Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis

The aim of the present work was to characterize changes in the protein profile throughout seed development in O. catharinensis, a recalcitrant species, by two-dimensional gel electrophoresis. Protein extraction was undertaken by using a thiourea/urea buffer, followed by a precipitation step with 10% TCA. Comparative analysis during seed development showed that a large number of proteins were exclusively detected in each developmental stage. The cotyledonary stage, which represents the transition phase between embryogenesis and the beginning of metabolism related to maturation, presents the highest number of stage-specific spots. Protein identification, through MS/MS analysis, resulted in the identification of proteins mainly related to oxidative metabolism and storage synthesis. These findings contribute to a better understanding of protein metabolism during seed development in recalcitrant seeds, besides providing information on established markers that could be useful in defining and improving somatic embryogenesis protocols, besides monitoring the development of somatic embryos in this species.

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Analysis of proteins associated with storage root formation in cassava using two-dimensional gel electrophoresis

Revista Brasileira de Fisiologia Vegetal ◽

10.1590/s0103-31312001000100005 ◽

2001 ◽

Vol 13 (1) ◽

pp. 41-48 ◽

Cited By ~ 8

Author(s):

GLAUCIA B. CABRAL ◽

LUIZ J.C.B. CARVALHO

Keyword(s):

Gel Electrophoresis ◽

Adventitious Root ◽

Protein Pattern ◽

Protein Profile ◽

Secondary Growth ◽

Starch Accumulation ◽

Computer Assisted ◽

Two Dimensional ◽

Storage Root ◽

Two Dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis analysis was performed on adventitious and storage root in cassava (Manihot esculenta Crantz). Adventitious root lacking swelling formation and swelled storage root were obtained from the accession WU104 grown in the green house of the Department of Biology in Washington University in St. Louis (MO-USA). Saline buffer-soluble proteins were extracted, separated in a high-resolution 2-D electrophoresis system, visualized with silver staining gel procedure, and digital image generate for further analysis. Quantitative and qualitative protein spots analysis was performed with a computer assisted image software system. Results revealed large variation in the complexity of the gel protein profile between the two root systems. About 90% of the protein spots appeared in the pI range value of 4.0 to 6.5 and between 14 to 80 Kda of molecular mass. Detailed computer assisted analysis of this gel allowed us to establish 5 distinct classes of protein based on spot quantification that could be associated with swelling and non-swelling roots. Variation in the complexity of protein pattern was related with different type of root. Whereas the adventitious root showed a more simple profile related to primary growth, the storage root showed to be a more complex profile related to secondary growth and starch accumulation.

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Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2018.710.407 ◽

2018 ◽

Vol 7 (10) ◽

pp. 3524-3531

Author(s):

Nimita Umretiya ◽

Hitesh Jasani ◽

Nilam Tulsani ◽

Abhijeeta Nandha ◽

B.A. Golakiya

Keyword(s):

Arachis Hypogaea ◽

Flower Development ◽

Gel Electrophoresis ◽

Protein Profile ◽

Development Stage ◽

Two Dimensional ◽

Two Dimensional Gel Electrophoresis

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Study of Sperm Protein Profile in Men With and Without Varicocele Using Two-Dimensional Gel Electrophoresis

Urology ◽

10.1016/j.urology.2012.06.027 ◽

2013 ◽

Vol 81 (2) ◽

pp. 293-300 ◽

Cited By ~ 32

Author(s):

Hani Hosseinifar ◽

Hamid Gourabi ◽

Ghasem Hosseini Salekdeh ◽

Mehdi Alikhani ◽

Shahab Mirshahvaladi ◽

...

Keyword(s):

Gel Electrophoresis ◽

Protein Profile ◽

Two Dimensional ◽

Two Dimensional Gel Electrophoresis ◽

Sperm Protein

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Protein Profile Analysis of Two Australian Snake Venoms by One- Dimensional Gel Electrophoresis and MS/MS Experiments

Current Medicinal Chemistry ◽

10.2174/0929867324666170601073148 ◽

2017 ◽

Vol 24 (17) ◽

Cited By ~ 1

Author(s):

Dessislava Georgieva ◽

Diana Hildebrand ◽

Rodrigo Simas ◽

Monika A. Coronado ◽

Marcel Kwiatkowski ◽

...

Keyword(s):

Gel Electrophoresis ◽

Profile Analysis ◽

Protein Profile ◽

Snake Venoms ◽

One Dimensional

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A multifaceted analysis of viperid snake venoms by two-dimensional gel electrophoresis: An approach to understanding venom proteomics

PROTEOMICS ◽

10.1002/pmic.200400931 ◽

2005 ◽

Vol 5 (2) ◽

pp. 501-510 ◽

Cited By ~ 109

Author(s):

Solange M. T. Serrano ◽

John D. Shannon ◽

Deyu Wang ◽

Antonio C. M. Camargo ◽

Jay W. Fox

Keyword(s):

Gel Electrophoresis ◽

Two Dimensional ◽

Snake Venoms ◽

Two Dimensional Gel Electrophoresis ◽

Multifaceted Analysis

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Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660085 ◽

1985 ◽

Vol 54 (03) ◽

pp. 626-629 ◽

Cited By ~ 4

Author(s):

M Meyer ◽

F H Herrmann

Keyword(s):

High Resolution ◽

Gel Electrophoresis ◽

Type Ii ◽

Two Dimensional ◽

Two Dimensional Gel Electrophoresis ◽

Structural Variant ◽

Glanzmann’S Thrombasthenia ◽

Crossed Immunoelectrophoresis ◽

Glanzmann's Thrombasthenia ◽

Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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