scholarly journals A Counting Chamber Peroxidase Reaction Method for Erythrocytes. A New Technique for Histo-chemical Examination of Peroxidase Activity of Erythrocytes

1953 ◽  
Vol 57 (4) ◽  
pp. 367-373
Author(s):  
Ototaka Higashi
Keyword(s):  
Peroxidase Activity ◽  
New Technique ◽  
Chemical Examination ◽  
Reaction Method ◽  
Peroxidase Reaction ◽  
Counting Chamber ◽  
A New Technique

scholarly journals A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine endproduct of peroxidase reaction.

1989 ◽  
Vol 37 (10) ◽  
pp. 1563-1565 ◽  
Author(s):  
I Merchenthaler ◽  
J Stankovics ◽  
F Gallyas
Keyword(s):  
Physical Development ◽  
New Technique ◽  
Step Method ◽  
Substrate Solution ◽  
Peroxidase Reaction ◽  
Additional Step ◽  
Highly Sensitive ◽  
One Step ◽  
A New Technique ◽  
Physical Developer

We have developed a new technique which makes silver intensification of the oxidatively polymerized diaminobenzidine (DAB), the endproduct of peroxidase reaction, less laborious without any loss in selectivity or sensitivity. The new technique is based on two strategies: (a) increasing the argyrophilia of the DAB by modifying its polymerization with Ni ions, and (b) decreasing tissue argyrophila by using a mildly acidic physical developer instead of the alkaline one previously presented. Because the nickel modification takes place in the DAB substrate solution, i.e., in the final step of the peroxidase reaction, only one additional step, the physical development, must be carried out if intensification is needed.

A New Technique for Counting Eggs ofFasciola hepaticain Cattle Faeces

1956 ◽  
Vol 30 (2-3) ◽  
pp. 165-172 ◽  
Author(s):  
W. Dorsman
Keyword(s):  
Fasciola Hepatica ◽  
New Technique ◽  
Measured Amount ◽  
Counting Chamber ◽  
Technical Details ◽  
Cattle Faeces ◽  
A New Technique

The essential features of the method, which is described in detail, are a sieving technique to collect quantitatively theFasciola hepaticaeggs of a sample of cattle faeces, a new technique for transferring quantitatively the eggs to a measured amount of water, and a new technique for obtaining even distribution of the eggs within a counting chamber. Technical details for manufacturing the apparatus and the counting slide, designed to perform the different stages of the method, are given. The correctness of the resulting counts is demonstrated.

A new technique for the mapping of oxygen tension on the brain surface

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S543-S543
Author(s):  
Satoshi Kimura ◽  
Keigo Matsumoto ◽  
Yoshio Imahori ◽  
Katsuyoshi Mineura ◽  
Toshiyuki Itoh
Keyword(s):  
Oxygen Tension ◽  
New Technique ◽  
Brain Surface ◽  
A New Technique ◽  
The Brain
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