Oxidative stress biomarkers in gills of brown trout (Salmo trutta m. fario) vaccinated against furunculosis

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

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Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

Scientific Reports ◽

10.1038/s41598-021-85127-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Natalia Kurhaluk ◽

Halyna Tkachenko

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Muscle Tissue ◽

Salmo Trutta ◽

Protein Carbonyl ◽

Oxidative Modification ◽

Oxidative Stress Biomarkers ◽

Development Stages ◽

Stress Biomarkers ◽

Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

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Induction and activity of oxidative stress-related proteins during waterborne Cu-exposure in brown trout (Salmo trutta)

Chemosphere ◽

10.1016/j.chemosphere.2006.04.088 ◽

2006 ◽

Vol 65 (10) ◽

pp. 1707-1714 ◽

Cited By ~ 39

Author(s):

Bjørn Henrik Hansen ◽

Svein Rømma ◽

Liv I.R. Søfteland ◽

Pål A. Olsvik ◽

Rolf A. Andersen

Keyword(s):

Oxidative Stress ◽

Brown Trout ◽

Salmo Trutta ◽

Brown Trout Salmo Trutta ◽

Related Proteins

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Induction and activity of oxidative stress-related proteins during waterborne Cd/Zn-exposure in brown trout (Salmo trutta)

Chemosphere ◽

10.1016/j.chemosphere.2006.12.048 ◽

2007 ◽

Vol 67 (11) ◽

pp. 2241-2249 ◽

Cited By ~ 72

Author(s):

Bjørn Henrik Hansen ◽

Svein Rømma ◽

Øyvind Aaberg Garmo ◽

Sindre Andre Pedersen ◽

Pål Asgeir Olsvik ◽

...

Keyword(s):

Oxidative Stress ◽

Brown Trout ◽

Salmo Trutta ◽

Brown Trout Salmo Trutta ◽

Related Proteins

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Do polystyrene microplastics affect juvenile brown trout (Salmo trutta f. fario) and modulate effects of the pesticide methiocarb?

10.21203/rs.2.19551/v1 ◽

2019 ◽

Author(s):

Hannah Schmieg ◽

Sven Huppertsberg ◽

Thomas P. Knepper ◽

Stefanie Krais ◽

Katharina Reitter ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Brown Trout ◽

Salmo Trutta ◽

Stress Protein ◽

The Public ◽

Protein Levels ◽

Tissue Integrity ◽

Brown Trout Salmo Trutta ◽

Juvenile Brown Trout

Abstract Background During the last decade, there has been rising interest of the scientific community and the public in the environmental risk related to the abundance of microplastics in aquatic environments. Besides potential effects of the particles themselves, also their interaction with organic micropollutants is of particular concern. Up to now, however, scientific knowledge in this context is scarce and insufficient for a reliable risk assessment. This is especially true for data on microplastics in freshwater ecosystems.Results Against the background of this shortage, we investigated possible adverse effects of polystyrene particles (10 4 particles/L) and the pesticide methiocarb (1 mg/L) both alone as well as in combination in juvenile brown trout ( Salmo trutta f. fario ) after a 96 h laboratory exposure. PS beads (density 1.05 g/mL) were cryogenically milled and fractionated resulting in irregular shaped particles (<50 µm). Besides body weight of the animals, biomarkers for proteotoxicity (stress protein family Hsp70), oxidative stress (superoxide dismutase, lipid peroxidation) and neurotoxicity (acetylcholinesterase, carboxylesterases) were analysed. As an indicator of overall health histopathological effects were studied in liver and gills of exposed fish. Polystyrene particles alone did not influence any of the investigated biomarkers. In contrast, the exposure to methiocarb led to a significant reduction of the activity of acetylcholinesterase and the two carboxylesterases. Moreover, the tissue integrity of liver and gills was impaired by the pesticide. Body weight, the oxidative stress and the stress protein levels were not influenced by methiocarb. Effects caused by the mixture of polystyrene microplastics and methiocarb were the same as those caused by methiocarb alone.Conclusions Overall, methiocarb led to strong effects in juvenile brown trout. In contrast, polystyrene microplastics in the tested concentration did not negatively affect the health of juvenile brown trout and did not modulate the toxicity of methiocarb in this fish species.

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Oxidative stress and partial migration in brown trout (Salmo trutta)

Canadian Journal of Zoology ◽

10.1139/cjz-2016-0312 ◽

2017 ◽

Vol 95 (11) ◽

pp. 829-835 ◽

Cited By ~ 6

Author(s):

Kim Birnie-Gauvin ◽

Kathryn S. Peiman ◽

Martin H. Larsen ◽

Henrik Baktoft ◽

Kim Aarestrup ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Brown Trout ◽

Salmo Trutta ◽

Oxidative Status ◽

Partial Migration ◽

Migration Strategy ◽

Brown Trout Salmo Trutta ◽

And Migration

During migration, animals are typically limited by their endogenous energetic resources that must be allocated to the physiological costs associated with locomotion, as well as avoiding and (or) compensating for oxidative stress. To date, there have been few attempts to understand the role of oxidative status in migration biology, particularly in fish. Semi-anadromous brown trout (Salmo trutta L., 1758) exhibit partial migration, where some individuals smoltify and migrate to sea, and others become stream residents, providing us with an excellent model to investigate the link between oxidative stress and migration. Using the brown trout, we obtained blood samples from juveniles from a coastal stream in Denmark in the fall prior to peak seaward migration that occurs in the spring, and assayed for antioxidant capacity (oxygen radical absorbance capacity) and oxidative stress levels (ratio of oxidized to reduced glutathione). We found that individuals that migrated had higher antioxidant capacity than residents and that future migration date was negatively correlated with both antioxidant capacity and body length in the fall. This study provides the first evidence that oxidative status is associated with migration strategy and timing, months in advance of the actual migration, and provides insight into the role of oxidative status in animal migration.

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Oxidative Stress, Lysosome Functioning, and Element Interactions in the Muscles of the Sea Trout Salmo Trutta m. Trutta L. Depending on Its Life Cycle

10.21203/rs.3.rs-116466/v1 ◽

2020 ◽

Author(s):

Natalia Kurhaluk ◽

Halyna Takchenko

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Muscle Tissue ◽

Salmo Trutta ◽

Protein Carbonyl ◽

Oxidative Modification ◽

Oxidative Stress Biomarkers ◽

Development Stages ◽

Stress Biomarkers ◽

Element Contents

Abstract The aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS>OMP KD>OMP AD>TAC, AcP>NAG>LAP>AAP and Cu>Fe>Ca>Mn>Zn>Mg, respectively.

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