scholarly journals Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage

2018 ◽  
Vol 7 (10) ◽  
pp. 3524-3531
Author(s):  
Nimita Umretiya ◽  
Hitesh Jasani ◽  
Nilam Tulsani ◽  
Abhijeeta Nandha ◽  
B.A. Golakiya
Keyword(s):  
Arachis Hypogaea ◽  
Flower Development ◽  
Gel Electrophoresis ◽  
Protein Profile ◽  
Development Stage ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Analysis of proteins associated with storage root formation in cassava using two-dimensional gel electrophoresis

2001 ◽  
Vol 13 (1) ◽  
pp. 41-48 ◽  
Author(s):  
GLAUCIA B. CABRAL ◽  
LUIZ J.C.B. CARVALHO
Keyword(s):  
Gel Electrophoresis ◽  
Adventitious Root ◽  
Protein Pattern ◽  
Protein Profile ◽  
Secondary Growth ◽  
Starch Accumulation ◽  
Computer Assisted ◽  
Two Dimensional ◽  
Storage Root ◽  
Two Dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis analysis was performed on adventitious and storage root in cassava (Manihot esculenta Crantz). Adventitious root lacking swelling formation and swelled storage root were obtained from the accession WU104 grown in the green house of the Department of Biology in Washington University in St. Louis (MO-USA). Saline buffer-soluble proteins were extracted, separated in a high-resolution 2-D electrophoresis system, visualized with silver staining gel procedure, and digital image generate for further analysis. Quantitative and qualitative protein spots analysis was performed with a computer assisted image software system. Results revealed large variation in the complexity of the gel protein profile between the two root systems. About 90% of the protein spots appeared in the pI range value of 4.0 to 6.5 and between 14 to 80 Kda of molecular mass. Detailed computer assisted analysis of this gel allowed us to establish 5 distinct classes of protein based on spot quantification that could be associated with swelling and non-swelling roots. Variation in the complexity of protein pattern was related with different type of root. Whereas the adventitious root showed a more simple profile related to primary growth, the storage root showed to be a more complex profile related to secondary growth and starch accumulation.

Protein Profile analysis of HeLa Nuclear and Cytoplasmic Subcellular Fractions by Two Dimensional Gel Electrophoresis

2018 ◽  
Vol 15 (3) ◽  
pp. 221-228
Author(s):  
Esteban Velasquez Saldarriaga ◽  
Johanna C. Arroyave Ospina ◽  
Johanna Pedroza-Díaz ◽  
Fabian M. Cortes-Mancera
Keyword(s):  
Gel Electrophoresis ◽  
Profile Analysis ◽  
Protein Profile ◽  
Subcellular Fractions ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Study of Sperm Protein Profile in Men With and Without Varicocele Using Two-Dimensional Gel Electrophoresis

Urology ◽  
2013 ◽  
Vol 81 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Hani Hosseinifar ◽  
Hamid Gourabi ◽  
Ghasem Hosseini Salekdeh ◽  
Mehdi Alikhani ◽  
Shahab Mirshahvaladi ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Protein Profile ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Sperm Protein

scholarly journals Polypeptide Composition of Arachin and Non-arachin Proteins From Early Bunch Peanut (Arachis hypogaea L.) Seed1

1981 ◽  
Vol 8 (2) ◽  
pp. 82-88 ◽  
Author(s):  
Shaik-M. M. Basha ◽  
Sunil K. Pancholy
Keyword(s):  
Molecular Weight ◽  
Arachis Hypogaea ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Seed Proteins ◽  
Two Dimensional ◽  
Polypeptide Composition ◽  
Two Dimensional Gel Electrophoresis ◽  
Arachis Hypogaea L ◽  
Isoelectric Points

Abstract Peanut (Arachis hypogaea L.) seed proteins were resolved into arachin and non-arachin fractions, and composite two-dimensional polypeptide maps were prepared. Seed proteins were extracted with a buffer containing 2 M NaCl, 10 mM Tris-HCl (pH 8.2), 0.2 mM phenylmethyl sulfonyl fluoride and 0.002% NaN3 and resolved into ten peaks by gel filtration on a Sephacryl S-300 column. Gel filtration of total protein extract yielded three molecular weight variants (490,000., 400,000, and 365,000) of arachin. Gel electrophoresis showed quantitative and qualitative differences in the protein and polypeptide composition of the three arachin variants. Nonarachin proteins obtained by this method were heterogeneous and distinct from the arachin. Two-dimensional gel electrophoresis revealed several differences in the polypeptide composition between arachin fraction IV and fractions II and III. Composite two-dimensional polypeptide maps of arachin and non-arachin revealed the presence of several polypeptides with similar isoelectric points and molecular weights between them. Arachin contained six molecular weight (between 15,500 and 68,000) classes of polypeptides with isoelectric points between 4.7 and 8.4 while nonarachin contained nine molecular weight (between 16,000 and 170,000) classes of polypeptides having isoelectric points between 4.7 and 7.9.

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range

2015 ◽  
Vol 22 (12) ◽  
pp. 1066-1075 ◽  
Author(s):  
Adriana Magalhães ◽  
Rayner Queiroz ◽  
Izabela Bastos ◽  
Jaime Santana ◽  
Marcelo Sousa ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Alkaline Ph ◽  
Two Dimensional Gel Electrophoresis ◽  
Ph Range

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

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