Population of Asian Corn Stem Borer (Ostrinia furnacalis) on F1 of Beauveria bassiana-Inoculated Corn

The stem borer (Ostrinia furnacalis Guenee) is one of the main pests of corn. The yield loss due to this pest attack reaches 20-80%. These pests are generally controlled chemically, causing various negative effects from the results of control, therefore environmentally friendly control techniques are needed, such as the use of bio-compost and bio-LOF. Bio-compost is compost combined with a consortium of bacteria Serratia marcecens, Bacillus thuringiensis and Pseudomonas fluorescens. Bio-LOF is a liquid organic fertilizer that is processed using cow feces, cow urine, Glicerida leaves and coconut husk. The research objective is to determine the effectiveness of bio-compost with several concentrations of bio-LOF to control stem borer pests in sweet corn. The research was conducted at experimental land of Payakumbuh Agricultural Polytechnic from January to June 2020. This study used a factorial completely randomized design (4x3) with 3 replications. Factor K, namely K1; Tithonia bio-compost, K2; Hay bio-compost. K3; Manure bio-compost. K4: without bio-compost. Factor D are: D1; Bio-LOF concentration 30%, D2; Bio-LOF 20%. D3: without Bio-LOF. The data obtained were analyzed for variance using a statistical program of 8.0, then continued with the dancun test α 5%. The results showed that the three types of bio-compost combined with bio-LOF could reduce the percentage of O. furnacalis pest attack on the stems and tips of sweet corn cobs. There were 5 treatment combinations with very effective criteria for coefficient of relatively control (CRC), namely. Tithonia bio-compost, Bio-LOF 30%, Hay bio-compost, bio-LOF 30%, chicken feces bio-compost, bio-LOF 30%, Straw bio-compost, Bio-LOF 20%, Without bio-compost, Bio-LOF 30%. The concentration of bio-LOF sprayed on the stems and leaves affects the effectiveness of the control where the 30% bio-LOF concentration had the very effective criteria of control, while the 20% concentration had the effective criteria of control.

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Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response

International Journal of Molecular Sciences ◽

10.3390/ijms21218215 ◽

2020 ◽

Vol 21 (21) ◽

pp. 8215

Author(s):

Raufa Batool ◽

Muhammad Jawad Umer ◽

Yangzhou Wang ◽

Kanglai He ◽

Tiantao Zhang ◽

...

Keyword(s):

Immune Response ◽

Synergistic Effect ◽

Beauveria Bassiana ◽

Larval Mortality ◽

Seed Coating ◽

Trichoderma Asperellum ◽

Ostrinia Furnacalis ◽

Transcriptomic Response ◽

Corn Borer ◽

Fungal Isolates

Ostrinia furnacalis, is the major pest of maize causing significant yield losses. So far, many approaches have been used to increase the virulence of entomopathogenic fungal isolates. The current study is an attempt to estimate synergistic effect of Beauveria bassiana and Trichoderma asperellum in order to explore larval immune response through RNA sequencing and differentially expression analysis. In vivo synergism was examined in seven proportions (B. bassiana: T. asperellum = 1:1, 1:2, 1:3, 1:4, 4:1, 3:1, 2:1) and in the in vitro case, two inoculation methods were applied: seed coating and soil drenching. Results revealed significant decrease in plant damage and high larval mortality in fungal treatments. Fungal isolates mediated the plant defense by increasing proline, superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and protease activities. Seed coating method was proved to be the most effective in case of maize endophytic colonization. In total, 59 immune-related differentially expressed genes DEGs were identified including, cytochrome P450, heat shock protein, ABC transporter, cadherin, peptidoglycan recognition protein (PGRP), cuticlular protein, etc. Further, transcriptomic response was confirmed by qRT-PCR. Our results concluded that, coculture of B. bassiana and T. asperellum has the synergistic potential to suppress the immune response of O. furnacalis and can be used as sustainable approach to induce plant resistance through activation of defense-related enzymes.

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Biological control of the spotted stem borer Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) with the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae

SINET Ethiopian Journal of Science ◽

10.4314/sinet.v30i1.18284 ◽

2008 ◽

Vol 30 (1) ◽

Author(s):

T Tefera ◽

K L Pringle

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Chilo Partellus ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Stem Borer ◽

Spotted Stem Borer

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RNA sequencing analysis of Beauveria bassiana isolated from Ostrinia furnacalis identifies the pathogenic genes

Microbial Pathogenesis ◽

10.1016/j.micpath.2019.03.014 ◽

2019 ◽

Vol 130 ◽

pp. 190-195

Author(s):

Zhengkun Zhang ◽

Yang Lu ◽

Wenjing Xu ◽

Qian Du ◽

Li Sui ◽

...

Keyword(s):

Rna Sequencing ◽

Beauveria Bassiana ◽

Sequencing Analysis ◽

Ostrinia Furnacalis ◽

Pathogenic Genes

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Effectiveness of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. for control of the stem borer Chilo partellus (Swinhoe) in maize in Kenya

Crop Protection ◽

10.1016/0261-2194(93)90123-z ◽

1993 ◽

Vol 12 (8) ◽

pp. 601-604 ◽

Cited By ~ 12

Author(s):

Nguya K. Maniania

Keyword(s):

Beauveria Bassiana ◽

Chilo Partellus ◽

Entomopathogenic Fungus ◽

Stem Borer ◽

Fungus Beauveria Bassiana

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TINGKAT DAN POLA DISTRIBUSI INFESTASI PENGGEREK BATANG JAGUNG OSTRINIA FURNACALIS (LEPIDOPTERA: CRAMBIDAE) DI PADANGSIDIMPUAN

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v6i1.2093 ◽

2019 ◽

Vol 6 (1) ◽

pp. 25

Author(s):

Muhammad Agung Permadi ◽

Qorry Hilmiyah Harahap

Keyword(s):

East Asia ◽

Central Asia ◽

Random Distribution ◽

Stem Borer ◽

Ostrinia Furnacalis ◽

Infested Area ◽

Patterns Of Distribution ◽

Corn Field ◽

Cultivation Practices

Ostrinia furnacalis Guenee as known as Asian corn stem borer is one of the main pests in corn cultivation practices in Indonesia. Besides Indonesia, these pests attack in other Asian regions such as Central Asia, East Asia, and also Australia. This study was aimed to measure the intensity of infestation and to analyze patterns of distribution of O. furnacalis in corn field. The observations of intensity and infestation distribusiton were held on 12 corn plantations owned by farmers in six sub-districts of Padangsidimpuan. Results showed that The lowest infested area of O. furnacalis larvae was 1.1%, while the highest was 4.31%. O. furnacalis larvae infestations at each plant in each plantations has a random distribution and the distribution was varies among corb fields.

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