Prolactin stimulates steroidogenesis by human luteal tissue in vitro

1984 ◽  
Vol 103 (1) ◽  
pp. 107-110 ◽  
Author(s):  
M. G. Hunter
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Luteal Tissue ◽  
Oestradiol Production ◽  
Human Corpus Luteum

ABSTRACT Human luteal tissue recovered from varying stages of the luteal phase was minced and incubated for 3 h and the effect of human chorionic gonadotrophin (hCG), prolactin and hCG + prolactin on progesterone and oestradiol production measured. While hCG generally enhanced both progesterone and oestradiol synthesis, prolactin alone at either 20 or 200 μg/l had no significant effect on steroidogenesis. When prolactin was added along with hCG in four of six corpora lutea, however, progesterone production significantly increased and in three of six corpora lutea oestradiol production was increased above that induced by hCG alone. It is concluded that prolactin may play some role in the control of steroidogenesis by the human corpus luteum. J. Endocr. (1984) 103, 107–110

STIMULATION BY HUMAN CHORIONIC GONADOTROPHIN OF OESTRADIOL PRODUCTION BY DISPERSED CELLS FROM HUMAN CORPUS LUTEUM: COMPARISON WITH PROGESTERONE PRODUCTION; UTILIZATION OF EXOGENOUS TESTOSTERONE

1981 ◽  
Vol 91 (2) ◽  
pp. 197-203 ◽  
Author(s):  
M. C. RICHARDSON ◽  
G. M. MASSON
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cell Suspensions ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Late Luteal Phase ◽  
Oestradiol Production ◽  
Dispersed Cells

Cell suspensions were prepared from tissue samples of human corpora lutea obtained during the mid- and late-luteal phase of the menstrual cycle. Both oestradiol and progesterone production by dispersed cells were stimulated by similar concentrations of human chorionic gonadotrophin (hCG). As the degree of stimulation of production by hCG was greater for progesterone than for oestradiol (five- to tenfold compared with two- to threefold higher than basal production), the ratio of progesterone to oestradiol produced varied according to the level of trophic stimulation. A comparison of cell suspensions prepared from mid- and late-luteal phase corpora lutea, exposed to the same concentration of hCG (10 i.u./ml) in vitro, did not reveal a shift to oestradiol production in the late-luteal phase. Provision of additional testosterone during incubation raised the level of oestradiol production by dispersed luteal cells. At an optimum concentration of testosterone (1 μmol/l), oestradiol synthesis was not raised further in the presence of hCG or N6, O2-dibutyryl cyclic AMP, suggesting a lack of induction or activation of the aromatase system by gonadotrophin in short-term cultures. Basal and stimulated levels of progesterone production were not significantly impaired in the presence of testosterone.

Oxytocin may play a role in the control of the human corpus luteum

1982 ◽  
Vol 95 (1) ◽  
pp. 65-70 ◽  
Author(s):  
G. J. S. Tan ◽  
R. Tweedale ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Inhibitory Action ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Human Corpus Luteum

The effects of oxytocin on dispersed luteal cells from human corpora lutea of the menstrual cycle were studied. Oxytocin at a concentration of 4 mi.u./ml produced a slight increase in basal progesterone production. However, higher oxytocin concentrations (400 and 800 mi.u./ml) markedly inhibited both basal and human chorionic gonadotrophin-induced progesterone production. These data provide evidence for an effect of oxytocin on the human corpus luteum. In view of the inhibitory action of oxytocin, increased secretion of this hormone may be important in the demise of the corpus luteum at the end of the menstrual cycle.

Control of inhibin production by dispersed human luteal cells in vitro

1992 ◽  
Vol 4 (1) ◽  
pp. 67 ◽  
Author(s):  
HZ Wang ◽  
SH Lu ◽  
XJ Han ◽  
ZD Sun ◽  
WX Shen ◽  
...  
Keyword(s):  
Sex Steroids ◽  
Follicle Stimulating Hormone ◽  
Calf Serum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Oestradiol Production ◽  
Human Corpus Luteum

The production of inhibin in vitro by dispersed cells from early to mid (Days 16-19) and late stage (Day 23) human corpus luteum (CL) was examined, and the effects of human chorionic gonadotrophin (hCG), follicle stimulating hormone (FSH), oestradiol and testosterone on inhibin production were determined. Corpora lutea from five subjects in the early to mid luteal stage and three subjects in late luteal stage were dispersed with enzymes and the luteal cells cultured in medium supplemented with 5% calf serum and either FSH (1, 10 or 100 ng mL-1), oestradiol-17 beta (2.5, 5 or 10 micrograms mL-1) or testosterone (0.25, 1 or 5 micrograms mL-1) with or without hCG (1 I.U. mL-1). Cells were cultured for 1 to 3 days without changes of medium, and the concentrations of progesterone, oestradiol and immunoreactive inhibin in the medium were measured by radioimmunoassay. Cells from both types of CL produced inhibin in vitro under basal conditions, but only cells from early to mid CLs responded to hCG with a significant increase in inhibin production. Both progesterone and oestradiol production were stimulated by hCG in both groups of CL. Inhibin concentrations in the cell cultures declined with time in culture, particularly in the late CL group, whereas the concentration of steroids increased. Neither FSH, oestradiol nor testosterone significantly changed inhibin production in either CL group. It was concluded that inhibin production by human luteal cells in vitro is influenced by the age of the CL, and is dependent on LH (hCG) but not on FSH or sex steroids.

Effects of prolactin on steroid production by human luteal cells in vitro

1983 ◽  
Vol 96 (3) ◽  
pp. 499-503 ◽  
Author(s):  
G. J. S. Tan ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Ovarian Function ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Short Term ◽  
Steroid Production ◽  
Luteal Cells ◽  
Progesterone Production

The effects of prolactin on steroidogenesis were studied in dispersed luteal cells prepared from human corpora lutea of the menstrual cycle. Prolactin, at concentrations of 0·1–1000 ng/ml, had no effect on progesterone production by luteal cells during short-term incubation (3 h). However, in two out of five corpora lutea, higher concentrations of prolactin (100 and 1000 ng/ml) significantly reduced the oestradiol-17β production induced by human chorionic gonadotrophin (hCG; 10 i.u./ml); lower doses of prolactin had little effect. In the remaining corpora lutea, prolactin failed to affect either basal or hCG-induced production of oestradiol-17β. These results are discussed in relation to the mechanism by which prolactin influences human ovarian function.

103 Effects of administration of human chorionic gonadotrophin at Day 5 post-ovulation on development of the original corpus luteum depend on the locational relationship between the original and accessory corpora lutea

2020 ◽  
Vol 32 (2) ◽  
pp. 177
Author(s):  
K. Hazano ◽  
S. Haneda ◽  
M. Matsui
Keyword(s):  
Corpus Luteum ◽  
Repeated Measures ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Intrauterine Environment ◽  
Prostaglandin F2a ◽  
Tissue Area ◽  
Luteal Tissue ◽  
Relationship Of

In cattle, human chorionic gonadotrophin (hCG) is administered at Day 5 post-ovulation to improve fertility. This treatment can induce ovulation of the first-wave dominant follicle (W1DF), from which an accessory corpus luteum (CL) is generated. In addition, hCG has the effect of promoting CL development. It is possible that the locational relationship between the original and accessary CLs influences the effect of hCG on CL development, because the locational relationship of the CLs affects intraovarian blood flow. The present study aimed to clarify whether the locational relationship between the original and accessory CLs influences the effect of hCG on their development. Cross-bred beef heifers (Holstein×Japanese Black, n=56) were used for the present study. The oestrus cycle was synchronized using oestradiol benzoate (EB) and a controlled internal drug release (CIDR)-based program. Briefly, an administration of EB (2mg) with 9-day CIDR insertion was followed by administration of prostaglandin F2a analogue (PGF2a) on the day of CIDR removal, EB (1mg) 1 day after a PGF2a injection, and GnRH 12h after the second EB injection. At Day 5 post-ovulation, the locational relationship between the original CL and the W1DF was confirmed using transrectal ultrasonography (USG), and two groups were defined: ipsilateral group (IG; n=30), in which the CL and the W1DF are in the same ovary, and contralateral group (CG; n=26), in which the CL and the W1DF are in separate ovaries. Moreover, IG and CG were respectively subdivided into two groups, with or without hCG (1500IU) treatment (IG/hCG, n=15; IG without hCG, n=15, and CG/hCG, n=14; CG without hCG, n=12). The diameter and luteal tissue area (i.e. minus the cavity area) of the original CL and the accessory CL were examined at Days 5, 7, and 14, using USG. Two-way repeated-measures ANOVA was used to compare the diameter and luteal tissue area between IG/hCG and IG without hCG, and between CG/hCG and CG without hCG. In CG, the diameter (P<0.01) and luteal tissue area of the original CL (P<0.001) at Day 7 was increased by receiving hCG, while it did not change in IG. The diameter and luteal tissue area of the original CL at Day 14 were not affected by the administration of hCG in either CG or IG. Moreover, for the accessory CL, no difference of the diameter and luteal tissue area was observed between CG and IG. The present study showed that hCG treatment at Day 5 post-ovulation stimulate the growth of the original CL at Day 7, when the original CL and accessory CL are on contralateral sides. Our results suggest that the effect of administration of the hCG at Day 5 post-ovulation on the original CL development depends on the locational relationship between the original and accessory CL (IG or CG). The function of the CL affects the intrauterine environment for embryonic development. Therefore, it is necessary to investigate the effect of the hCG injection at Day 5 on the function of CL (i.e. plasma P4 concentration) in IG and CG, respectively.

FORMATION OF STEROID HORMONES IN VITRO BY DEVELOPING CORPORA LUTEA OF THE RABBIT

1977 ◽  
Vol 75 (3) ◽  
pp. 355-361 ◽  
Author(s):  
AKIRA SUZUKI ◽  
TAKAHIDE MORI ◽  
TOSHIO NISHIMURA
Keyword(s):  
Corpus Luteum ◽  
Initial Step ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Temporary Increase ◽  
Corpora Lutea ◽  
Sharp Rise ◽  
17 Hydroxyprogesterone ◽  
Qualitative Changes

Rabbits were injected with human chorionic gonadotrophin (HCG), and slices of developing corpora lutea taken from the ovaries 15, 18, 24, 48, 72 and 96 h after injection were incubated with [1-14C]sodium acetate at 37 °C for 3 h. The incorporation of labelled acetate into ten steroids, including progestagens, androgens and oestrogens, was analysed. In the initial step of corpus luteal formation, the specific incorporation (incorporation of [1-14C]acetate/100 mg tissue) increased sharply. The major steroidal products were progesterone, 17-hydroxyprogesterone and 20α-hydroxypregn-4-en-3-one. Between 18 and 48 h, the increase in specific incorporation was more gradual than in the initial step. Although the pattern was also dominated by progestagens, a temporary increase in the incorporation of acetate into androgens and oestrogens was observed. In the final step, a sharp rise in the total incorporation (incorporation of [1-14C]acetate/corpus luteum) was found, whereas the specific incorporation increased only slightly. The principal steroids produced were progesterone, pregnenolone and 20α-hydroxypregn-4-en-3-one. Incorporation into C19 steroids declined markedly and that into C18 steroids could not be detected. This profile of steroidogenesis 96 h after injection of HCG was similar to that of the corpus luteum in pregnancy. Thus marked quantitative and qualitative changes have been demonstrated during the period of formation of corpora lutea in the rabbit.

Lack of direct inhibitory action of oxytocin on progesterone production by dispersed cells from human corpus luteum

1985 ◽  
Vol 104 (1) ◽  
pp. 149-151 ◽  
Author(s):  
M. C. Richardson ◽  
G. M. Masson
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
Inhibitory Action ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Menstrual Cycles ◽  
Progesterone Production ◽  
Human Corpus Luteum ◽  
Dispersed Cells

ABSTRACT Suspensions of luteal cells were prepared from samples of human corpora lutea obtained during the luteal phase of menstrual cycles. Addition of oxytocin (1 μmol/l) to the various cell preparations had no effect on either basal production of progesterone or on steroidogenic responses to a range of concentrations of gonadotrophin. J. Endocr. (1985) 104, 149–151

Effects of human chorionic gonadotrophin administration on Day 5 after oestrus on corpus luteum characteristics, circulating progesterone and conceptus elongation in cattle

2012 ◽  
Vol 24 (3) ◽  
pp. 472 ◽  
Author(s):  
D. Rizos ◽  
S. Scully ◽  
A. K. Kelly ◽  
A. D. Ealy ◽  
R. Moros ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Intramuscular Injection ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Control Group ◽  
Strong Positive Correlation ◽  
Serum Concentrations ◽  
Luteal Tissue ◽  
Highly Correlated

The aim of the present study was to test the hypothesis that elevated concentrations of progesterone (P4) resulting from the induction of an accessory corpus luteum (CL) by human chorionic gonadotrophin (hCG) administration on Day 5 after oestrus would lead to advanced conceptus elongation on Day 14 following embryo transfer on Day 7. The oestrous cycles of cross-bred beef heifers were synchronised and animals were randomly assigned to receive either of two treatments: (1) intramuscular injection of 3000 IU hCG on Day 5 after oestrus (n = 14); or (2) intramuscular injection of saline on Day 5 after oestrus (n = 13). Ovaries were scanned daily by transrectal ultrasonography to assess CL development. Serum concentrations of P4 were determined from daily blood samples collected from the jugular vein. In vitro-produced bovine blastocysts were transferred to synchronised recipients on Day 7 after oestrus (n = 15 blastocysts per recipient). Heifers were killed on Day 14 after oestrus and the uterus was flushed to recover the embryos. Injection of hCG on Day 5 induced ovulation of the dominant follicle in all treated heifers and increased the total area of luteal tissue on the ovary, which was associated with a significant increase (P < 0.001) in serum concentrations of P4 from Day 7 to Day 14. Positive associations were detected between circulating P4 with CL area (within-day correlations ranging from r = 0.45 to r = 0.67) and total area of luteal tissue (within-day correlations ranging from r = 0.65 to r = 0.86) Administration of hCG did not affect the proportion of Day 14 conceptuses recovered. However, compared with the control group, hCG-treated heifers had increased conceptus length (3.91 ± 1.23 vs 5.57 ± 1.02 mm, respectively; P = 0.06), width (1.00 ± 0.06 vs 1.45 ± 0.05 mm, respectively; P = 0.002) and area (5.71 ± 0.97 vs 8.31 ± 0.83, respectively; P = 0.02). Although numerically greater, mean interferon-τ (IFNT) production in vitro did not differ significantly (P = 0.54) between embryos recovered from hCG-treated and control heifers. In contrast, there was a strong positive correlation between individual embryo length (r = 0.76; P < 0.001) and individual embryo area (r = 0.72; P < 0.001) and IFNT production. In conclusion, administration of hCG on Day 5 after oestrus resulted in the formation of an accessory CL and hypertrophy of the original CL, the result of which was an increase in P4 concentrations from Day 7 onwards. These elevated P4 concentrations were associated with an increased conceptus area. Furthermore, conceptus size was highly correlated with IFNT secretion in vitro.

Inhibitory action of chemically deglycosylated human chorionic gonadotrophin on hormone-induced steroid production by dispersed cells from human corpus luteum

1984 ◽  
Vol 101 (3) ◽  
pp. 327-332 ◽  
Author(s):  
M. C. Richardson ◽  
G. M. Masson ◽  
M. R. Sairam
Keyword(s):  
Corpus Luteum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Maximum Response ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Anhydrous Hydrogen Fluoride ◽  
Tenfold Excess ◽  
Human Corpus Luteum ◽  
Dispersed Cells

ABSTRACT The biological activity of deglycosylated human chorionic gonadotrophin (hCG) prepared by treatment of the native hormone with anhydrous hydrogen fluoride was evaluated using suspensions of dispersed cells from biopsies of human corpus luteum obtained during the luteal phase of normal menstrual cycles. A reproducible pattern of response to hCG in terms of progesterone production by luteal cells was established for a range of luteal ages. Deglycosylation of hCG led to a diminished level of maximum response to the hormone. Co-incubation of luteal cells with a level of hCG just sufficient to elicit a maximum response and increasing concentrations of deglycosylated hCG led to a progressive inhibition of the hormonal response; at a concentration of 103 ng deglycosylated hCG/ml (a tenfold excess of deglycosylated hCG over the native hormone), hCG-induced progesterone production was reduced by about 50%. Deglycosylated hCG therefore acts as a partial antagonist for the action of hCG on human luteal cells. J. Endocr. (1984) 101, 327–332

Heterogeneity in the luteal population following superovulation with pregnant mare serum gonadotrophin and human chorionic gonadotrophin in the sheep

1989 ◽  
Vol 121 (3) ◽  
pp. 459-465 ◽  
Author(s):  
M. G. Hunter ◽  
J. A. Southee
Keyword(s):  
Luteal Phase ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Plasma Progesterone ◽  
Progesterone Synthesis ◽  
Luteal Tissue ◽  
The Individual ◽  
Effect Of Age ◽  
Pregnant Mare Serum Gonadotrophin

ABSTRACT In order to investigate the development and possible heterogeneity in the luteal population following superovulation, anoestrous ewes were induced to ovulate using progestagen priming followed by injections of pregnant mare serum gonadotrophin (1000 IU) and hCG (1000 IU). Ovaries were recovered from ewes on each of days 2, 4, 6, 8, 10, 12 and 15, and the weight, progesterone content, 125I-labelled hCG binding and progesterone synthesis in vitro of the individual corpora lutea measured. The results obtained showed that plasma progesterone concentrations on the day of slaughter were significantly correlated with time (P<0·05), total weight of luteal tissue (P< 0·001) and number of corpora lutea (P<0·05). The number of corpora lutea recovered per animal ranged from two to 12 and was significantly (P<0·05) correlated with the day after hCG injection until day 10. There was much variation between individual corpora lutea, particularly in terms of weight and progesterone content, although both were significantly (P<0·001) correlated with day of recovery until day 10. 125I-Labelled hCG binding was significantly (P<0·001) correlated with time until day 15. There was a significant (P<0·001) effect of age of the tissue on progesterone production in vitro, with output declining throughout the luteal phase. These results show that the number of corpora lutea induced by superovulation in anoestrous ewes was very variable, and suggest that ovulation may have continued to occur during the luteal phase. Moreover, there was much variation between individual corpora lutea recovered from the same animal, and thus it should not be assumed that an individual corpus luteum is representative of the entire population. Journal of Endocrinology (1989) 121, 459–465

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