scholarly journals Different impacts of an electron shuttle on nitrate- and nitrite-dependent anaerobic oxidation of methane in paddy soil

2021 ◽  
Vol 67 (No. 5) ◽  
pp. 264-269
Author(s):  
Yaohong Zhang ◽  
Fangyuan Wang
Keyword(s):  
Organic Matter ◽  
Paddy Soil ◽  
Anaerobic Oxidation Of Methane ◽  
Electron Shuttle ◽  
Anaerobic Oxidation ◽  
Terminal Electron Acceptor ◽  
Electron Shuttles ◽  
Oxidation Of Methane ◽  
Nitrate And Nitrite ◽  
C Assimilation

Quinones, redox-active functional groups in soil organic matter, can act as electron shuttles for microbial anaerobic transformation. Here, we used <sup>13</sup>CH<sub>4</sub> to trace <sup>13</sup>C conversion (<sup>13</sup>C-CO<sub>2</sub> + <sup>13</sup>C-SOC) to investigate the influence of an artificial electron shuttle (anthraquinone-2,6-disulfonate, AQDS) on denitrifying anaerobic methane oxidation (DAMO) in paddy soil. The results showed that AQDS could act as the terminal electron acceptor for the anaerobic oxidation of methane (AOM) in the paddy field. Moreover, AQDS significantly enhanced nitrate-dependent AOM rates and the amount of <sup>13</sup>C-CH<sub>4</sub> assimilation to soil organic carbon (SOC), whereas it was remarkably reduced nitrite-dependent AOM rates and <sup>13</sup>C assimilation. Ultimately, AQDS notably increased the total DAMO rates and <sup>13</sup>C assimilation to SOC. However, the electron shuttle did not change the percentage of <sup>13</sup>C-SOC in total <sup>13</sup>C-CH<sub>4</sub> conversion. These results suggest that electron shuttles in the natural organic matter might be able to offset methane emission by facilitating AOM coupled with the denitrification process.

Electron shuttles enhance anaerobic oxidation of methane coupled to iron(III) reduction

2019 ◽  
Vol 688 ◽  
pp. 664-672 ◽  
Author(s):  
Qiuxiang He ◽  
Linpeng Yu ◽  
Jibing Li ◽  
Dan He ◽  
Xixi Cai ◽  
...  
Keyword(s):  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Electron Shuttles ◽  
Oxidation Of Methane

scholarly journals Anaerobic Oxidation of Methane in Sediments of Lake Constance, an Oligotrophic Freshwater Lake

2011 ◽  
Vol 77 (13) ◽  
pp. 4429-4436 ◽  
Author(s):  
Jörg S. Deutzmann ◽  
Bernhard Schink
Keyword(s):  
Methane Oxidation ◽  
Electron Acceptors ◽  
Molecular Techniques ◽  
Freshwater Lake ◽  
Lake Constance ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Terminal Electron Acceptor ◽  
Oxidation Of Methane ◽  
Freshwater Habitats

ABSTRACTAnaerobic oxidation of methane (AOM) with sulfate as terminal electron acceptor has been reported for various environments, including freshwater habitats, and also, nitrate and nitrite were recently shown to act as electron acceptors for methane oxidation in eutrophic freshwater habitats. Radiotracer experiments with sediment material of Lake Constance, an oligotrophic freshwater lake, were performed to follow14CO2formation from14CH4in sediment incubations in the presence of different electron acceptors, namely, nitrate, nitrite, sulfate, or oxygen. Whereas14CO2formation without and with sulfate addition was negligible, addition of nitrate increased14CO2formation significantly, suggesting that AOM could be coupled to denitrification. Nonetheless, denitrification-dependent AOM rates remained at least 1 order of magnitude lower than rates of aerobic methane oxidation. Using molecular techniques, putative denitrifying methanotrophs belonging to the NC10 phylum were detected on the basis of thepmoAand 16S rRNA gene sequences. These findings show that sulfate-dependent AOM was insignificant in Lake constant sediments. However, AOM can also be coupled to denitrification in this oligotrophic freshwater habitat, providing first indications that this might be a widespread process that plays an important role in mitigating methane emissions.

Early Cretaceous glendonites of Arctic realm: distributions and their paleoclimate implication

2021 ◽  
Author(s):  
Kseniya Mikhailova ◽  
Victoria Ershova ◽  
Mikhail Rogov
Keyword(s):  
Organic Matter ◽  
Early Cretaceous ◽  
Isotope Composition ◽  
Cold Climate ◽  
The Arctic ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Arctic Canada ◽  
Oxidation Of Methane ◽  
Pee Dee

&lt;p&gt;In the middle of 20&lt;sup&gt;th&lt;/sup&gt; century glendonites were purposed as an indicator of cold climate. There is no doubt that unique morphology and sizes of pseudomorphs occurring through Precambrian to Quaternary succession indicate uncommon geochemical environment. Here, we present an overview of Early Cretaceous glendonites distribution across Arctic which widely distributed here despite generally greenhouse climate conditions in Early Cretaceous.&lt;/p&gt;&lt;p&gt;Late Berriasian pseudomorphs are known on northeastern Siberia and Arctic Canada. Valanginian glendonites are the widest ones are described from the Northern and Western Siberia, Spitsbergen and the Arctic Canada. Late Hauterivian concretions were studied on Svalbard. Barremian and lower Aptian glendonites are unknown in this area due to wide distributed continental succession, but late Barremian glendonites were reported from the wells drilled on the Barents Sea shelf. Middle and Upper Aptian glendonites are found on Svalbard, &amp;#160;North Greenland, the Arctic Canada and North-East Russia. Lower Albian glendonites are found on Svalbard, islands of Arctic Canada and the Koryak Uplands.&lt;/p&gt;&lt;p&gt;Nowadays it is reliable known that the precursor of glendonites is an ikaite - metastable calcium carbonate hexahydrate, forming in a narrow temperature range from 0-4&lt;sup&gt;o&lt;/sup&gt;C, mainly in near-bottom conditions. Besides low temperature, high phosphate concentrations that occurs due to anaerobic oxidation of methane and/or organic matter; dissolved organic carbon, sulfates and amino acid may favor to ikaite formation as well. However, glendonites associated with terrigenious rocks, often including glacial deposits, that allow to use them as a paleoclimate indicator.&lt;/p&gt;&lt;p&gt;Glendonites show a wide variability in form and size: from single crystal blades to stellate aggregates and rosettes, usually ranged from a few mm to dozens of cm. Mineralogical composition of pseudomorph is represented mainly by three calcite phases determining by CL-light. Both &amp;#948;&lt;sup&gt;18&lt;/sup&gt;O and &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C of glendonites are characterized by a broad range of values. Oxygen isotope composition ranges from -14 to -0 &amp;#8240; Vienna Pee Dee Belemnite (VPDB), whilst &amp;#160;carbon isotope composition ranges from -52.4 to &amp;#8211; 14 &amp;#8240; Vienna Pee Dee Belemnite (VPDB).&lt;/p&gt;&lt;p&gt;Based on received data we suggest that &amp;#948;&lt;sup&gt;18&lt;/sup&gt;O reflects the complex processes involved in ikaite-glendonite transformation, supposing mixing depleted fluids with seawater. Nevertheless, received data coincide with &amp;#948;&lt;sup&gt;18&lt;/sup&gt;O values reported from Paleozoic-Quaternary glendonites formed in near-freezing environments. Values of &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C of glendonites is the result of both mixing seawater inorganic carbon and sedimentary organic diagenesis and close to bacterial sulfate reduction and/or anaerobic oxidation of methane or organic matter.&lt;/p&gt;&lt;p&gt;To conclude,&amp;#160; Early Cretaceous climate was warm generally, however studied pseudomorphs point to cold episodes in Late Berriasian, Valanginian, Late Hauterivian, Middle-Late Aptian and Early Albian.&lt;/p&gt;&lt;p&gt;The study was supported by RFBR, project number 20-35-70012.&lt;/p&gt;

scholarly journals Anaerobic Methane Oxidation Driven by Microbial Reduction of Natural Organic Matter in a Tropical Wetland

2017 ◽  
Vol 83 (11) ◽  
Author(s):  
Edgardo I. Valenzuela ◽  
Alejandra Prieto-Davó ◽  
Nguyen E. López-Lozano ◽  
Alberto Hernández-Eligio ◽  
Leticia Vega-Alvarado ◽  
...  
Keyword(s):  
Organic Matter ◽  
Natural Organic Matter ◽  
Methane Emissions ◽  
Microbial Reduction ◽  
Electron Acceptors ◽  
Anaerobic Oxidation Of Methane ◽  
Wetland Sediment ◽  
Terminal Electron Acceptor ◽  
Oxidation Of Methane ◽  
Humic Fraction

ABSTRACT Wetlands constitute the main natural source of methane on Earth due to their high content of natural organic matter (NOM), but key drivers, such as electron acceptors, supporting methanotrophic activities in these habitats are poorly understood. We performed anoxic incubations using freshly collected sediment, along with water samples harvested from a tropical wetland, amended with 13C-methane (0.67 atm) to test the capacity of its microbial community to perform anaerobic oxidation of methane (AOM) linked to the reduction of the humic fraction of its NOM. Collected evidence demonstrates that electron-accepting functional groups (e.g., quinones) present in NOM fueled AOM by serving as a terminal electron acceptor. Indeed, while sulfate reduction was the predominant process, accounting for up to 42.5% of the AOM activities, the microbial reduction of NOM concomitantly occurred. Furthermore, enrichment of wetland sediment with external NOM provided a complementary electron-accepting capacity, of which reduction accounted for ∼100 nmol 13CH4 oxidized · cm−3 · day−1. Spectroscopic evidence showed that quinone moieties were heterogeneously distributed in the wetland sediment, and their reduction occurred during the course of AOM. Moreover, an enrichment derived from wetland sediments performing AOM linked to NOM reduction stoichiometrically oxidized methane coupled to the reduction of the humic analogue anthraquinone-2,6-disulfonate. Microbial populations potentially involved in AOM coupled to microbial reduction of NOM were dominated by divergent biota from putative AOM-associated archaea. We estimate that this microbial process potentially contributes to the suppression of up to 114 teragrams (Tg) of CH4 · year−1 in coastal wetlands and more than 1,300 Tg · year−1, considering the global wetland area. IMPORTANCE The identification of key processes governing methane emissions from natural systems is of major importance considering the global warming effects triggered by this greenhouse gas. Anaerobic oxidation of methane (AOM) coupled to the microbial reduction of distinct electron acceptors plays a pivotal role in mitigating methane emissions from ecosystems. Given their high organic content, wetlands constitute the largest natural source of atmospheric methane. Nevertheless, processes controlling methane emissions in these environments are poorly understood. Here, we provide tracer analysis with 13CH4 and spectroscopic evidence revealing that AOM linked to the microbial reduction of redox functional groups in natural organic matter (NOM) prevails in a tropical wetland. We suggest that microbial reduction of NOM may largely contribute to the suppression of methane emissions from tropical wetlands. This is a novel avenue within the carbon cycle in which slowly decaying NOM (e.g., humic fraction) in organotrophic environments fuels AOM by serving as a terminal electron acceptor.

Organic matter sulfurization on protracted diagenetic timescales: The possible role of anaerobic oxidation of methane

2016 ◽  
Vol 381 ◽  
pp. 54-66 ◽  
Author(s):  
Melesio Quijada ◽  
Armelle Riboulleau ◽  
Pierre Faure ◽  
Raymond Michels ◽  
Nicolas Tribovillard
Keyword(s):  
Organic Matter ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Oxidation Of Methane

scholarly journals Nitrate- and nitrite-dependent anaerobic oxidation of methane

2016 ◽  
Vol 8 (6) ◽  
pp. 941-955 ◽  
Author(s):  
Cornelia U. Welte ◽  
Olivia Rasigraf ◽  
Annika Vaksmaa ◽  
Wouter Versantvoort ◽  
Arslan Arshad ◽  
...  
Keyword(s):  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Oxidation Of Methane ◽  
Nitrate And Nitrite

Anaerobic oxidation of methane in paddy soil: Role of electron acceptors and fertilization in mitigating CH4 fluxes

2020 ◽  
Vol 141 ◽  
pp. 107685 ◽  
Author(s):  
Lichao Fan ◽  
Michaela A. Dippold ◽  
Tida Ge ◽  
Jinshui Wu ◽  
Volker Thiel ◽  
...  
Keyword(s):  
Paddy Soil ◽  
Electron Acceptors ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Oxidation Of Methane
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