Microbial properties, enzyme activities and the persistence of exogenous proteins in soil under consecutive cultivation of transgenic cottons (Gossypium hirsutum L.)

One Bacillus thuringiensis (Bt) and two stacked Bt and cowpea trypsin inhibitor (Bt + CpTI) cottons and their non-transgenic isolines were consecutively cultivated to investigate the soil persistence of Cry1Ac and CpTI proteins and their effects on microbial properties and enzyme activities involving C, N, P, and S cycling in soil. Results showed that there were the persistence of Cry1Ac and CpTI proteins in soil under 4-year consecutive cultivation of transgenic cottons. Cry1Ac proteins varied from 6.75 ng/g to 12.01 ng/g and CpTI proteins varied from 30.65 to 43.60 ng/g. However, neither of these two proteins was detected in soil under non-transgenic cottons. Soil microbial biomass carbon, microbial activities, and soil enzyme activities (except urease and phosphodiesterase) significantly decreased in soil under transgenic cottons. Correlation analysis showed that most of microbial properties and enzyme activities in soil had a negative relationship with Cry1Ac content, while most of them had a positive relationship with CpTI content. Our data indicate that consecutive cultivation by genetically modified cottons with Bt and CpTI genes can result in persistence of Cry1Ac and CpTI proteins and negatively affect soil microbial and biochemical properties.

Download Full-text

Characteristics of soil microbial biomass carbon, nitrogen and enzyme activities in Picea asperata plantations with different ages in subalpine of western Sichuan, China

Acta Ecologica Sinica ◽

10.5846/stxb202008192158 ◽

2021 ◽

Vol 41 (14) ◽

Author(s):

罗明霞，胡宗达，刘兴良，李亚非，胡璟，欧定华，吴德勇 LUO Mingxia

Keyword(s):

Microbial Biomass ◽

Enzyme Activities ◽

Soil Microbial Biomass ◽

Microbial Biomass Carbon ◽

Biomass Carbon ◽

Western Sichuan ◽

Soil Microbial ◽

Soil Microbial Biomass Carbon ◽

Picea Asperata ◽

Different Ages

Download Full-text

Selected Soil Enzyme Activities, Soil Microbial Biomass Carbon, and Root Yield as Influenced by Organic Production Systems in Sweet Potato

Communications in Soil Science and Plant Analysis ◽

10.1080/00103624.2012.756506 ◽

2013 ◽

Vol 44 (8) ◽

pp. 1322-1339 ◽

Cited By ~ 2

Author(s):

M. Nedunchezhiyan ◽

G. Byju ◽

S. N. Dash ◽

N. Ranasingh

Keyword(s):

Microbial Biomass ◽

Soil Microbial Biomass ◽

Production Systems ◽

Microbial Biomass Carbon ◽

Soil Enzyme ◽

Organic Production ◽

Biomass Carbon ◽

Soil Enzyme Activities ◽

Root Yield ◽

Soil Microbial

Download Full-text

Changes in Soil Microbial Biomass, Community Composition, and Enzyme Activities After Half-Century Forest Restoration in Degraded Tropical Lands

Forests ◽

10.3390/f10121124 ◽

2019 ◽

Vol 10 (12) ◽

pp. 1124 ◽

Cited By ~ 1

Author(s):

Huiling Zhang ◽

Xin Xiong ◽

Jianping Wu ◽

Jianqi Zhao ◽

Mengdi Zhao ◽

...

Keyword(s):

Microbial Biomass ◽

Enzyme Activities ◽

Soil Microbial Biomass ◽

Extracellular Enzymes ◽

Soil Depth ◽

Microbial Properties ◽

Soil Microbial ◽

Eucalyptus Forest ◽

Different Seasons ◽

Microbial Groups

Soil carbon (C) sequestration and stabilization are determined by not only the C input to the soil but also the decomposition rate of soil organic matter (SOM), which is mainly mediated by soil microbes. Afforestation, an effective practice to restore forests from degraded or bare lands, may alter soil microbial properties, and thus soil C and nitrogen (N) dynamics. The aim of this study was to investigate the impacts of different afforestation strategies on soil microbial compositions and activities after afforestation for half a century. Soil samples were collected from two afforested sites (i.e., a restored secondary forest (RSF) and a managed Eucalyptus forest (MEP)) and two reference sites (i.e., a nearby undisturbed forest (UF), representing the climax vegetation and a bare land (BL), representing the original state before restoration) in south China. We quantified the soil microbial biomass, microbial community compositions, and activities of nine extracellular enzymes at different soil depths and in different seasons. Results showed that the soil microbial biomass, all the main soil microbial groups, and the activities of all extracellular enzymes were significantly increased after afforestation compared to the BL sites, while the ratios of fungi/bacteria (F/B), specific enzyme activities, and the ecoenzymatic stoichiometry were significantly decreased regardless of the season and soil depth. Between the two afforested sites, these microbial properties were generally higher in the RSF than MEP. However, the microbial properties in the RSF were still lower than those in the UF, although the differences varied with different seasons, soil depths, and microbial groups or enzymes. Our findings demonstrated that afforestation might significantly improve microbial properties. Afforestation is more effective in mixed-species plantation than in the monoculture Eucalyptus plantation but needs a much longer time to approach an equivalent level to the primary forests.

Download Full-text