scholarly journals Does troncular vagotomy modify the proliferative gastric lesions induced in rats by duodenogastric reflux?

2007 ◽  
Vol 22 (3) ◽  
pp. 210-214 ◽  
Author(s):  
Paulo Antônio Rodrigues ◽  
Shoiti Kobayasi ◽  
Maria Aparecida Marchesan Rodrigues
Keyword(s):  
Gastric Mucosa ◽  
Gastric Wall ◽  
Duodenogastric Reflux ◽  
Biological Behavior ◽  
Jejunal Loop ◽  
Antral Mucosa ◽  
Benign Lesions ◽  
Pyloric Mucosa ◽  
Male Wistar Rats ◽  
Anterior Gastric Wall

PURPOSE: to investigate if combining VT to DGR through the pylorus can modulate the biological behavior of PL induced by DGR and to verify if TV alone can induce morphologic lesions in the gastric mucosa. METHODS: 62 male Wistar rats were assigned to four groups: 1 - Control (CT) gastrotomy; 2 - Troncular Vagotomy (TV) plus gastrotomy; 3 - Duodenogastric reflux through the pylorus (R) and 4 - Troncular vagotomy plus DGR (RTV). The animals were killed at the 54 week of the experiment. DGR was obtained by anastomosing a proximal jejunal loop to the anterior gastric wall. TV was performed through isolation and division of the vagal trunks. Gastrotomy consisted of 1 cm incision at the anterior gastric wall. PL were analyzed gross and histologically in the antral mucosa, at the gastrojejunal stoma and at the squamous portion of the gastric mucosa. RESULTS: Groups R and RTV developed exophytic lesions in the antral mucosa (R=90.9%; RTV=100%) and at the gastrojejunal stoma (R=54.54%; RTV=63.63%). Histologically they consisted of proliferative benign lesions, without cellular atypias, diagnosed as adenomatous hyperplasia. Both groups exposed to DGR presented squamous hyperplasia at the squamous portion of the gastric mucosa (R= 54.5%; RTV= 45.4%). TV, alone, did not induce gross or histological alterations in the gastric mucosa. TV did note change the morphologic pattern of the proliferative lesions induced by DGR. CONCLUSIONS: DGR induces the development of PL in the pyloric mucosa and at the gastrojejunal stoma. TV does not change the morphologic pattern of the proliferative lesions induced by DGR. TV alone is not able to induce morphologic lesions in the gastric mucosa.

scholarly journals Influence of duodenogastric reflux in the gastric mucosa histological changes of rats infected with Helicobacter pylori

2016 ◽  
Vol 43 (4) ◽  
pp. 235-242 ◽  
Author(s):  
JOSÉ CARLOS RIBEIRO DE ARAUJO ◽  
JORGE JOSÉ DE CARVALHO ◽  
HUMBERTO OLIVEIRA SERRA
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Chronic Gastritis ◽  
Partial Gastrectomy ◽  
Duodenogastric Reflux ◽  
Histological Changes ◽  
Positive Correlation ◽  
Male Wistar Rats ◽  
H Pylori ◽  
The One

ABSTRACT Objective: to evaluate the influence of Duodenal reflux in histological changes of the gastric mucosa of rats infected with Helicobacter pylori submitted to pyloroplasty. Methods: after two weeks of acclimation, we infected 30 male Wistar rats with Helicobacter pylori. We randomly divided them into three groups: one submitted to pyloroplasty, another to partial gastrectomy and the third, only infected, was not operated. After six months of surgery, euthanasia was carried out. Gastric fragments were studied by light microscopy to count the number of H. pylori, and to observe the histological changes (gastritis, metaplasia, dysplasia and neoplasia). We confirmed these changes by immunohistochemistry using the molecular markers PCNA and TGF-beta. Results: the animals submitted to pyloroplasty had higher percentage of colonization by H. pylori (median=58.5; gastrectomy=16.5; control=14.5). There was a positive correlation between the amount of H. pylori and the occurrence of chronic gastritis present in the antral fragments. Neoplasia occurred in 40% of rats from the group submitted to pyloroplasty. The staining with PCNA and TGF-ß confirmed the histopathological changes visualized by optical microscopy. Conclusions: the antral region was the one with the highest concentration of H. pylori, regardless of the group. There was a positive correlation between the appearance of benign disorders (chronic gastritis, metaplasia, dysplasia) and cancer in mice infected with H. pylori submitted to pyloroplasty.

“LAPAROSCOPIC INTRAGASTRIC EXCISION OF POSTERIORLY LOCATED GASTRIC GIST USING CONVENTIONAL LAPAROSCOPIC INSTRUMENTS – A CASE REPORT”

2021 ◽  
pp. 29-31
Author(s):  
Kulwant Singh Bhau ◽  
Iqbal Saleem Mir ◽  
Mufti Mahmood Ahmad
Keyword(s):  
Gastric Wall ◽  
Surgical Excision ◽  
Anterior Wall ◽  
Minimal Invasive ◽  
Point Of View ◽  
Gastric Gist ◽  
Safe Procedure ◽  
Conventional Laparoscopic ◽  
Laparoscopic Instruments ◽  
Anterior Gastric Wall

Background: Gastro-intestinal stromal tumour (GIST) commonly involves stomach. Recently there has been an inclination towards managing these benign but potentially malignant lesions by minimal invasive techniques. Surgical excision of gastric GIST mostly requires anterior wall gastrostomy especially for intraluminal lesions. The size and location of the lesion are critical from technical point of view. Lesions located at gastro-esophageal junction requires larger anterior gastric wall opening to reach the site of tumour for excision. Endoscopic excision for such lesions is not always amenable. We performed excision of a posteriorly locat Methods: ed gastric GIST at GE junction by hitching the anterior gastric wall with the anterior abdominal wall and by directly creating pneumogastrium percutaneously for placing three intra-gastric trocars. Results: Patient was discharged on post-operative day 3 in a satisfactory condition. Histopathology revealed complete resection of GIST lesion with margins free from tumour. Immuno-histochemistry (IHC) conrmed it to be GIST with low malignant potential and patient was advised regular follow up. Laparoscopic intra-gastric excision of a posteriorly located gast Conclusion: ro-oesophageal junction GIST lesion after creating pneumogastrium and using conventional laparoscopic instruments is a safe procedure

scholarly journals Features of the endoscopic picture in paediatric gastroduodenal diseases caused by Helicobacter pylori

2020 ◽  
Vol 10 (3) ◽  
pp. 543-550
Author(s):  
Sh. T. Turdieva ◽  
E. A. Shamansurova
Keyword(s):  
Helicobacter Pylori ◽  
Inflammatory Diseases ◽  
Gastric Wall ◽  
Duodenogastric Reflux ◽  
Chronic Atrophic Gastritis ◽  
Endoscopic Examination ◽  
Ulcer Disease ◽  
Functional Studies ◽  
H Pylori ◽  
Endoscopic Picture

The aim of the study was to examine the features of the endoscopic picture in the upper digestive tract mucosa in paediatric chronic gastroduodenal pathology (CGDP) associated with Helicobacter pylori. Materials and methods. There were examined 286 patients, aged 6–15 years. Diagnostic criteria for chronic gastroduodenal pathology were anamnestic as well as instrumental and functional studies data: gastric fractional intubation, esophagogastroduodenoscopy (EPGDS) with endoscopic pH-metry without biopsy, and ultrasound examination of abdominal organs. H. pylori testing was carried out by two unrelated methods such as respiratory test and a immunochromatographic fecal test. Results. Detection of H. pylori in children with CGDP peaked in patients with peptic gastric and duodenal ulcer (up to 87.5%, p < 0.05). The main endoscopic signs were edema, hyperaemia and contact bleeding, as well as local haemorrhage were the major endoscopic signs of inflammation in the stomach and duodenum mucosa. Atrophic mucosal lesions were characterized by thinning, pale colour together with transilluminated submucosal vessels. Non-atrophic antral gastritis was featured with delayed gastric emptying, antral stasis and pyloric spasm. In contrast, hypotension of the gastric wall, duodenogastric reflux and decreased motility were more typical to chronic atrophic gastritis. Major endoscopic feature in patients with H. pylori infection was presented by dominant atrophic changes combined with gastroduodenal reflux (77.6%, p < 0.05) compared to patients without H. pylori infection. Conclusion. Detection of HP infection was peaked in children with CGDP coupled to peptic ulcer disease compared to patients with inflammatory diseases (p < 0.05). Endoscopic examination in HP-positive patients showed that atrophic changes were found by 4-fold more frequently together with gastroduodenal reflux compared to patients without HP infection (p < 0.05).

Comparison of Anterior Gastric Wall and Greater Gastric Curvature Invaginations for Weight Loss in Rats

2007 ◽  
Vol 17 (10) ◽  
pp. 1340-1345 ◽  
Author(s):  
Pedro E. B. Fusco ◽  
Renato S. Poggetti ◽  
Riad N. Younes ◽  
Belchor Fontes ◽  
Dario Birolini
Keyword(s):  
Weight Loss ◽  
Gastric Wall ◽  
Anterior Gastric Wall

scholarly journals Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882) Baylis, 1920 in rabbits

2004 ◽  
Vol 56 (3) ◽  
pp. 325-332 ◽  
Author(s):  
L.A. Barros ◽  
R. Tortelly ◽  
R.M. Pinto ◽  
D.C. Gomes
Keyword(s):  
Gastric Mucosa ◽  
Gastric Wall ◽  
Clinical Signs ◽  
Nematode Species ◽  
Mammalian Host ◽  
Post Inoculation ◽  
Bright Field ◽  
Experimental Inoculation ◽  
Bright Field Microscopy ◽  
Eustrongylides Ignotus

Rabbits were infected per os with 10 Eustrongylides ignotus L4 and with 50 Contracaecum multipapillatum L3 per rabbit, recovered from naturally infected freshwater fishes (Hoplias malabaricus) in order to evaluate the patogenicity of these two nematode species in mammalian host. Two rabbits (20%) infected with E. ignotus died before the fourth day post-inoculation (one after 51 and the other after 78 hours). Six rabbits (60%) were inappetent until the fifth day following experimental inoculation. No clinical signs in rabbits inoculated with C. multipapillatum were observed; nevertheless, eight (80%) animals were positive for this nematode species. Rabbits inoculated with E. ignotus, had gastric congestion with hematoma of the gastric wall in 60% of the cases. Peritoneum was congested in 20% of the animals with the presence of peritoneal abscess in 10% of the cases. All inoculated animals showed hyperemia of the gastric mucosa with hemorrhagic gastritis due to infections with E. ignotus. In C. multipapillatum inoculated animals, the hyperemia was followed by disruption of the epithelial mucosa in the sites of parasite attachment. In the gastric mucosa, miscellaneous leukocitary infiltrates, with multifocal necrosis reaching the submucosa in the infections with C. multipapillatum were observed under bright field microscopy. Perforating lesions in several organs, mainly in the gastric wall, pancreas and liver, always in the presence of a mixed inflammatory process, intensely fibrous, with hemorrhage and necrosis were observed in animals infected with E. ignotus.

scholarly journals SURGICAL GASTROSTOMY BASED ON ENDOSCOPIC CONCEPTS

2016 ◽  
Vol 29 (1) ◽  
pp. 50-52
Author(s):  
Emmanuel Conrado SOUZA
Keyword(s):  
Gastric Wall ◽  
Percutaneous Approach ◽  
Endoscopic Gastrostomy ◽  
Minimal Incision ◽  
Left Hypochondrium ◽  
Direct View ◽  
Anterior Gastric Wall ◽  
Enteral Access ◽  
Surgical Gastrostomy

Background: Until the early 1980s, Stamm technique was considered standard method to gastrostomy. After description of the endoscopic technique, due to its efficiency and speed, quickly became the method of choice for long-term enteral access. Aim: Describe a technique that combines direct view of the stomach from open surgery with the simplicity and less traumatic endoscopic gastrostomy method. Method: In patient supine under spinal anesthesia the technique stars with small epigastric incision to pull up the stomach. A 3 mm incision in the left hypochondrium is made to pass needle puncture to guidewire passage. The stomach is drilled, guidewire is seizured, connection to catheter and percutaneous approach is made with traction of the stomach to the abdominal wall. Purse suture on the anterior gastric wall is not needed. Results: Twenty-eight patients underwent gastrostomy using endoscopy devices; six had local minor complications without the need for re-intervention; there was no death. Conclusion: The surgical gastrostomy with minimal incision in the stomach to pull off the catheter using endoscopic gastrostomy devices, proved to be safe, easy to perform, less traumatic, quick, simple and elegant.

Perplexing Gastrointestinal Pathology: A Case Report on Ectopic Gastric Tissue Presenting as Rectal Mass

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S70-S70
Author(s):  
Suhalika Sahni ◽  
Vikas Mehta
Keyword(s):  
Inflammatory Bowel Disease ◽  
Gastric Mucosa ◽  
Bowel Disease ◽  
Clinical Signs ◽  
Lower Abdominal Pain ◽  
Antral Mucosa ◽  
Gastric Tissue ◽  
Gastric Heterotopia ◽  
Rectal Mass ◽  
Inflammatory Bowel

Abstract Objectives Gastric heterotopia (GHT) is a commonly reported epithelial heterotopia and has been described as heteroplasia (congenital) during organogenesis or metaplasia (acquired) during process of damaged epithelial repair. GHT can occur along the GI tract from nose to anus; it is predominantly seen in esophagus, duodenum, and Meckel’s diverticulum. It is the most common subtype of epithelial heterotopia. However, the presence of gastric mucosa in the rectum is a very uncommon entity. The endoscopic prevalence of GHT can reach up to 11% in foregut and midgut with a lesser prevalence in hindgut. Methods We present an interesting case of heterotopic gastric mucosa presenting as a rectal mass. A 27-year-old female patient presented with lower abdominal pain and episodic bloody diarrhea. After initial workup, a colonoscopy was done, which showed a 3-cm mass in the rectum. Features of inflammatory bowel disease were not identified. Results At the first rectal biopsy, multiple fragments of gastric mucosa, with some minimally attached rectal crypts, were identified and a diagnosis of gastric heterotopia was made. Subsequently, the mass was resected and similar findings were identified, confirming the diagnosis. Our case showed mixed oxyntic and antral mucosa comingling with rectal-type mucosa. Immunohistochemical studies were performed, which showed CDX2 and CK20 being positive in rectal and CK7 in gastric mucosa. Conclusion Gastric heterotopia is a rare entity that can present as a solitary lesion. The clinical signs and symptoms can mimic those of inflammatory bowel disease. Floater or contamination is an important pitfall in this entity and can lead to an erroneously diagnose. Due to high frequency of having floater in some histology laboratories, we recommend ordering multiple deeper recuts on cases when the pathologist sees closeness or attachment of gastric tissue to the rectal tissue.

Effect of histamine on canine gastric mucosal adenylate cyclase.

1977 ◽  
Vol 232 (1) ◽  
pp. E35
Author(s):  
R R Dozois ◽  
A Wollin ◽  
R D Rettmann ◽  
T P Dousa
Keyword(s):  
Gastric Mucosa ◽  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Antral Mucosa ◽  
Fundic Mucosa ◽  
H2 Receptors ◽  
Dose Dependent ◽  
Stimulation Of

The effects of histamine, Nalpha-dimethylhistamine, 4,5-methylhistamine, Ntau-methylhistamine, pentagastrin, carbachol, and NaF on the adenylate cyclase activity from canine gastric mucosa were investigated in cell-free preparations. In gastric fundic mucosa, histamine (10(-4) M), Nalpha-dimethylhistamine (10(-4) M), 4,5-methylhistamine (10(-4 M), and NaF (10)-2) M) significantly (P less than 0.001) increased adenylate cyclase activity (means+/-SE) by 44.7+/-6.6, 49.4+/-6.7, 34.0+/-6.4, and 572.0+/-100%, respectively, above basal activity. The effect of histamine and Na-dimethyl histamine was dose-dependent. In contrast, other tested agents failed to stimulate the formation of cyclic AMP in gastric fundic mucosa. Metiamide (10(-4) M) blocked the stimulation of fundic mucosa adenylate cyclase by histamine and Nalpha-dimethylhistamine, without significantly altering basal and NaF-induced adenylate cyclase activity. Histamine, however, did not stimulate the adenylate cyclase activity from the gastric antral mucosa. The findings support the proposal that the canine gastric acid response to histamine may be mediated by cyclic AMP formed in response to stimulation of histamine H2-receptors.

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