Spectral preference of the predatory bugOrius sauteri, a biological control agent against agricultural pests

2016 ◽  
Author(s):  
Takumi Ogino
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Control Agent ◽  
Agricultural Pests

scholarly journals Generation of Trichoderma harzianum with pyr4 auxotrophic marker by using the CRISPR/Cas9 system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amanda A. Vieira ◽  
Giovanni R. Vianna ◽  
Jessica Carrijo ◽  
Francisco J. L. Aragão ◽  
Pabline M. Vieira
Keyword(s):  
Biological Control ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Phenotypic Characterization ◽  
Agricultural Pests ◽  
Control Activity ◽  
Guide Rna ◽  
Endogenous Expression ◽  
Auxotrophic Marker

AbstractTrichoderma harzianum is a filamentous fungus used as a biological control agent for agricultural pests. Genes of this microorganism have been studied, and their applications are patented for use in biofungicides and plant breeding strategies. Gene editing technologies would be of great importance for genetic characterization of this species, but have not yet been reported. This work describes mutants obtained with an auxotrophic marker in this species using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/ Cas (CRISPR-associated) system. For this, sequences for a guide RNA and Cas9 overexpression were inserted via biolistics, and the sequencing approach confirmed deletions and insertions at the pyr4 gene. Phenotypic characterization demonstrated a reduction in the growth of mutants in the absence of uridine, as well as resistance to 5-fluorotic acid. In addition, the gene disruption did not reduce mycoparasitc activity against phytopathogens. Thus, target disruption of the pyr4 gene in T. harzianum using the CRISPR/Cas9 system was demonstrated, and it was also shown that endogenous expression of the system did not interfere with the biological control activity of pathogens. This work is the first report of CRISPR Cas9-based editing in this biocontrol species, and the mutants expressing Cas9 have potential for the generation of useful technologies in agricultural biotechnology.

scholarly journals Paecilomyces and Its Importance in the Biological Control of Agricultural Pests and Diseases

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1746
Author(s):  
Alejandro Moreno-Gavíra ◽  
Victoria Huertas ◽  
Fernando Diánez ◽  
Brenda Sánchez-Montesinos ◽  
Mila Santos
Keyword(s):  
Biological Control ◽  
Crop Production ◽  
Biological Control Agent ◽  
Control Agent ◽  
Mechanisms Of Action ◽  
Biological Control Agents ◽  
Agricultural Pests ◽  
Pests And Diseases ◽  
Promising Strategy ◽  
Agricultural Use

Incorporating beneficial microorganisms in crop production is the most promising strategy for maintaining agricultural productivity and reducing the use of inorganic fertilizers, herbicides, and pesticides. Numerous microorganisms have been described in the literature as biological control agents for pests and diseases, although some have not yet been commercialised due to their lack of viability or efficacy in different crops. Paecilomyces is a cosmopolitan fungus that is mainly known for its nematophagous capacity, but it has also been reported as an insect parasite and biological control agent of several fungi and phytopathogenic bacteria through different mechanisms of action. In addition, species of this genus have recently been described as biostimulants of plant growth and crop yield. This review includes all the information on the genus Paecilomyces as a biological control agent for pests and diseases. Its growth rate and high spore production rate in numerous substrates ensures the production of viable, affordable, and efficient commercial formulations for agricultural use.

The potential of Mecysolobus erro (Curculionidae) as a biological control agent for Buddleia

2002 ◽  
Vol 55 ◽  
pp. 433-433
Author(s):  
B.A. Gresham ◽  
M.K. Kay ◽  
W. Faulds ◽  
T.M. Withers
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Control Agent

scholarly journals Limited host range in the idiobiont parasitoid Phymasticus coffea, a prospective biological control agent of the coffee pest Hypothenemus hampei in Hawaii

2021 ◽  
Author(s):  
Fazila Yousuf ◽  
Peter A. Follett ◽  
Conrad P. D. T. Gillett ◽  
David Honsberger ◽  
Lourdes Chamorro ◽  
...  
Keyword(s):  
Biological Control ◽  
Native Species ◽  
Biological Control Agent ◽  
Classical Biological Control ◽  
Environmental Safety ◽  
Control Agent ◽  
Hypothenemus Hampei ◽  
Parasitism Rate ◽  
Limited Host Range ◽  
Parasitoid Development

AbstractPhymastichus coffea LaSalle (Hymenoptera:Eulophidae) is an adult endoparasitoid of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera:Curculionidae:Scolytinae), which has been introduced in many coffee producing countries as a biological control agent. To determine the effectiveness of P. coffea against H. hampei and environmental safety for release in Hawaii, we investigated the host selection and parasitism response of adult females to 43 different species of Coleoptera, including 23 Scolytinae (six Hypothenemus species and 17 others), and four additional Curculionidae. Non-target testing included Hawaiian endemic, exotic and beneficial coleopteran species. Using a no-choice laboratory bioassay, we demonstrated that P. coffea was only able to parasitize the target host H. hampei and four other adventive species of Hypothenemus: H. obscurus, H. seriatus, H. birmanus and H. crudiae. Hypothenemus hampei had the highest parasitism rate and shortest parasitoid development time of the five parasitized Hypothenemus spp. Parasitism and parasitoid emergence decreased with decreasing phylogenetic relatedness of the Hypothenemus spp. to H. hampei, and the most distantly related species, H. eruditus, was not parasitized. These results suggest that the risk of harmful non-target impacts is low because there are no native species of Hypothenemus in Hawaii, and P. coffea could be safely introduced for classical biological control of H. hampei in Hawaii.

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