scholarly journals The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.)

2021 ◽  
Vol 21 (2) ◽  
pp. 176-182
Author(s):  
Serap BİLGİN ◽  
Esra ALIM
2019 ◽  
Vol 49 ◽  
Author(s):  
Ángel Cabello ◽  
Nicolás Espinoza ◽  
Sergio Espinoza ◽  
Antonio Cabrera ◽  
Rómulo Santelices

Background: Nothofagus glauca (Phil.) Krasser (Nothofagaceae, “Hualo”) is an endemic tree of the Mediterranean zone of Chile. The natural forests in this area have been severely fragmented as a result of human causes such as replacement by agricultural crops and fast-growing tree species. From 1975, these forests have declined from 900,000 ha to 145,000 ha, so it is categorised on the IUCN Red List as ‘vulnerable’. In restoring this ecosystem, efforts should focus, in part, on the propagation of quality stock. However, information on propagation systems is still insufficient. Methods: We aimed to analyse the effect of different pre-germinative treatments and sowing times on seed germination, and seedling growth and quality. The pre-germinative treatments were: (i) cold stratification; (ii) soaking in gibberellic acid (GA3) and thiourea solution; and (iii) nursery cultivation, while the sowing times were July, August and September. Results: A high germination capacity was achieved by: soaking the seeds in GA3 solution irrespective of concentration; stratifying, irrespective of period; or soaking in 7.5 mg L-1 thiourea solution, values significantly varied from that of the control treatments. The sowing time was not relevant in terms of the percentage of germination or seedling development. Stratification at 5°C for 60 days produced the best quality indices for N. glauca seedlings but no significant differences were found in any of the morphological attributes tested as a result of the pre-germinative treatments. Conclusions: The pre-germinative treatments significantly improved the germination and seedlings growth of N. glauca. Cold stratification at 5°C for 60 days is recommended as it produced suitable seedlings for field establishment. Gibberellic acid and thiourea did not produce important effects on seedling growth. Our results suggest the presence of endogenous physiological dormancy of the N. glauca seeds. The results of this study provide important information on propagation and nursery techniques of N. glauca, which can be used in restoration programmes.


2000 ◽  
Vol 78 (12) ◽  
pp. 1552-1560 ◽  
Author(s):  
Carol C Baskin ◽  
Per Milberg ◽  
Lars Andersson ◽  
Jerry M Baskin

Freshly matured seeds of the dwarf shrubs Vaccinium myrtillus L., Vaccinium vitis-idaea L., and Vaccinium uliginosum L. from various sites in coniferous forests in Sweden were tested for germination before and after cold stratification. In initial tests, seeds of V. myrtillus and V. vitis-idaea germinated to 62–100% in light at 20:10 and (or) at 25:15°C with few (1–12%) or no seeds germinating at 15:5(6)°C; however, after 12 or 20 weeks of stratification germination increased significantly in light at 15:5(6)°C. Thus, seeds of these species are conditionally dormant at maturity. Of seeds sown outdoors in southern Sweden on various dates in summer, 1–20% of V. myrtillus and 0–2% of V. vitis-idaea germinated before winter. Seeds of neither species germinated during late May, when minimum temperatures were about 7–10°C, but they did germinate after minimum temperatures increased to [Formula: see text] 10°C. Although stratification increased germination percentages of V. myrtillus and V. vitis-idaea seeds at 15:5(6)°C, more than 2 weeks of incubation at this temperature was required for germination. Thus, even after habitat temperatures have reached the minimum range for germination of nondormant seeds, a slow rate of germination at low temperatures may prevent stratified seeds of both species from germinating in the field in early spring. Nonstratified seeds of V. uliginosum germinated to a maximum of 5% in light and of 7% in darkness, but after 12 weeks of stratification, seeds germinated to 49–95% in light and to 1–60% in darkness at the three temperature regimes. Most seeds of V. uliginosum are dormant at maturity, and they come out of dormancy during stratification in winter. By spring, seeds of V. uliginosum germinate over the same range of temperatures as those of V. myrtillus and V. vitis-idaea, but seeds of V. uliginosum germinate faster at 15:5(6)°C than those of the other two species.Key words: blueberry seed germination, cold stratification, Ericaceae, seed dormancy, seed germination, Vaccinium.


2016 ◽  
Author(s):  
Guan-song Yang ◽  
Liu Yang ◽  
Yue-hua Wang ◽  
Shi-kang Shen

Yunnanopilia longistaminea is an endangered monotypic species belonging to Opiliaceae. This edible plant is an important germplasm source with a high economic value in China. The seed dormancy and germination of Opiliaceae species have been rarely investigated. This study examined the effects of scarification, soaking in gibberellic acid, and dehydration on the seed germination of Y. longistaminea. Results indicated that the seed germination of this species involves two stages: radicle emergence and epicotyls (shoot) emergence. During radicle emergence, the optimum temperatures were 28 °C and 28 °C/20 °C. Seed moisture content and viability decreased as dehydration occurred. Thus, the seeds may be recalcitrant. The optimum GA3 solution for the seeds undergoing shoot emergence was 100 mg·L−1. The percentages of shoot emergence in 7 and 14 days of stratification at 5 °C were slightly higher than those in other groups. This study is the first to describe epicotyl dormancy in Y. longistaminea seeds. From the seed grow to the seedling of Y. longistaminea subjected to a autumn→winter→spring temperature process in nature conditions. Warm and cold stratification can alleviate radicle and epicotyl dormancy, respectively. The duration of cold stratification also significantly affects the epicotyl dormancy release of Y. longistaminea. The researches on the seeds breaking methods: warm(28°C/20°C)→cold(5°C)→GA3(100mg·L−1)→warm(28°C/20°C).


2017 ◽  
Vol 226 ◽  
pp. 241-249 ◽  
Author(s):  
Stefania Nin ◽  
William Antonio Petrucci ◽  
Massimo Del Bubba ◽  
Claudia Ancillotti ◽  
Edgardo Giordani

2016 ◽  
Author(s):  
Guan-song Yang ◽  
Liu Yang ◽  
Yue-hua Wang ◽  
Shi-kang Shen

Yunnanopilia longistaminea is an endangered monotypic species belonging to Opiliaceae. This edible plant is an important germplasm source with a high economic value in China. The seed dormancy and germination of Opiliaceae species have been rarely investigated. This study examined the effects of scarification, soaking in gibberellic acid, and dehydration on the seed germination of Y. longistaminea. Results indicated that the seed germination of this species involves two stages: radicle emergence and epicotyls (shoot) emergence. During radicle emergence, the optimum temperatures were 28 °C and 28 °C/20 °C. Seed moisture content and viability decreased as dehydration occurred. Thus, the seeds may be recalcitrant. The optimum GA3 solution for the seeds undergoing shoot emergence was 100 mg·L−1. The percentages of shoot emergence in 7 and 14 days of stratification at 5 °C were slightly higher than those in other groups. This study is the first to describe epicotyl dormancy in Y. longistaminea seeds. From the seed grow to the seedling of Y. longistaminea subjected to a autumn→winter→spring temperature process in nature conditions. Warm and cold stratification can alleviate radicle and epicotyl dormancy, respectively. The duration of cold stratification also significantly affects the epicotyl dormancy release of Y. longistaminea. The researches on the seeds breaking methods: warm(28°C/20°C)→cold(5°C)→GA3(100mg·L−1)→warm(28°C/20°C).


2012 ◽  
Vol 40 (2) ◽  
pp. 183 ◽  
Author(s):  
Elias PIPINIS ◽  
Elias MILIOS ◽  
Olga MAVROKORDOPOULOU ◽  
Christina GKANATSIOU ◽  
Maria ASLANIDOU ◽  
...  

Sexual propagation of Prunus mahaleb is difficult due to seed dormancy. To overcome dormancy and maximize germination, various pretreatments have been applied, including stratification (warm and cold), gibberellic acid (GA3), sulfuric acid scarification (AS), and endocarp removal. The results show that warm stratification (WS) prior to cold stratification (CS) does not improve seed germination and a long period of WS (3 months) is disastrous for germination. CS alone (up to 4 months) has been found to hasten and increase seed germination. Pretreatment of the seeds with exogenous GA3, during the CS period, has been observed to result in significantly higher seed germination. AS of seeds for 45 minutes prior to GA3 (1000 ppm for 24 hours) plus CS (up to 1 month) pretreatment has been considered to reduce the mechanical resistance of endocarp and improve germination. However, extended time of AS (180 minutes) prior to GA3 plus CS pretreatment has been found to harm the seeds. The removal of endocarp has been noted to significantly improve germination. Seeds without endocarp, which were pretreated with GA3 (1000 or 2000 ppm for 24 hours) and then cold stratified for 1 month, have been noted to exhibit the highest germination percentages.


2020 ◽  
Vol 48 (2) ◽  
pp. 289-295
Author(s):  
Valentina Mursaliyeva ◽  
Akjunus Imanbayeva ◽  
Rano Parkhatova

The results of evaluating the laboratory seed germination of endemic Allochrusa gypsophiloides (Turkestan soap root), depending on storage conditions in combination with gibberellic acid treatment (GA3), are presented. In dry storage, control seeds were characterised by a long after-ripening period and a fluctuating germination behaviour upon removal from storage, with a maximum value of 23%. The sensitivity of seeds to GA3 during dry storage varied significantly, with two germination peaks at 5-7 months, and 12 months (37.5 and 50% germination, respectively). Cold stratification and cryo-preservation accelerated seed after-ripening, promoted germination synchronisation and increased seed sensitivity to GA3. The cold stratification of seeds increased germination four months earlier than during dry storage. GA3 increased germination from 16.7 and 18.3% for the control to 37.5 and 45% for seeds cryopreserved for 5 and 12 months, respectively. We recommend cryopreserving Turkestan soap root seeds to avoid viability loss and to then germinate the seeds after pretreatment with GA3.


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