◽  
Denv Infection ◽  
Phage Library ◽  
Humoral Immune ◽  
Linear Peptide ◽  
Humoral Immune Responses ◽  
High Resolution Map ◽  
Domain Iii ◽  
Inoculation Route

AbstractNatural dengue virus (DENV) infections occur by mosquito bite but how the inoculation route affects the humoral immune response is unknown. We serologically profiled 20 non-human primates (NHP) from a prior study of DENV1 infection where animals were inoculated by mosquito (N = 10) or subcutaneous injection (N = 10). Using a comprehensive, densely tiled and highly redundant pan-flavivirus programmable phage library containing 91,562 overlapping 62 amino acid peptides, we produced a high-resolution map of linear peptide sequences enriched during DENV seroconversion. Profiles in mosquito-inoculated and subcutaneously-inoculated animals were similar up to 90 days after primary infection, but diverged at 1 year with differences in sero-reactivity in the Envelope (E; residues 215–406; p < 0.08), and Nonstructural-3 (NS3; residues 549–615; p < 0.05) proteins in mosquito-inoculated versus subcutaneously-inoculated animals. Within the E protein, residues 339–384 in domain III accounted for > 99% of the observed sero-reactivity difference. Antibody breadth did not vary by mode of inoculation. The differential reactivity to E domain III seen by phage display validated orthogonally by ELISA, but did not correlate with late neutralization titers. Serological profiling of humoral immune responses to DENV infection in NHP by programmable phage display demonstrated durable differences in sero-reactivity by route of inoculation.

scholarly journals Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1225
Author(s):  
Jiawen Cao ◽  
Tiantian Fan ◽  
Yanlian Li ◽  
Zhiyan Du ◽  
Lin Chen ◽  
...  
Keyword(s):  
Phage Display ◽  
Cyclic Peptide ◽  
Protein Complexes ◽  
Peptide Ligands ◽  
Phage Library ◽  
Cancer Drug ◽  
Protein Protein Interaction ◽  
Phage Display Technique ◽  
Peptide Mimic ◽  
Human Proteins

WD40 is a ubiquitous domain presented in at least 361 human proteins and acts as scaffold to form protein complexes. Among them, WDR5 protein is an important mediator in several protein complexes to exert its functions in histone modification and chromatin remodeling. Therefore, it was considered as a promising epigenetic target involving in anti-cancer drug development. In view of the protein–protein interaction nature of WDR5, we initialized a campaign to discover new peptide-mimic inhibitors of WDR5. In current study, we utilized the phage display technique and screened with a disulfide-based cyclic peptide phage library. Five rounds of biopanning were performed and isolated clones were sequenced. By analyzing the sequences, total five peptides were synthesized for binding assay. The four peptides are shown to have the moderate binding affinity. Finally, the detailed binding interactions were revealed by solving a WDR5-peptide cocrystal structure.

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