scholarly journals Results of in vitro chemotherapy of apple cv. Fragrance – Short communication

2013 ◽  
Vol 40 (No. 4.) ◽  
pp. 186-190 ◽  
Author(s):  
F. Parštein ◽  
J. Sedlák ◽  
L. Svobodová ◽  
J. Polák ◽  
S. Gadiou
Keyword(s):  
Repeated Treatment ◽  
Pome Fruit ◽  
Initial Field ◽  
In Vitro Plants ◽  
Apple Stem ◽  
In Vitro Shoots ◽  
Chlorotic Leaf ◽  
Stem Pitting ◽  
In Vitro Chemotherapy

The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity.

scholarly journals The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 13-17 ◽  
Author(s):  
J.K. Kundu
Keyword(s):  
Mixed Infection ◽  
Rt Pcr ◽  
Pome Fruit ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Apple Cultivars ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard. These two main pome fruit viruses were detected frequently in all tested apple cultivars. ASGV and ASPV occurred in as many as 16 trees (in the cultivar Spartan) and 13 trees (in the cultivar Idared) out of 20 tested trees, respectively. Mixed infection by ASGV and ASPV was found in all tested cultivars (as many as 9 out of 20 tested trees of the cultivar Spartan).

Distribution of apple stem grooving virus and apple chlorotic leaf spot virus in infected in vitro pear shoots

2010 ◽  
Vol 29 (12) ◽  
pp. 1447-1451 ◽  
Author(s):  
L.P. Wang ◽  
N. Hong ◽  
G.P. Wang ◽  
W.X. Xu ◽  
R. Michelutti ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf

scholarly journals Gospodarski važni virusi jezgričavog voća

2019 ◽  
Vol 22 (3-4) ◽  
pp. 123-136
Author(s):  
Dario Ivić
Keyword(s):  
Mosaic Virus ◽  
Leaf Spot ◽  
Spot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Apple Stem Pitting Virus ◽  
Stem Pitting

Virusi jabuke, kruške ili dunje relativno su slabo poznati stručnjacima i voćarima. Najvažnijim virusima koji se javljaju na jezgričavim voćnim vrstama smatraju se virus mozaika jabuke (Apple mosaic virus, ApMV), virus klorotične pjegavosti lista jabuke (Apple chlorotic leaf spot virus, ACLSV), virus brazdavosti debla jabuke (Apple stem grooving virus, ASGV) i virus jamičavosti debla jabuke (Apple stem pitting virus,ASPV). U radu je ukratko opisana njihova važnost, biologija i regulativni status, kao i osnovne mjere zaštite.

scholarly journals Survey for viruses and viroids in apple and pear trees in New Zealand

2010 ◽  
Vol 63 ◽  
pp. 281-281
Author(s):  
M.B. Horner
Keyword(s):  
New Zealand ◽  
Spot Virus ◽  
Tomato Ringspot Virus ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Chlorotic Leaf ◽  
Pear Trees ◽  
Leaf Virus ◽  
Stem Pitting ◽  
Geographical Locations

Surveys to determine the phytosanitary status of apple (Malus) and pear (Pyrus) trees in New Zealand were conducted from 2005 to 2006 A total of 188 symptomatic and nonsymptomatic trees from various geographical locations were tested for the presence of a number of viruses and viroids by RTPCR All Malus samples were tested for Apple chlorotic leaf spot virus (ACLSV) Apple stem grooving virus (ASGV) Apple stem pitting virus (ASPV) Apple mosaic virus (ApMV) Apple scar skin viroid (ASSVd) Apple dimple fruit viroid (ADFVd) Cherry rasp leaf virus (CRLV) and Tomato ringspot virus (ToRSV) ACLSV was detected in 48 ASGV in 36 ASPV in 61 and ApMV in 45 of samples tested ASSVd ADFVd CRLV ToRSV were not detected in any of the 165 sampled Malus plants which provides evidence that they are not present in New Zealand All Pyrus samples were tested for ACLSV ASGV ASPV ApMV Pear latent virus (PeLV) and Pear blister canker viroid (PBCVd) ACLSV was detected in 17 ASGV in 13 ASPV in 17 and ApMV in 4 of sampled Pyrus trees PeLV and PBCVd were not detected in any of the 23 sampled symptomatic Pyrus trees which provides evidence that PeLV and PBCVd are not present in New Zealand

scholarly journals Simplification of RNA preparation procedure for RT-PCR in detection of pome fruit tree viruses

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 252-254 ◽  
Author(s):  
J.K. Kundu
Keyword(s):  
Rna Extraction ◽  
Similar Rate ◽  
Preparation Procedure ◽  
Plant Tissues ◽  
Rt Pcr ◽  
Pome Fruit ◽  
Apple Stem Grooving Virus ◽  
Apple Stem ◽  
Apple Cultivars ◽  
Stem Pitting

A rapid, easy to handling and sensitive RNA preparation procedure, RNA release protocol was described here for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) by RT-PCR. Comparing total RNA extraction protocol, RNA release protocol give raised similar rate of ASPV and ASGV detection within the field-grown apple cultivars. Among sampling plant tissues, the bud leaf and leaf (during blossom) were showed efficient tissues for the routine detection, regardless the using RNA preparation procedures.

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