scholarly journals Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation

2006 ◽  
Vol 49 (5) ◽  
pp. 699-704 ◽  
Author(s):  
Giovana Kirst Tychanowicz ◽  
Daniela F. de Souza ◽  
Cristina G. M. Souza ◽  
Marina Kimiko Kadowaki ◽  
Rosane Marina Peralta
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
High Resistance ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Rot Fungus ◽  
Corn Cob ◽  
Pleurotus Pulmonarius

Pleurotus pulmonarius (Fr) Quélet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.

scholarly journals Production of Manganese Peroxidase and Organic Acids and Mineralization of 14C-Labelled Lignin (14C-DHP) during Solid-State Fermentation of Wheat Straw with the White Rot Fungus Nematoloma frowardii

1999 ◽  
Vol 65 (5) ◽  
pp. 1864-1870 ◽  
Author(s):  
Martin Hofrichter ◽  
Tamara Vares ◽  
Mika Kalsi ◽  
Sari Galkin ◽  
Katrin Scheibner ◽  
...  
Keyword(s):  
Solid State ◽  
Organic Acids ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Manganese Peroxidase ◽  
Ligninolytic Enzyme ◽  
Water Soluble ◽  
White Rot ◽  
White Rot Fungus ◽  
Free System

ABSTRACT The basidiomycetous fungus Nematoloma frowardiiproduced manganese peroxidase (MnP) as the predominant ligninolytic enzyme during solid-state fermentation (SSF) of wheat straw. The purified enzyme had a molecular mass of 50 kDa and an isoelectric point of 3.2. In addition to MnP, low levels of laccase and lignin peroxidase were detected. Synthetic 14C-ring-labelled lignin (14C-DHP) was efficiently degraded during SSF. Approximately 75% of the initial radioactivity was released as14CO2, while only 6% was associated with the residual straw material, including the well-developed fungal biomass. On the basis of this finding we concluded that at least partial extracellular mineralization of lignin may have occurred. This conclusion was supported by the fact that we detected high levels of organic acids in the fermented straw (the maximum concentrations in the water phases of the straw cultures were 45 mM malate, 3.5 mM fumarate, and 10 mM oxalate), which rendered MnP effective and therefore made partial direct mineralization of lignin possible. Experiments performed in a cell-free system, which simulated the conditions in the straw cultures, revealed that MnP in fact converted part of the14C-DHP to 14CO2 (which accounted for up to 8% of the initial radioactivity added) and14C-labelled water-soluble products (which accounted for 43% of the initial radioactivity) in the presence of natural levels of organic acids (30 mM malate, 5 mM fumarate).

Lignocellulolytic activity of Pleurotus ostreatus under solid state fermentation using silage, stover, and cobs of maize

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3797-3807
Author(s):  
Magdah Ganash ◽  
Tarek M. Abdel Ghany ◽  
Mohamed A. Al Abboud ◽  
Mohamed M. Alawlaqi ◽  
Husam Qanash ◽  
...  
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Value Added ◽  
Extracellular Protein ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulosic Substrate

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.

Solid-state fermentation of lignocellulotic materials for the production of enzymes by the white-rot fungus Trametes hirsuta in a modular bioreactor

2011 ◽  
Vol 11 (4) ◽  
pp. 395-401 ◽  
Author(s):  
Ulrike Böhmer ◽  
Susanne Frömmel ◽  
Thomas Bley ◽  
Matthias Müller ◽  
Katrin Frankenfeld ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
White Rot ◽  
White Rot Fungus ◽  
Trametes Hirsuta

scholarly journals Decolorization of textile industry wastewater in solid state fermentation with Peach-Palm (Bactris gasipaes) residue

2018 ◽  
Vol 78 (4) ◽  
pp. 718-727 ◽  
Author(s):  
J. A. Chicatto ◽  
K. T. Rainert ◽  
M. J. Gonçalves ◽  
C. V. Helm ◽  
D. Altmajer-Vaz ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Industrial Effluent ◽  
Color Removal ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Bactris Gasipaes ◽  
Experimental Conditions ◽  
Peach Palm

Abstract In this work we have assessed the decolorization of textile effluents throughout their treatment in a solid-state fermentation (SSF) system. SSF assays were conducted with peach-palm (Bactris gasipaes) residue using the white rot fungus Ganoderma lucidum EF 31. The influence of the dye concentration and of the amounts of peach-palm residue and liquid phase on both the discoloration efficiency and enzyme production was studied. According to our results, independently of experimental conditions employed, laccase was the main ligninolytic enzyme produced by G. lucidum. The highest laccase activity was obtained at very low effluent concentrations, suggesting the existence of an inhibitory effect of higher concentrations on fungal metabolism. The highest percentage of color removal was reached when 10 grams of peach palm residue was moistened with 60 mL of the final effluent. In control tests carried out with the synthetic dye Remazol Brilliant Blue R (RBBR) decolorization efficiencies about 20% higher than that achieved with the industrial effluent were achieved. The adsorption of RBBR on peach-palm residue was also investigated. Equilibrium tests showed that the adsorption of this dye followed both Langmuir and Freundlich isotherms. Hence, our experimental results indicate that peach-palm residue is suitable substrate for both laccase production and color removal in industrial effluents.

Effect of Moisture Content and Inoculum Size on Cell Wall Composition and Ethanol Yield from Switchgrass after Solid-State Pleurotus ostreatus Treatment

2018 ◽  
Vol 61 (6) ◽  
pp. 1997-2006 ◽  
Author(s):  
Mengxing Li ◽  
Stephen M. Marek ◽  
Jiaqi Peng ◽  
Zhongdong Liu ◽  
Mark R. Wilkins
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Ethanol Production ◽  
Ethanol Yield ◽  
Perennial Grass ◽  
Ligninolytic Enzyme ◽  
Inoculum Size ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Pretreatment

Abstract. The white-rot fungus is capable of selectively degrading lignin over polymeric sugars. Solid-state cultivation and subsequent simultaneous saccharification and fermentation for ethanol production were performed. Effects of moisture content (MC) and fungus inoculum on biomass degradation, ligninolytic enzyme, and ethanol production were evaluated. First, fungal pretreatment was performed with varied MC and inoculum levels and sampled every 20 days. The highest xylose yield observed was 15.6% for samples with 75% MC and 5 mL inoculum at fungal pretreatment of 40 days. The highest lignin degradation of 52% and highest ethanol yield of 31% (based on the glucan present in the raw switchgrass) were achieved for 80-day fungal-treated samples with 75% MC and 5 mL inoculum. Keywords: KLywords. Bioenergy, Fungal pretreatment, Oyster mushroom, Perennial grass.

Sign in / Sign up

Export Citation Format

Share Document