Cytogenetic and molecular analysis of the Pantaneiro cattle breed

The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin, indicating that the participation of zebuines in the formation of the breed occurred entirely through the paternal line.

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Whole genome characterization of autochthonous Bos taurus brachyceros and introduced Bos indicus indicus cattle breeds in Cameroon regarding their adaptive phenotypic traits and pathogen resistance

10.21203/rs.2.20033/v1 ◽

2020 ◽

Author(s):

Archile Eric paguem ◽

Babette Abanda ◽

Mbunkah Daniel Achukwi ◽

Praveen Baskaran ◽

Stefan Czemmel ◽

...

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Phenotypic Traits ◽

Zebu Cattle ◽

Whole Genome ◽

Genetic Traits ◽

Cattle Breeds ◽

Taurine Breed ◽

Taurine Cattle

Abstract BackgroundWest African indigenous taurine cattle display unique adaptive traits shaped by husbandry management, regional climate and exposure to endemic pathogens. They are less productive with respect to milk and meat production which has been associated with a number of factors, amongst others small size, traditional beliefs and husbandry practices. This resulted in the severe dwindling of their populations size rendering them vulnerable to extinction. The Namchi (Doayo) taurine cattle breed has documented resistance traits against trypanosome infection and exposure to tick infestation. Nonetheless, the historically later introduced Zebu cattle are the main cattle breeds in Africa today, even though they suffer more from locally prevailing pathogens. By using a reference-based whole genome sequencing approach, we sequenced for the first time the genomes of five cattle breeds from Cameroon: the Namchi (Doayo), an endangered trypanotolerant taurine breed, the Kapsiki, an indigenous trypanosusceptible taurine breed, and three Zebu (Bos indicus indicus) breeds: Ngaoundere Gudali, White Fulani and Red Fulani.ResultsApproximately 167 Giga bases of raw sequencing data were generated and mapped to the cattle reference genome UMD3.1. The coverage was 22 to 30-fold. The single nucleotide polymorphisms (SNPs) were compared with reference genomes of European Bos taurus Holstein and of Asian Bos indicus Brahman and the African trypanotolerant N’Dama breeds.Of a total of 50 million SNPs identified, 3.43 million were breed-specific ranging from 0.37 to 0.47 million SNPs in the domestic Cameroonian breeds and approximately 0.58 million constituted of small insertions and deletions. We identified breed specific-non-synonymous variants as genetic traits that could explain certain cattle-breed specific phenotypes such as increased tolerance against trypanosome parasites in the Namchi (Doayo) breed, heat tolerance in the Kapsiki breed, and growth, metabolism and meat quality in the Gudali breeds. Phylogenetic comparison grouped Namchi (Doayo) to the African Zebu clade indicating a hybrid status of the selected animal with a Zebu breed, albeit it showed the Namchi breed’s phenotype.ConclusionsThe findings provide the first comprehensive set of full genome variant data of the most important Cameroonian cattle breeds. The genomic data shall constitute a foundation for breed amelioration whilst exploiting the heritable traits and support conservation efforts for the endangered local cattle breeds.

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Phenotypic characterization of the Saudi Arabian Hassawi cattle breed

Animal Genetic Resources Information ◽

10.1017/s1014233900000900 ◽

1997 ◽

Vol 21 ◽

pp. 35-42 ◽

Cited By ~ 4

Author(s):

T. A. Mohammed

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Farming System ◽

Phenotypic Characterization ◽

Zebu Cattle ◽

Body Conformation ◽

High Feed ◽

Farming Families

SummaryHassawi cattle breed is a mix of Bos indicus and Bos taurus. The cattle are raised in the Eastern province of the country by farming families in mixed farming system. The breed numbers are declining very fast, from 10 449 head in 1986 to an estimated maximum of 4 500 head at present.The decrease is mainly due to replacement by exotic breeds, the indiscriminate crossing with these exotics, particularly in view of the scarcity of the Hassawi bulls for mating. Animals are small in size, mature body weight 210-270 kg for bulls and 150-200 kg for cows, quite uniform in colour (light red) and body conformation have conspicuously reduced dewlap and umbilical folds and relatively large hump. Animals are heat tolerant, sustain high feed intake under ambient temperature, resistant to many diseases prevailing in the region and cows have good mothering ability. Productivity of the breed in terms of meat and milk is low when compared to that of exotics in high input production environments, but reproduction performance excels that of temperate breeds and zebu cattle.Efforts should be made to stop the decline in the breed numbers and to conserve the breed as an asset for production under harsh environment.

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Identificación de ADN mitocondrial Bos taurus en poblaciones de ganado Cebú Brahman colombiano

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol7_num2_art:66 ◽

2007 ◽

Vol 7 (2) ◽

pp. 21

Author(s):

Gloria Patricia Barrera ◽

Rodrigo Alfredo Martínez ◽

Manuel Fernando Ariza

Keyword(s):

Mitochondrial Dna ◽

Genetic Divergence ◽

Bos Taurus ◽

Consensus Sequence ◽

Bos Indicus ◽

Zebu Cattle ◽

American Continent ◽

Brahman Cattle ◽

D Loop ◽

Dna Fragment

El continente americano fue colonizado en el siglo XVI por los europeos quienes introdujeron por primera vez el ganado bovino de origen Bos taurus. La introducción de ganado Bos indicus ocurrió muchos años después, con las primeras importaciones desde la India, las cuales incluyeron principalmente machos. Con el fin de estudiar la participación de hembras Bos taurus en el origen del ganado Cebú colombiano, se secuenció un fragmento del ADN mitocondrial de 374 pb (D-Loop) en seis animales de la raza Cebú Brahman colombiano y 20 individuos representativos de las cinco razas criollas colombianas: seis de Blanco Orejinegro (BON), cinco de Costeño con Cuernos (CCC), tres de Romosinuano (ROMO), cuatro de Casanareño (CAS) y dos de San Martinero (SM). Adicionalmente, para el mismo fragmento se secuenciaron dos individuos de la raza española Pirenaica, como referente Bos taurus. La comparación de las secuencias reveló que los animales de la raza Cebú Brahman colombiano analizados presentaron ADN mitocondrial de origen taurino con mayor cercanía respecto de las razas criollas de origen Bos taurus europeo que con relación a las secuencias consenso Bos indicus, frente a las que se hallaron mayores divergencias. Adicionalmente, las divergencias de las razas criollas colombianas con respecto al consenso Bos taurus europeo variaron entre 0,005 y 0,014, resultado que sugiere la participación de matrilineajes Bos taurus en el origen del Cebú Brahman colombiano. Identification of mitochondrial DNA of Bos taurus origin in Colombian Zebu Brahman cattle The American continent was colonised in the XVI century by the Europeans who introduced the Bos taurus cattle. The introduction of Bos indicus cattle was done a few years later with cattle from India, mainly males. In order to study the participation of Bos Taurus females in the origin of the Colombian Zebu cattle, a 374 bp mitochondrial DNA fragment was sequenced (D-Loop) in six animals belonging to Colombian Zebu Brahman breed and 20 individuals representative of he five Colombian native breeds: 6 of Blanco Orejinegro (BON), five of Costeño Con Cuernos (CCC), three of Romosinuano (ROMO), four of Casanareño (CAS) and two of San Martinero (SM). As a reference to Bos taurus, two individuals of the Spanish Pirenaica breed were also sequenced for the same fragment. Comparison between sequences revealed that the Zebu Brahman cattle has mitochondrial DNA of Bos Taurus origin and closer to the native breeds of Spanish origin. Although described as Bos indicus, it showed the lowest genetic divergence when compared with the consensus sequence of European Bos taurus. The genetic divergences of the Colombian native breeds compared with the European Bos Taurus ranged between 0.005 and 0.014. This suggests the participation of Bos taurus matrilineages in the origin of the Colombian Zebu Brahman cattle.

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Morphological dimorphism in the Y chromosome of "pé-duro" cattle in the Brazilian State of Piauí

Genetics and Molecular Biology ◽

10.1590/s1415-47571999000300014 ◽

1999 ◽

Vol 22 (3) ◽

pp. 369-373 ◽

Cited By ~ 6

Author(s):

Carmen M.C. Britto ◽

Maria Luiza S. Mello

Keyword(s):

Y Chromosome ◽

Coat Color ◽

Bos Taurus ◽

Chromosome Morphology ◽

Northeastern Brazil ◽

Zebu Cattle ◽

European Origin ◽

Brazilian State ◽

Y Chromosomes ◽

Bos Taurus Indicus

"Pé-duro" (hard foot) is a rare breed of beef cattle of European (Bos taurus taurus) origin, originated in northern and northeastern Brazil. Y chromosome morphology, outer genital elements and other phenotypic characteristics were examined in 75 "pé-duro" bulls from the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) herd in the Brazilian State of Piauí. The purpose was to investigate possible racial contamination with Zebu animals (Bos taurus indicus) in a cattle that has been considered closest to its European origin (B. t. taurus). The presence of both submetacentric and acrocentric Y chromosomes, typical of B. t. taurus and B. t. indicus, respectively, and the larger preputial sheath in bulls with an acrocentric Y chromosome indicated racial contamination of the "pé-duro" herd with Zebu cattle. Phenotypic parameters involving horn, dewlap, ear, chamfer, and coat color characteristics, indicative of apparent racial contamination, were not associated with acrocentric Y chromosome.

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Y chromosome variants in cattle Bos taurus and Bos indicus

Genetics Selection Evolution ◽

10.1186/1297-9686-14-1-1 ◽

1982 ◽

Vol 14 (1) ◽

pp. 1 ◽

Cited By ~ 3

Author(s):

CRE Halnan ◽

Janine I Watson

Keyword(s):

Y Chromosome ◽

Bos Taurus ◽

Bos Indicus

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Kazakhstani native cattle reveal highly divergent mtDNA from Bos taurus and Bos indicus lineages with an absence of Bos indicus Y chromosome

Animal Science Journal ◽

10.1111/asj.13128 ◽

2018 ◽

Vol 90 (1) ◽

pp. 29-34

Author(s):

Hayate Yamanaka ◽

Kako Murata ◽

Risa Tabata ◽

Fuki Kawaguchi ◽

Shinji Sasazaki ◽

...

Keyword(s):

Y Chromosome ◽

Bos Taurus ◽

Bos Indicus ◽

Native Cattle

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T-Cell and Antibody Responses to Mycobacterial Antigens in Tuberculin Skin-Test-PositiveBos indicusandBos taurusCattle in Ethiopia

Veterinary Medicine International ◽

10.1155/2012/457872 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Gobena Ameni ◽

Paul Cockle ◽

Konstantin Lyashchenko ◽

Martin Vordermeier

Keyword(s):

T Cell ◽

Skin Test ◽

Bos Taurus ◽

Bos Indicus ◽

Antigen Recognition ◽

Antibody Responses ◽

Zebu Cattle ◽

Mycobacterial Antigen ◽

Multiple Antigen ◽

Mycobacterial Antigens

Higher IFN-γresponses to mycobacterial antigens were observed inBos taurus(Holsteins) than inBos indicus(Zebu) cattle which could due to differences in antigen recognition profiles between the two breeds. The present study was conducted to evaluate mycobacterial antigen recognition profiles of the two breeds. Twenty-three mycobacterial antigens were tested on 46 skin test positive (24 Zebu and 22 Holstein) using enzyme-linked immunospot assay (ELISPOT) and multiple antigen print immunoassay (MAPIA). Herds from which the study cattle obtained were tested for Fasciola antibody. The T cells from both breeds recognized most of the mycobacterial antigens at lower and comparable frequencies. However, antigens such as CFP-10, ESAT-6, Rv0287, Rv0288, MPB87, Acr-2, Rv3616c, and Rv3879c were recognized at higher frequencies in zebu while higher frequencies of T cell responses were observed to Hsp65 in both breeds. Furthermore, comparable antibody responses were observed in both breeds; MPB83 being the sero-dominant antigen in both breeds. The prevalence of Fasciola antibody was 81% and similar in both breeds. This piece of work could not lead to a definitive conclusion if there are differences in mycobacterial recognition profiles between the two breeds warranting for further similar studies using sound sample size from the two breeds.

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Productivity of European cattle [Bos taurus], Zebu cattle [Bos indicus] and their crosses kept at the Llanos of Venezuela. I. Reproductive efficiency.

Theriogenology ◽

10.1016/0093-691x(75)90165-x ◽

1975 ◽

Vol 3 (3) ◽

pp. 123

Author(s):

W. Jöchle

Keyword(s):

Bos Taurus ◽

Bos Indicus ◽

Reproductive Efficiency ◽

Zebu Cattle ◽

European Cattle

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Genetic structure of the Southern meat cattle breed based on microsatellite markers

Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies ◽

10.32718/nvlvet-a9104 ◽

2019 ◽

Vol 21 (91) ◽

pp. 21-28

Author(s):

A. S. Kramarenko

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Microsatellite Markers ◽

Bos Taurus ◽

Bos Indicus ◽

Cattle Breed ◽

Animal Husbandry ◽

State Enterprise ◽

Tissue Samples ◽

Cattle Breeds

The Southern Meat cattle is a composite breed developed by crossing Cuban zebu (Bos indicus) with different cattle breeds (Bos taurus) – local the Red Steppe, Hereford, Charolais, Santa Gertrudis, Dairy Shorthorn. Genetic structure of the Southern meat cattle breed from the State Enterprise Experimental Farm “Askaniyske” NAAS Ukraine (Kherson region) were investigated based on the microsatellite DNA loci. Analysis included 192 animals. A panel of 12 bovine-speciﬁc microsatellite markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA122, INRA23, TGLA126, BM1818, ETH3, ETH225 and BM1824), recommended of the ISAG for cattle genetic diversity studies, was selected for genetic characterization and revealing the extent of genetic diversity in the Southern Meat cattle breed. Genomic DNA was extracted from tissue samples using Nexttec column (Nexttec Biotechnology GmbH, Germany) following the manufacturer's instructions. All laboratory tests were conducted in the laboratory of Molecular Genetics, Animal Center of Biotechnology and Molecular Diagnostics, All-Russian Research Institute for Animal Husbandry named after academy member L.K. Ernst. We report the distribution and the frequency of a taurine and an indicine specific alleles in the Southern Meat cattle breed using literature data about the Zebu and different cattle breeds genetic structure based on microsatellite loci from our list. It can be assumed that the TGLA22777, BM2113141-143, ETH10209-211, TGLA122149, INRA23194-198, TGLA126123, ETH225156-158-160 alleles among the Southern Meat cattle breed examined individuals were inherited from a B. indicus ancestor. On the other hand, the TGLA53156, ETH10217-219, TGLA122143, INRA23202, TGLA126115, ETH225148-150, BM1824188-190 alleles in the Southern Meat cattle gene pool may be inherited from a B. taurus ancestor (i.e., taurine breeds diagnostic alleles).

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