Making normal NGM for imaging plates (Cabreiro Lab) v1

2021 ◽  
Author(s):  
Saul Moore
Keyword(s):  
Growth Medium ◽  
Agar Medium ◽  
Peristaltic Pump ◽  
Nematode Growth Medium ◽  
Constant Amount ◽  
C Elegans ◽  
Assay Design ◽  
Petri Plate ◽  
Imaging Plates

C. elegans is maintained in the laboratory on Nematode Growth Medium (NGM) agar which has been aseptically poured into petri plates. The NGM agar medium can be poured into petri plates easily and aseptically using a peristaltic pump. This pump can be adjusted so that a constant amount of NGM agar is dispensed into each petri plate. A constant amount of agar in the plates reduces the need for refocusing the microscope when you switch from one plate to another. The imaging plates can be 35mm, 60mm or 90mm in diameter depending on the assay design.

Nematode Growth Medium (NGM)

2014 ◽  
Vol 2014 (3) ◽  
pp. pdb.rec081299-pdb.rec081299
Keyword(s):  
Growth Medium ◽  
Nematode Growth Medium

scholarly journals Effects of Vitamin B12 Deficiency on Amyloid-β Toxicity in Caenorhabditis elegans

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 962
Author(s):  
Arif Andra ◽  
Shoko Tanigawa ◽  
Tomohiro Bito ◽  
Atsushi Ishihara ◽  
Fumio Watanabe ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Growth Medium ◽  
Vitamin B12 Deficiency ◽  
Amyloid Β ◽  
Aβ Peptides ◽  
C Elegans ◽  
Aβ Aggregation ◽  
B12 Deficiency ◽  
The Relationship ◽  
Aβ Production

High homocysteine (Hcy) levels, mainly caused by vitamin B12 deficiency, have been reported to induce amyloid-β (Aβ) formation and tau hyperphosphorylation in mouse models of Alzheimer’s disease. However, the relationship between B12 deficiency and Aβ aggregation is poorly understood, as is the associated mechanism. In the current study, we used the transgenic C. elegans strain GMC101, which expresses human Aβ1–42 peptides in muscle cells, to investigate the effects of B12 deficiency on Aβ aggregation–associated paralysis. C. elegans GMC101 was grown on nematode growth medium with or without B12 supplementation or with 2-O-α-D-glucopyranosyl-L-ascorbic acid (AsA-2G) supplementation. The worms were age-synchronized by hypochlorite bleaching and incubated at 20 °C. After the worms reached the young adult stage, the temperature was increased to 25 °C to induce Aβ production. Worms lacking B12 supplementation exhibited paralysis faster and more severely than those that received it. Furthermore, supplementing B12-deficient growth medium with AsA-2G rescued the paralysis phenotype. However, AsA-2G had no effect on the aggregation of Aβ peptides. Our results indicated that B12 supplementation lowered Hcy levels and alleviated Aβ toxicity, suggesting that oxidative stress caused by elevated Hcy levels is an important factor in Aβ toxicity.

scholarly journals Comparison of Agar and an Agitated, Thin-film, Liquid System for Micropropagation of Ornamental Elephant Ears

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 1088-1092 ◽  
Author(s):  
Jeffrey Adelberg ◽  
Joe Toler
Keyword(s):  
Thin Film ◽  
Growth Medium ◽  
Agar Medium ◽  
Dry Weight ◽  
Liquid System ◽  
Multiplication Rate ◽  
Film Culture ◽  
Agar Culture ◽  
Solid Agar ◽  
Semi Solid

Micropropagation of black-stemmed elephant ear (C. esculenta (L.) Schott `Fontanesii')' and upright elephant ear (A. macrorrhizos G. Don) were compared in semi-solid agar media and agitated, liquid thin-film bioreactor vessels at four explant densities (33, 100, 165, and 330 explants/L of media) using two growth regulator combinations: 1) 1 μm benzylaminopurine (BA)—growth medium, and 2) 3 μm BA plus 3 μm ancymidol—multiplication medium. The thin-film liquid system outperformed agar culture for most measured responses. Some exceptions were relative dry weights at higher explant densities and multiplication rate of Colocasia. When the thin-film liquid system was compared to agar culture, Alocasia explants produced their greatest biomass and had the least residual sugar at the highest explant density. Alocasia explants multiplied most rapidly and had the greatest relative dry weight on liquid media at the low explant densities. Alocasia plants were larger in growth medium than multiplication medium and larger in liquid medium than agar medium. When compared to agar, Colocasia in the thin-film liquid system produced the greatest biomass at the highest explant density in growth medium, had the greatest relative dry weight at the lowest explant density, and used the most sugar at the highest explant density. Alocasia and Colocasia would likely produce greater fresh and dry weight at the highest explant density if additional sugar were supplied during thin-film culture. Greater growth in thin-film culture of Alocasia and Colocasia is due in part, to greater availability of sugar in liquid compared to agar medium.

THE CONGO RED REACTION IN BACTERIA AND ITS USEFULNESS IN THE IDENTIFICATION OF RHIZOBIA

1966 ◽  
Vol 12 (4) ◽  
pp. 725-733 ◽  
Author(s):  
N. J. Hahn
Keyword(s):  
Growth Medium ◽  
Congo Red ◽  
Bacterial Cell ◽  
Agar Medium ◽  
Rhizobium Meliloti ◽  
Rhizobium Trifolii ◽  
Selection For ◽  
Negative Form ◽  
Medium Influence

Congo red was found in this research to form colloidal complexes with di-and tri-valent cations in the presence of acid and alkali. The complexes formed at a pH of 2–3 were uniformly blue; those formed at a pH greater than 12 varied in color with the ion involved, a fuchsia complex being formed with magnesium. The congo red reaction in bacteria (e.g. coloration of the growth in media containing the dye) is explained as due to the adsorption of Congo red on the surface of the bacterial cell, to the ions which predominate at the surface, and to the production of acid or alkali by the bacterium during growth. The temperature of incubation and composition of the growth medium influence colony coloration by affecting the metabolism of the organism.A negative form of selection for rhizobia is suggested using a nitrogen-deficient and a nitrogen-rich carbohydrate medium containing 0.25 g Congo red per liter of agar and 0.025 g Congo red per liter of broth, with incubation at 28 °C and 37 °C respectively. Color differentiation among the rhizobial strains tested, four normal and seven mutant varieties of Rhizobium meliloti and Rhizobium trifolii, occurred on the nitrogen-rich agar medium when incubation was carried out at 25 °C to 28 °C.

A Flooded Plate Loop Count Procedure1

1980 ◽  
Vol 43 (7) ◽  
pp. 534-535 ◽  
Author(s):  
R. L. OLSEN ◽  
G. H. RICHARDSON
Keyword(s):  
Correlation Coefficient ◽  
Conventional Method ◽  
Colony Formation ◽  
Agar Medium ◽  
Raw Milk ◽  
Statistical Characteristics ◽  
Milk Samples ◽  
Standard Procedures ◽  
Conventional Plate ◽  
Petri Plate

A modification of the plate loop count procedure for milk samples is described which incorporates use of a 0.5-ml diluent loop-rinse onto pre-dried agar medium in a 100-mm petri plate. The sample is distributed using a glass spreader, the plate is dried and incubated using standard procedures. Colony formation was more uniform than with the conventional method. The method did not appear to significantly change the statistical characteristics of the plate loop count on raw milk samples. A correlation coefficient of .91 was obtained between the flooded and the conventional plate loop count.

Nematode growth medium (NGM)

2008 ◽  
Vol 2008 (11) ◽  
pp. pdb.rec11474-pdb.rec11474 ◽  
Keyword(s):  
Growth Medium ◽  
Nematode Growth Medium

Olfactory response to beetle-produced volatiles and host-food attractants by Oryzaephilus surinamensis and O. mercator

1981 ◽  
Vol 59 (10) ◽  
pp. 1980-1990 ◽  
Author(s):  
A. M. Pierce ◽  
J. H. Borden ◽  
A. C. Oehlschlager
Keyword(s):  
Growth Medium ◽  
Aggregation Pheromone ◽  
Olfactory Response ◽  
Oryzaephilus Surinamensis ◽  
Interspecific Attraction ◽  
Male And Female ◽  
Porapak Q ◽  
Wide Range ◽  
Food Attractants ◽  
Petri Plate

A two-choice, pitfall olfactometer which utilizes a petri plate with suspended glass vials was employed to test the response of Oryzaephilus surinamensis (L.) and O. mercator (Fauvel) to various volatile stimuli. Male and female beetles of both species were attracted to pentane extracts of Porapak Q trapped volatiles from beetles, frass, rolled oats, and brewer's yeast over a wide range of stimulus concentrations. For both species, male and female frass contained attractive volatiles in addition to those from unused growth medium. This result and the attractiveness of volatiles from beetles suggest that both sexes produce an aggregation pheromone. Additionally, interspecific attraction to beetle and frass volatiles was demonstrated.

scholarly journals Influence of temperature and different culture media on growth of fusarium udum (Butler), causal organism of wilt of pigeonpea

2016 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Udaysingh Desai ◽  
Yogesh Andoji ◽  
Shivaji Kamble
Keyword(s):  
Growth Medium ◽  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Pigeon Pea ◽  
Optimum Temperature ◽  
Causal Organism ◽  
Influence Of Temperature ◽  
Fusarium Udum ◽  
Pea Seed

<p>The conditions suitable for growth of Fusarium udum causing wilt of Pigeon pea were analysed. Pigeon pea seed agar medium and Czapek Dox Agar medium was found to be most suitable growth medium for mycelial growth and sporulation of Fusarium udum. The optimum temperature for growth of Fusarium udum was found to be 28±2°C.</p>

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

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