FindingNemo Library 3: KrazyStarFish (KSF) v1

2021 ◽  
Author(s):  
John Tyson ◽  
Inswasti Cahyani ◽  
Nadine Holmes ◽  
Josh Quick ◽  
Nicholas Loman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Filter Paper ◽  
Adapter Ligation ◽  
Dna Precipitation ◽  
The Right ◽  
Ultra High Molecular Weight

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocol we tested here is based on ONT's rapid kit, i.e., SQK-RAD004, a transposase based adapter ligation kit. We named this protocol KrazyStarFish (KSF). It offers a different approach to UL library prep, by using filter paper shaped as a starfish at the DNA precipitation/clean-up step. It can consistently produced N50 > 100 kb with the right transposase to DNA ratio. This protocol was developed by John Tyson at UBC, Vancouver.

FindingNemo Library 1: Modified ULK001 v1

2021 ◽  
Author(s):  
Inswasti Cahyani ◽  
John Tyson ◽  
Nadine Holmes ◽  
Josh Quick ◽  
Nicholas Loman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Large Volume ◽  
Cost Effective ◽  
Input Quality ◽  
Adapter Ligation ◽  
Working Principle ◽  
Ultra High Molecular Weight

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocols we tested are based on ONT's rapid kit, i.e., SQK-ULK001, a transposase based adapter ligation kit. Modified ULK001 protocol consistently produced N50 > 100 kb from a good input quality of UHMW DNA and is our recommended route for best output as it is also the most-cost effective. Transposase-based reaction is done in a large volume of up to 1 ml. The working principle of the ULK001 protocol is shown in the diagram below:

FindingNemo Library 2: Modified RAD004 v1

2021 ◽  
Author(s):  
Inswasti Cahyani ◽  
John Tyson ◽  
Nadine Holmes ◽  
Josh Quick ◽  
Nicholas Loman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Large Volume ◽  
Input Quality ◽  
Adapter Ligation ◽  
Working Principle ◽  
Ultra High Molecular Weight

This sub-protocol is designed to prepare library from extracted ultra-high molecular weight (UHMW) DNA to obtain ultra-long (UL) reads on Nanopore sequencers. The UL library protocol we tested here is based on ONT's rapid kit, i.e., SQK-RAD004, a transposase based adapter ligation kit. This Modified RAD004 protocol consistently produced N50 > 100 kb from a good input quality of UHMW DNA, for when ULK001 is not accessible/available. Transposase-based reaction is done in a large volume of up to 1 ml. The working principle of the RAD004 kit is shown in the diagram below:

Application of ultra-high molecular weight polyethylene in a hydraulic drive

2020 ◽  
pp. 77-78
Keyword(s):  
Molecular Weight ◽  
Low Temperature ◽  
High Molecular Weight ◽  
Hydraulic Drive ◽  
Control Valve ◽  
Hydraulic Control ◽  
Hydraulic Motor ◽  
Hydraulic Pump ◽  
Hydraulic Oil ◽  
Ultra High Molecular Weight

The use of ultra-high molecular weight polyethylene (UHMW PE) for the manufacture of various parts, in particular cuffs for hydraulic drives, is proposed. The properties and advantages of UHMW PE in comparison with other polyethylene materials are considered. Keywords ultra-high molecular weight polyethylene, hydraulic pump, hydraulic motor, hydraulic control valve, hydraulic oil, low temperature. [email protected]

scholarly journals Structure, processing and performance of ultra-high molecular weight polyethylene (IUPAC Technical Report). Part 4: sporadic fatigue crack propagation

2020 ◽  
Vol 92 (9) ◽  
pp. 1521-1536
Author(s):  
Clive Bucknall ◽  
Volker Altstädt ◽  
Dietmar Auhl ◽  
Paul Buckley ◽  
Dirk Dijkstra ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Fatigue Crack ◽  
Crack Propagation ◽  
High Molecular Weight ◽  
Fatigue Crack Propagation ◽  
Crack Tip ◽  
Individual Growth ◽  
Paris Equation ◽  
Number Of Cycles ◽  
Ultra High Molecular Weight

AbstractFatigue tests were carried out on compression mouldings supplied by a leading polymer manufacturer. They were made from three batches of ultra-high molecular weight polyethylene (UHMWPE) with weight-average relative molar masses, ${\overline{M}}_{\mathrm{W}}$, of about 0.6 × 106, 5 × 106 and 9 × 106. In 10 mm thick compact tension specimens, crack propagation was so erratic that it was impossible to follow standard procedure, where crack-tip stress intensity amplitude, ΔK, is raised incrementally, and the resulting crack propagation rate, da/dN, increases, following the Paris equation, where a is crack length and N is number of cycles. Instead, most of the tests were conducted at fixed high values of ΔK. Typically, da/dN then started at a high level, but decreased irregularly during the test. Micrographs of fracture surfaces showed that crack propagation was sporadic in these specimens. In one test, at ΔK = 2.3 MPa m0.5, there were crack-arrest marks at intervals Δa of about 2 μm, while the number of cycles between individual growth steps increased from 1 to more than 1000 and the fracture surface showed increasing evidence of plastic deformation. It is concluded that sporadic crack propagation was caused by energy-dissipating crazing, which was initiated close to the crack tip under plane strain conditions in mouldings that were not fully consolidated. By contrast, fatigue crack propagation in 4 mm thick specimens followed the Paris equation approximately. The results from all four reports on this project are reviewed, and the possibility of using fatigue testing as a quality assurance procedure for melt-processed UHMWPE is discussed.

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