scholarly journals Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep

2014 ◽  
Vol 43 (4) ◽  
pp. 183-187
Author(s):  
Ana Zuley Ruiz
Keyword(s):  
Receptor Subunit ◽  
Dna Encoding ◽  
Cloning And Sequencing ◽  
Aspartate Receptor ◽  
Male Sheep

scholarly journals Genomic Organization and Characterization of the white Locus of the Mediterranean Fruitfly, Ceratitis capitata

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1245-1255 ◽  
Author(s):  
L M Gomulski ◽  
R J Pitts ◽  
S Costa ◽  
G Saccone ◽  
C Torti ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Dna Encoding ◽  
Cloning And Sequencing ◽  
Deletion Event ◽  
Exon 2 ◽  
Independent Events ◽  
White Locus ◽  
Primary Amino ◽  
Organizational Patterns

Abstract An ∼14-kb region of genomic DNA encoding the wild-type white eye (w+) color gene from the medfly, Ceratitis capitata has been cloned and characterized at the molecular level. Comparison of the intron-exon organization of this locus among several dipteran insects reveals distinct organizational patterns that are consistent with the phylogenetic relationships of these flies and the dendrogram of the predicted primary amino acid sequence of the white loci. An examination of w+ expression during medfly development has been carried out, displaying overall similarity to corresponding studies for white gene homologues in Drosophila melanogaster and other insects. Interestingly, we have detected two phenotypically neutral allelic forms of the locus that have arisen as the result of an apparently novel insertion or deletion event located in the large first intron of the medfly white locus. Cloning and sequencing of two mutant white alleles, w1 and w2, from the we,wp and M245 strains, respectively, indicate that the mutant conditions in these strains are the result of independent events—a frameshift mutation in exon 6 for w1 and a deletion including a large part of exon 2 in the case of w2.

Expression of N-Methyl-D-Aspartate receptor subunit mRNAs in the human brain: Hippocampus and cortex

1998 ◽  
Vol 390 (1) ◽  
pp. 75-90 ◽  
Author(s):  
Clemens R. Scherzer ◽  
G. Bernhard Landwehrmeyer ◽  
Julie A. Kerner ◽  
Timothy J. Counihan ◽  
Christoph M. Kosinski ◽  
...  
Keyword(s):  
Human Brain ◽  
Receptor Subunit ◽  
Aspartate Receptor

The role of N-methyl-d -aspartate receptor subunit NR2B in spinal cord in cancer pain

2010 ◽  
Vol 14 (5) ◽  
pp. 496-502 ◽  
Author(s):  
XiaoPing Gu ◽  
Juan Zhang ◽  
ZhengLiang Ma ◽  
JunHua Wang ◽  
XiaoFang Zhou ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cancer Pain ◽  
Receptor Subunit ◽  
Aspartate Receptor

scholarly journals Intrathecal Injection of the ς1Receptor Antagonist BD1047 Blocks Both Mechanical Allodynia and Increases in Spinal NR1 Expression during the Induction Phase of Rodent Neuropathic Pain

2008 ◽  
Vol 109 (5) ◽  
pp. 879-889 ◽  
Author(s):  
Dae-Hyun Roh ◽  
Hyun-Woo Kim ◽  
Seo-Yeon Yoon ◽  
Hyoung-Sig Seo ◽  
Young-Bae Kwon ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Mechanical Allodynia ◽  
Maintenance Phase ◽  
Spinal Cord Dorsal Horn ◽  
Induction Phase ◽  
Receptor Subunit ◽  
Aspartate Receptor ◽  
Spinal Cord Dorsal

Background Selective blockade of spinal sigma(1) receptors (Sig-1R) suppresses nociceptive behaviors in the mouse formalin test. The current study was designed to verify whether intrathecal Sig-1R antagonists can also suppress chronic neuropathic pain. Methods Neuropathic pain was produced by chronic constriction injury (CCI) of the right sciatic nerve in rats. The Sig-1R antagonist BD1047 was administered intrathecally twice daily from postoperative days 0 to 5 (induction phase of neuropathic pain) or from days 15 to 20 (maintenance phase). Western blot and immunohistochemistry were performed to determine changes in Sig-1R expression and to examine the effect of BD1047 on N-methyl-D-aspartate receptor subunit 1 expression and phosphorylation in spinal cord dorsal horn from neuropathic rats. Results BD1047 administered on postoperative days 0-5 significantly attenuated CCI-induced mechanical allodynia, but not thermal hyperalgesia, and this suppression was blocked by intrathecal administration of the Sig-1R agonist PRE084. In contrast, BD1047 treatment during the maintenance phase of neuropathic pain had no effect on mechanical allodynia. Sig-1R expression significantly increased in the ipsilateral spinal cord dorsal horn from days 1 to 3 after CCI. Importantly, BD1047 (30 nmol) administered intrathecally during the induction, but not the maintenance phase, blocked the CCI-induced increase in N-methyl-D-aspartate receptor subunit 1 expression and phosphorylation. Conclusions These results demonstrate that spinal Sig-1Rs play a critical role in both the induction of mechanical allodynia and the activation of spinal N-methyl-d-aspartate receptors in CCI rats and suggest a potential therapeutic role for the use of Sig-1R antagonists in the clinical management of neuropathic pain.

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