Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line
Keyword(s):
Rt Pcr
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GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.
2014 ◽
Vol 41
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pp. 935-946
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2018 ◽
Vol 46
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pp. 364-372
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1991 ◽
Vol 179
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pp. 572-578
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pp. 723-733
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1988 ◽
Vol 85
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pp. 6528-6532
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2018 ◽
Vol 105
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pp. 184-196
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