scholarly journals Banded karyotype of Nelore cattle (Bos taurus indicus Linnaeus, 1758)

2019 ◽  
Vol 13 (3) ◽  
pp. 265-275
Author(s):  
Andréia Pires Amancio ◽  
Sabrina Sara Moreira Duarte ◽  
Rafael Carneiro Silvafael ◽  
Alex Silva da Cruz ◽  
Danilo Conrado Silva ◽  
...  
Keyword(s):  
Chromosome Banding ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Culture Method ◽  
Nucleolus Organizer ◽  
Zebu Cattle ◽  
Metaphase Chromosomes ◽  
Nucleolus Organizer Regions ◽  
Bos Taurus Indicus ◽  
Nelore Cattle

Chromosome banding techniques were applied and standardized to obtain karyotype characteristics for the first time in Brazil of Nelore cattle – Bos taurus indicus Linnaeus, 1758 – (bovine subspecies most prominent in Brazilian livestock). Blood samples were collected from the animals of the School of Agrarian and Biological Sciences of the Pontifical Catholic University of Goiás, two males and two females of pure breed. These samples were submitted to the cell culture method to study metaphase chromosomes. Chromosome banding techniques (C, G and NOR) revealed the karyotype architecture of Nelore cattle common with that of other breeds of zebu cattle formerly karyotyped. The diploid chromosome number was invariably normal, 2n = 60. C-banding revealed C-positive heterochromatin in centromeric regions almost in all chromosomes. G-banding presented the expected band pattern in the respective chromosome pairs in correspondence with the established chromosomal patterns for the species. Ag-staining for nucleolus organizer regions (AgNOR) was identified on the telomeric end of the long arm in 7 autosomal chromosomes. In this study we found more regions in chromosomes with staining than presented in the literature for the Bos indicus group (BIN). These NOR regions were repeated on the same chromosomes for the 4 animals studied.

scholarly journals Cytogenetic and molecular analysis of the Pantaneiro cattle breed

2006 ◽  
Vol 41 (11) ◽  
pp. 1609-1615 ◽  
Author(s):  
Érica Cunha Issa ◽  
Wilham Jorge ◽  
José Robson Bezerra Sereno
Keyword(s):  
Mitochondrial Dna ◽  
Y Chromosome ◽  
Molecular Analysis ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cattle Breed ◽  
Zebu Cattle ◽  
European Origin ◽  
Paternal Line

The objective of this work was to characterize Pantaneiro cattle genetically through its paternal ancestry by the morphology of the Y chromosome, whether submetacentric or acrocentric, as well as to identify the maternal ancestry through mitochondrial DNA. The karyotype and mitochondrial DNA of 12 bulls of Pantaneiro breed were analyzed. The Y chromosome was analyzed in lymphocyte metaphases and the mitochondrial DNA by diagnosing its haplotype (Bos taurus and Bos indicus). Among Pantaneiro animals analyzed three had a taurine (submetacentric) Y and nine had a zebuine (acrocentric) Y chromosome, suggesting breed contamination by Zebu cattle, once Pantaneiro is considered to be of European origin. The mitochondrial DNA was exclusively of taurine origin, indicating that the participation of zebuines in the formation of the breed occurred entirely through the paternal line.

scholarly journals T-Cell and Antibody Responses to Mycobacterial Antigens in Tuberculin Skin-Test-PositiveBos indicusandBos taurusCattle in Ethiopia

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Gobena Ameni ◽  
Paul Cockle ◽  
Konstantin Lyashchenko ◽  
Martin Vordermeier
Keyword(s):  
T Cell ◽  
Skin Test ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Antigen Recognition ◽  
Antibody Responses ◽  
Zebu Cattle ◽  
Mycobacterial Antigen ◽  
Multiple Antigen ◽  
Mycobacterial Antigens

Higher IFN-γresponses to mycobacterial antigens were observed inBos taurus(Holsteins) than inBos indicus(Zebu) cattle which could due to differences in antigen recognition profiles between the two breeds. The present study was conducted to evaluate mycobacterial antigen recognition profiles of the two breeds. Twenty-three mycobacterial antigens were tested on 46 skin test positive (24 Zebu and 22 Holstein) using enzyme-linked immunospot assay (ELISPOT) and multiple antigen print immunoassay (MAPIA). Herds from which the study cattle obtained were tested for Fasciola antibody. The T cells from both breeds recognized most of the mycobacterial antigens at lower and comparable frequencies. However, antigens such as CFP-10, ESAT-6, Rv0287, Rv0288, MPB87, Acr-2, Rv3616c, and Rv3879c were recognized at higher frequencies in zebu while higher frequencies of T cell responses were observed to Hsp65 in both breeds. Furthermore, comparable antibody responses were observed in both breeds; MPB83 being the sero-dominant antigen in both breeds. The prevalence of Fasciola antibody was 81% and similar in both breeds. This piece of work could not lead to a definitive conclusion if there are differences in mycobacterial recognition profiles between the two breeds warranting for further similar studies using sound sample size from the two breeds.

scholarly journals Frecuencias alélicas para variantes SNP en el gen Nramp1 en bovinos infectados con Brucella abortus o clasificados por resistencia al patógeno

2009 ◽  
Vol 10 (1) ◽  
pp. 43
Author(s):  
Fernando Cerquera M. ◽  
Rodrigo Martínez S. ◽  
Rubén Toro O. ◽  
Jaime Tobón C. ◽  
Jaime Gallego G. ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Brucella Abortus ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Natural Resistance ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Allelic Frequencies ◽  
Bos Taurus Indicus

<p>La resistencia natural a la brucelosis en bovinos ha sido asociada a factores genéticos, principalmente a algunos polimorfismos de nucleótido simple ubicados dentro del gen Nramp1. La presente investigación evalúa el efecto de variantes tipo polimorfismos de nucleótido simple presentes en regiones codificantes y en la región 3’UTR del gen Nramp1, en la clasificación de los animales como resistentes o susceptibles; además se determinan los genotipos predominantes en animales naturalmente infectados y comprobados como positivos por la presencia de anticuerpos anti <em>Brucella abortus</em>. Se establecieron las frecuencias genotípicas y alélicas para cinco polimorfismos de nucleótido simple identificados dentro del gen Nramp1 en animales de las razas blanco orejinegro (<em>Bos taurus taurus</em>) y cebú (<em>Bos taurus indicus</em>) y en muestras serológicamente positivas provenientes de animales cruzados (<em>Bos taurus </em>x <em>Bos indicus</em>). La determinación de genotipos se realizó mediante la metodología polimorfismo conformacional de cadena sencilla. Se realizó un ensayo de desafío infeccioso in vitro, para estimar la capacidad de los macrófagos bovinos para controlar la sobrevivencia bacterial, lo que permitió definir los individuos como resistentes o susceptibles. Los resultados sugieren una asociación significativa del SNP4 (<em>p </em>= 0,0506) con la variación para el fenotipo de susceptibilidad, pues se encontró el genotipo homocigoto (BB) en alta frecuencia en animales catalogados como resistentes y el genotipo heterocigoto (AB) en alta frecuencia en animales catalogados como susceptibles y en animales con títulos de anticuerpos anti <em>Brucella abortus</em>.  </p><p> </p><p><strong>Allelic frequencies for SNP variants in the gene Nramp1 in bovine infected with </strong><strong><em>Brucella abortus </em></strong><strong>or classified by resistance to the pathogen</strong>  </p><p>The natural resistance to brucellosis in cattle has been associated to genetic factors mainly to some single nucleotide polymorphism (SNP), located within Nramp1 gen. The current research has studied the effect of nucleotide variants to be found in coding regions and other one located in 3 non translated region of Nramp1 gene, on the animal classification as resistant or susceptible, moreover was identified the main genotypes to be found on the infected animals, confirmed as positives by antibody antibrucella titles. Was established the genotypic and allelic frequencies for five single nucleotide polymorphism in animals from blanco orejinegro (<em>Bos taurus taurus</em>) and zebu breeds (<em>Bos taurus indicus</em>) and serum samples belonging to positive crossbred animals (<em>Bos taurus x Bos indicus</em>). The genotype was defined by the methodology known as “single strand conformational polymorphism”. To estimate the macrophage capacity to control the bacterial survival, an in vitro assay was performed, which allowed define the phenotype as resistant or susceptible. The results suggest a significant association for SNP4 (p = 0.0506) with the phenotypic variation for resistant or susceptibility, because was found the genotype (BB) at higher frequency in susceptible animals and naturally infected animals, than those resistant animals. </p>

scholarly journals Whole genome characterization of autochthonous Bos taurus brachyceros and introduced Bos indicus indicus cattle breeds in Cameroon regarding their adaptive phenotypic traits and pathogen resistance

2020 ◽  
Author(s):  
Archile Eric paguem ◽  
Babette Abanda ◽  
Mbunkah Daniel Achukwi ◽  
Praveen Baskaran ◽  
Stefan Czemmel ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Cattle Breed ◽  
Phenotypic Traits ◽  
Zebu Cattle ◽  
Whole Genome ◽  
Genetic Traits ◽  
Cattle Breeds ◽  
Taurine Breed ◽  
Taurine Cattle

Abstract BackgroundWest African indigenous taurine cattle display unique adaptive traits shaped by husbandry management, regional climate and exposure to endemic pathogens. They are less productive with respect to milk and meat production which has been associated with a number of factors, amongst others small size, traditional beliefs and husbandry practices. This resulted in the severe dwindling of their populations size rendering them vulnerable to extinction. The Namchi (Doayo) taurine cattle breed has documented resistance traits against trypanosome infection and exposure to tick infestation. Nonetheless, the historically later introduced Zebu cattle are the main cattle breeds in Africa today, even though they suffer more from locally prevailing pathogens. By using a reference-based whole genome sequencing approach, we sequenced for the first time the genomes of five cattle breeds from Cameroon: the Namchi (Doayo), an endangered trypanotolerant taurine breed, the Kapsiki, an indigenous trypanosusceptible taurine breed, and three Zebu (Bos indicus indicus) breeds: Ngaoundere Gudali, White Fulani and Red Fulani.ResultsApproximately 167 Giga bases of raw sequencing data were generated and mapped to the cattle reference genome UMD3.1. The coverage was 22 to 30-fold. The single nucleotide polymorphisms (SNPs) were compared with reference genomes of European Bos taurus Holstein and of Asian Bos indicus Brahman and the African trypanotolerant N’Dama breeds.Of a total of 50 million SNPs identified, 3.43 million were breed-specific ranging from 0.37 to 0.47 million SNPs in the domestic Cameroonian breeds and approximately 0.58 million constituted of small insertions and deletions. We identified breed specific-non-synonymous variants as genetic traits that could explain certain cattle-breed specific phenotypes such as increased tolerance against trypanosome parasites in the Namchi (Doayo) breed, heat tolerance in the Kapsiki breed, and growth, metabolism and meat quality in the Gudali breeds. Phylogenetic comparison grouped Namchi (Doayo) to the African Zebu clade indicating a hybrid status of the selected animal with a Zebu breed, albeit it showed the Namchi breed’s phenotype.ConclusionsThe findings provide the first comprehensive set of full genome variant data of the most important Cameroonian cattle breeds. The genomic data shall constitute a foundation for breed amelioration whilst exploiting the heritable traits and support conservation efforts for the endangered local cattle breeds.

scholarly journals Comparative analysis based on replication banding reveals the mechanism responsible for the difference in the karyotype constitution of treefrogs Ololygon and Scinax (Arboranae, Hylidae, Scinaxinae)

2017 ◽  
Vol 11 (2) ◽  
pp. 267-283 ◽  
Author(s):  
Simone Lilian Gruber ◽  
Gabriela Isabela Gomes de Oliveira ◽  
Ana Paula Zampieri Silva ◽  
Hideki Narimatsu ◽  
Célio Fernando Baptista Haddad ◽  
...  
Keyword(s):  
Chromosome Banding ◽  
Diploid Number ◽  
Nucleolus Organizer ◽  
Replication Banding ◽  
Nucleolus Organizer Regions ◽  
The Family ◽  
The Difference ◽  
Size And Morphology ◽  
Phylogenetic Revision ◽  
Dna Incorporation

According to the recent taxonomic and phylogenetic revision of the family Hylidae, species of the former Scinaxcatharinae (Boulenger, 1888) clade were included in the resurrected genus Ololygon Fitzinger, 1843, while species of the Scinaxruber (Laurenti, 1768) clade were mostly included in the genus Scinax Wagler, 1830, and two were allocated to the newly created genus JulianusDuellman et al., 2016. Although all the species of the former Scinax genus shared a diploid number of 2n = 24 and the same fundamental number of chromosome arms of FN = 48, two karyotypic constitutions were unequivocally recognized, related mainly to the distinct size and morphology of the first two chromosome pairs. Some possible mechanisms for these differences had been suggested, but without any experimental evidence. In this paper, a comparison was carried out based on replication chromosome banding, obtained after DNA incorporation of 5-bromodeoxiuridine in chromosomes of Ololygon and Scinax. The obtained results revealed that the loss of repetitive segments in chromosome pairs 1 and 2 was the mechanism responsible for karyotype difference. The distinct localization of the nucleolus organizer regions in the species of both genera also differentiates the two karyotypic constitutions.

223 PREANTRAL FOLLICLES POPULATION IN BOS TAURUS INDICUS AND BOS TAURUS TAURUS FEMALES

2010 ◽  
Vol 22 (1) ◽  
pp. 269
Author(s):  
K. C. F. Silva ◽  
G. M. G. Santos ◽  
L.S. Siloto ◽  
M. F. Hertel ◽  
E. R. Andrade ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Bos Taurus ◽  
Science And Technology ◽  
Bos Indicus ◽  
Histological Evaluation ◽  
National Council ◽  
Crown Rump Length ◽  
Preantral Follicles ◽  
Oocyte Yield ◽  
Bos Taurus Indicus

Bos taurus indicus females provide a greater in vivo oocyte recovery (2-4 times more) in comparision with Bos taurus taurus. This aspect has strongly contributed for the success of the embryo industry in Brazil. Little information is available to explain this difference. Our objective was to test the hypothesis that the difference in oocyte yield is a result of higher numbers of preantral follicles in the ovaries of B. indicus females. Ovaries (n = 44) of fetuses aging from 180 to 240 days and of heifers from 20 and 24 months were collected from Bos taurus indicus (Nelore) and Bos taurus taurus (Aberdeen Angus) females at a slaughterhouse. Fetal age was estimated from the crown-rump length (Evans HE and Sack WO 1973 Anat. Hist. Embryol. 2, 11-45). Ovaries from Nelore fetuses (n = 10) and heifers (n = 12) and Aberdeen Angus fetuses (n = 10) and heifers (n = 12) were cut longitudinally into two halves, fixed in Bouin’s solution, and processed for histological evaluation. The ovarian halves were dehydrated in alcohol, cleared with xylene, embbebed in paraffin, and serially sectioned at 7 μm. Every 120th section was mounted and stained with periodic acid Schiff and hematoxylin. The number of preantral follicles was estimated through the counting of follicles in each section using the nucleus of the oocyte as a marker and a correction factor (Gougeon A and Chainy GBN 1987 J. Reprod. Fertil. 81, 433-442). Only 1 ovary per female was analyzed. Preantral follicles were classified according to the developmental stage as primordial (1 layer of flattened granulosa cells surrounding the oocyte), primary (1 layer of cuboidal granulosa cells), or secondary (2 or more layers of cuboidal granulosa cells), and as normal or degenerated according to their morphological appearance. The number of preantral follicles was not normally distributed and was compared using the Mann-Whitney test. There was no difference (P > 0.05) between the average number of preantral follicles in the ovaries of Bos indicus and Bos taurus females. The average number of preantral follicles per female was 143 929 ± 253 (mean ± SD) and 285 155 ± 570 for Bos taurus and Bos indicus fetuses, respectively, and 76 851 ± 280 and 109 673 ± 293 for Bos taurus and Bos indicus heifers (P > 0.05), respectively. A large variation in numbers of preantral follicles was observed among individuals within the same category and between breeds. Our results suggest that there might be differences in mechanisms controlling follicle development after the preantral stage accounting for the greater oocyte yield from Bos indicus females. This work was supported by grants from the Universidad Nacional del Litoral (CAI+D program), Universidad Nacional de Lomas de Zamora, the Argentine National Agency for the Promotion of Science and Technology (ANPCyT), and the Argentine National Council for Science and Technology (CONICET).

108 Synchronization and artificial insemination of South African communal cattle

2021 ◽  
Vol 33 (2) ◽  
pp. 161
Author(s):  
T. L. Magopa ◽  
M. L. Mphaphathi ◽  
T. Mulaudzi ◽  
F. V. Ramukhithi ◽  
M. M. Tshabalala ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Assisted Reproductive Technologies ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Reproductive Technologies ◽  
Computer Assisted ◽  
Bos Taurus Indicus ◽  
Oestradiol Benzoate

The present study aimed to evaluate an oestrous synchronization protocol and oestrus response before AI in cows from an organised communal production system. A total of 74 cows Bos indicus (Brahman) and Bos taurus/indicus hybrid (Nguni) type from different communal farmers were screened based on: age (3 to 8 years), body condition score of ≥3 (1–5 scale), not pregnant (excluding heifers), 90 days postpartum, number of parities, good mothering ability, and negative for contagious abortion. Selected cows were subjected to a 9-day OvSynch+CIDR protocol. In brief, the protocol included the insertion of controlled intravaginal drug release (CIDR®, Pfizer Laboratories) devices in the reproductive tract (vagina) containing 1.9g of progesterone in each cow on Day 0 with first oestradiol benzoate (Oestradiol benzoate®, VTech) 2-mL intramuscular (IM) injection. Pregnant mare serum gonadotrophin (PMSG; Chronogest®, Intervet International B.V.) 2.5-mL (IM) injection on Day 5. Removal of CIDR and (IM) injection of prostaglandin F2α (PGF2α; Estrumate®, Intervet South Africa (Pty.) Ltd.) 2mL on Day 8. Following CIDR removal, a heat mount detector (Kamar®) was applied on the individual cow’s tail head for oestrus observation (signs of heat) with second oestradiol benzoate 1-mL (IM) injection on Day 9. AI was performed 36h following withdrawal of the CIDR. Frozen/thawed semen from Bonsmara bulls (n=2) of known and proven fertility was used for AI. The GameteTek Cryo-Mobile laboratory was used during thawing of semen straws. and sperm motility and morphological traits were immediately evaluated by computer-assisted sperm analysis (Sperm Class Analyzer®) before each individual cow was inseminated. Pregnancy diagnosis was performed 90 days after timed AI by ultrasound and transrectal palpation. Data were analysed using the logistic regression procedure of SAS (SAS Institute Inc.), with synchronisation response and conception being treated as binary response variables. All cows were synchronized successfully and an oestrus response rate of 100% was recorded. Pregnancy rates were similar (37.8% vs. 38.9%) for both Bos indicus and Bos taurus/indicus hybrid cattle. Bulls total sperm motility of ≥75% were recorded, following thawing of semen straws. Thus, there was no effect of bull on pregnancy. In conclusion, acceptable oestrus synchronization response was achieved in this communal setup. Superior genetic materials can be successfully introduced through assisted reproductive technologies in organised communal production systems.

Phenotypic characterization of the Saudi Arabian Hassawi cattle breed

1997 ◽  
Vol 21 ◽  
pp. 35-42 ◽  
Author(s):  
T. A. Mohammed
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Cattle Breed ◽  
Farming System ◽  
Phenotypic Characterization ◽  
Zebu Cattle ◽  
Body Conformation ◽  
High Feed ◽  
Farming Families

SummaryHassawi cattle breed is a mix of Bos indicus and Bos taurus. The cattle are raised in the Eastern province of the country by farming families in mixed farming system. The breed numbers are declining very fast, from 10 449 head in 1986 to an estimated maximum of 4 500 head at present.The decrease is mainly due to replacement by exotic breeds, the indiscriminate crossing with these exotics, particularly in view of the scarcity of the Hassawi bulls for mating. Animals are small in size, mature body weight 210-270 kg for bulls and 150-200 kg for cows, quite uniform in colour (light red) and body conformation have conspicuously reduced dewlap and umbilical folds and relatively large hump. Animals are heat tolerant, sustain high feed intake under ambient temperature, resistant to many diseases prevailing in the region and cows have good mothering ability. Productivity of the breed in terms of meat and milk is low when compared to that of exotics in high input production environments, but reproduction performance excels that of temperate breeds and zebu cattle.Efforts should be made to stop the decline in the breed numbers and to conserve the breed as an asset for production under harsh environment.

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