scholarly journals Establishment of Beauveria bassiana isolates as endophytes in rice cultivars and their biocontrol efficacy against rice stem borer, Sesamia calamistis

2020 ◽  
Vol 116 (11/12) ◽  
Author(s):  
Wonroo B.A. Bancole ◽  
Mark D. Laing ◽  
Kwasi S. Yobo ◽  
Abou Togola
Keyword(s):  
Beauveria Bassiana ◽  
Stem Borer ◽  
Rice Cultivar ◽  
Sesamia Calamistis ◽  
Biocontrol Agents ◽  
Inoculation Methods ◽  
Plant Parts ◽  
Inoculation Method ◽  
Beauveria Bassiana Isolates ◽  
Over Time

Possible endophytic colonisation of rice cultivar parts (leaves, stem and roots) by Beauveria bassiana isolates and their potential as biocontrol agents against Sesamia calamistis Hampson (African pink stem borer) were investigated. Five promising B. bassiana isolates were evaluated for their endophytic colonisation, the effectiveness of the inoculation methods and the efficacy of the isolates as biocontrol agents against S. calamistis. The plant part colonised is often dependent on the inoculation method. Colonisation of plant parts was assessed at 30 and 60 days after seed inoculation and foliar spray. For the pathogenicity activity, third instar larvae of S. calamistis were fed with rice stems that were previously inoculated with endophytic isolates of B. bassiana. Both inoculation methods led to the colonisation of the rice cultivar tissues, but were affected by the interactions of cultivars x isolates x inoculation methods. The colonisation of the cultivar plant parts varied over time (30- and 60-day intervals), and was affected by the inoculation method used. For both inoculation methods, highly significant differences were observed in the roots and the leaves over time (p=0.0001). However, with seed treatment, there was no significant difference in levels of colonisation in stems by the isolates x time (p=0.32). The B. bassiana isolates were pathogenic on the third instar larvae of S. calamistis, causing mortalities of more than 50% at 28 days after treatment. However, the virulence of the isolates varied. According to the isolates and the inoculation methods, B. bassiana formed an endophytic relationship with rice plants, and produced various mortality rates.

scholarly journals The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

2007 ◽  
Vol 12 (2) ◽  
Author(s):  
M. Estrada ◽  
Manuel Camacho ◽  
César Benito
Keyword(s):  
Beauveria Bassiana ◽  
The Other ◽  
Caribbean Region ◽  
Pcr Markers ◽  
Issr Primer ◽  
Geographic Origins ◽  
The Caribbean ◽  
Beauveria Bassiana Isolates ◽  
High Level ◽  
Issr Pcr

AbstractInter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

scholarly journals Transmission, In Planta Distribution, and Management of Hibiscus latent Fort Pierce virus, a Novel Tobamovirus Isolated from Florida Hibiscus

Plant Disease ◽  
2004 ◽  
Vol 88 (6) ◽  
pp. 674-679 ◽  
Author(s):  
Ivanka Kamenova ◽  
Scott Adkins
Keyword(s):  
Sodium Hypochlorite ◽  
Transmission Efficiency ◽  
Infection Process ◽  
Infection Rates ◽  
In Planta ◽  
Plant Propagation ◽  
Inoculation Methods ◽  
Dry Milk ◽  
Virus Distribution ◽  
Over Time

Three aspects of the infection process of a new tobamovirus species, Hibiscus latent Fort Pierce virus, recently isolated from hibiscus in Florida, were examined: (i) transmission efficiency of rub-, slash-, and cut-inoculation for two hibiscus cultivars, Pink Versicolor and Brilliant Red; (ii) distribution within infected hibiscus plants; and (iii) treatments to prevent infection during plant propagation and pruning. Rub-, slash-, and cut-inoculation methods were all effective and yielded infection rates of 66, 74, and 70%, respectively, in Pink Versicolor and 50, 56, and 38%, respectively, in Brilliant Red. Analysis of virus distribution in infected plants over time revealed that the virus moved from the place of inoculation to the roots and then toward the bottom (oldest) leaves of the plants. Virus was found in all leaves on branches of Brilliant Red plants at 210 days postinoculation, whereas it remained restricted to the bottom and middle leaves of Pink Versicolor plants at 290 days postinoculation. Although several treatments of tools reduced infection of hibiscus during experiments mimicking plant propagation and pruning, 10% (wt/vol) sodium hypochlorite and 20% (wt/vol) nonfat dry milk completely prevented infection.

scholarly journals Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)

Plant Disease ◽  
2019 ◽  
Vol 103 (4) ◽  
pp. 677-684 ◽  
Author(s):  
Krishna Ghimire ◽  
Kristina Petrović ◽  
Brian J. Kontz ◽  
Carl A. Bradley ◽  
Martin I. Chilvers ◽  
...  
Keyword(s):  
Glycine Max ◽  
Dna Sequencing ◽  
South Dakota ◽  
Disease Severity ◽  
The Other ◽  
Symptom Development ◽  
Inoculation Methods ◽  
Inoculation Method ◽  
Pathogen Recovery ◽  
The U.S

One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P < 0.001) and pathogen recovery (P < 0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate × method interaction affected disease severity (P < 0.05) and pathogen recovery (P < 0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate × method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.

Characterization of Beauveria bassiana isolates from Kyrgyzstan

2019 ◽  
Vol 167 ◽  
pp. 107243
Author(s):  
Tinatin Doolotkeldieva ◽  
Saikal Bobusheva ◽  
Aijamal Kulmanbetova ◽  
Sezim Zholdoshbekova ◽  
Aygerim Amanbek Kyzy
Keyword(s):  
Beauveria Bassiana ◽  
Beauveria Bassiana Isolates
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