scholarly journals Morphological dimorphism in the Y chromosome of "pé-duro" cattle in the Brazilian State of Piauí

1999 ◽  
Vol 22 (3) ◽  
pp. 369-373 ◽  
Author(s):  
Carmen M.C. Britto ◽  
Maria Luiza S. Mello

"Pé-duro" (hard foot) is a rare breed of beef cattle of European (Bos taurus taurus) origin, originated in northern and northeastern Brazil. Y chromosome morphology, outer genital elements and other phenotypic characteristics were examined in 75 "pé-duro" bulls from the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) herd in the Brazilian State of Piauí. The purpose was to investigate possible racial contamination with Zebu animals (Bos taurus indicus) in a cattle that has been considered closest to its European origin (B. t. taurus). The presence of both submetacentric and acrocentric Y chromosomes, typical of B. t. taurus and B. t. indicus, respectively, and the larger preputial sheath in bulls with an acrocentric Y chromosome indicated racial contamination of the "pé-duro" herd with Zebu cattle. Phenotypic parameters involving horn, dewlap, ear, chamfer, and coat color characteristics, indicative of apparent racial contamination, were not associated with acrocentric Y chromosome.

2006 ◽  
Vol 41 (11) ◽  
pp. 1609-1615 ◽  
Author(s):  
Érica Cunha Issa ◽  
Wilham Jorge ◽  
José Robson Bezerra Sereno

The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin, indicating that the participation of zebuines in the formation of the breed occurred entirely through the paternal line.


Zootaxa ◽  
2009 ◽  
Vol 2227 (1) ◽  
pp. 1-31 ◽  
Author(s):  
S. C. DONNELLAN ◽  
P. J. COUPER ◽  
K. M. SAINT ◽  
L. WHEATON

Using a combination of mitochondrial and nuclear genetic markers, karyotypes and morphology, we examine the taxonomy of the Australo-papuan scincid lizard Carlia ‘fusca’ complex in northern Australia, all of which had been assigned previously to C. longipes. Carlia longipes, shows substantial variation in Y chromosome morphology between populations, indeed more than is seen between other species of Carlia. Analyses of the molecular genetic data and morphology demonstrate that populations with different Y chromosomes are two different species and also lead to the recognition of a third species from the Torres Strait. We herein define each of these species, for which previously described names can be applied.


2008 ◽  
Vol 31 (2) ◽  
pp. 468-474 ◽  
Author(s):  
Severino B.P. Barbosa ◽  
Ricardo P. Ramalho ◽  
Humberto G. Monardes ◽  
Flávio M. Dias ◽  
Djalma C. dos Santos ◽  
...  

1997 ◽  
Vol 20 (1) ◽  
pp. 93-96 ◽  
Author(s):  
Ana A.L. Barbosa ◽  
Iglenir J. Cavalli ◽  
Kiyoko Abé ◽  
Maria G. Santos ◽  
Eliane S. Azevêdo

The variability of the lengths of the heterochromatic and euchromatic segments of the human Y chromosome was studied by a quantitative method of densitometric measurement in 60 normal and unrelated black individuals (30 with and 30 without devotional surnames), living in Salvador, Bahia, northeastern Brazil. Thirty normal and unrelated Caucasian individuals of European origin, living in Curitiba, Paraná, south Brazil, were included as controls. The heterochromatic segment and total Y chromosome lengths were greater in caucasians than in blacks without devotional surnames, and these were greater than in blacks with devotional surnames. These findings are in agreement with previous reports of a higher percentage of black ancestry in blacks carrying devotional surnames than those carrying non-devotional ones.


2019 ◽  
Vol 13 (3) ◽  
pp. 265-275
Author(s):  
Andréia Pires Amancio ◽  
Sabrina Sara Moreira Duarte ◽  
Rafael Carneiro Silvafael ◽  
Alex Silva da Cruz ◽  
Danilo Conrado Silva ◽  
...  

Chromosome banding techniques were applied and standardized to obtain karyotype characteristics for the first time in Brazil of Nelore cattle – Bos taurus indicus Linnaeus, 1758 – (bovine subspecies most prominent in Brazilian livestock). Blood samples were collected from the animals of the School of Agrarian and Biological Sciences of the Pontifical Catholic University of Goiás, two males and two females of pure breed. These samples were submitted to the cell culture method to study metaphase chromosomes. Chromosome banding techniques (C, G and NOR) revealed the karyotype architecture of Nelore cattle common with that of other breeds of zebu cattle formerly karyotyped. The diploid chromosome number was invariably normal, 2n = 60. C-banding revealed C-positive heterochromatin in centromeric regions almost in all chromosomes. G-banding presented the expected band pattern in the respective chromosome pairs in correspondence with the established chromosomal patterns for the species. Ag-staining for nucleolus organizer regions (AgNOR) was identified on the telomeric end of the long arm in 7 autosomal chromosomes. In this study we found more regions in chromosomes with staining than presented in the literature for the Bos indicus group (BIN). These NOR regions were repeated on the same chromosomes for the 4 animals studied.


2009 ◽  
Vol 45 (2) ◽  
pp. 77-86 ◽  
Author(s):  
Takakibi Mishima ◽  
Hiromu Katamoto ◽  
Yoichiro Horii ◽  
Victor A.M. Kakengi ◽  
Akira Ito

2015 ◽  
Vol 27 (1) ◽  
pp. 193
Author(s):  
P. K. Fontes ◽  
A. C. S. Castilho ◽  
R. F. P. Pinto ◽  
L. A. Trinca ◽  
R. F. Carvalho ◽  
...  

The oviduct plays a key role promoting a favourable microenvironment to gametes transport, fertilization and early embryo development. Numerous differences in reproductive physiology are known among animals of Zebu and European breeds. Reports indicate that female Zebu cattle have a higher number of follicles per wave than female European cattle and individual distinctions in the number of follicles recruited are present in both breeds, namely animals with high follicular count (HFC) and low follicular count (LFC). Furthermore, the follicular count is related to animal fertility and is greatly influenced by the activity of FSH, oestradiol, and androgens. However, little is known about the effects of follicular count differences between Zebu and European cattle, and between breeds in the oviduct molecular profile. Based in these information, we hypothesised that differences in bovine breed (Nelore and Aberdeen Angus), differences in the follicular count (FC), and differences in the antimere related to ovulation (ipsilateral and contralateral) alter the molecular profile of genes involved in oviducal functions during the initial period after ovulation. To do so, oviducts from Nelore heifers (HFC, n = 4; LFC, n = 4) and oviducts from Aberdeen Angus heifers (HFC, n = 4; LFC, n = 4) were isolated and oviducal segments were divided (infundibulum, ampulla, isthmus) from ipsilateral and contralateral antimere. Total RNA was extracted using Illustra TriplePrep Kit (GE Healthcare, Waukesha, WI, USA) and then reverse transcription was performed using a high-capacity cDNA kit (Life Technologies, Carlsbad, CA, USA), according to the manufacturer's protocols. Relative RT-qPCR analysis was performed with TaqMan® Low Density Array (TLDA, Life Technologies). The mRNA abundance of the target was tested by ANOVA analysis, using PROC GLM procedure of SAS (SAS, 9.2, SAS Institute Inc., Cary, NC, USA). Individual differences were analysed through pair-wise comparisons (SAS). All the comparisons were performed in each segment (ampulla, infundibulum, and isthmus); no comparisons were performed between segments. The differences were considered significant when P < 0.05. In the ampulla, the mRNA abundance of COX2, OVGP1, GPR78, FUCA1, and ANXA4 showed higher levels in ipsilateral antimere compared to contralateral. Similarly, in the infundibulum the mRNA abundance of GRP78, PGTER4, FUCA2, and FUCA1 was higher in ipsilateral antimere. No difference was found in the isthmus. In conclusion, the breed and the follicular count have no effect on the molecular profile of bovine oviduct, suggesting the site of ovulation has the main effect in gene expression related to gametes transport, fertilization, and early embryo development.Research supported by FAPESP 2012/09498-9 and 2012/50514-8.


2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Emanuel M. Doroteu ◽  
Joao H. M. Viana ◽  
Jair A. Ferreira Junior ◽  
Juliana T. A. Macedo ◽  
Rodrigo A. Oliveira ◽  
...  

AbstractThe aim of this study was to compare testicle morpho-functional characteristics in bulls undergoing a single or two immunizations against GnRH. Nelore (Bos taurus indicus) bulls were randomly allocated into three experimental groups: G1 (n=12), a single 400 μg dose of anti-GnRH vaccine on day 0; G2 (n=11), a first 400 μg dose of anti-GnRH vaccine on day 0 followed by a second (boost) dose 30 days later; and control group (CG, n=12), 1 mL saline 0.9% at day 0. Every 30 days, from day 0 until slaughter at day 90, the bulls were weighed and underwent testicular biometry, semen collection and analysis, and blood sample collection for testosterone measurement. Immediately after slaughter, the testicles were removed and transport at 15°C to the laboratory for histopathological analysis. There was a decrease in testicular height (P=0.0476), width (P=0.0021), and in scrotal circumference (P=0.0001), after either a single (G1) or two (G2) immunizations against GnRH. Both G1 and G2 had lower testosterone concentrations than CG from day 60 on (P<0.01), but in G2, it was also lower than in G1 at day 90 (P=0.0006). All sperm parameters were affected by active immunization against GnRH (P<0.05), and in G2, averages were lesser (P<0.05) than in G1 from day 60 on. No signs of seminiferous tubule degeneration were found in any sample from the CG, contrasting with 75.0% and 100.0% of the samples from G1 and G2, respectively. In summary, immunocastration affected testicle morpho-functional characteristics in bulls in a time- and dose-dependent way.


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