scholarly journals Isolation and characterization by asymmetric PCR of the ENDO1 gene for glucan endo-1,3-β-D-glucosidase in Phytophthora cinnamomi associated with the ink disease of Castanea sativa Mill

2010 ◽  
Vol 53 (3) ◽  
pp. 513-518 ◽  
Author(s):  
Sofia Meirinho ◽  
Marisa Carvalho ◽  
Ángel Dominguez ◽  
Altino Choupina
Keyword(s):  
Genomic Dna ◽  
Gene Sequence ◽  
Phytophthora Cinnamomi ◽  
Castanea Sativa ◽  
European Regions ◽  
Asymmetric Pcr ◽  
Isolation And Characterization ◽  
Castanea Sativa Mill ◽  
Full Length Gene ◽  
Ink Disease

Ink disease is one of the most destructive diseases in Castanea sativa. The most common symptoms are root necrosies and a reduction in root growth, which invariably lead to the death of the trees. Phytophthora cinnamomi is an oomycete associated with this disease whose life cycle develops integrally in the soil. In the present work, was a fragment with 1231bp of the glucan endo-1,3-β-D-glucosidase gene obtained by amplification, using conserved primers and the full-length gene sequence by flanking this known sequence by asymmetric PCR. This fragment was obtained from genomic DNA of Phytophthora cinnamomi isolated in the European Regions of Castilla-Leon (Spain) and Trás-os-Montes (Portugal) and associated with the ink disease of Castanea sativa Mill.

scholarly journals Efficacy of biofungicides Supresivit and Polyversum against Phytophthora root pathogens on European chestnut (Castanea sativa Mill.)

2011 ◽  
Vol 31 (No. 3) ◽  
pp. 109-114
Author(s):  
G. Juhásová ◽  
S. Bernadovičová
Keyword(s):  
Trichoderma Harzianum ◽  
Mycelial Growth ◽  
Soil Fungi ◽  
Phytophthora Cinnamomi ◽  
Castanea Sativa ◽  
Pythium Oligandrum ◽  
European Chestnut ◽  
Significant Difference ◽  
Castanea Sativa Mill ◽  
Ink Disease

The effect of two commercially produced biopreparations on the originators of chestnut ink disease in Slovakia – soil fungi Phytophthora cambivora and Phytophthora cinnamomi was tested in laboratory conditions. Investigations of interrelations between Trichoderma harzianum (Pythium oligandrum) and isolates of Phytophthora sp. obtained from infected tissues of Castanea sativa Mill. proved more important inhibitive effects for Pythium oligandrum (biopreparation Polyversum) efficiency 25.3% (Ph. cambivora) and 26.2% (Ph. cinnamomi). Biopreparation Supresivit (Trichoderma harzianum) reached the efficiency 9.1% for Ph. cambivora isolates and 9.2% for Ph. cinnamomi isolates. Significant effect of the used bioformulas for inhibition of mycelial growth of both Phytophthora sp. and significant difference in the efficacy of two different bioformulas were confirmed statistically.  

SELECTION OF CASTANEA SATIVA MILL. FOR RESISTANCE TO PHYTOPHTHORA CINNAMOMI: TESTING OF SELECTED CLONES

2009 ◽  
pp. 395-404 ◽  
Author(s):  
B. Cuenca ◽  
M.R. Fernández ◽  
L. Ocaña ◽  
C. Salinero ◽  
C. Pintos ◽  
...  
Keyword(s):  
Phytophthora Cinnamomi ◽  
Castanea Sativa ◽  
Castanea Sativa Mill ◽  
Selection Of

SELECTION OF CASTANEA SATIVA MILL. GENOTYPES RESISTANT TO INK DISEASE IN GALICIA (SPAIN)

2005 ◽  
pp. 645-652 ◽  
Author(s):  
L. Rodriguez ◽  
B. Cuenca ◽  
C.A. López ◽  
F.J. Lario ◽  
L. Ocaña
Keyword(s):  
Castanea Sativa ◽  
Castanea Sativa Mill ◽  
Ink Disease ◽  
Selection Of

scholarly journals Loss of Aggressiveness of Phytophthora cinnamomi (Beta-Cinnamomin Silenced Strain) in the Infection of Castanea sativa

2012 ◽  
Vol 18 (S5) ◽  
pp. 17-18 ◽  
Author(s):  
I. Maia ◽  
M. Horta ◽  
A. Cravador ◽  
C. Medeira
Keyword(s):  
Environmental Conditions ◽  
Plant Material ◽  
Native Species ◽  
Forest Decline ◽  
Phytophthora Cinnamomi ◽  
Castanea Sativa ◽  
Forest Species ◽  
Living Root ◽  
Root Tissues ◽  
Ink Disease

Several forest species are severely affected by Phytophthora cinnamomi. The contribution of this oomycete to forest decline and dieback has been broadly reported. In particular, it is consensual that it is the causal agent of ink disease in Castanea sativa. It has been associated with the severe decline of Quercus species, namely the Q. suber and Q. ilex dieback in Portugal and Spain, and has been responsible for the infection of numerous native species and crops. This pathogen persists in the soil or on plant material in the form of chlamydospores allowing the infection of living root tissues when environmental conditions are favorable.

scholarly journals A New Clonal Propagation Protocol Develops Quality Root Systems in Chestnut

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 826
Author(s):  
Patrícia Fernandes ◽  
Sara Tedesco ◽  
Inês Vieira da Silva ◽  
Carmen Santos ◽  
Helena Machado ◽  
...  
Keyword(s):  
Clonal Propagation ◽  
Phytophthora Cinnamomi ◽  
Castanea Sativa ◽  
Breeding Programs ◽  
Continuous Growth ◽  
European Chestnut ◽  
High Incidence ◽  
Castanea Sativa Mill ◽  
Large Production

There is, at the present time, a great demand for chestnut rootstocks with improved resistance to Phytophthora cinnamomi Rands in the nurseries. New genotypes are emerging from European chestnut breeding programs and the production of thriving plants to restore old orchards with low yields due to a high incidence of diseases, namely root rot, is necessary. Micropropagation is a useful technique for clonal propagation. Nevertheless, in vitro culture propagation is genotype-dependent. Consequently, the existing protocols may demonstrate poor reproducibility and low efficacy. Thus, the need to contribute to the development of new micropropagation protocols suitable for large production of emerging genotypes. As a contribution to fill this gap, a three-step protocol was developed by using new combinations of Murashige & Skoog, Woody Plant, and adapted modified Melin-Norkrans media in different stages of the propagation process. About 90% of shoots were rooted, and after three months of acclimatization, 85% of these plants survived and were capable of continuous growth in the field. Currently, this protocol is being used in the production of several hybrid genotypes (with improved resistance to P. cinnamomi), selected from our ongoing breeding program and also in Castanea sativa Mill. and Castanea crenata Siebold and Zucc. species.

scholarly journals The rbcL gene sequence from chestnut indicates a slow rate of evolution in the Fagaceae

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 668-671 ◽  
Author(s):  
Nathalie Frascaria ◽  
Laurent Maggia ◽  
Martin Michaud ◽  
Jean Bousquet
Keyword(s):  
Gene Sequence ◽  
Large Subunit ◽  
Castanea Sativa ◽  
Rbcl Gene ◽  
Bisphosphate Carboxylase ◽  
Reading Frame ◽  
Rate Of Evolution ◽  
Gene Coding ◽  
The Family ◽  
Castanea Sativa Mill

The nucleotide sequence was obtained for the chloroplast gene coding for the large subunit of the ribulose 1,5-bisphosphate carboxylase (rbcL) of chestnut (Castanea sativa Mill.), a member of the woody family Fagaceae. Amplification primers downstream and upstream the rbcL open reading frame are also described. By comparing with other angiosperm sequences, we show that the rate of evolution of rbcL in the family Fagaceae is much slower than that observed for the families of annuals analyzed.Key words: angiosperms, Castanea sativa, Fagaceae, phylogeny, rbcL.

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