Molecular Phylogeny of the Cuscuta californica Complex (Convolvulaceae) and a New Species from New Mexico and Trans-Pecos

2009 ◽  
Vol 34 (3) ◽  
pp. 570-579 ◽  
Author(s):  
Mihai Costea ◽  
Saša Stefanović

Multiple DNA sequences from plastid (trnL–F region and rbcL) as well as nuclear (ITS and 26S rDNA) genomes were used to infer the phylogeny of the Cuscuta californica complex. This group is currently circumscribed to include nine species distributed mostly in western North America. Four well-supported lineages have been revealed within this complex. The first lineage includes the controversial C. californica s. l., an assemblage of taxa characterized by their lack of infrastaminal scales; the second lineage consists of a single species, C. subinclusa, with short fimbriate scales. The third lineage groups C. howelliana, C. salina, and C. suksdorfii, with scales that exhibit a reduction trend, while the forth includes C. decipiens and a new species from New Mexico and trans-Pecos Texas, C. draconella, both with well-developed infrastaminal scales. Stereo, compound, and scanning electron microscopy were used to investigate the new species and compare it with C. decipiens, its closest relative, as well as to update the taxonomic treatment of C. californica s. l. Cuscuta decipiens, in its original delimitation, is polyphyletic and was thus recircumscribed. In contrast to previous taxonomic treatments of C. californica s. l., phylogenetic relationships in conjunction with morphological data support the delimitation of three species: C. brachycalyx, C. californica, and C. occidentalis.

Phytotaxa ◽  
2019 ◽  
Vol 391 (2) ◽  
pp. 122 ◽  
Author(s):  
MURAT KOÇ ◽  
ERGIN HAMZAOĞLU ◽  
AHMET AKSOY

The genus Minuartia is represented in Turkey by 34 taxa. Some interesting specimens were collected from Antalya province, and examined. These specimens resemble Minuartia meyeri, and M. multinervis from which differ by characters (macro-, and micromorphological) of inflorescence, alar pedicels, petals, sepals, capsules and seeds. Moreover, by using the DNA sequences of the ITS genes, phylogenetic relationships between this collected species, and the related species were investigated. As a result of the evaluation of molecular, and morphological data, we proposed to described the population from Antalya as a new species for the science. A description, pictures, distribution, habitat, and IUCN category are given.


Zootaxa ◽  
2013 ◽  
Vol 3616 (2) ◽  
pp. 135-150 ◽  
Author(s):  
HELEN K. LARSON ◽  
RALPH FOSTER ◽  
WILLIAM F. HUMPHREYS ◽  
MARK I. STEVENS

A new species of the eyeless eleotrid genus Milyeringa is described from wells sunk on Barrow Island, Western Australia. Milyeringa justitia n. sp. is the third species of the genus to be named. Morphological data and cytochrome c oxidase subunit I (COI) DNA sequence data from a wide sample of localities at which the genus occurs was used to evaluate relationships and species limits. Milyeringa veritas is redescribed, and M. brooksi is synonymised with M. veritas. The unique form and ecology of these fishes, plus the threats to their survival, warrants immediate and continuing attention in management.


Phytotaxa ◽  
2020 ◽  
Vol 429 (4) ◽  
pp. 281-288
Author(s):  
LIANG MA ◽  
SAI ZHANG ◽  
ZHUANG ZHOU ◽  
SI-REN LAN ◽  
SHI-PIN CHEN

We have described and illustrated a new species, Bulbophyllum linzhiense (Malaxideae, Epidendroideae, Orchidaceae), from Tibet Autonomous Region in southwest China. The size and overall floral morphology of the new species are similar to those of B. rubrolabellum, a species endemic to Taiwan island on the southeast coast of China, but is different by apart pseudobulbs, lower flowers, ovate-lanceolate bracts, longer pedicel and ovary, yellow-green flower, and lanceolate lateral sepals. A cladistic analysis based on the nuclear (ITS) and plastid (matK, trnL-F, and atpI-atpH) DNA sequences supported the new species is sister to B. rubrolabellum.


Phytotaxa ◽  
2021 ◽  
Vol 500 (3) ◽  
pp. 217-224
Author(s):  
SHUAI PENG ◽  
YI-YAN CONG ◽  
JING TIAN ◽  
CAI-FEI ZHANG ◽  
GUANG-WAN HU ◽  
...  

Impatiens bullatisepala (Balsaminaceae), a new species supported by morphological and phylogenetic evidence from Fanjing Mountain, Guizhou province in China, is described here. It is morphologically similar to I. davidii but can be distinguished by its dorsally ridged lateral sepals with sunk reticulate veins and bullate projections on abaxial surface, 2–2.5 cm deep saccate lower sepal with ca. 0.8 cm long narrowly triangular tip at the mouth, and broadly ovate dorsal petal. Phylogenetic analyses of a combined dataset of nuclear ITS and plastid atpB-rbcL intergenic spacer DNA sequences furtherly confirmed its novelty.


Phytotaxa ◽  
2017 ◽  
Vol 296 (2) ◽  
pp. 171 ◽  
Author(s):  
SAROJ RUCHISANSAKUN ◽  
PIYAKASET SUKSATHAN ◽  
TIMOTHEÜS VAN DER NIET ◽  
SAW LWIN ◽  
STEVEN B. JANSSENS

Impatiens tanintharyiensis Ruchisansakun, Suksathan & Saw-Lwin from the Tanintharyi region of Southern Myanmar is described and illustrated as a new species. The presence of connate lateral united petals and a four-locular ovary, as well as results of molecular phylogenetic analyses of nuclear ITS and plastid atpB-rbcL spacer DNA sequences, suggest that the new species is a member of Impatiens section Semeiocardium (Zoll.) S.X. Yu & Wei Wang. The new species is morphologically most similar to I. spectabilis Triboun & Suksathan, but can be distinguished by its asymmetric flowers, saccate-bucciniform lower sepal, and shorter, slightly incurved spur. Floral traits, including the presence of a large floral chamber with a wide entrance, are consistent with the bee-pollination syndrome in Impatiens. Since I. tanintharyiensis is only known from two small populations, its conservation status is assessed as Endangered.


Zootaxa ◽  
2018 ◽  
Vol 4377 (1) ◽  
pp. 39 ◽  
Author(s):  
VALENTIN DE MAZANCOURT ◽  
GERARD MARQUET ◽  
D. CHRISTOPHER ROGERS ◽  
PHILIPPE KEITH

During field trips to Guam and Babeldaob Islands in Micronesia, freshwater shrimps were sampled and assigned either to Caridina brachydactyla De Man, 1908 or C. mertoni J. Roux, 1911 (Decapoda: Atyidae), following previous inventories. In combining morphological data with a genetical analysis, it appeared that all the specimens belonged to a new species, Caridina variabilis sp. nov., here described with its distribution. The status of this new species is clarified and finally, neither C. brachydactyla, nor C. mertoni occur in these two islands. DNA sequences of 16S were obtained from the syntypes of C. mertoni. 


Phytotaxa ◽  
2015 ◽  
Vol 222 (1) ◽  
pp. 33 ◽  
Author(s):  
Dai-Gui Zhang ◽  
Tao Deng ◽  
Changkyun King ◽  
Jianwen Zhang ◽  
Zelong Nie ◽  
...  

A distinctive, previously undescribed species of Epimedium (Berberidaceae), which we propose naming E. tianmenshanensis, was found during a floristic survey in Hunan, China. It is similar to E. baojingense and E. franchetii, but has smaller flowers and petals with a short, blunt spur. We determined the phylogenetic position of the new species using DNA sequences of nuclear ITS and two chloroplast regions (atpB-rbcL intergenic spacer and matK). Molecular evidence indicates that E. tianmenshanensis occupies a distinct group related to E. baojingense.


ZooKeys ◽  
2020 ◽  
Vol 923 ◽  
pp. 115-140 ◽  
Author(s):  
Tao Luo ◽  
Ning Xiao ◽  
Kai Gao ◽  
Jiang Zhou

This study describes a new species of the genus Leptobrachella, Leptobrachella suiyangensissp. nov. from the Huoqiuba Nature Reserve, Suiyang County, Guizhou Province, China, based on morphological data and phylogenetic analyses (16S rRNA mtDNA). The new species can be distinguished from other congeners by the molecular divergence and by a combination of morphological characters, including body size, dorsal and ventral patterns, dorsal skin texture, size of the pectoral and femoral glands, degree of webbing and fringing on the toes and fingers, dorsum coloration, and iris coloration in life. Currently, the genus Leptobrachella contains 75 species, 21 of which are found in China, including seven species reported from Guizhou Province. The uncorrected sequence divergence percentage between Leptobrachella suiyangensissp. nov. and all homologous DNA sequences available for the 16S rRNA gene was found to be >4.7%. The new record of the species and its relationships with others in the same genus imply that species distribution, habitat variation, environmental adaptation, and diversity of the genus Leptobrachella in southwest China need to be further investigated.


PhytoKeys ◽  
2019 ◽  
Vol 135 ◽  
pp. 59-69
Author(s):  
Nan Lin ◽  
Dai-Gui Zhang ◽  
Xian-Han Huang ◽  
Jian-Wen Zhang ◽  
Jing-Yuan Yang ◽  
...  

Silene sunhangii, a new species of Caryophyllaceae known from only three populations in Hubei and Hunan provinces of central China, is described. Both morphological and molecular data were used to assess the taxonomic status and relationships of this species. Morphologically, S. sunhangii is most similar to S. platyphylla Franch. from which it differs most readily in having 3-veined elliptical leaves without pubescence, tasseled catacorolla, pale purple to red petals without a linear lobe or narrow tooth and lanceolate, bifid to one third. A phylogenetic analysis based on nuclear ITS region identified the new species as a well-supported, independent lineage. Our new species is nested within a grade that encompasses species representing a polyphyletic Silene sect. Physolychnis (Benth.) Bocquet. Both the genetic and morphological data support the recognition of Silene sunhangii as a distinct species, although there is inconsistency between these two datasets as to the relationships of the new species.


ZooKeys ◽  
2019 ◽  
Vol 817 ◽  
pp. 131-157 ◽  
Author(s):  
John C. Murphy ◽  
Alvin L. Braswell ◽  
Stevland P. Charles ◽  
Renoir J. Auguste ◽  
Gilson A. Rivas ◽  
...  

Tobago is a small island on the southeast edge of the Caribbean Plate with a continental flora and fauna. Using DNA sequences from Genbank, new sequences, and morphological data from the snakesErythrolamprusepinephalus,E.melanotus,E.reginae, andE.zweifeli, the species status of specimens of a Tobago snake previously considered to beErythrolamprusreginaewas assessed.Erythrolampruszweifeli, long considered a subspecies ofE.reginae, was found to be a northern Venezuela-Trinidad endemic and the sister toE.reginae. The trans-Andean speciesE.epinephalusis shown to be non-monophyletic while the Costa Rican lineage ofE.epinephalusis weakly supported as the sister to the Tobago population. The TobagoErythrolamprusis described as a distinct taxon based upon five specimens from four localities in lower montane rainforest. Much of the new species range includes the Main Ridge Forest Reserve of Tobago, the oldest protected forest in the Western Hemisphere. All known locations fall within a 400-ha area, and its total geographic distribution is likely to be less than 4,566 ha. The restricted distribution of this new snake makes it a likely candidate for threatened status. The new species also becomes another biogeographic link between northern Venezuela and Tobago.


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