Aeschynomene chicocesariana, a Striking New Unifoliolate Legume Species from the Brazilian Chapada Diamantina and its Phylogenetic Placement in the Dalbergioid Clade

Abstract—Unifoliolate leaves are relatively rare in the species-rich dalbergioid clade of papilionoid legumes (Leguminosae, Papilionoideae). For the pantropical, diverse genus Aeschynomene (ca. 180 species), the only known record of unifoliolate species described so far is the savanna-inhabiting A. simplicifolia from Chapada dos Veadeiros in Goiás, Brazil. The current work provides a description, illustrations, and a distribution map of another unifoliolate species, Aeschynomene chicocesariana, which was newly discovered in the rupestrian grasslands of Chapada Diamantina in Bahia, Brazil. Bayesian phylogenetic analyses of DNA sequence data (nuclear ribosomal ITS/5.8S and the plastid genes matK and trnL intron) have unequivocally placed A. chicocesariana with the rest of Aeschynomene species that are traditionally placed within Aeschynomene sect. Ochopodium. This result agrees with the shared morphology of A. chicocesariana and members of that section.

Download Full-text

Phylogenetic placement and a new circumscription of Poteranthera (Microlicieae; Melastomataceae)

Phytotaxa ◽

10.11646/phytotaxa.263.3.3 ◽

2016 ◽

Vol 263 (3) ◽

pp. 219 ◽

Cited By ~ 8

Author(s):

MARIA JOSÉ REIS DA ROCHA ◽

PAULO JOSÉ FERNANDES GUIMARÃES ◽

FABIÁN A. MICHELANGELI ◽

ROSANA ROMERO

Keyword(s):

South America ◽

Dna Sequence ◽

Sequence Data ◽

Phylogenetic Analyses ◽

New Combination ◽

Phylogenetic Position ◽

Glandular Hairs ◽

Dna Sequence Data ◽

Phylogenetic Placement ◽

Generic Description

Poteranthera (Melastomataceae) is a genus of small, annual herbs with narrow leaves, that inhabit humid savannas in South America and are visible only when in bloom. Due to their size and paucity of collections, the phylogenetic position of Poteranthera has been uncertain to this point. Poteranthera has been at times associated, confused, or placed in Acisanthera and/or Siphanthera. Phylogenetic analyses based on DNA sequence data place Poteranthera unequivocally within the Microlicieae and not closely related to Acisanthera or Siphantera. These results are consistent with seed and anther morphology. Putative morphological synapomorphies for this genus are the glandular hairs restricted to the leaf margins and the apically constricted hypanthium. Based on morphology, Acisanthera leptalea seems better placed in Poteranthera and the new combination Poteranthera leptalea is made. An expanded generic description is also provided.

Download Full-text

Morphological and molecular evidence places Maronina into synonymy with Protoparmelia (Ascomycota: Lecanorales)

The Lichenologist ◽

10.1017/s0024282911000284 ◽

2011 ◽

Vol 43 (6) ◽

pp. 561-567 ◽

Cited By ~ 15

Author(s):

K. PAPONG ◽

G. KANTVILAS ◽

H. T. LUMBSCH

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Distinct Species ◽

Genetic Distances ◽

Molecular Evidence ◽

New Combinations ◽

Secondary Chemistry ◽

Phenotypic Characters ◽

Dna Sequence Data ◽

Phylogenetic Placement

AbstractThe phylogenetic placement of the genus Maronina was studied, based chiefly on phenotypic characters such as thallus colour and anatomy, secondary chemistry, the anatomy of the excipulum and the ascus-type. DNA sequence data of mitochondrial and nuclear ribosomal loci from some of the species support the hypothesis that Maronina is nested within Protoparmelia. Hence, Maronina is reduced to synonymy with Protoparmelia. Comparison of genetic distances suggests that the two varieties within M. orientalis should be regarded as distinct species. Consequently, the new combinations Protoparmelia australiensis (Hafellner & R. W. Rogers) Kantvilas et al., P. corallifera (Kantvilas & Papong) Kantvilas et al., P. hesperia (Kantvilas & Elix) Kantvilas et al., P. multifera (Nyl.) Kantvilas et al., and P. orientalis (Kantvilas & Papong) Kantvilas et al. are proposed.

Download Full-text

Molecular phylogenetics of the Onobrychis genus (Fabaceae: Papilionoideae) using ITS and trnL–trnF DNA sequence data

Australian Journal of Botany ◽

10.1071/bt13279 ◽

2014 ◽

Vol 62 (3) ◽

pp. 235 ◽

Cited By ~ 2

Author(s):

S. Safaei Chaei Kar ◽

F. Ghanavati ◽

M. R. Naghavi ◽

H. Amirabadi-zade ◽

R. Rabiee

Keyword(s):

Dna Sequence ◽

Molecular Phylogenetics ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Dna Sequence Data ◽

Group I ◽

Molecular Phylogenetic ◽

Nuclear Its ◽

Cultivated Species ◽

Relationship Of

Onobrychis, comprising more than 130 species, is a genus of the family Fabaceae. At this time, the interspecies relationship of this biologically important genus is still a subject of great discussion and debate. To help resolve this disagreement, we used molecular phylogeny to analyse internal transcribed spacer (ITS) and trnL–trnF sequences of 76 species of Onobrychis. Bayesian interference, maximum parsimony and maximum likelihood analyses of nuclear ITS and plastid trnL–trnF DNA sequence data generated trees with strong posterior probability for two groups: Onobrychis subgen. Sisyrosema (including: Heliobrychis, Hymenobrychis, Afghanicae and Anthyllium sections) along with Laxiflorae section in Group I and Onobrychis subgen. Onobrychis (except Laxiflorae section) in the other (Group II). The Laxiflorae section roots back to the ancestral node for Sisyrosema subgen. O. viciifolia (cultivated species), which is closely associated with O. cyri var. cyri, suggesting that the latter may be a wild progenitor of O. viciifolia. The present study supported the paraphyly of subgenera Onobrychis and Sisyrosema. The study proposed the paraphyletic nature of the sections Onobrychis, Dendrobrychis, Heliobrychis and Hymenobrychis. Together with our molecular phylogenetic analyses we present a review of Onobrychis morphology and discuss and compare our results with those of earlier morphological and molecular phylogenetic analyses.

Download Full-text

Veterinary Fusarioses within the United States

Journal of Clinical Microbiology ◽

10.1128/jcm.01607-16 ◽

2016 ◽

Vol 54 (11) ◽

pp. 2813-2819 ◽

Cited By ~ 21

Author(s):

Kerry O'Donnell ◽

Deanna A. Sutton ◽

Nathan Wiederhold ◽

Vincent A. R. G. Robert ◽

Pedro W. Crous ◽

...

Keyword(s):

United States ◽

Dna Sequence ◽

Species Complex ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Distinct Species ◽

The United States ◽

Content Type ◽

Dna Sequence Data ◽

Species Complexes

Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.

Download Full-text

Senecio imbaburensis, proposed name for Lasiocephalus sodiroi in the genus Senecio (Asteraceae)

Phytotaxa ◽

10.11646/phytotaxa.152.1.8 ◽

2013 ◽

Vol 152 (1) ◽

pp. 59

Author(s):

Karol Marhold ◽

Petr Sklenář

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Plastid Dna ◽

Dna Sequence Data ◽

Number Of Species ◽

The Andes

Lasiocephalus Willd. ex Schlechtendal (1818: 308), as traditionally circumscribed (e.g., by Cuatrecasas 1978, Dušková et al. 2010), is a neotropical genus of ca 25 species confined to the Andes and distributed from Venezuela to Bolivia. Nevertheless, recent studies by Pelser et al. (2007, 2010) have shown that based on phylogenetic analyses of nrITS and plastid DNA sequence data, species of the genus Lasiocephalus are deeply embedded in Senecio Linnaeus (1753: 866), and, consequently, should be transferred into this latter genus. In fact, a number of species of Lasiocephalus were originally described as Senecio or had been, at some point, transferred into Senecio so only few transfers are necessary.

Download Full-text

Is Ronnbergia (Bromeliaceae, Bromelioideae) a geographically disjunct genus? Evidence from morphology and chloroplast DNA sequence data

Phytotaxa ◽

10.11646/phytotaxa.219.3.6 ◽

2015 ◽

Vol 219 (3) ◽

pp. 261 ◽

Cited By ~ 7

Author(s):

Julian Aguirre-Santoro ◽

Julio Betancur ◽

Gregory K. Brown ◽

Timothy M. Evans ◽

Fabiano Salgueiro ◽

...

Keyword(s):

Chloroplast Dna ◽

Dna Sequence ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Phylogenetic Reconstruction ◽

Morphological Data ◽

Phylogenetic Study ◽

Geographic Ranges ◽

Dna Sequence Data ◽

Disjunct Genus

A phylogenetic study testing the monophyly of the geographically disjunct genus Ronnbergia (Bromeliaceae, Bromelioideae) is presented. The phylogenetic analyses were based on taxon sampling that included all but one species of Ronnbergia, and representative lineages across the subfamily Bromelioideae. Three chloroplast DNA sequence markers (matK, psbA-trnH, and trnL-trnF) and morphological data were used for the phylogenetic reconstruction. Both the molecular and morphological datasets supported the polyphyly of Ronnbergia, either independently or in combination. These findings suggest that the geographic disjunction of this genus is most likely a product of taxonomic misinterpretation. The results also indicate that the species currently circumscribed in Ronnbergia are closely related to species in the genus Aechmea with similar geographic ranges. The datasets do not have enough resolution power to reconstruct a deep phylogenetic history that involves all the species of Ronnbergia. Nevertheless, this study provides clues for future approaches that should focus on a larger species sampling and the use of multi-locus DNA sequence data.

Download Full-text

Cymbidium yunnanensis: a new orchid species (Orchidaceae; Epidendroideae) from China based on morphological and molecular evidence

Phytotaxa ◽

10.11646/phytotaxa.387.2.7 ◽

2019 ◽

Vol 387 (2) ◽

pp. 149-157 ◽

Cited By ~ 5

Author(s):

GUO-QIANG ZHANG ◽

GUI-ZHEN CHEN ◽

LI-JUN CHEN ◽

SI-REN LAN

Keyword(s):

New Species ◽

Dna Sequence ◽

Yunnan Province ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Evidence ◽

Orchid Species ◽

Molecular Analyses ◽

Dna Sequence Data ◽

A New Species

A new orchid species, Cymbidium yunnanensis (Orchidaceae; Epidendroideae; Cymbidieae), from Yunnan Province, China, is described and illustrated. Based on morphological and molecular analyses, we demonstrate that this new species is similar to C. floribundum, but it differs in its smaller size, leaves 5–15 cm, shorter scape, white flowers with pale purplish markings on its sepals and petals, lip with purplish red blotch, petals narrowly falcate oblong and lip midlobe nearly round with column foot 1.5–2.0 mm long. Phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) DNA sequence data support C. yunnanensis as a new species.

Download Full-text

Culture experiments and DNA sequence data confirm the identity of Lobaria photomorphs

Canadian Journal of Botany ◽

10.1139/b03-027 ◽

2003 ◽

Vol 81 (3) ◽

pp. 232-247 ◽

Cited By ~ 37

Author(s):

Soili Stenroos ◽

Elfriede Stocker-Wörgötter ◽

Isao Yoshimura ◽

Leena Myllys ◽

Arne Thell ◽

...

Keyword(s):

Dna Sequence ◽

Green Alga ◽

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Its Rdna ◽

Dna Sequence Data

Lichens consist of a fungal component that associates with either a green alga or cynobacterium, resulting in diverse morphologies. Resynthesis experiments of isolated myco- and photobionts, as well as internal transcribed spacer (ITS) rDNA phylogenies, suggest that the notoriously dissimilar photomorphs of Lobaria fendleri (Tuck. ex Mont.) Lindau and Lobaria amplissima (Scop.) Forss. both have a single mycobiont, which can simultaneously lichenize two unrelated photobionts, a eukaryote and a prokaryote. In the phylogenetic analyses, the genera Sticta and Durietzia appear monophyletic; whereas Lobaria and Pseudocyphellaria are both nonmonophyletic. Furthermore, the phylogenetic relationships of Durietzia, Lobarina, and Sticta relative to Lobaria and Pseudocyphellaria are in need of re-evaluation.Key words: internal transcribed spacer (ITS), photosymbiodeme, phylogeny, lichen-forming fungi, Lobaria amplissima, Lobaria fendleri, Pseudocyphellaria, Sticta.

Download Full-text

Lichens or endophytes? The enigmatic genus Leptosillia in the Leptosilliaceae fam. nov. (Xylariales), and Furfurella gen. nov. (Delonicicolaceae)

Persoonia - Molecular Phylogeny and Evolution of Fungi ◽

10.3767/persoonia.2019.42.09 ◽

2019 ◽

Vol 42 (1) ◽

pp. 228-260 ◽

Cited By ~ 2

Author(s):

H. Voglmayr ◽

M.B. Aguirre-Hudson ◽

H.G. Wagner ◽

S. Tello ◽

W.M. Jaklitsch

Keyword(s):

Dna Sequence ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Rdna Sequence ◽

Lsu Rdna ◽

Tropical Regions ◽

Dna Sequence Data ◽

New Family ◽

Rdna Sequence Data ◽

Molecular Phylogenetic

Based on DNA sequence data, the genus Leptosillia is shown to belong to the Xylariales. Molecular phylogenetic analyses of ITS-LSU rDNA sequence data and of a combined matrix of SSU-ITS-LSU rDNA, rpb1, rpb2, tef1 and tub2 reveal that the genera Cresporhaphis and Liberomyces are congeneric with Leptosillia. Coelosphaeria fusariospora, Leptorhaphis acerina, Leptorhaphis quercus f. macrospora, Leptorhaphis pinicola, Leptorhaphis wienkampii, Liberomyces pistaciae, Sphaeria muelleri and Zignoëlla slaptonensis are combined in Leptosillia, and all of these taxa except for C. fusariospora, L. pinicola and L. pistaciae are epitypified. Coelosphaeria fusariospora and Cresporhaphis rhoina are lectotypified. Liberomyces macrosporus and L. saliciphilus, which were isolated as phloem and sapwood endophytes, are shown to be synonyms of Leptosillia macrospora and L. wienkampii, respectively. All species formerly placed in Cresporhaphis that are now transferred to Leptosillia are revealed to be non-lichenized. Based on morphology and ecology, Cresporhaphis chibaensis is synonymised with Rhaphidicyrtis trichosporella, and C. rhoina is considered to be unrelated to the genus Leptosillia, but its generic affinities cannot be resolved in lack of DNA sequence data. Phylogenetic analyses place Leptosillia as sister taxon to Delonicicolaceae, and based on morphological and ecological differences, the new family Leptosilliaceae is established. Furfurella, a new genus with the three new species, F. luteostiolata, F. nigrescens and F. stromatica, growing on dead branches of mediterranean fabaceous shrubs from tribe Genisteae, is revealed to be the closest relative of Delonicicola in the family Delonicicolaceae, which is emended. ITS rDNA sequence data retrieved from GenBank demonstrate that the Leptosilliaceae were frequently isolated or sequenced as endophytes from temperate to tropical regions, and show that the genus Leptosillia represents a widely distributed component of endophyte communities of woody plants.

Download Full-text

Circinotrichum sinense, a new asexual fungus from Hubei, China

Botany ◽

10.1139/cjb-2017-0132 ◽

2017 ◽

Vol 95 (12) ◽

pp. 1099-1108 ◽

Cited By ~ 1

Author(s):

De-Wei Li ◽

Neil P. Schultes ◽

Jing-Yuan Chen ◽

Yi-Xun Wang ◽

Rafael Felipe Castañeda-Ruiz

Keyword(s):

Dna Sequence ◽

Nature Reserve ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

Dna Sequence Data ◽

Fungal Taxon ◽

National Nature Reserve

A setose hyphomycete was collected as part of a recent expedition to search for microfungi in the Duheyuan National Nature Reserve in Hubei, China. The conidia are typical of Circinotrichum Nees, being curved or falcate, single-celled, colorless, smooth with a setula at the apical end, and similar to Circinotrichum rigidum. Circinotrichum sinense has a longer setula only at the apical end and verrucose setae, while C. rigidum has a setula on both ends and smooth setae. Phylogenetic analyses using ITS and LSU DNA sequence data and examination of the morphological characters showed that this fungus cannot be identified as any previously described species of Circinotrichum. Thus, a new fungal taxon is described. A key to recognized species of Circinotrichum is also provided.

Download Full-text