scholarly journals Molecular Analysis of Base Damage Clustering Associated with a Site-Specific Radiation-Induced DNA Double-Strand Break

2006 ◽  
Vol 166 (5) ◽  
pp. 767-781 ◽  
Author(s):  
Kamal Datta ◽  
Pawel Jaruga ◽  
Miral Dizdaroglu ◽  
Ronald D. Neumann ◽  
Thomas A. Winters
Keyword(s):  
Molecular Analysis ◽  
Strand Break ◽  
Double Strand Break ◽  
Base Damage ◽  
Site Specific ◽  
Dna Double Strand Break ◽  
Radiation Induced ◽  
A Site

Enzymatic probing and structural mapping of a site-specific complex radiation-induced DNA double-strand break

2003 ◽  
Vol 57 (2) ◽  
pp. S346 ◽  
Author(s):  
K Datta ◽  
M Dizdaroglu ◽  
P Jaruga ◽  
R.D Neumann ◽  
T.A Winters
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Structural Mapping ◽  
Site Specific ◽  
Dna Double Strand Break ◽  
Specific Complex ◽  
Radiation Induced ◽  
A Site

scholarly journals Pathway utilization in response to a site-specific DNA double-strand break in fission yeast

2003 ◽  
Vol 22 (6) ◽  
pp. 1419-1430 ◽  
Author(s):  
J. Prudden
Keyword(s):  
Fission Yeast ◽  
Strand Break ◽  
Double Strand Break ◽  
Site Specific ◽  
Dna Double Strand Break ◽  
A Site

scholarly journals RAD-51-Dependent and -Independent Roles of a Caenorhabditis elegans BRCA2-Related Protein during DNA Double-Strand Break Repair

2005 ◽  
Vol 25 (8) ◽  
pp. 3127-3139 ◽  
Author(s):  
Julie S. Martin ◽  
Nicole Winkelmann ◽  
Mark I. R. Petalcorin ◽  
Michael J. McIlwraith ◽  
Simon J. Boulton
Keyword(s):  
Caenorhabditis Elegans ◽  
Strand Break ◽  
Double Strand Break ◽  
Nonhomologous End Joining ◽  
Related Protein ◽  
End Joining ◽  
Dsb Repair ◽  
Phenotypic Differences ◽  
Dna Double Strand Break ◽  
Radiation Induced

ABSTRACT The BRCA2 tumor suppressor is implicated in DNA double-strand break (DSB) repair by homologous recombination (HR), where it regulates the RAD51 recombinase. We describe a BRCA2-related protein of Caenorhabditis elegans (CeBRC-2) that interacts directly with RAD-51 via a single BRC motif and that binds preferentially to single-stranded DNA through an oligonucleotide-oligosaccharide binding fold. Cebrc-2 mutants fail to repair meiotic or radiation-induced DSBs by HR due to inefficient RAD-51 nuclear localization and a failure to target RAD-51 to sites of DSBs. Genetic and cytological comparisons of Cebrc-2 and rad-51 mutants revealed fundamental phenotypic differences that suggest a role for Cebrc-2 in promoting the use of an alternative repair pathway in the absence of rad-51 and independent of nonhomologous end joining (NHEJ). Unlike rad-51 mutants, Cebrc-2 mutants also accumulate RPA-1 at DSBs, and abnormal chromosome aggregates that arise during the meiotic prophase can be rescued by blocking the NHEJ pathway. CeBRC-2 also forms foci in response to DNA damage and can do so independently of rad-51. Thus, CeBRC-2 not only regulates RAD-51 during HR but can also function independently of rad-51 in DSB repair processes.

scholarly journals Homologous recombination pathway may play a major role in high-LET radiation-induced DNA double-strand break repair

2014 ◽  
Vol 55 (suppl 1) ◽  
pp. i83-i84 ◽  
Author(s):  
A. Gerelchuluun ◽  
J. Zhu ◽  
F. Su ◽  
A. Asaithamby ◽  
D. J. Chen ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Homologous Recombination Pathway ◽  
Dna Double Strand Break ◽  
High Let Radiation ◽  
High Let ◽  
Radiation Induced ◽  
Recombination Pathway

scholarly journals Yeast ATM and ATR use different mechanisms to spread histone H2A phosphorylation around a DNA double-strand break

2019 ◽  
Author(s):  
Kevin Li ◽  
Gabriel Bronk ◽  
Jane Kondev ◽  
James E. Haber
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Three Dimensions ◽  
One Dimension ◽  
Histone H2a ◽  
Directed Motion ◽  
Checkpoint Kinases ◽  
Dna Double Strand Break ◽  
Chromosome Iii ◽  
A Site

AbstractOne of the hallmarks of DNA damage is the rapid spreading of phosphorylated histone H2A (γ-H2AX) around a DNA double-strand break (DSB). In the budding yeast S. cerevisiae, nearly all H2A isoforms can be phosphorylated, either by Mec1ATR or Tel1ATM checkpoint kinases. We induced a site-specific DSB with HO endonuclease at the MAT locus on chromosome III and monitored the formation of γ-H2AX by ChIP-qPCR in order to uncover the mechanisms by which Mec1ATR and Tel1ATM propagate histone modifications across chromatin. With either kinase, γ-H2AX spreads as far as ∼50 kb on both sides of the lesion within 1 h; but the kinetics and distribution of modification around the DSB are significantly different. The total accumulation of phosphorylation is reduced by about half when either of the two H2A genes is mutated to the nonphosphorylatable S129A allele. Mec1 activity is limited by the abundance of its ATRIP partner, Ddc2. Moreover, Mec1 is more efficient than Tel1 at phosphorylating chromatin in trans – at distant undamaged sites that are brought into physical proximity to the DSB. We compared experimental data to mathematical models of spreading mechanisms to determine whether the kinases search for target nucleosomes by primarily moving in three dimensions through the nucleoplasm or in one dimension along the chromatin. Bayesian model selection indicates that Mec1 primarily uses a 3D diffusive mechanism, whereas Tel1 undergoes directed motion along the chromatin.

Ionizing radiation-induced growth in soft agar is associated with miR-21 upregulation in wild-type and DNA double strand break repair deficient cells

DNA Repair ◽  
2019 ◽  
Vol 78 ◽  
pp. 37-44 ◽  
Author(s):  
Siyuan Tang ◽  
Bailong Liu ◽  
Min Liu ◽  
Zhentian Li ◽  
Jiaqi Liu ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Strand Break ◽  
Soft Agar ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Wild Type ◽  
Dna Double Strand Break ◽  
Radiation Induced ◽  
Break Repair
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