Titration of serum anti-ganglioside antibodies in patients with chronic medular injury previous to treatment with GM1 ganglioside

Anti-ganglioside serum titers were evaluated by ELISA in 150 patients with complete spinal cord lesion for 6 to 12 months (IgG monosialo GM1, IgM monosialo GM1, IgG asialo GM1, IgM asialo GM1, IgG disialo GD1b e IgM disialo GD1b) prior to treatment with GM1 100 mg/day i.m. Only 4 patients showed positive titers for anti-asialo-GM1 (IgM) antibodies . All patients were clinically examined during and after treatment. No important side effects were observed with GM1 therapy. These results suggest that GM1-ganglioside administration in patients with chronic spinal cord injury is safe.

Download Full-text

Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00625.2011 ◽

2012 ◽

Vol 302 (7) ◽

pp. E864-E871 ◽

Cited By ~ 18

Author(s):

Hanneke Boon ◽

Emil Kostovski ◽

Sergej Pirkmajer ◽

Moshi Song ◽

Irina Lubarski ◽

...

Keyword(s):

Physical Activity ◽

Skeletal Muscle ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Regulatory Protein ◽

Spinal Cord Lesion ◽

Regulatory Proteins ◽

Control Group ◽

Cord Injury ◽

Cord Lesion

Na+-K+-ATPase is an integral membrane protein crucial for the maintenance of ion homeostasis and skeletal muscle contractibility. Skeletal muscle Na+-K+-ATPase content displays remarkable plasticity in response to long-term increase in physiological demand, such as exercise training. However, the adaptations in Na+-K+-ATPase function in response to a suddenly decreased and/or habitually low level of physical activity, especially after a spinal cord injury (SCI), are incompletely known. We tested the hypothesis that skeletal muscle content of Na+-K+-ATPase and the associated regulatory proteins from the FXYD family is altered in SCI patients in a manner dependent on the severity of the spinal cord lesion and postinjury level of physical activity. Three different groups were studied: 1) six subjects with chronic complete cervical SCI, 2) seven subjects with acute, complete cervical SCI, and 3) six subjects with acute, incomplete cervical SCI. The individuals in groups 2 and 3 were studied at months 1, 3, and 12 postinjury, whereas individuals with chronic SCI were compared with an able-bodied control group. Chronic complete SCI was associated with a marked decrease in [3H]ouabain binding site concentration in skeletal muscle as well as reduced protein content of the α1-, α2-, and β1-subunit of the Na+-K+-ATPase. In line with this finding, expression of the Na+-K+-ATPase α1- and α2-subunits progressively decreased during the first year after complete but not after incomplete SCI. The expression of the regulatory protein phospholemman (PLM or FXYD1) was attenuated after complete, but not incomplete, cervical SCI. In contrast, FXYD5 was substantially upregulated in patients with complete SCI. In conclusion, the severity of the spinal cord lesion and the level of postinjury physical activity in patients with SCI are important factors controlling the expression of Na+-K+-ATPase and its regulatory proteins PLM and FXYD5.

Download Full-text

Chronic pain-related syndrome in rats after ischemic spinal cord lesion: a possible animal model for pain in patients with spinal cord injury

Pain ◽

10.1016/0304-3959(92)90070-r ◽

1992 ◽

Vol 48 (2) ◽

pp. 279-290 ◽

Cited By ~ 155

Author(s):

X.-J. Xu ◽

J.-X. Hao ◽

H. Aldskogius ◽

Å. Seiger ◽

Z. Wiesenfeld-Hallin

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Chronic Pain ◽

Animal Model ◽

Spinal Cord Lesion ◽

Cord Injury ◽

Cord Lesion

Download Full-text

Has GM1 ganglioside been shown to be effective in the restoration of neurologic function in persons with chronic spinal cord injury? A critique of an article by Judith B. Walker and Michelle Harris

Neuroscience Letters ◽

10.1016/0304-3940(94)90100-7 ◽

1994 ◽

Vol 176 (2) ◽

pp. 277-280 ◽

Cited By ~ 3

Author(s):

K.John Klose ◽

Blair Calancie

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Gm1 Ganglioside ◽

Chronic Spinal Cord Injury ◽

Cord Injury ◽

Neurologic Function

Download Full-text

Faculty Opinions recommendation of Effects of transplantation of olfactory ensheathing cells in chronic spinal cord injury: a systematic review and meta-analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718484796.793518258 ◽

2016 ◽

Author(s):

Michael Fehlings ◽

Christopher Ahuja ◽

Allan Martin

Keyword(s):

Systematic Review ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Meta Analysis ◽

Olfactory Ensheathing Cells ◽

Chronic Spinal Cord Injury ◽

Cord Injury ◽

Ensheathing Cells

Download Full-text

Effect of Teriparatide, Vibration and the Combination on Bone Mass and Bone Architecture in Chronic Spinal Cord Injury

10.21236/ada599169 ◽

2013 ◽

Author(s):

Thomas J. Schnitzer

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Bone Mass ◽

Bone Architecture ◽

Chronic Spinal Cord Injury ◽

Cord Injury

Download Full-text

Pharmacological Approaches to Chronic Spinal Cord Injury

Current Pharmaceutical Design ◽

10.2174/1381612811319240009 ◽

2013 ◽

Vol 19 (24) ◽

pp. 4423-4436 ◽

Cited By ~ 10

Author(s):

Inge Steuer ◽

Pascal Rouleau ◽

Pierre Guertin

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Chronic Spinal Cord Injury ◽

Cord Injury

Download Full-text

Polyphenols as Potential Therapeutics for Pain and Inflammation in Spinal Cord Injury

Current Molecular Pharmacology ◽

10.2174/1874467213666201223111743 ◽

2020 ◽

Vol 13 ◽

Author(s):

Ashif Iqubal ◽

Musheer Ahmed ◽

Mohammad Kashif Iqubal ◽

Faheem Hyder Pottoo ◽

Syed Ehtaishamul Haque

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Side Effects ◽

Transient Receptor Potential ◽

Receptor Potential ◽

Neutrophil Infiltration ◽

Matrix Metal ◽

Mediators Of Inflammation ◽

Major Player ◽

Cord Injury

: Spinal cord injury (SCI) and associated pain and inflammation caused by the trauma or infection is one of the serious health care issues world-wide. The various inflammatory, redox-sensitive and apoptotic events are contributing factor but altered neuronal function, axonal degeneration, activated microglia, endothelial cells, astrocytes, fibroblasts,pericytes, Schwann cells, meningeal cells are the major player in its pathogenesis. Further, monocytes and neutrophil infiltration get recruited and facilitate the release of chemokines, cytokines, and other mediators of inflammation. This event leads to the production of different amino acids, neuropeptides kinin, prostaglandins, prostacyclin, thromboxane, leukotrienes, bradykinin, histamine, matrix metal proteinases and serotonin that stimulate nerve endings and manifests the inflammation and pain processes, etc. Arachidonic acid (AA), NF-kB, NLRP3 inflammasome, and nitric oxide pathways along with P2X7 receptor and ion channel transient receptor potential (TRP) vanilloid are some of the recently explored targets for modulation of pain and inflammation in SCI. Till now, NSAIDs, opioids, antidepressants, anticonvulsants, NMDA antagonists, α2-adrenergic agonists, and GABA-receptor agonists are used for the management of these pathological conditions. However, these drugs are associated with various side effects. Additionally, the number of available animal models for SCI has enhanced the understanding of the complex pathological mechanisms involved in the generation of chronic inflammatory pain in SCI. These findings enable us to identify and validate several potent natural analgesic-anti-inflammatory drug candidates with minimal side effects. However, until now, these compounds have been studied in preclinical models and shown promising results but no clinical studies have been performed. Therefore, a detailed exploration of these natural compounds is important for bringing them from bench to bedside.

Download Full-text