CONCENTRATIONS OF IMMUNOREACTIVE THYROTROPHIC HORMONE IN URINE OF NORMAL SUBJECTS, PATIENTS WITH THYROID DISORDERS AND HYPOPITUITARISM, AND AFTER INFUSION OF HUMAN THYROTROPHIC HORMONE

1974 ◽  
Vol 62 (3) ◽  
pp. 645-655 ◽  
Author(s):  
S. F. KUKU ◽  
P. HARSOULIS ◽  
J. L. YOUNG ◽  
T. R. FRASER
Keyword(s):  
Urinary Excretion ◽  
Diurnal Variation ◽  
Normal Subjects ◽  
Thyroid Stimulating Hormone ◽  
Thyroid Disorders ◽  
Human Pituitary ◽  
Double Antibody ◽  
Thyrotrophic Hormone ◽  
Stimulating Hormone

SUMMARY The urinary excretion of thyroid-stimulating hormone (TSH) has been measured by double antibody radioimmunoassay after concentration by dialysis followed by lyophilization. Among 30 normal subjects, the excretion was 5·6 ± 0·31 (s.e.m.) μu./h. No diurnal variation nor differences between sexes were discerned. In 14 primary hypothyroid subjects the urinary excretion was raised (P < 0·001) to 25·1 ± 3·3 μu./h. In 14 hyperthyroid and 7 hypopituitary subjects subnormal levels of 2·6 ± 0·2 and 2·5 ± 0·22 μu./h (P < 0·001) respectively, were found. Serum and urinary TSH concentrations were measured before, during and after an infusion of human pituitary TSH (MRC 70/9) in two subjects and showed a correlation. Urinary TSH measurement is thus a good discriminant between normal and hyperthyroid or hypopituitary patients.

SEPHADEX GEL FILTRATION ANALYSIS OF IMMUNOREACTIVE THYROTROPHIC HORMONE IN HUMAN URINE

1974 ◽  
Vol 62 (3) ◽  
pp. 657-662
Author(s):  
S. F. KUKU ◽  
P. HARSOULIS ◽  
J. L. YOUNG ◽  
N. D. QUE ◽  
T. R. FRASER
Keyword(s):  
Human Urine ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Thyroid Stimulating Hormone ◽  
Single Peak ◽  
Human Pituitary ◽  
Thyrotrophic Hormone ◽  
Hypothyroid Patients ◽  
Serum Tsh ◽  
Single Symmetrical Peak

SUMMARY To assess whether urinary immunoassayable thyroid-stimulating hormone (TSH) differed from pituitary and serum TSH, urinary concentrates from two hypothyroid subjects were analysed by Sephadex G-100 gel filtration. The elution profiles, measured by radioimmunoassay, were then compared with those of neat sera from hypothyroid patients and human pituitary TSH preparations. The pituitary preparations and the hypothyroid serum were eluted as a comparable single symmetrical peak corresponding to that obtained from a highly purified radio-iodinated human TSH of pituitary origin; no evidence of 'big' TSH emerged. In contrast, however, the material eluted from the hypothyroid urine concentrates not only revealed an asymmetrical peak corresponding to that described above but several other minor peaks eluting later and probably corresponding to fragments of TSH. When human pituitary TSH was infused into two normal subjects, gel filtration analysis of concentrates from urinary samples obtained during and at fixed periods after the infusion revealed a single peak during the infusion but more peaks appeared with the later samples.

Elevations in Thyroid-Stimulating Hormone in Normal Subjects after Receiving Short-Term Lithium Carbonate

1988 ◽  
Vol 22 (3) ◽  
pp. 202-204 ◽  
Author(s):  
Merlin V. Nelson ◽  
Vickie Tutag-Lehr ◽  
R. Lee Evans
Keyword(s):  
Lithium Carbonate ◽  
Normal Subjects ◽  
Thyroid Stimulating Hormone ◽  
Short Term ◽  
The Mean ◽  
Early Rise ◽  
State Concentration ◽  
Male Subjects ◽  
Stimulating Hormone

Nine normal, healthy male subjects had significantly elevated thyroid-stimulating hormone (TSH) concentrations while receiving oral lithium carbonate for two weeks. The mean minimum lithium serum concentration was 0.765 mEq/L. The TSH concentrations after 15 days on lithium were significantly correlated to the TSH concentration at baseline. No correlation was found between mean minimum lithium steady-state concentration and TSH concentration after 15 days on lithium. Further research is necessary to determine if a high baseline TSH concentration or an early rise in TSH will predict those patients who will eventually develop hypothyroidism after long-term lithium therapy.

THYROID-STIMULATING HORMONE AND TRIIODOTHYRONINE AS AIDS IN THE DIAGNOSIS OF THYROID DISORDERS

1960 ◽  
Vol 9 (3) ◽  
pp. 194-199 ◽  
Author(s):  
BRYAN HUDSON ◽  
DORA WINIKOFF ◽  
H. PINCUS TAFT ◽  
F. I. R. MARTIN
Keyword(s):  
Thyroid Stimulating Hormone ◽  
Thyroid Disorders ◽  
Stimulating Hormone

DEXTRAN-COATED CHARCOAL USED IN THE RADIOIMMUNOASSAY OF HUMAN PITUITARY LUTEINIZING HORMONE

1973 ◽  
Vol 73 (3) ◽  
pp. 444-454 ◽  
Author(s):  
T. Sand ◽  
P. A. Torjesen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Protein Composition ◽  
Thyroid Stimulating Hormone ◽  
Human Chorionic Gonadotrophin ◽  
Human Thyroid ◽  
Serum Samples ◽  
Human Pituitary ◽  
The Difference ◽  
Stimulating Hormone

ABSTRACT A radioimmunoassay for human pituitary luteinizing hormone (LH) using charcoal for the separation of free from antibody-bound hormone is described. The ability of the various types of charcoal preparations tested to separate free from antibody-bound hormone differed greatly as did the amount required to give maximum adsorption of free hormone. It was also found that the adsorption of free and antibody-bound hormone was greatly influenced by the presence of other proteins. Hence it was necessary to add human serum to the standard tubes before the addition of the charcoal-dextran suspension, in order to compensate for the difference in protein composition between the standards and the serum samples. Two antisera obtained from rabbits immunized with human chorionic gonadotrophin (HCG) were used. One of the antisera had an affinity to human thyroid-stimulating hormone (TSH) and human follicle-stimulating hormone (FSH) of less than 10% as compared to that of LH, while the other had an affinity of about 30 % as compared to that of LH.

scholarly journals Lithium and the thyroid

1996 ◽  
Vol 20 (6) ◽  
pp. 354-355 ◽  
Author(s):  
Carol Paton ◽  
Dominic Beer
Keyword(s):  
Thyroid Disease ◽  
Adult Population ◽  
Thyroid Stimulating Hormone ◽  
Lifetime Risk ◽  
Thyroid Autoantibodies ◽  
Thyroid Disorders ◽  
The Common ◽  
Stimulating Hormone

The common thyroid disorders are all autoimmune in origin, with the lifetime risk of thyroid disease being 1–2%. Thyroid autoantibodies are present in 9% of the adult population and 12.7% of women, with the frequency rising steeply in women over 45 years of age (Myers & West, 1987). In addition, 20% of the over 60–year-olds have an abnormal (raised) thyroid stimulating hormone (TSH). The presence of both raised TSH and thyroid autoantibodies is associated with the development of clinical hypothyroidism at the rate of 5% per year (Myers & West, 1987).

Notes on the Radioimmunoassay of Human Thyrotrophin

1975 ◽  
Vol 12 (1-6) ◽  
pp. 196-199 ◽  
Author(s):  
P. J. Wood ◽  
Janet Smith ◽  
V. Marks
Keyword(s):  
Thyroid Stimulating Hormone ◽  
Human Thyroid ◽  
Free Protein ◽  
Standard Curve ◽  
Double Antibody ◽  
Acceptable Standard ◽  
Tsh Levels ◽  
Stimulating Hormone

This paper describes the use of screened transfusion plasma as a source of human thyroid stimulating hormone (h-TSH) free protein for standardization of a conventional “double antibody” h-TSH radioimmunoassay. Serum and plasma immunoreactive h-TSH levels have been shown to be stable for up to four days at 4°C and 25°C. A 37°C incubation procedure has been developed which gives an acceptable standard curve, but with some loss of “between batch” precision.

THE DIURNAL VARIATION OF PLASMA LEVELS AND URINARY EXCRETION OF 17-HYDROXYCORTICOSTEROIDS IN NORMAL SUBJECTS, NIGHT WORKERS AND BLIND SUBJECTS*†

1956 ◽  
Vol 16 (5) ◽  
pp. 622-633 ◽  
Author(s):  
CLAUDE J. MIGEON ◽  
FRANK H. TYLER ◽  
JOHN P. MAHONEY ◽  
ANGEL A. FLORENTIN ◽  
HILMON CASTLE ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Diurnal Variation ◽  
Plasma Levels ◽  
Normal Subjects
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