Design and preparation of synthetic reference peptides for APP/Aβ TOMAHAQ proteomics v1
TOMAHAQ-based targeted proteomics relies on heavy-labeled reference peptides for multi-plexed quantification of peptides of interest within a set of samples. The APP amyloid precursor protein is thought to be proteolytically processed within the endolysosomal system by β-secretase and ϒ-secretase to yeild various forms of Aβ. To quantitatively track APP products, we describe a protocol for design and generation of synthetic reference peptides for TOMAHAQ analysis. We also include reference peptides for the extracellular and cytosolic domains.
2006 ◽
Vol 26
(2)
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pp. 418-428
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