LC-MS/MS Method for the Quantitative Determination of Tanespimycin and its Active Metabolite in Human Plasma: Method Validation and Overcoming an Insidious APCI Source Phenomenon

Introduction. Viral infections are a serious problem that occurs during the use of immunosuppressants in preparation for organ transplantation and in the postoperative period. Cytomegalovirus (CMV) infection is one of the main causes of diseases in people with weakened immune systems. It has a direct impact on one’s body and makes it more likely to reject a transplanted organ. Antiviral drugs are used to treat and prevent this infectious disease. Valganciclovir is a prodrug whose active metabolite is ganciclovir. Valganciclovir is the drug of choice in the treatment of CMV infections. Currently, there are no researches on the matter of simultaneous determination of both valganciclovir and ganciclovir in human blood plasma by means of high-performance liquid chromatography (HPLC) with ultraviolet detection. This research delivers a thorough description of development and validation of a particular method for simultaneous determination of valganciclovir and ganciclovir in the plasma after sample preparation by the method of protein precipitation.Aim. The aim of this study is to develop method for the quantitative determination of valganciclovir and its active metabolite ganciclovir in human plasma by HPLC-UV for pharmacokinetic studies.Materials and methods. Quantitative determination of tadalafil in plasma by HPLC-UV. A sample was prepared using protein precipitation.Results and discussion. This method was validated by next validation parameters: selectivity, matrix effect, calibration curve, accuracy, precision, lower limit of quantification, carry-over and stability.Conclusion. The method of the quantitative determination of valganciclovir and its active metabolite ganciclovir in human plasma was developed and validated by HPLC-UV. The analytical range of the was 5,0–1000,0 ng/ml for valganciclovir and 100,0–10000,0 ng/ml for ganciclovir in plasma. Method could be applied to determination of valganciclovir and ganciclovir in plasma for PK and BE studies.

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An HPLC-MS/MS Method for the Quantitative Determination of Ticagrelor and its Active Metabolite AR-C124910XX in Human Plasma and its Application to a Clinical Study

Current Pharmaceutical Analysis ◽

10.2174/1573412912666160518151458 ◽

2017 ◽

Vol 13 (3) ◽

pp. 296-303 ◽

Cited By ~ 4

Author(s):

Jingbo Peng ◽

Yicheng Wang ◽

Mupeng Li ◽

Chenjie He ◽

Yao Chen ◽

...

Keyword(s):

Clinical Study ◽

Quantitative Determination ◽

Human Plasma ◽

Active Metabolite

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Quantitative determination of buprenorphine and its active metabolite, norbuprenorphine, in human plasma by gas chromatography—chemical ionization mass spectrometry

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/s0378-4347(00)83057-1 ◽

1989 ◽

Vol 487 ◽

pp. 469-475 ◽

Cited By ~ 19

Author(s):

Michiteru Ohtani ◽

Fuminori Shibuya ◽

Hajime Kotaki ◽

Katsuyoshi Uchino ◽

Yukiya Saitoh ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Quantitative Determination ◽

Human Plasma ◽

Active Metabolite ◽

Chemical Ionization ◽

Ionization Mass Spectrometry ◽

Chemical Ionization Mass Spectrometry ◽

Ionization Mass

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Determination of Lappaconitin, Diterpene Alkaloide Obtained from Plants Aconitum leucostomum, and its Active Metabolite N-desacetyllappaconitin in Human Plasma and Blood

Drug development & registration ◽

10.33380/2305-2066-2021-10-3-105-113 ◽

2021 ◽

Vol 10 (3) ◽

pp. 105-113

Author(s):

O. A. Archakova ◽

T. N. Komarov ◽

A. V. Rogov ◽

D. S. Shchelgacheva ◽

A. A. Aleshina ◽

...

Keyword(s):

Quantitative Determination ◽

Human Plasma ◽

Active Metabolite ◽

High Performance ◽

Limit Of Quantification ◽

Carry Over ◽

Pharmacologically Active ◽

Effective Drugs ◽

Active Substances

Introduction. Lappaconitine is an alkaloid, contained into Aconitum leucostomum Vorosh. roots and herbs. The alkaloid is indicated to arrhythmia. The lappaconitine drugs are metabolized into eight pharmacologically active substances, but N-desacetyllappaconitine is the most effective. Drugs based on a lappaconitine has narrow therapeutic range and many kinds of side effects. Pharmacokinetics of lappaconitine should be more studied for safety medical use of lappaconitine drugs.Aim. The aim of this study is to develop method for the quantitative determination of lappaconitine and its active metabolite N-desacetyllappaconitine in human plasma and blood by high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). Materials and methods. Determination of lappaconitine and N-desacetyllappaconitine in plasma and blood was carried out by HPLC-MS/MS. The samples were processed by acetonitrile protein precipitation.Results and discussion. This method was validated by next parameters: selectivity, matrix effect, calibration curve, accuracy, precision, spike recovery, lower limit of quantification, carry-over effect and stability.Conclusion. The method of the quantitative determination of lappaconitine and N-desacetyllappaconitine in human plasma and blood was developed and validated by HPLC-MS/MS. The analytical range of the was 0.50-50.00 ng/ml for lappaconitine and 0.50-100.00 ng/ml for N-desacetyllappaconitine in biological matrix. Method could be applied to determination of lappaconitine and N-desacetyllappaconitine for PK studies.

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