scholarly journals Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

2003 ◽  
Vol 60 (4) ◽  
pp. 683-689 ◽  
Author(s):  
Mariza Monteiro ◽  
Beatriz Appezzato-da-Glória ◽  
Maria José Valarini ◽  
Carlos Alberto de Oliveira ◽  
Maria Lucia Carneiro Vieira
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Medicago Sativa ◽  
Cell Suspension ◽  
High Efficiency ◽  
Point Of View ◽  
Ability To Regenerate

Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.

scholarly journals Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

2008 ◽  
Vol 13 (4) ◽  
pp. 363 ◽  
Author(s):  
R. MA ◽  
S. PULLI
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Developmental Stage ◽  
Embryogenic Callus ◽  
Immature Embryo ◽  
Green Plant ◽  
Somatic Tissue ◽  
Winter Rye ◽  
Effi Ciency

Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a total 27 rye genotypes including 10 spring and 17 winter genotypes were involved in the investigation. Genotype, culture medium, sugar, gel agent and auxin infl uenced somatic embryogenesis of immature embryo signifi cantly. One-two weeks cold pretreatment of young embryo enhanced somatic embryogenesis and green plant regeneration. In culture of immature embryos, infl orescences and leaf segments of the seedlings, explants signifi cantly infl uenced the culture effi ciency. Highest embryogenic callus yield resulted from rye immature embryo as explant compared to young infl orescence and leaf segment of seedling. Developmental stage of embryo played an important role in somatic embryogenesis. Late spherical coleoptile stage (embryo size 0.5–1mm in length) was optimal developmental stage of immature embryo for culture. Morphogenetic potential of embryogenic callus decreased with an increasing number of subcultures, and this ability could be maintained in vitro for a maximum of 8 months of culturing.;

scholarly journals Cytomorphological studies on somatic embryogenesis of Gentiana tibetica (King) and G. cruciata (L.)

2014 ◽  
Vol 65 (1-2) ◽  
pp. 47-51 ◽  
Author(s):  
Anna Mikuła ◽  
Maria Wesołowska ◽  
Józef Kapusta ◽  
Lutosława Skrzypczak ◽  
Jan J. Rybczyński
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Agar Medium ◽  
Induction Medium ◽  
Mineral Salts ◽  
Fast Green ◽  
Seedling Explants ◽  
Callus Initiation ◽  
Pas Reaction

The process of plant regeneration via somatic embryogenesis of two gentianas, <em>Gentiana tibetica</em> and <em>G. cruciata</em> was described. For this purpose seedling explants were cultured on agar medium and later maintained in cell suspension. For callus initiation seedling explants like: cotyledons, hypocotyl and root were plated on a callus induction medium (CIM) composed of MS (1962), supplemented with 0.5 mg/l 2,4-D and 1.0 mg/l Kin. For the formation of cell suspension culture, embryogenic callus was transferred into liquid maintained medium (MM) composed of MS (1962), supplemented with 1.0 mg/l Dic + 0.1 mg/l NAA + 2.0 mg/l BAP + 80.0 mg/l SA. The conversion of somatic embryo into plantlets required a new medium (ECM) based on MS (1962) mineral salts, supplemented with 0.5 mg/l GA<sub>3</sub> + 1.0 mg/l Kin + 0.5 mg/l NAA. For cytomorphological studies of particular stages of embryogenesis, specimens were stained with dyes and reagents: 1. PAS reaction with leukofucsin, 2. Safranin + fast green, 3. Erlich's hematoxylin.

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