Morphological and molecular evidence places Maronina into synonymy with Protoparmelia (Ascomycota: Lecanorales)

2011 ◽  
Vol 43 (6) ◽  
pp. 561-567 ◽  
Author(s):  
K. PAPONG ◽  
G. KANTVILAS ◽  
H. T. LUMBSCH
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Distinct Species ◽  
Genetic Distances ◽  
Molecular Evidence ◽  
New Combinations ◽  
Secondary Chemistry ◽  
Phenotypic Characters ◽  
Dna Sequence Data ◽  
Phylogenetic Placement

AbstractThe phylogenetic placement of the genus Maronina was studied, based chiefly on phenotypic characters such as thallus colour and anatomy, secondary chemistry, the anatomy of the excipulum and the ascus-type. DNA sequence data of mitochondrial and nuclear ribosomal loci from some of the species support the hypothesis that Maronina is nested within Protoparmelia. Hence, Maronina is reduced to synonymy with Protoparmelia. Comparison of genetic distances suggests that the two varieties within M. orientalis should be regarded as distinct species. Consequently, the new combinations Protoparmelia australiensis (Hafellner & R. W. Rogers) Kantvilas et al., P. corallifera (Kantvilas & Papong) Kantvilas et al., P. hesperia (Kantvilas & Elix) Kantvilas et al., P. multifera (Nyl.) Kantvilas et al., and P. orientalis (Kantvilas & Papong) Kantvilas et al. are proposed.

scholarly journals Liparis wenshanensis, a new orchid species from China: Evidence from morphological and molecular analyses

Phytotaxa ◽  
2015 ◽  
Vol 204 (4) ◽  
pp. 253 ◽  
Author(s):  
YONG-YU SU ◽  
YI-LANG HUANG ◽  
LI-JUN CHEN ◽  
PEI-WEN ZHANG ◽  
Zhong-Jian Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequence ◽  
Sequence Data ◽  
Detailed Comparison ◽  
Distinct Species ◽  
Systematic Position ◽  
Orchid Species ◽  
Molecular Analyses ◽  
Dna Sequence Data

A new orchid species, Liparis wenshanensis, discovered in Yunnan, China is described and illustrated in this study based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of the genus, Liparis, was conducted. The new plant is characterized by the combination of the following features: a long rachis with 45 to 55 flowers; white sepals, petals and column; a greenish lip with a purplish center; strongly recurved and revolute dorsal sepals and petals; strongly recurved, oblong lateral sepals; a cordate lip that is strongly deflexed below the middle, with a two-lobed apex and a two-lobed callus at the base; an arcuate column with a lamella extending along the center almost to the stigma, and with a pair of broad wings toward the apex. These features distinguish the new orchid from all other known species of Liparis. We proceeded to a phylogenetic analysis to ascertain the systematic position of this enigmatic species. Molecular analyses based on nuclear ribosomal ITS and plastid matK DNA sequence data supports the recognition of L. wenshanensis as a distinct species.

scholarly journals Veterinary Fusarioses within the United States

2016 ◽  
Vol 54 (11) ◽  
pp. 2813-2819 ◽  
Author(s):  
Kerry O'Donnell ◽  
Deanna A. Sutton ◽  
Nathan Wiederhold ◽  
Vincent A. R. G. Robert ◽  
Pedro W. Crous ◽  
...  
Keyword(s):  
United States ◽  
Dna Sequence ◽  
Species Complex ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Distinct Species ◽  
The United States ◽  
Content Type ◽  
Dna Sequence Data ◽  
Species Complexes

Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.

Cymbidium yunnanensis: a new orchid species (Orchidaceae; Epidendroideae) from China based on morphological and molecular evidence

Phytotaxa ◽  
2019 ◽  
Vol 387 (2) ◽  
pp. 149-157 ◽  
Author(s):  
GUO-QIANG ZHANG ◽  
GUI-ZHEN CHEN ◽  
LI-JUN CHEN ◽  
SI-REN LAN
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Yunnan Province ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Molecular Evidence ◽  
Orchid Species ◽  
Molecular Analyses ◽  
Dna Sequence Data ◽  
A New Species

A new orchid species, Cymbidium yunnanensis (Orchidaceae; Epidendroideae; Cymbidieae), from Yunnan Province, China, is described and illustrated. Based on morphological and molecular analyses, we demonstrate that this new species is similar to C. floribundum, but it differs in its smaller size, leaves 5–15 cm, shorter scape, white flowers with pale purplish markings on its sepals and petals, lip with purplish red blotch, petals narrowly falcate oblong and lip midlobe nearly round with column foot 1.5–2.0 mm long. Phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) DNA sequence data support C. yunnanensis as a new species.

Cymbidium densiflorum (Orchidaceae; Epidendroideae; Cymbidieae): a new orchid species from China based on morphological and molecular evidence

Phytotaxa ◽  
2018 ◽  
Vol 345 (1) ◽  
pp. 51 ◽  
Author(s):  
SIREN LAN ◽  
LIJUN CHEN ◽  
GUIZHEN CHEN ◽  
XINYI WU ◽  
WENHUI RAO ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Detailed Comparison ◽  
Molecular Study ◽  
Molecular Evidence ◽  
Orchid Species ◽  
Molecular Analyses ◽  
Large Plant ◽  
Dna Sequence Data

A new orchid species, Cymbidium densiflorum, from Yunnan, China, is described and illustrated here based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of Cymbidium was conducted. The new plant belongs to C. section Cymbidium and has a pendent inflorescence, thick-coriaceous flowers with two pollinia and rigid leaves, and it differs greatly from all other known species of this section by being a large plant bearing leaves with an acute, oblique apex and producing a long inflorescence with more than 30 purplish brown large flowers, in which the lateral sepals are oblique and narrowly elliptic with a tooth at the apex and narrowly ovate-elliptic petals. A molecular study based on the nuclear ribosomal ITS and plastid matK, rbcL, trnG, trnL and atpI DNA sequence data indicated that C. densiflorum is sister to C. paucifolium.

Reappraisal of the genera of Megalosporaceae (Teloschistales, Ascomycota)

2012 ◽  
Vol 25 (3) ◽  
pp. 210 ◽  
Author(s):  
G. Kantvilas ◽  
H. T. Lumbsch
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
New Combinations ◽  
Dna Sequence Data ◽  
Anatomical Data ◽  
Distinct Genus ◽  
The Status

The status of the genera of the lichen-forming fungal family Megalosporaceae is re-examined. DNA-sequence data utilising three loci and anatomical observations pertaining to the hymenium, asci and ascospores support Megaloblastenia as a distinct genus. Austroblastenia is not supported by molecular, morphological or anatomical data, which suggest that it should be subsumed within Megalospora. Consequently, the new combinations, Megalospora pauciseptata (Shirley) Kantvilas & Lumbsch and M. pupa (Sipman) Kantvilas & Lumbsch, are proposed.

scholarly journals The challenge of integrative taxonomy of rare, deep-water gastropods: the genus Exilia (Neogastropoda: Turbinelloidea: Ptychatractidae)

2020 ◽  
Vol 86 (2) ◽  
pp. 120-138
Author(s):  
Yuri I Kantor ◽  
Nicolas Puillandre ◽  
Philippe Bouchet
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Taxonomic Revision ◽  
Distinct Species ◽  
First Record ◽  
Integrative Taxonomy ◽  
Molecular Systematic ◽  
Dna Sequence Data ◽  
In The Wild ◽  
Anatomical Characters

Abstract According to a recent taxonomic revision by Kantor et al. (2001), the neogastropod genus Exilia Conrad, 1860, comprises ten mostly rare species that live at depths between 200 and 2000 m. Adult Exilia measure between 30 and 90 mm in shell length, and the genus is mostly represented in museum collections by empty shells. The abundance of this genus is low in the wild, but recent expeditions organized by the Muséum national d’Histoire naturelle have yielded several dozen specimens. These new collections include samples preserved for molecular studies. Here, we present the results of the first molecular systematic study of Exilia. Our aim was to investigate the species limits proposed by Kantor et al. (2001) on the basis of shell and anatomical characters. Analysis of DNA sequence data for the cytochrome c oxidase I gene suggests that Exilia hilgendorfi, previously considered to be a single, polymorphic and broadly distributed species, is a complex of at least six species (four of which we sequenced). Two of these species, Exilia cognata n. sp. and E. fedosovi n. sp., are described as new to science. Exilia gracilior, E. claydoni and E. prellei are resurrected from the synonymy of Exilia hilgendorfi; of these three, only the last was sequenced. Exilia vagrans is a well-defined taxon, but our molecular systematic data shows that it consists of two distinct species, which occur sympatrically off Taiwan and are strikingly similar in shell and radular morphology; due to the absence of DNA sequence data from the type locality of E. vagrans (Vanuatu), it is unclear to which of these two species the name would apply. Exilia karukera n. sp., which is conchologically very similar to E. vagrans, was discovered off Guadeloupe, represents the first record of the genus from the Atlantic. For E. elegans, which was previously known only from a single shell, we provide new data including new distributional records (South Africa and the Mozambique Channel), details of the radula and DNA sequence data.

The ‘Mediterranean’ Ramalina Panizzei North of the alps: Morphological, Chemical and rDNA Sequence Data

1997 ◽  
Vol 29 (5) ◽  
pp. 441-454 ◽  
Author(s):  
Urs Groner ◽  
Scott LaGreca
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Taxonomic Revision ◽  
Distinct Species ◽  
Dna Sequence Data ◽  
Rdna Its ◽  
Rdna Sequence Data ◽  
The Alps ◽  
The Mediterranean ◽  
A Site

AbstractRamalina panizzei De Not. is reported from Switzerland and north of the Alps for the first time. Recent collections and thalli found amongst specimens of R. fastigiata (Pers.) Ach. are described; the species is obviously not restricted to the Mediterranean. The confusion in several herbaria around this and related corticolous species, particularly R. subgeniculata Nyl. and R. fastigiata, can be traced back to imprecise original and subsequent diagnoses, all of which lack a clear species delimitation. Similarities and differences of these species are discussed. In addition, sequences from the rDNA ITS regions were determined for two individuals of R. panizzei and two of R. fastigiata, including one of each from a site where both species grow intermixed. Kimura 2-parameter genetic-distance estimates indicate that R. panizzei and R. fastigiata are as different from each other as either is from the reference species R. siliquosa (Hudson) A. L. Sm. s.l. A broad-based taxonomic revision of involved species is not possible due to the limited number of analyses, but the results demonstrate the potential for using DNA sequence data to investigate species-level questions in lichens. Based on morphology, chemistry, and DNA sequence data, R. panizzei is retained as a distinct species.

scholarly journals Three new species of Fomitiporella (Hymenochaetales, Basidiomycota) based on the evidence from morphology and DNA sequence data

MycoKeys ◽  
2018 ◽  
Vol 30 ◽  
pp. 73-89 ◽  
Author(s):  
Xiao-Hong Ji ◽  
Josef Vlasák ◽  
Xue-Mei Tian ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Large Subunit ◽  
Its Region ◽  
Morphological Characters ◽  
White Rot ◽  
Molecular Evidence ◽  
Dna Sequence Data ◽  
Three New Species

Fomitiporella austroasiana, F. mangrovei and F. vietnamensis are described and illustrated as new species based on morphological characters and molecular evidence. They have annual to perennial, mostly resupinate basidiomata with grayish fresh pores, an indistinct subiculum, lack any kind of setae, have brownish, thick-walled basidiospores, and cause a white rot. The distinctive morphological characters of the new species and their related species are discussed. Phylogenies based on the nuclear ribosomal large subunit (28S) and the nuclear ribosomal ITS region show that these three new species form three distinct lineages in the Fomitiporella clade. A key to known species of Fomitiporella is given.

Aeschynomene chicocesariana, a Striking New Unifoliolate Legume Species from the Brazilian Chapada Diamantina and its Phylogenetic Placement in the Dalbergioid Clade

2019 ◽  
Vol 44 (4) ◽  
pp. 810-817
Author(s):  
Domingos Benício Oliveira Silva Cardoso ◽  
Gustavo Ramos ◽  
Wallace Messias Barbosa São-Mateus ◽  
Luciano Paganucci de Queiroz
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Current Work ◽  
Legume Species ◽  
Distribution Map ◽  
Chapada Diamantina ◽  
Dna Sequence Data ◽  
Phylogenetic Placement

Abstract—Unifoliolate leaves are relatively rare in the species-rich dalbergioid clade of papilionoid legumes (Leguminosae, Papilionoideae). For the pantropical, diverse genus Aeschynomene (ca. 180 species), the only known record of unifoliolate species described so far is the savanna-inhabiting A. simplicifolia from Chapada dos Veadeiros in Goiás, Brazil. The current work provides a description, illustrations, and a distribution map of another unifoliolate species, Aeschynomene chicocesariana, which was newly discovered in the rupestrian grasslands of Chapada Diamantina in Bahia, Brazil. Bayesian phylogenetic analyses of DNA sequence data (nuclear ribosomal ITS/5.8S and the plastid genes matK and trnL intron) have unequivocally placed A. chicocesariana with the rest of Aeschynomene species that are traditionally placed within Aeschynomene sect. Ochopodium. This result agrees with the shared morphology of A. chicocesariana and members of that section.

Is the horned pitviper Ceratrimeresurus shenlii Liang and Liu, 2003 from China a valid Protobothrops?

2011 ◽  
Vol 32 (1) ◽  
pp. 132-135
Author(s):  
Ellen Hitschfeld ◽  
Anna Hundsdörfer ◽  
Markus Auer ◽  
Fumin Wang ◽  
Lihua Zhou ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Maximum Likelihood ◽  
Sequence Data ◽  
Isolation By Distance ◽  
Guangdong Province ◽  
Distinct Species ◽  
Cyt B ◽  
Dna Sequence Data ◽  
Phylogenetic Placement ◽  
Close Relationship

AbstractPrevious records of horned pitvipers from Vietnam and China are reviewed and the phylogenetic placement of four snakes from two sites in Tianjingshan Forest, China (Ruyan County, Guangdong Province; 24°43′N, 113°03′E, 563 m a.s.l.; 24°43′N, 113°02′E, 585 m a.s.l.) is examined. Using mitochondrial DNA sequence data (12S, 16S, ND4, cyt b; 2306 bp) and Bayesian and Maximum Likelihood analyses, the Tianjingshan pitvipers are revealed as sister to Protobothrops cornutus with a differentiation resembling those of P. flavoviridis and P. tokarensis. This indicates a close relationship with P. cornutus and suggests that Ceratrimeresurus shenlii Liang and Liu, 2003, previously considered a junior synonym of P. cornutus (Smith, 1930), could be a valid subspecies of P. cornutus or a recently split distinct species. However, further studies and samples from intermediate localities are needed to decide whether the observed differentiation reflects a pattern of isolation-by-distance or a phylogeographic, and thus perhaps taxonomically relevant, break.

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