scholarly journals Genetic diversity among asexual and sexual progenies of Phytophthora capsici detected with ISSR markers

2021 ◽  
Vol 57 (No. 4) ◽  
pp. 271-278
Author(s):  
Ping Li ◽  
Dong Liu
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Similarity ◽  
Phytophthora Capsici ◽  
Issr Markers ◽  
Similarity Coefficients ◽  
Sexual Progeny ◽  
Sexual Offspring ◽  
Issr Primers

The population structure of Phytophthora capsici among asexual and sexual progenies was analyzed using ISSR. Thirty asexual progenies of one parent and three sexual populations were assayed for genetic diversity using 5 ISSR primers and DNA from 120 offspring of P. capsici was amplified. In total, 71 reproducible ISSR fragments were obtained, of which 100% were polymorphic, revealing high polymorphism among the isolates. Among them, the percentages of polymorphism of sexual and asexual progeny isolates were 100.00 and 77.46%, respectively. Genetic similarity coefficients among all the isolates ranged from 0.54 to 0.73. The sexual offspring population showed much more variability than the asexual offspring population with 76.26% variability attributed to diversity within populations as compared with 23.74% among populations. This research reveals that the sexual progeny population of P. capsici contributes more genetic diversity than that of asexual progeny population.

Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers

Biologia ◽  
2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Guo-Ye Guo ◽  
Rui-Wu Yang ◽  
Chun-Bang Ding ◽  
Li Zhang ◽  
Yong-Hong Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Issr Markers ◽  
Similarity Coefficients ◽  
Triticeae Species ◽  
Inter Simple Sequence Repeats ◽  
Great Genetic Diversity ◽  
Simple Sequence ◽  
Issr Primers

AbstractTo investigate the genetic diversity and phylogenetic relationships between polyploid Leymus and related diploid species of the Triticeae tribe, inter-simple sequence repeats (ISSR) markers was used to analyze 41 Leymus accessions representing 22 species and 2 subspecies, together with Pseudoroegneria stipifolia (St), Psathyrostachys fragilis (Ns), Australopyrum retrofractum (W), Hordeum bogdanii, H. chilense (H) and Lophopyrum elongatum (Ee). A total of 376 clear and reproducible DNA fragments were amplified by 29 ISSR primers, among which 368 (97.87%) fragments were found to be polymorphic. 8–18 polymorphic bands were amplified by each polymorphic primer, with an average of 12.69 bands. The data of 376 ISSR bands were used to generate Nei’s similarity coefficients and to construct a dendrogram by means of UPGMA. The similarity coefficients data suggested great genetic diversity in genus Leymus and related diploid Triticeae species, the genetic diversity among the different species more abundant than that of the different accessions. The dendrogram and principal coordinate analysis showed explicit interspecific relationships and demonstrated close phylogenetic relationships between Leymus species and Psathyrostachys.

scholarly journals Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers

2020 ◽  
Vol 50 (3) ◽  
pp. 204-212
Author(s):  
Stalin Juan Vasquez GUIZADO ◽  
Muhammad Azhar NADEEM ◽  
Fawad ALI ◽  
Muzaffer BARUT ◽  
Ephrem HABYARIMANA ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Diversity ◽  
Population Level ◽  
Diversity Indices ◽  
Issr Markers ◽  
Neighbor Joining ◽  
Peruvian Amazon ◽  
Over Exploitation ◽  
Issr Primers

ABSTRACT Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed the presence of maximum variability within populations (88%). The Structure algorithm, neighbor joining and principal coordinate analysis (PCoA) grouped the 90 rosewood accessions into three main populations (A, B and C). Diversity indices at the inter-population level revealed a greater genetic diversity in population A, due to higher gene flow. The neighbor-joining analysis grouped populations A and B, while population C was found to be divergent at the inter population level. We concluded that population A reflects higher genetic diversity and should be prioritized for future management and conservation plans.

scholarly journals Study of genetic diversity and molecular variation in natural populations of Pinus armandii subsp. Xuannhaensis L.K. Phan - an endemic species in Son La, Vietnam using ISSR markers

2016 ◽  
Vol 14 (3) ◽  
pp. 441-450 ◽  
Author(s):  
Vũ Đình Duy ◽  
Bùi Thị Tuyết Xuân ◽  
Đỗ Thị Phương Thảo ◽  
Phan Kế Lộc ◽  
Nguyễn Minh Tâm
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Nature Reserve ◽  
Natural Populations ◽  
Population Level ◽  
Issr Markers ◽  
Similarity Matrix ◽  
Similarity Coefficients ◽  
Analysis Of Molecular Variance ◽  
Narrow Endemic

Pinus armandii subsp. xuannhaensis L.K. Phan is a new five needle pine discovered recently from Xuan Nha Nature Reserve, Son La province. This subspecies is considered as a narrow endemic to Vietnam and is assessed as endangered. In this study, 15 ISSR markers were used to analyze the genetic diversity of this taxon collected in five subpopulations (Tan Xuan, Thac Nuoc, Dinh VTV2, Near VTV2 and Dinh Pomu). Results of the analysis showed 15/15 markers were polymorphic. A total of 51 DNA fragments were amplified, in which 50 fragments were polymorphic (98.04%). Genetic diversity was the highest in Dinh Pomu subpopulation (I = 0.555; h = 0.8; PPB = 68.76%; Ne =1.6 and He = 0.4)) and the lowest in Tan Xuan subpopulation (I = 0.428; h = 0.6; PPB = 57.06%, Ne = 1.215 and He = 0.303). Analysis of molecular variance (AMOVA) results showed that the total level of molecular changes between subpopulations was 7% and between individuals in the same subpopulation was 93%. A constructed dendrogram based on similarity matrix of 71 Pinus armandii subsp. xuannhaensis L.K. Phan samples divided the samples into two main groups with genetic similarity coefficients ranged from 0.53 to 0.96. Results of the molecular analysis showed that Pinus armandii subsp. xuannhaensis L.K. Phan species should be protected at the population level.

Genetic relationships of Chinese prickly ash as revealed by ISSR markers

Biologia ◽  
2015 ◽  
Vol 70 (1) ◽  
Author(s):  
Shijing Feng ◽  
Tuxi Yang ◽  
Xiao Li ◽  
Lv Chen ◽  
Zhenshan Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationship ◽  
Genetic Divergence ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Chinese Prickly Ash ◽  
New Cultivars ◽  
Issr Primers

AbstractChinese prickly ash, belonging to the genus Zanthoxylum L., has been one of the most important commerciallyexploited plants for its alimentary, industrial and medicinal applications. However, the breeding and promotion of Chinese prickly ash have been severely restricted due to its confusing classification. Therefore, we assessed genetic diversity and phylogenetic relationship among 45 Chinese prickly ash samples collected from 6 main cultivated regions using 11 ISSR primers. These eleven selected primers generated a total of 102 scorable bands ranging from 150 to 2000 bp, corresponding to an average of approximately 9.3 bands per primer. The percentage of polymorphic loci for all samples ranged from 75% to 100%, with an average of 84.3%. The genetic similarity coefficient across all samples varied from 0.460 to 0.919. Remarkably, UPGMA analysis showed that 45 samples were divided into six clusters with a genetic similarity of 0.7. The closest genetic relationship was observed between Dahongpao collected from Qin’an and Tianshui, and the greatest genetic divergence was found between Dahongpao collected from Hengshui and Jiuyeqing collected from Jiangjin. It could serve as a basis for identifying Chinese prickly ash cultivars, breeding new cultivars and protecting the Chinese prickly ash resources in main regions.

Genetic diversity and population structure of the mangrove lime (Merope angulata) in India revealed by AFLP and ISSR markers

2015 ◽  
Vol 120 ◽  
pp. 260-267 ◽  
Author(s):  
Satya Narayan Jena ◽  
Sushma Verma ◽  
Kuttan Narayanan Nair ◽  
Awadhesh Kumar Srivastava ◽  
Sujata Misra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Issr Markers

scholarly journals Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers

2014 ◽  
Vol 13 (3) ◽  
pp. 6340-6349 ◽  
Author(s):  
D.X. Chen ◽  
L.Y. Li ◽  
X. Zhang ◽  
Y. Wang ◽  
Z. Zhang
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Issr Markers

scholarly journals Evaluation of a Diverse, Worldwide Collection of Wild, Cultivated, and Landrace Pepper (Capsicum annuum) for Resistance to Phytophthora Fruit Rot, Genetic Diversity, and Population Structure

2015 ◽  
Vol 105 (1) ◽  
pp. 110-118 ◽  
Author(s):  
R. P. Naegele ◽  
A. J. Tomlinson ◽  
M. K. Hausbeck
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Phytophthora Capsici ◽  
The United States ◽  
Fruit Rot ◽  
Sources Of Resistance ◽  
Plant Introductions ◽  
Genetic Clusters ◽  
Commercial Breeding ◽  
Moderately Resistant

Pepper is the third most important solanaceous crop in the United States and fourth most important worldwide. To identify sources of resistance for commercial breeding, 170 pepper genotypes from five continents and 45 countries were evaluated for Phytophthora fruit rot resistance using two isolates of Phytophthora capsici. Genetic diversity and population structure were assessed on a subset of 157 genotypes using 23 polymorphic simple sequence repeats. Partial resistance and isolate-specific interactions were identified in the population at both 3 and 5 days postinoculation (dpi). Plant introductions (PIs) 640833 and 566811 were the most resistant lines evaluated at 5 dpi to isolates 12889 and OP97, with mean lesion areas less than Criollo de Morelos. Genetic diversity was moderate (0.44) in the population. The program STRUCTURE inferred four genetic clusters with moderate to very great differentiation among clusters. Most lines evaluated were susceptible or moderately susceptible at 5 dpi, and no lines evaluated were completely resistant to Phytophthora fruit rot. Significant population structure was detected when pepper varieties were grouped by predefined categories of disease resistance, continent, and country of origin. Moderately resistant or resistant PIs to both isolates of P. capsici at 5 dpi were in genetic clusters one and two.

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