Effects of cold-stratification, gibberellic acid and potassium nitrate on seed germination of yellow gentian (Gentiana lutea L.)

2012 ◽  
Vol 11 (68) ◽  
Author(s):  
Fadil Millaku
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Potassium Nitrate ◽  
Cold Stratification ◽  
Gentiana Lutea

scholarly journals Germination of Phlox pilosa L. Seeds Is Improved by Gibberellic Acid and Light but Not Stratification, Potassium Nitrate, or Surface Disinfestation

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1263-1267 ◽  
Author(s):  
Angela M. Madeiras ◽  
Thomas H. Boyle ◽  
Wesley R. Autio
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Nitrate Concentration ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Promising Method ◽  
Cold Stratification ◽  
Additive Effects ◽  
Surface Sterilization ◽  
Growth Of Fungi

The effects of warm stratification and cold stratification, gibberellin-3 (GA3) concentration, potassium nitrate concentration, light, and duration of surface sterilization on the germination of downy phlox (Phlox pilosa L.) seeds were studied. Germination after 21 days (G21), days to 50% germination (T50), and number of days between 10% and 90% final germination (T90–T10) were calculated for each treatment. Total germination percentage was most significantly improved by cold stratification at 5 ± 2 °C for 10 weeks after warm stratification at 20 °C for 2 weeks; however, a substantial amount of germination occurred during the prestratification period, thus resulting in a crop with poor uniformity. A total of 10 mg·L−1 GA3 significantly improved the G21, T50, and T90–T10 values. Although GA3 concentration and duration of cold stratification period interacted significantly when the two were combined, the additive effects of GA3 and cold stratification did not significantly improve G21 values over those obtained with GA3 alone nor were T50 values improved over those obtained with cold stratification alone. Potassium nitrate did not influence the T50 and T90–T10 values and improved G21 only slightly. Light was found to be necessary for germination. Surface sterilization with 10% bleach decreased the growth of fungi on seeds but had no significant effect on the germination responses of P. pilosa seeds. Application of GA3 at 10 mg·L−1 is a promising method for improving seed germination in perennial Phlox species.

scholarly journals Effect of pre-germinative treatments on Nothofagus glauca seed germination and seedling growth

2019 ◽  
Vol 49 ◽  
Author(s):  
Ángel Cabello ◽  
Nicolás Espinoza ◽  
Sergio Espinoza ◽  
Antonio Cabrera ◽  
Rómulo Santelices
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Growth ◽  
Iucn Red List ◽  
Cold Stratification ◽  
Natural Forests ◽  
Sowing Time ◽  
High Germination ◽  
Thiourea Solution ◽  
Quality Stock

Background: Nothofagus glauca (Phil.) Krasser (Nothofagaceae, “Hualo”) is an endemic tree of the Mediterranean zone of Chile. The natural forests in this area have been severely fragmented as a result of human causes such as replacement by agricultural crops and fast-growing tree species. From 1975, these forests have declined from 900,000 ha to 145,000 ha, so it is categorised on the IUCN Red List as ‘vulnerable’. In restoring this ecosystem, efforts should focus, in part, on the propagation of quality stock. However, information on propagation systems is still insufficient. Methods: We aimed to analyse the effect of different pre-germinative treatments and sowing times on seed germination, and seedling growth and quality. The pre-germinative treatments were: (i) cold stratification; (ii) soaking in gibberellic acid (GA3) and thiourea solution; and (iii) nursery cultivation, while the sowing times were July, August and September. Results: A high germination capacity was achieved by: soaking the seeds in GA3 solution irrespective of concentration; stratifying, irrespective of period; or soaking in 7.5 mg L-1 thiourea solution, values significantly varied from that of the control treatments. The sowing time was not relevant in terms of the percentage of germination or seedling development. Stratification at 5°C for 60 days produced the best quality indices for N. glauca seedlings but no significant differences were found in any of the morphological attributes tested as a result of the pre-germinative treatments. Conclusions: The pre-germinative treatments significantly improved the germination and seedlings growth of N. glauca. Cold stratification at 5°C for 60 days is recommended as it produced suitable seedlings for field establishment. Gibberellic acid and thiourea did not produce important effects on seedling growth. Our results suggest the presence of endogenous physiological dormancy of the N. glauca seeds. The results of this study provide important information on propagation and nursery techniques of N. glauca, which can be used in restoration programmes.

scholarly journals Methods of breaking seed dormancy for ornamental passion fruit species

2017 ◽  
Vol 23 (1) ◽  
pp. 72 ◽  
Author(s):  
Thalita Neves Marostega ◽  
Petterson Baptista Da Luz ◽  
Armando Reis Tavares ◽  
Leonarda Grillo Neves ◽  
Severino De Paiva Sobrinho
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Potassium Nitrate ◽  
Plant Production ◽  
Commercial Plant ◽  
Fruit Species ◽  
Breaking Seed Dormancy ◽  
And Control

The Passiflora L. genus covers a diversity of wild species with ornamental potential, especially due to the intrinsic beauty of its exotic flowers, flowering more than once a year and the lush foliage. However, Passiflora seeds present dormancy complicating seed germination and the establishment of commercial plant production with species with high ornamental potential. This study was conducted to determine the best pre-germination treatments to overcome seed dormancy for Passiflora quadrangularis, P. nitida, P. foetida, P. eichleriana, P. alata, P. cincinnata, P. mucronata, P. micropetala, P. suberosa, P. morifolia and P. tenuifila. The experimental design was completely randomized, with five treatments and four replicates, with 25 seeds per plot. Pre-germination treatments were: seeds soaked in 1,000 mg L- 1 GA3 (gibberellic acid) for 6 hours, seeds soaked in 0.2 % KNO3 (potassium nitrate) for 24 hours, seeds soaked in 1 % KNO3 for 24 hours, partial seedcoat scarification with sandpaper number 120 and control (seeds untreated). Percentage of germination, germination velocity index and radicle length were evaluated for all species. The results showed that GA3 was effective to overcome seed dormancy in P. suberosa (86%), P. morifolia (68 %) and P. tenuifila (54%). KNO3 1% had significant effect on overcoming dormancy in seeds of P. eichleriana (66%) and scarification with sandpaper increased seed germination of P. micropetala (38%).

Gentiana lutea L. subsp. lutea seed germination: natural versus controlled conditions

Botany ◽  
2016 ◽  
Vol 94 (8) ◽  
pp. 653-659 ◽  
Author(s):  
Alba Cuena-Lombraña ◽  
Marco Porceddu ◽  
Caterina Angela Dettori ◽  
Gianluigi Bacchetta
Keyword(s):  
Seed Germination ◽  
Cold Stratification ◽  
Remarkable Effect ◽  
Thermal Requirements ◽  
Gentiana Lutea ◽  
Controlled Conditions ◽  
Different Temperatures ◽  
Soil Temperatures ◽  
Germination Behaviour ◽  
European Mountains

Gentiana lutea L. subsp. lutea is a geophyte that occurs in the central-southern European mountains. We investigated its germination ecophysiology with the main aim of evaluating seed germination behaviour under controlled and natural conditions. Seeds from four natural sites were tested for germination requirements under a range of different temperatures, and by comparing the results with those obtained under both cold stratification at 5 °C and treatment with GA3. We simultaneously carried out germination tests at each collecting site, and soil temperatures were recorded. Our results indicate that germination under controlled conditions is limited by high temperatures (25 °C), below which GA3 has a remarkable effect on germination percentages. Cold stratification did not have an influence on the germination response, thus suggesting that a temperature of 5 °C is not sufficient to break seed dormancy; this result was supported by soil temperatures. Gentiana lutea created a short-term persistent seed bank, and seeds mainly germinated in their first year. The germination under controlled conditions was similar in all accessions, while different responses were found in the field, where a potential influence of the aspect was observed. These findings underscore the importance of understanding germination behaviour in the field to define the real thermal requirements of a taxon.

scholarly journals Seed germination of Carex lainzii Luceño, E. Rico & T. Romero: An endemic Spanish endangered species

2019 ◽  
Vol 20 (3) ◽  
pp. 704-711
Author(s):  
KAROLINE APARECIDA FELIX RIBEIRO ◽  
CRISTIAN MADEIRA DE MEDEIROS ◽  
JOSÉ ÁNGEL SÁNCHEZ-AGUDO ◽  
JOSÉ SÁNCHEZ-SÁNCHEZ
Keyword(s):  
Seed Germination ◽  
Endangered Species ◽  
Ex Situ Conservation ◽  
Seed Viability ◽  
Potassium Nitrate ◽  
Ex Situ ◽  
Cold Stratification ◽  
Different Temperatures ◽  
Two Populations

Abstract. Ribeiro KAF, Madeira de Medeiros C, Agudo JAS, Sánchez JS. 2019. Seed germination of Carex lainzii Luceño, E. Rico & T. Romero: An endemic Spanish endangered species. Biodiversitas 20: xxxx. Strategies to halt the decline of biodiversity include: in-situ and ex-situ conservation, the latter already considered at the global level essential in conservation programs. The results of the germinative responses of Carex lainzii Luceño, E. Rico & T. Romero (Cyperaceae), an endemic Spanish endangered species, are presented in this work, to different trials carried out in the laboratory with seeds harvested in the two known populations of that community. Treatments with and without 0.2% potassium nitrate (KNO3) were tested for four, six and eight months in cold stratification at 5 °C in two germination chambers at different temperatures (22/10 °C and 27/15 °C with a photoperiod of 12/12 hours). The results indicate that there is a large difference in germination rates between the two populations. The use of KNO3 did not increase germination rates in any case, but differences were found between periods of cold stratification, with six months being the most effective. On the other hand, the germination rates of both populations remain low (17.58% and 2%) compared to the obtained rate of seed viability. With this first approach to the understanding of the germination requirements of C. lainzii, it becomes clear that new essays are needed to obtain better results, in order to effectively implement the protection plans of C. lainzii populations.

scholarly journals Effect of stratification and gibberellic acid on epicotyl dormancy release in seeds of Yunnanopilia longistaminea (Opiliaceae)

2016 ◽  
Author(s):  
Guan-song Yang ◽  
Liu Yang ◽  
Yue-hua Wang ◽  
Shi-kang Shen
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Economic Value ◽  
Cold Stratification ◽  
Dormancy Release ◽  
Spring Temperature ◽  
Edible Plant ◽  
Seed Moisture Content ◽  
Radicle Emergence ◽  
Shoot Emergence

Yunnanopilia longistaminea is an endangered monotypic species belonging to Opiliaceae. This edible plant is an important germplasm source with a high economic value in China. The seed dormancy and germination of Opiliaceae species have been rarely investigated. This study examined the effects of scarification, soaking in gibberellic acid, and dehydration on the seed germination of Y. longistaminea. Results indicated that the seed germination of this species involves two stages: radicle emergence and epicotyls (shoot) emergence. During radicle emergence, the optimum temperatures were 28 °C and 28 °C/20 °C. Seed moisture content and viability decreased as dehydration occurred. Thus, the seeds may be recalcitrant. The optimum GA3 solution for the seeds undergoing shoot emergence was 100 mg·L−1. The percentages of shoot emergence in 7 and 14 days of stratification at 5 °C were slightly higher than those in other groups. This study is the first to describe epicotyl dormancy in Y. longistaminea seeds. From the seed grow to the seedling of Y. longistaminea subjected to a autumn→winter→spring temperature process in nature conditions. Warm and cold stratification can alleviate radicle and epicotyl dormancy, respectively. The duration of cold stratification also significantly affects the epicotyl dormancy release of Y. longistaminea. The researches on the seeds breaking methods: warm(28°C/20°C)→cold(5°C)→GA3(100mg·L−1)→warm(28°C/20°C).

scholarly journals Dormancy and Germination In Vitro Response of Hydrangea macrophylla and Hydrangea paniculata Seed to Light, Cold-Treatment and Gibberellic Acid

2010 ◽  
Vol 28 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Stephen P. Greer ◽  
Timothy A. Rinehart
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Red Light ◽  
Cold Treatment ◽  
Seed Viability ◽  
In Vitro Study ◽  
Potassium Nitrate ◽  
Hydrangea Macrophylla ◽  
Solid Media

Abstract Seed germination was optimized for ten Hydrangea macrophylla (Thunb.) Ser. and two Hydrangea paniculata Siebold cultivars in vitro. Methods were also developed to assay seed physiology. Best results for in vitro study were obtained with 0.5× Gamborgs solid media in conjunction with Plant Preservative Mixture (PPM), and by sterilizing seed with trichloro-s-triazinetrione (Trichlor). Assays of physiology were conducted by sterilizing seed and treating with combinations of white and red light, cold-treatment, gibberellic acid and potassium nitrate, and light cycles. Estimates of seed viability/dormancy, germination of non-dormant seed, and germination overall were calculated for each treatment combination. The most favorable conditions for overall Hydrangea seed germination were cold-treatment for 6 weeks, imbibition with GA3 + KNO3, and plating on half-strength Gamborgs media supplemented with GA3 in the presence of white light.

Sign in / Sign up

Export Citation Format

Share Document