FindingNemo Library 2: Modified RAD004 v1

2021 ◽  
Author(s):  
Inswasti Cahyani ◽  
John Tyson ◽  
Nadine Holmes ◽  
Josh Quick ◽  
Nicholas Loman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Large Volume ◽  
Input Quality ◽  
Adapter Ligation ◽  
Working Principle ◽  
Ultra High Molecular Weight

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocol we tested here is based on ONT's rapid kit, i.e., SQK-RAD004, a transposase based adapter ligation kit. This Modified RAD004 protocol consistently produced N50 > 100 kb from a good input quality of UHMW DNA, for when ULK001 is not accessible/available. Transposase-based reaction is done in a large volume of up to 1 ml. The working principle of the RAD004 kit is shown in the diagram below:

FindingNemo Library 1: Modified ULK001 v1

2021 ◽  
Author(s):  
Inswasti Cahyani ◽  
John Tyson ◽  
Nadine Holmes ◽  
Josh Quick ◽  
Nicholas Loman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Large Volume ◽  
Cost Effective ◽  
Input Quality ◽  
Adapter Ligation ◽  
Working Principle ◽  
Ultra High Molecular Weight

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocols we tested are based on ONT's rapid kit, i.e., SQK-ULK001, a transposase based adapter ligation kit. Modified ULK001 protocol consistently produced N50 > 100 kb from a good input quality of UHMW DNA and is our recommended route for best output as it is also the most-cost effective. Transposase-based reaction is done in a large volume of up to 1 ml. The working principle of the ULK001 protocol is shown in the diagram below:

scholarly journals The Influence of the Use of Polymer Lining within the Roller Press Gravity Feeder on Briquette Quality

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2489
Author(s):  
Michał Bembenek
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Material Flow ◽  
Laboratory Tests ◽  
Polymer Materials ◽  
Roller Press ◽  
Fine Grained ◽  
The Impact ◽  
Ultra High Molecular Weight

When considering the operation of roller presses for the consolidation of fine-grained materials, the main problems are disturbances in the proper flow of the material and its bridging in gravity feeders. This is especially true for small and medium capacity presses, where the hoppers for dosing the material are narrow. This article presents innovative laboratory tests of the impact of using a polymer plate lining in the gravity feeder of a roller press. Polymer materials Polyacetal C (POM C) and Ultra-High-Molecular-Weight Polyethylene (UHMW-PE) were used for the tests. The influence of the use of plates on the material flow and quality of briquettes was investigated in comparison with the case where such plates were not used. The research showed an improvement in the flow of fine-grained materials in the feeder and an increase of the briquette strength indexes, as compared to those cases when polymer linings were not used in the feeder.

FindingNemo Library 3: KrazyStarFish (KSF) v1

2021 ◽  
Author(s):  
John Tyson ◽  
Inswasti Cahyani ◽  
Nadine Holmes ◽  
Josh Quick ◽  
Nicholas Loman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Filter Paper ◽  
Adapter Ligation ◽  
Dna Precipitation ◽  
The Right ◽  
Ultra High Molecular Weight

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocol we tested here is based on ONT's rapid kit, i.e., SQK-RAD004, a transposase based adapter ligation kit. We named this protocol KrazyStarFish (KSF). It offers a different approach to UL library prep, by using filter paper shaped as a starfish at the DNA precipitation/clean-up step. It can consistently produced N50 > 100 kb with the right transposase to DNA ratio. This protocol was developed by John Tyson at UBC, Vancouver.

Using Isostatic Pressing Techniques to Mold Uhmwpe

1995 ◽  
Vol 394 ◽  
Author(s):  
Joel Higgins ◽  
David Schroeder
Keyword(s):  
Molecular Weight ◽  
Quality Control ◽  
High Molecular Weight ◽  
Bearing Material ◽  
Isostatic Pressing ◽  
Manufacturing Methods ◽  
Particulate Debris ◽  
And Performance ◽  
Ultra High Molecular Weight

Ultra High Molecular Weight Polyethylene (UHMWPE) has been and is currently the standard for bearing material used in the orthopedic industry. The components are produced using a variety of manufacturing methods, many of which can have an effect on the longevity and performance of the device. Recently there has been extensive research into the causes of loosening of orthopedic devices. One area that has been targeted as a cause for loosening is reactions to particulate debris from the bearing surfaces of these appliances. As biological reactions to particulate become better understood, there has been an increased emphasis on the quality of the UHMWPE forms used for orthopedic bearing surfaces. Due to this increased awareness, various manufacturing and quality control improvements have been made throughout the industry.

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